Search Results (5,087)

The global ecosystem relies on the metabolism of photosynthetic organisms, featuring the ability to harness light as an energy source. The most successful type of photosynthesis utilizes a virtually inexhaustible electron pool from water, but the driver of this oxidation, sunlight, varies on time and intensity scales of several orders of magnitude. Such rapid and steep changes in energy availability are potentially devastating for biological systems. To enable a safe and efficient light-harnessing process, photosynthetic organisms tune their light capturing, the redox connections between core complexes and auxiliary electron mediators, ion passages across the membrane, and functional coupling of energy transducing organelles. Here, microalgal species are the most diverse group, featuring both unique environmental adjustment strategies and ubiquitous protective mechanisms. In this review, we explore a selection of regulatory processes of the microalgal photosynthetic apparatus supporting smooth electron flow in variable environments. Full article

TNF-α-induced protein 3 (TNFAIP3), commonly referred to as A20, is an integral part of the ubiquitin-editing complex that significantly influences immune regulation, apoptosis, and the initiation of diverse immune responses. The A20 protein is characterized by an N-terminal ovarian tumor (OTU) domain and a series of seven zinc finger (ZNF) domains. Mutations in the TNFAIP3 gene are implicated in various immune-related diseases, such as Behçet’s disease, polyarticular juvenile idiopathic arthritis, autoimmune thyroiditis, autoimmune hepatitis, and rheumatoid arthritis. These mutations can lead to a spectrum of symptoms, including, but not limited to, recurrent fever, ulcers, rashes, musculoskeletal and gastrointestinal dysfunctions, cardiovascular issues, and respiratory infections. The majority of these mutations are either nonsense (STOP codon) or frameshift mutations, which are typically associated with immune dysfunctions. Nonetheless, missense mutations have also been identified as contributors to these conditions. These genetic alterations may interfere with several biological pathways, notably abnormal NF-κB signaling and dysregulated ubiquitination. Currently, there is no definitive treatment for A20 haploinsufficiency; however, therapeutic strategies can alleviate the symptoms in patients. This review delves into the mutations reported in the TNFAIP3 gene, the clinical progression in affected individuals, potential disease mechanisms, and a brief overview of the available pharmacological interventions for A20 haploinsufficiency. Mandatory genetic testing of the TNFAIP3 gene should be performed in patients diagnosed with autoinflammatory disorders to better understand the genetic underpinnings and guide treatment decisions. Full article

Objectives: Oral diseases are among the most prevalent diseases globally. Accumulating new evidence suggests considerable benefits of epigallocatechin-3-gallate (EGCG) for oral health. This review aims to explore the role and application of EGCG in main oral diseases. Methods: This narrative review thoroughly examines and summarizes the most recent literature available in scientific databases (PubMed, Web of Science, Scopus, and Google Scholar) reporting advances in the role and application of EGCG within the dental field. The major keywords used included “EGCG”, “green tea extract”, “oral health”, “caries”, “pulpitis”, “periapical disease”, “periodontal disease”, “oral mucosa”, “salivary gland”, and “oral cancer”. Conclusions: EGCG prevents and manages various oral diseases through its antibacterial, anti-inflammatory, antioxidant, and antitumor properties. Compared to traditional treatments, EGCG generally exhibits lower tissue irritation and positive synergistic effects when combined with other therapies. Novel delivery systems or chemical modifications can significantly enhance EGCG’s bioavailability, prolong its action, and reduce toxicity, which are current hotspots in developing new materials. Clinical significance: this review provides an exhaustive overview of the biological activities of EGCG to major oral diseases, alongside an exploration of applications and limitations, which serves as a reference for preventing and managing oral ailments. Full article

