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Microbial Enzymes for Biotechnological Applications: 2nd Edition

A special issue of International Journal of Molecular Sciences (ISSN 1422-0067). This special issue belongs to the section "Biochemistry".

Deadline for manuscript submissions: 20 February 2025 | Viewed by 2068

Special Issue Editor


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Guest Editor
Department of Biotechnology, University of Verona, 37134 Verona, Italy
Interests: industrial enzymology; extremozymes; biocatalysis; biorefinery; biomass valorisation; enzymatic recycling of plastics; protein engineering; CRISPR-based applications; virus biotechnology
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Special Issue Information

Dear Colleagues,

This Special Issue is a continuation of our previous Special Issue “Microbial Enzymes for Biotechnological Applications”. Microbial biocatalysts account for about 90% of the enzyme market shares. This mainly depends on the rapid multiplication, easy manipulation, and handling of the microbial cell factories if compared to other biocatalysts sources (i.e., animal, and vegetal tissues). Unlike chemical catalysts, enzymes show wider application potentials that are revolutionizing the agricultural, chemical, energy, and pharmaceutical industries.

The aim is to collect original research articles, review articles, and short communications dealing with the study of microbial biocatalysts (both isolated enzymes and whole-cell biocatalysts). We welcome fundamental studies about design and optimization of microbial enzymes as well as those addressed to the exploitation of microbial enzymes for biotechnological applications (industrial, diagnostic, environmental, etc.).

Topics of interest include, but are not limited to, the following:

  • Discovery and/or characterization of new microbial enzymes (including commodity, specialty, extremophilic and polyextremophilic enzymes);
  • Functional and/or structural characterization of microbial enzymes;
  • Microbial enzymes for circular economy applications;
  • Design, optimization, and/or exploitation of whole-cell microbial biocatalysts;
  • Bioprospecting of microbial enzymes.

Dr. Salvatore Fusco
Guest Editor

Manuscript Submission Information

Manuscripts should be submitted online at www.mdpi.com by registering and logging in to this website. Once you are registered, click here to go to the submission form. Manuscripts can be submitted until the deadline. All submissions that pass pre-check are peer-reviewed. Accepted papers will be published continuously in the journal (as soon as accepted) and will be listed together on the special issue website. Research articles, review articles as well as short communications are invited. For planned papers, a title and short abstract (about 100 words) can be sent to the Editorial Office for announcement on this website.

Submitted manuscripts should not have been published previously, nor be under consideration for publication elsewhere (except conference proceedings papers). All manuscripts are thoroughly refereed through a single-blind peer-review process. A guide for authors and other relevant information for submission of manuscripts is available on the Instructions for Authors page. International Journal of Molecular Sciences is an international peer-reviewed open access semimonthly journal published by MDPI.

Please visit the Instructions for Authors page before submitting a manuscript. There is an Article Processing Charge (APC) for publication in this open access journal. For details about the APC please see here. Submitted papers should be well formatted and use good English. Authors may use MDPI's English editing service prior to publication or during author revisions.

Keywords

  • microbial enzymes
  • whole-cell biocatalysts
  • extremozyme
  • enzyme assays
  • protein engineering

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Published Papers (1 paper)

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Review

51 pages, 6029 KiB  
Review
Microbial Immobilized Enzyme Biocatalysts for Multipollutant Mitigation: Harnessing Nature’s Toolkit for Environmental Sustainability
by Mohamed A. A. Abdelhamid, Hazim O. Khalifa, Hyo Jik Yoon, Mi-Ran Ki and Seung Pil Pack
Int. J. Mol. Sci. 2024, 25(16), 8616; https://doi.org/10.3390/ijms25168616 - 7 Aug 2024
Viewed by 1369
Abstract
The ever-increasing presence of micropollutants necessitates the development of environmentally friendly bioremediation strategies. Inspired by the remarkable versatility and potent catalytic activities of microbial enzymes, researchers are exploring their application as biocatalysts for innovative environmental cleanup solutions. Microbial enzymes offer remarkable substrate specificity, [...] Read more.
The ever-increasing presence of micropollutants necessitates the development of environmentally friendly bioremediation strategies. Inspired by the remarkable versatility and potent catalytic activities of microbial enzymes, researchers are exploring their application as biocatalysts for innovative environmental cleanup solutions. Microbial enzymes offer remarkable substrate specificity, biodegradability, and the capacity to degrade a wide array of pollutants, positioning them as powerful tools for bioremediation. However, practical applications are often hindered by limitations in enzyme stability and reusability. Enzyme immobilization techniques have emerged as transformative strategies, enhancing enzyme stability and reusability by anchoring them onto inert or activated supports. These improvements lead to more efficient pollutant degradation and cost-effective bioremediation processes. This review delves into the diverse immobilization methods, showcasing their success in degrading various environmental pollutants, including pharmaceuticals, dyes, pesticides, microplastics, and industrial chemicals. By highlighting the transformative potential of microbial immobilized enzyme biocatalysts, this review underscores their significance in achieving a cleaner and more sustainable future through the mitigation of micropollutant contamination. Additionally, future research directions in areas such as enzyme engineering and machine learning hold immense promise for further broadening the capabilities and optimizing the applications of immobilized enzymes in environmental cleanup. Full article
(This article belongs to the Special Issue Microbial Enzymes for Biotechnological Applications: 2nd Edition)
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