In-Situ Comparative Study of Eucalyptus, Basil, Cloves, Thyme, Pine Tree, and Tea Tree Essential Oil Biocide Efficacy
Abstract
:1. Introduction
2. Materials and Methods
2.1. FTIR Analysis
2.2. Ultraviolet Induced Luminescence (UVL)
2.3. Spectro-Colorimetric Analysis
2.4. Adenosine Triphosphate (ATP) Test
3. Results and Discussions
3.1. Analysis of Residue Using FTIR Analysis
3.2. Ultraviolet Induced Luminescence (UVL)
3.3. Spectro Colorimetric Analysis
3.4. Adenosine Triphosphate (ATP) Test
4. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Zones | Essential Oils | Composition | pH (Sd < 0.4) |
---|---|---|---|
A | Eucalyptus globulus | EO, NEVEK, Tween20 | 6.0 |
B | Ocimum basilcum | EO, NEVEK, Tween20 | 6.0 |
C | Essenzio | Extract of Thymus vulgaris and Origanum vulgare | 4.6 |
D | Eugenia caryophyllus | EO, NEVEK, Tween20 | 6.0 |
E | YOCOCOIL | NEVEK, Tween20, Pinus cembra L., Eucalyptus globulus, Thymus vulgaris, Eugenia caryophyllus, Ocimum basilicum | 6.4 |
F | Biotersus | Cinnamonum zeylanicum, Eugenia caryophyllata, Corydothymus capitatus, Tween20 | 4.5 |
G | Thymus vulgaris | EO, NEVEK, Tween20 | 6.5 |
H | Pinus cembra L. | EO, NEVEK, Tween20 | 6.5 |
I | Melaleuca alternifolia | EO, NEVEK, Tween20 | 6.5 |
P | Preventol® RI50 | Quaternary ammonium salts | 6.7 |
Biocides | Bands | Bands | Bands |
---|---|---|---|
Without Thickener (after Drying) | With Thickener (before Drying) | With Thickener (after Drying) | |
Eucalyptus | 2928 | 2966-2943-2880-1464-1446-1359-1271-1233-1214-1166-1015-984-887-814-788-645 | 2927-1448-1370-1172-862 |
Basil | 2925-1721-1511-1455-903 | 3077-3001-2963-2910-2834-1611-1584-1510-1463-1439-1300-1243-1176-1110-994-810-623 | 3417-2971-2439-2420-2322-2297-2182-2165-2108-2065-2022-1986-1917-1620-1462-1210-1174-1132-1091-946-859-820-806-788-742-699-683 |
Cloves | 1765-1510 | 3521-3075-3003-2937-2842-1764-1606-1510-1462-1431-1265-1231-1199-1121-994-850-817-793-745-647 | 3425-2971-2896-2288-2190-2164-2050-1982-1457-1410-1263-876 |
YOCOCOIL | 3454-2961-2919-2855- 2149-2048-2030-2002- 1976-1960-1735-1607-1511-1455-1351-1269-1249-1102-999-949-852-706-642-625 | 3348-2962-2924-1511-1449-1432-1267-1244-1214-1176-1121-989-840-807-741 | 3399-2971-2893-2325-2290-2189-2166-2105-2064-2050-1984-1563-1454-1263-1014-875-818-730-676 |
Biotersus | 3338-2051 | 3399-2957-2924-2869-1734-1624-1513-1452-1429-1266-1234-1207-1178-1120-993-943-865-814-796-642 | 3364-2920-2322-2190-2166-2111-2077-2047-1983-1450-1354-1255-1019-888-818-658-631 |
Thyme | 2959-2926-1457 | 3424-2960-2925-2869-1702-1619-1583-1519-1457-1419-1338-1289-1259-1224-1180-1112-1087-997-945-857-808-738 | 2960-2926-1457-1618-1583-1291-1257-809-740 |
Pine tree | 3394-2929-1621-1446- 1379-936 | 2958-2909-2853-2725-1715-1688-1674-1624-1445-1377-1362-1300-1215-1182-1115-1022-1009-980-936-885-867-821-653 | 2921-1622-1446-1378-937 |
Tea tree | 3340-889-864-799 | 3339-995-889-864-830-815-799-780 | 3340 |
Area 1 | Area 2 | |
---|---|---|
Before the treatment | | |