The microbial genus Bacillus inhabits a diverse range of environments and is widespread across all global biomes, with a significant presence in soil habitats. In agriculture, Bacillus strains play multifaceted roles, serving as biocontrol agents against pests and diseases, and promoting plant growth by facilitating nutrient availability and enhancing stress tolerance. Through mechanisms such as phosphate solubilization, ACC-deaminase activity, and synthesis of phytohormones and siderophores, Bacillus spp. contribute to soil health and crop productivity, in a new approach of regenerative agriculture. The ability of Bacillus spp. to solubilize phosphate makes essential nutrients more accessible to plants, while ACC-deaminase activity helps plants withstand environmental stresses. Additionally, the synthesis of phytohormones can stimulate plant growth and development, and siderophores may facilitate the uptake of nutrients such as iron by plants. As the agricultural industry embraces Bacillus-based formulations for pest management and crop enhancement, future research holds promising prospects for optimizing their applications and harnessing their full potential in agroecosystems. Continued exploration of Bacillus spp. diversity and their interactions with plants and soil microbiota will further advance sustainable agricultural practices. This review contributes to understanding how Bacillus strains can revolutionize agriculture by enhancing soil health, increasing crop productivity, and providing effective biological solutions against pests and diseases. The successful application of Bacillus-based technologies in millions of hectares in Brazilian agriculture demonstrates the synergy between the need for more sustainable agricultural practices and the use of bio-inputs. Full article

In contrast to plants, humans are unable to synthesise carotenoids and have to obtain them from diet. Carotenoids fulfil several crucial biological functions in the organism; however, due to poor solubility in water, their bioavailability from plant-based food is low. The processes of carotenoid absorption and availability in the human body have been intensively studied. The recent experimental findings concerning these processes are briefly presented in the introductory part of this review, together with a summary of such topics as carotenoid carriers, body transport and tissue delivery, to finally report on molecular-level studies of carotenoid binding by membrane receptors. The main message of the review is contained in the section describing computational investigations of carotenoid intercalation and dynamic behaviour in lipid bilayers. The relevance of these computational studies lies in showing the direct link between the microscopic behaviour of molecules and the characteristics of their macroscopic ensembles. Furthermore, studying the interactions between carotenoids and lipid bilayers, and certainly proteins, on the molecular- and atomic-level using computational methods facilitates the interpretation and explanation of their macroscopic properties and, hopefully, helps to better understand the biological functions of carotenoids. Full article

The effect of ionizing radiation on cells is a complex process dependent on several parameters. Cancer treatment commonly involves the use of radiotherapy. In addition to the effective killing of cancer cells, another key aspect of radiotherapy is the protection of healthy cells. An interesting position is occupied by heavy ion radiation in the field of radiotherapy due to its high relative biological effectiveness, making it an effective method of treatment. The high biological efficiency of heavy ion radiation can also pose a danger to healthy cells. The extent of cell death induced by heavy ion radiation in cells was investigated using statistical learning methods in this study. The objective was to predict the healthy cell survival rate based on the physical parameters of the available ionizing radiation. This paper is based on secondary research utilizing the PIDE database. Throughout this study, a local regression and a random forest model were generated. Their predictions were compared to the results of a linear-quadratic model commonly utilized in the field of ionizing radiation using various metrics. The relationship between dose and cell survival rate was examined using the linear-quadratic (LQM) model and local regression (LocReg). An R² value of 88.43% was achieved for LQM and 89.86% for LocReg. Upon incorporating linear energy transfer, the random forest model attained an R² value of 96.85%. In terms of RMSE, the linear-quadratic model yielded 9.5910⁻², the local regression 9.2110⁻², and the random forest 1.96 × 10⁻² (lower values indicate better performance). All of these methods were also applied to a log-transformed dataset to decrease the right skewedness of the distribution of the datapoints. This significantly reduced the estimates made with LQM and LocReg (28% decrease in the case of R²), while the random forest retained nearly the same level of estimation as the untransformed data. In conclusion, it can be inferred that dose alone provides a somewhat satisfactory explanatory power for cell survival rate, but the inclusion of linear energy transfer can significantly enhance prediction accuracy in terms of variance and explanatory power. Full article

