Advances in Cultured Meat Science and Technology

A special issue of Foods (ISSN 2304-8158). This special issue belongs to the section "Meat".

Deadline for manuscript submissions: 28 February 2025 | Viewed by 3854

Special Issue Editor


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Guest Editor
Department of Food Science and Biotechnology of Animal Resources, Konkuk University, Seoul 05029, Republic of Korea
Interests: meat science; meat product; meat analog; cultivated meat; milk science; dairy products; toxicology; functional foods

Special Issue Information

Dear Colleagues,

In the face of a growing global population, securing a reliable protein supply becomes increasingly crucial. While plant-based protein sources are abundant, animal meat remains a preferred, high-quality protein option. The emergence of cultured meat, also known as in vitro meat, represents a promising avenue in meat analog production through cell culture technology. This innovation may bring benefits such as environmental sustainability, disease prevention and enhanced animal welfare. Although still an emerging technology, the commercialization of cultured meat is underway, with its inevitable integration into our diets on the horizon.

This Special Issue provides a platform for research and review papers, exploring recent advances in cultured meat science and technology. Contributions highlight core areas, including scaffold engineering, stem cell utilization, and novel approaches to cell proliferation and differentiation. The papers also include progress in cell culture media, antibiotic alternatives and FBS replacers. As the world pivots toward sustainable and ethical food production, this Special Issue serves as a comprehensive guide to the latest breakthroughs in cultured meat, offering crucial insights for researchers, industry professionals and policymakers navigating this transformative field.

Prof. Dr. Sung Gu Han
Guest Editor

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Keywords

  • cultured meat
  • in vitro meat
  • meat analog
  • cell culture technology
  • scaffold
  • sustainability
  • muscle satellite cell
  • stem cell

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Published Papers (3 papers)