After the biocide treatment | | |
After the chemical and mechanical cleaning | | |
Before the Biocide Treatment | After the Biocide Treatment | |
---|---|---|
Area 1 | | |
Area 2 | | |
Efficacy | P | A | B | C | D | E | F | G | H | I |
---|---|---|---|---|---|---|---|---|---|---|
Area 1 | ** | * | * | *** | ** | *** | ** | *** | *** | * |
Area 2 | *** | *** | ** | * | * | ** | ** | *** | * | ** |
Biocides | Area 1 | Area 2 | |||||||
---|---|---|---|---|---|---|---|---|---|
Before | After | Difference | SD | Before | After | Difference | SD | ||
X | Chemical and mechanical treatment | 54,678 | 9327 | 45,351 | <1102 | 75,346 | 8800 | 66,546 | 1310 |
P | Preventol® RI50 | 57,617 | 14,261 | 43,356 | <1772 | 71,971 | 11,896 | 60,075 | 1001 |
A | Eucalyptus | 48,765 | 26,291 | 22,474 | <1652 | 62,113 | 29,231 | 32,882 | 1641 |
B | Basil | 54,891 | 25,447 | 29,444 | <1586 | 78,830 | 27,001 | 51,829 | 896 |
C | Essenzio | 63,375 | 32,542 | 30,833 | <3206 | 75,344 | 31,244 | 44,100 | 1442 |
D | Cloves | 61,932 | 34,341 | 27,591 | <1188 | 61,344 | 23,789 | 37,555 | 1204 |
E | YOCOCOIL | 53,168 | 13,351 | 39,817 | <2325 | 56,793 | 16,101 | 40,692 | 2412 |
F | Biotersus | 54,084 | 19,538 | 34,546 | <2988 | 60,668 | 19,567 | 41,101 | 2191 |
G | Thyme | 44,263 | 17,311 | 26,952 | <692 | 62,231 | 24,878 | 37,353 | 1380 |
H | Pine tree | 50,442 | 23,945 | 26,497 | <1247 | 60,323 | 26,578 | 33,745 | 1567 |
I | Tea tree | 35,261 | 19,321 | 15,940 | <1281 | 60,641 | 24,080 | 36,561 | 2156 |
Biocides | Area 1 | Area 2 | ||||||
---|---|---|---|---|---|---|---|---|
Vis | UV | CIELab | ATP | Vis | UV | CIELab | ATP | |
Preventol® RI50 | x | x | xx | x | x | x | xx | |
Eucalyptus | x | |||||||
Basil | x | |||||||
Essenzio | x | x | ||||||
Cloves | ||||||||
YOCOCOIL | x | x | x | x | x | x | x | |
Biotersus | x | x | x | |||||
Thyme | x | x | x | |||||
Pine tree | x | x | ||||||
Tea tree | x | x | x |
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Macchia, A.; Aureli, H.; Prestileo, F.; Ortenzi, F.; Sellathurai, S.; Docci, A.; Cerafogli, E.; Colasanti, I.A.; Ricca, M.; La Russa, M.F. In-Situ Comparative Study of Eucalyptus, Basil, Cloves, Thyme, Pine Tree, and Tea Tree Essential Oil Biocide Efficacy. Methods Protoc. 2022, 5, 37. https://doi.org/10.3390/mps5030037
Macchia A, Aureli H, Prestileo F, Ortenzi F, Sellathurai S, Docci A, Cerafogli E, Colasanti IA, Ricca M, La Russa MF. In-Situ Comparative Study of Eucalyptus, Basil, Cloves, Thyme, Pine Tree, and Tea Tree Essential Oil Biocide Efficacy. Methods and Protocols. 2022; 5(3):37. https://doi.org/10.3390/mps5030037
Chicago/Turabian StyleMacchia, Andrea, Hélène Aureli, Fernanda Prestileo, Federico Ortenzi, Shaila Sellathurai, Antonella Docci, Eleonora Cerafogli, Irene Angela Colasanti, Michela Ricca, and Mauro Francesco La Russa. 2022. "In-Situ Comparative Study of Eucalyptus, Basil, Cloves, Thyme, Pine Tree, and Tea Tree Essential Oil Biocide Efficacy" Methods and Protocols 5, no. 3: 37. https://doi.org/10.3390/mps5030037