Liver disease is a global health problem that affects the well-being of tens of thousands of people. Dihydroquercetin (DHQ) is a flavonoid compound derived from various plants. Furthermore, DHQ has shown excellent activity in the prevention and treatment of liver injury, such as the inhibition of hepatocellular carcinoma cell proliferation after administration, the normalization of oxidative indices (like SOD, GSH) in this tissue, and the down-regulation of pro-inflammatory molecules (such as IL-6 and TNF-α). DHQ also exerts its therapeutic effects by affecting molecular pathways such as NF-κB and Nrf2. This paper discusses the latest research progress of DHQ in the treatment of various liver diseases (including viral liver injury, drug liver injury, alcoholic liver injury, non-alcoholic liver injury, fatty liver injury, and immune liver injury). It explores how to optimize the application of DHQ to improve its effectiveness in treating liver diseases, which is valuable for preparing potential therapeutic drugs for human liver diseases in conjunction with DHQ. Full article

Fish are one of the main sources of protein in Cambodia but they are highly perishable. This requires immediate consumption or processing for later use. In processing, fish drying is very common, but most processors practice traditional drying methods although solar dryers have been introduced, or gradually used, in Cambodia. There is a large variation in terms of drying efficiency due to large differences in solar radiation, temperature, and humidity conditions in traditional drying methods and solar dryers. However, there is limited information on the actual variation in these two systems, which should be documented in Cambodia. Using sensors to monitor micro-climatic changes inside the drying chamber will be useful to improve efficiency and performance. Therefore, the objectives of this research were to (1) design a fish dryer from locally available inputs; (2) determine changes in solar radiation over time; (3) compare relative humidity and temperatures between traditional sun-drying and the solar dryer; (4) determine the relationship among the climatic parameters; and (5) compare some physical, chemical, and biological properties of dried fish in both drying techniques with the Cambodian dried fish standards. The study was conducted in collaboration with a fish processor in the Siem Reap Province between December 2023 and January 2024 using a sensor-mounted solar dryer fabricated by the Royal University of Agriculture to dry fish and compared with traditional sun-drying. Three experiments were carried out from 8:00 to 16:00 following the common drying practices in Cambodia. In each experiment, 80–100 kg of raw giant snakehead, or 56–70 kg of prepared fish (1.04 ± 0.05 kg each fish), was prepared for drying. Data on environmental conditions were measured and analyzed. The results show that the solar dryer had higher temperatures (almost 60 °C) and lower relative humidity (about 20%) during peak hours when compared with traditional sun-drying (36.8 °C and 40%, respectively). In all cases, relative humidity decreased with rising solar radiation and temperatures. The final weight and moisture of dried fish in the solar dryer were lower than those in traditional sun-drying in eight hours. Salmonella was detected with traditional sun-drying but E. coli was not. Bacterial presence may be harmful to human health. Nevertheless, the time spent for drying in both techniques was the same, so future studies should focus on improving ventilation to remove moisture faster out of the solar dryer, which can help with faster drying and more time saving. Hybrid solar dryers should also be considered to maintain high temperatures at night, while bacteria should be counted for safety reasons. Full article

Purpose. This paper aims to present a unique perspective that emphasizes the intricate interplay between energy, dietary proteins, and amino acid composition, underscoring their mutual dependence for health-related considerations. Energy and protein synthesis are fundamental to biological processes, crucial for the sustenance of life and the growth of organisms. Methods and Results. We explore the intricate relationship between energy metabolism, protein synthesis, regulatory mechanisms, protein sources, amino acid availability, and autophagy in order to elucidate how these elements collectively maintain cellular homeostasis. We underscore the vital role this dynamic interplay has in preserving cell life. Conclusions. A deeper understanding of the link between energy and protein synthesis is essential to comprehend fundamental cellular processes. This insight could have a wide-ranging impact in several medical fields, such as nutrition, metabolism, and disease management. Full article