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Research

14 pages, 3202 KiB  
Article
Influence of Gelatin on Adhesion, Proliferation, and Adipogenic Differentiation of Adipose Tissue-Derived Stem Cells Cultured on Soy Protein–Agarose Scaffolds
by Seong-Joon Hong, Do-Hyun Kim, Ji-Hwan Ryoo, Su-Min Park, Hyuk-Cheol Kwon, Dong-Hyun Keum, Dong-Min Shin and Sung-Gu Han
Foods 2024, 13(14), 2247; https://doi.org/10.3390/foods13142247 - 17 Jul 2024
Viewed by 847
Abstract
Scaffolds play a key role in cultured meat production by providing an optimal environment for efficient cell attachment, growth, and development. This study investigated the effects of gelatin coating on the adhesion, proliferation, and adipogenic differentiation of adipose tissue-derived stem cells (ADSCs) cultured [...] Read more.
Scaffolds play a key role in cultured meat production by providing an optimal environment for efficient cell attachment, growth, and development. This study investigated the effects of gelatin coating on the adhesion, proliferation, and adipogenic differentiation of adipose tissue-derived stem cells (ADSCs) cultured on soy protein–agarose scaffolds. Gelatin-coated scaffolds were prepared using 0.5% and 1.0% (w/v) gelatin solutions. The microstructure, water absorption rate, mechanical strength, cytotoxicity, cell adhesion, proliferation, and differentiation capabilities of the scaffolds were analyzed. Field emission scanning electron microscopy revealed the porous microstructure of the scaffolds, which was suitable for cell growth. Gelatin-coated scaffolds exhibited a significantly higher water absorption rate than that of non-coated scaffolds, indicating increased hydrophilicity. In addition, gelatin coating increased the mechanical strength of the scaffolds. Gelatin coating did not show cytotoxicity but significantly enhanced cell adhesion and proliferation. The gene expression levels of peroxisome proliferator-activated receptor gamma, CCAT/enhancer-binding protein alpha, and fatty acid-binding protein 4 were upregulated, and lipid accumulation was increased by gelatin coating. These findings suggest that gelatin-coated scaffolds provide a supportive microenvironment for ADSC growth and differentiation, highlighting their potential as a strategy for the improvement of cultured meat production and adipose tissue engineering. Full article
(This article belongs to the Special Issue Advances in Cultured Meat Science and Technology)
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16 pages, 5326 KiB  
Article
The Effects of Oleic Acid and Palmitic Acid on Porcine Muscle Satellite Cells
by Shah Ahmed Belal, Jeongeun Lee, Jinryong Park, Darae Kang and Kwanseob Shim
Foods 2024, 13(14), 2200; https://doi.org/10.3390/foods13142200 - 12 Jul 2024
Viewed by 799
Abstract
We aimed to determine the effects of oleic acid (OA) and palmitic acid (PA), alone or in combination, on proliferation, differentiation, triacylglycerol (TAG) content, and gene expression in porcine muscle satellite cells (PMSCs). Results revealed that OA-alone- and PA + OA-treated PMSCs showed [...] Read more.
We aimed to determine the effects of oleic acid (OA) and palmitic acid (PA), alone or in combination, on proliferation, differentiation, triacylglycerol (TAG) content, and gene expression in porcine muscle satellite cells (PMSCs). Results revealed that OA-alone- and PA + OA-treated PMSCs showed significantly increased viability than those in the control or PA-alone-treated groups. No significant effects on apoptosis were observed in all three treatments, whereas necrosis was significantly lower in OA-alone- and PA + OA-treated groups than in the control and PA-alone-treated groups. Myotube formation significantly increased in OA-alone and PA + OA-treated PMSCs than in the control and PA-alone-treated PMSCs. mRNA expression of the myogenesis-related genes MyoD1 and MyoG and of the adipogenesis-related genes PPARα, C/EBPα, PLIN1, FABP4, and FAS was significantly upregulated in OA-alone- and PA + OA-treated cells compared to control and PA-alone-treated cells, consistent with immunoblotting results for MyoD1 and MyoG. Supplementation of unsaturated fatty acid (OA) with/without saturated fatty acid (PA) significantly stimulated TAG accumulation in treated cells compared to the control and PA-alone-treated PMSCs. These results indicate that OA (alone and with PA) promotes proliferation by inhibiting necrosis and promoting myotube formation and TAG accumulation, likely upregulating myogenesis- and adipogenesis-related gene expression by modulating the effects of PA in PMSCs. Full article
(This article belongs to the Special Issue Advances in Cultured Meat Science and Technology)
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12 pages, 2448 KiB  
Article
Production of Plant-Based, Film-Type Scaffolds Using Alginate and Corn Starch for the Culture of Bovine Myoblasts
by Jun-Yeong Lee, Jihad Kamel, Chandra-Jit Yadav, Usha Yadav, Sadia Afrin, Yu-Mi Son, So-Yeon Won, Sung-Soo Han and Kyung-Mee Park
Foods 2024, 13(9), 1358; https://doi.org/10.3390/foods13091358 - 28 Apr 2024
Viewed by 1777
Abstract
Natural scaffolds have been the cornerstone of tissue engineering for decades, providing ideal environments for cell growth within extracellular matrices. Previous studies have favored animal-derived materials, including collagen, gelatin, and laminin, owing to their superior effects in promoting cell attachment, proliferation, and differentiation [...] Read more.
Natural scaffolds have been the cornerstone of tissue engineering for decades, providing ideal environments for cell growth within extracellular matrices. Previous studies have favored animal-derived materials, including collagen, gelatin, and laminin, owing to their superior effects in promoting cell attachment, proliferation, and differentiation compared to non-animal scaffolds, and used immortalized cell lines. However, for cultured meat production, non-animal-derived scaffolds with edible cells are preferred. Our study represents the first research to describe plant-derived, film-type scaffolds to overcome limitations associated with previously reported thick, gel-type scaffolds completely devoid of animal-derived materials. This approach has been employed to address the difficulties of fostering bovine muscle cell survival, migration, and differentiation in three-dimensional co-cultures. Primary bovine myoblasts from Bos Taurus Coreanae were harvested and seeded on alginate (Algi) or corn-derived alginate (AlgiC) scaffolds. Scaffold functionalities, including biocompatibility and the promotion of cell proliferation and differentiation, were evaluated using cell viability assays, immunofluorescence staining, and reverse transcription-quantitative polymerase chain reaction. Our results reveal a statistically significant 71.7% decrease in production time using film-type scaffolds relative to that for gel-type scaffolds, which can be maintained for up to 7 days. Film-type scaffolds enhanced initial cell attachment owing to their flatness and thinness relative to gel-type scaffolds. Algi and AlgiC film-type scaffolds both demonstrated low cytotoxicity over seven days of cell culture. Our findings indicated that PAX7 expression increased 16.5-fold in alginate scaffolds and 22.8-fold in AlgiC from day 1 to day 3. Moreover, at the differentiation stage on day 7, MHC expression was elevated 41.8-fold (Algi) and 32.7-fold (AlgiC), providing initial confirmation of the differentiation potential of bovine muscle cells. These findings suggest that both Algi and AlgiC film scaffolds are advantageous for cultured meat production. Full article
(This article belongs to the Special Issue Advances in Cultured Meat Science and Technology)
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