Background: Although, in most children with asthma, good symptom control is achieved with a low to moderate dose of inhaled corticosteroids, a small group of patients still experiences frequent symptoms, and even severe exacerbations, impairment of lung function, and reduced quality of life. Some of these subjects with severe asthma require biologic drugs as add-on therapy. In the past decade, numerous monoclonal antibodies have been approved for children or adolescents with severe asthma, in addition to their increasing use in adult asthma. However, the available evidence on how to select the most appropriate biologic based on a single patient’s clinical, functional, and laboratory characteristics is still scant, and is insufficient to guide clinicians in the decision-making process of a personalized treatment. Materials and Methods: We report a case series of four patients with severe eosinophilic asthma treated with mepolizumab, an anti-interleukin-5 monoclonal antibody, and review the existing literature on this treatment in children and adolescents. Results: Our patients, all with blood eosinophilia and elevated fractional exhaled nitric oxide levels, developed poor symptom control despite prolonged treatment with high-dose inhaled corticosteroids plus a second controller, addressing the addition of a biologic drug. In all of them, a 12-month treatment with subcutaneous mepolizumab showed a reduction in the blood eosinophil count and in asthma exacerbations, as well as an improvement on the Asthma Control Test. The results of the literature search focused on the strengths and limitations of the pediatric use of mepolizumab and highlighted the areas worthy of further research. Conclusions: Mepolizumab has proven effective in improving symptom control in pediatric patients with severe asthma. Additional well-powered clinical trials will be helpful in developing evidence-based guidelines regarding biologic drugs in the pediatric population. Full article

Cestrum parqui L’Herit. (Solanaceae family) is a species of forest shrub, self-incompatible and specialized in pollination, widespread in the subtropical area of the planet, and now widely distributed also in the Mediterranean area. The constituents of its leaves have antimicrobial, anticancer, insecticidal, antifeedant, molluscicidal, and herbicidal properties. The spread of this species represents a valuable source of compounds with high biological value. Various research groups are engaged in defining the chemical composition of the different parts of the plant and in defining its properties in view of important and promising commercial applications. To date, there are only a few incomplete reports on the potential applications of C. parqui extracts as selective natural pesticides and on their potential phytotoxic role. Scientific knowledge and the use of extraction techniques for these components are essential for commercial applications. This article summarizes the research and recent studies available on the botany, phytochemistry, functional properties, and commercial applications of C. parqui. Full article

Cover crops (CCs) are regarded as beneficial to agricultural practice as an option for soil quality improvement in field production systems. The main goal of this study was to assess the impact of spring phacelia (Phacelia tanacetifolia Benth.) and buckwheat (Fagopyrum Mill.) in a crop rotation (CC–leek–parsley, 2020–2021) on the physicochemical and biological properties of the soil in an organic high tunnel system. Soil analyses involved measurements of bulk density, water capacity, soil aggregation, soil organic carbon (SOC), available soil nutrients, as well as microbial abundance and diversity. Phacelia generated more aboveground biomass (58.2 t fresh matter ha⁻¹) than buckwheat (33.0 t ha⁻¹), and their biomass contained 161 kg N ha⁻¹ and 67 kg N ha⁻¹, respectively. A large quantity of elements, such as N, Ca, P, S, B, and Cu, were found in phacelia biomass. More Mg and Na were found in buckwheat plants. The results showed that CC biomass significantly improved some of the soil physical and chemical properties, such as soil organic carbon stock and wet aggregate stability, and decreased soil bulk density. Cover crop treatments changed the dynamics of soil bacterial and fungus populations in a high tunnel system. Phacelia increased the quantity of ammonifiers and nitrifiers in the soil substantially. Further research with a long-term focus is needed to assess the impact of cover crops on soil properties, soil quality, and subsequent crop yields in high tunnel crop rotation and management systems. Full article

Alzheimer’s Disease is among the major chronic neurodegenerative diseases that affects more than 50 million people worldwide. This disease irreversibly destroys memory, cognition, and the overall daily activities which occur mainly among the elderly. Few drugs are approved for Alzheimer’s Disease management despite its high prevalence. To date, the available drugs in the market cannot reverse the damage of neurons caused by the disease leading to the exacerbation of symptoms and possibly death. Medicinal plants are considered a rich source of chemical constituents and have been contributing to modern drug discovery in many therapeutic areas including cancer, infectious, cardiovascular, neurodegenerative and Central Nervous System (CNS) diseases. Moreover, essential oils that are extracted from plant organs have been reported for a wide array of biological activities, and their roles as antioxidants, antiaging, cytotoxic, anti-inflammatory, antimicrobial, and enzyme inhibitory activities. This article highlights the promising potential of plants’ essential oils in the discovery of novel therapeutic options for Alzheimer’s Disease and halting its progression. In this article, 428 compounds were reported from the essential oils isolated from 21 plants. A comparative study is carried out by employing a variety of machine learning techniques, validation, and evaluation metrics, to predict essential oils’ efficacy against Alzheimer’s Disease progression. Extensive experiments on essential oil data suggest that a prediction accuracy of up to 82% can be achieved given the proper data preprocessing, feature selection, and model configuration steps. This study underscores the potential of integrating machine learning with natural product research to prioritize and expedite the identification of bioactive essential oils that could lead to effective therapeutic interventions for Alzheimer’s Disease. Further exploration and optimization of machine learning techniques could provide a robust platform for drug discovery and development, facilitating faster and more efficient screening of potential treatments. Full article

Hydraulic fracturing, or fracking, requires large amounts of water to extract fossil fuel from rock formations. As a result of hydraulic fracturing, the briny wastewater, often termed back-produced fracturing or fracking water (FW), is pumped into holding ponds. One of the biggest challenges with produced water management is controlling microbial activity that could reduce the pond water’s reusable layer and pose a significant environmental hazard. This study focuses on the characterization of back-produced water that has been hydraulically fractured using chemical and biological analysis and the development of a high-throughput screening method to evaluate and predict the antimicrobial effect of four naturally and commercially available acidic inhibitors (edetic acid, boric acid, tannic acid, and lactic acid) on the growth of the FW microbiome. Liquid cultures and biofilms of two laboratory model strains, the vegetative Escherichia coli MG1655, and the spore-forming Bacillus atrophaeus (also known as Bacillus globigii, BG) bacteria, were used as reference microorganisms. Planktonic bacteria in FW were more sensitive to antimicrobials than sessile bacteria in biofilms. Spore-forming BG bacteria exhibited more sensitivity to acidic inhibitors than the vegetative E. coli cells. Organic acids were the most effective bacterial growth inhibitors in liquid culture and biofilm. Full article

Natural soil and vegetation recovery following human disturbance is the primary means of restoring degraded ecosystems globally. However, it remains unclear how vegetation recovery in the tropical karst areas of China affects the soil physicochemical properties. Here, we investigated the impacts of natural vegetation recovery on soil physicochemical properties at different soil depths in tropical karst areas in southwestern China, using a space–time substitution method. We found that with the natural vegetation recovery, soil bulk density (SBD) decreased. Soil pH initially decreased and then increased, reaching its lowest value during the shrubland stage. There was a significant increase in other soil physicochemical factors. In the soil profiles, SBD tended to increase with depth. The pH, total potassium (TK), total phosphorus (TP), and exchangeable calcium remained relatively stable across the different soil layers. TK, TP, available phosphorus, SBD, total nitrogen, pH, exchangeable magnesium, and available potassium significantly contributed to the soil physicochemical properties. Soil physicochemical properties were predominantly directly affected by litter and biological factors, albeit indirectly influenced by topographic factors. Our study provides crucial insights into karst soils and their relationship with vegetation recovery, which are pivotal for steering vegetation restoration and soil amelioration in karst areas. Full article