Search Results (31,648)

Human cytomegalovirus (HCMV) represents a highly medically important pathogen which has constantly been the subject of both molecular and clinical investigations. HCMV infections, especially those in high-risk patients, still raise many unanswered questions, so current investigations are focused on viral pathogenesis, vaccine development, and options for antiviral drug targeting. To this end, the use of suitable viral strains as well as recombinant reporter constructs in cultured cells and model systems has specific significance. We previously reported on the application of various herpesviruses that express green, red, or related fluorescent proteins, especially in the fields of virus–host interaction and antiviral research. Here, we characterized a recombinant version of the clinically relevant and cell type-adaptable HCMV strain TB40, which expresses firefly luciferase as a quantitative reporter of viral replication (TB40-FLuc). The data provide evidence for five main conclusions. First, HCMV TB40-FLuc is employable in multiple settings in primary human cells. Second, viral reporter signals are easily quantifiable, even at early time points within viral replication. Third, the FLuc reporter reflects the kinetics of viral intracellular replication, cascade-like viral IE-E-L protein production, and progeny release. Fourth, as relates to specific applications of the TB40-FLuc system, we demonstrated the reliability of quantitative antiviral compound determination in multi-well formats and its independence from fluorescence-based measurements in the case of autofluorescent inhibitors. Finally, we illustrated increased reporter sensitivity in comparison to other recombinant HCMVs. In essence, recombinant HCMV TB40-FLuc combines several molecular properties that are considered beneficial in studies on viral host tropism, replication efficiency, and antiviral drug assessment. Full article

Runoff simulation is essential for effective water resource management and plays a pivotal role in hydrological forecasting. Improving the quality of runoff simulation and forecasting continues to be a highly relevant research area. The complexity of the terrain and the scarcity of long-term runoff observation data have significantly limited the application of Physically Based Models (PBMs) in the Qinghai–Tibet Plateau (QTP). Recently, the Long Short-Term Memory (LSTM) network has been found to be effective in learning the dynamic hydrological characteristics of watersheds and outperforming some traditional PBMs in runoff simulation. However, the extent to which the LSTM works in data-scarce alpine regions remains unclear. This study aims to evaluate the applicability of LSTM in alpine basins in QTP, as well as the simulation performance of transfer-based LSTM (T-LSTM) in data-scarce alpine regions. The Lhasa River Basin (LRB) and Nyang River Basin (NRB) were the study areas, and the performance of the LSTM model was compared to that of PBMs by relying solely on the meteorological inputs. The results show that the average values of Nash–Sutcliffe efficiency (NSE), Kling–Gupta efficiency (KGE), and Relative Bias (RBias) for B-LSTM were 0.80, 0.85, and 4.21%, respectively, while the corresponding values for G-LSTM were 0.81, 0.84, and 3.19%. In comparison to a PBM- the Block-Wise use of TOPMEDEL (BTOP), LSTM has an average enhancement of 0.23, 0.36, and −18.36%, respectively. In both basins, LSTM significantly outperforms the BTOP model. Furthermore, the transfer learning-based LSTM model (T-LSTM) at the multi-watershed scale demonstrates that, when the input data are somewhat representative, even if the amount of data are limited, T-LSTM can obtain more accurate results than hydrological models specifically calibrated for individual watersheds. This result indicates that LSTM can effectively improve the runoff simulation performance in alpine regions and can be applied to runoff simulation in data-scarce regions. Full article

To meet the challenges of renewable energy consumption and improve the efficiency of energy systems, we propose an intelligent distributed energy dispatch strategy for multi-energy systems based on Nash bargaining by utilizing the power dispatch meta-universe platform. First, the operational framework of the multi-energy system, including wind park (WP), photovoltaic power plant (PVPP), and energy storage (ES), is described. Using the power dispatch meta-universe platform, the models of WP, PVPP, and ES are constructed and analyzed. Then, a Nash bargaining model of the multi-energy system is built and transformed into a coalition profit maximization problem, which is solved using the alternating direction multiplier method (ADMM). Finally, the effectiveness of the proposed strategy is verified. The results show that the strategy greatly improves the consumption of renewable energy sources and the profit of the overall system. Full article

The main objective of this work is to address problems related to the speed control of mobile robots with non-holonomic constraints and differential traction—specifically, robots for football games in the VSS (Very Small Size) category. To achieve this objective, an implementation and comparison is carried out between two control strategies: an adaptive control strategy by gain scheduling and a fuzzy multimodel intelligent control strategy. The mathematical models of the wheel motors for each operating range are approximated by a first-order system since data acquisition is performed using the step response. Tuning of the proportional and integral gains of the local controllers is carried out using the root locus technique in discrete time. For each mathematical model obtained for an operating range, a local controller is tuned. Finally, with the local controllers in hand, the implementation of and comparison between the gain scheduling adaptive control strategy and the fuzzy multimodel intelligent control strategy are carried out, in which the control strategies are programmed into the low-level code of a non-holonomic robot with a differential drive to verify the performance of the speed tracking dynamics imposed on the wheel motors to improve robot navigation during a robot football match. Full article

Identifying the catalytic regioselectivity of enzymes remains a challenge. Compared to experimental trial-and-error approaches, computational methods like molecular dynamics simulations provide valuable insights into enzyme characteristics. However, the massive data generated by these simulations hinder the extraction of knowledge about enzyme catalytic mechanisms without adequate modeling techniques. Here, we propose a computational framework utilizing graph-based active learning from molecular dynamics to identify the regioselectivity of ginsenoside hydrolases (GHs), which selectively catalyze C6 or C20 positions to obtain rare deglycosylated bioactive compounds from Panax plants. Experimental results reveal that the dynamic-aware graph model can excellently distinguish GH regioselectivity with accuracy as high as 96–98% even when different enzyme–substrate systems exhibit similar dynamic behaviors. The active learning strategy equips our model to work robustly while reducing the reliance on dynamic data, indicating its capacity to mine sufficient knowledge from short multi-replica simulations. Moreover, the model’s interpretability identified crucial residues and features associated with regioselectivity. Our findings contribute to the understanding of GH catalytic mechanisms and provide direct assistance for rational design to improve regioselectivity. We presented a general computational framework for modeling enzyme catalytic specificity from simulation data, paving the way for further integration of experimental and computational approaches in enzyme optimization and design. Full article

Recent advancements in mobile neural networks, such as the squeeze-and-excitation (SE) attention mechanism, have significantly improved model performance. However, they often overlook the crucial interaction between location information and channels. The interaction of multiple dimensions in feature engineering is of paramount importance for achieving high-quality results. The Transformer model and its successors, such as Mamba and Vision Mamba, have effectively combined features and linked location information. This approach has transitioned from NLP (natural language processing) to CV (computer vision). This paper introduces a novel attention mechanism for mobile neural networks inspired by the structure of Vim (Vision Mamba). It adopts a “1 + 3” architecture to embed multi-dimensional information into channel attention, termed ”Multi-Dimensional Vim-like Attention Mechanism”. The proposed method splits the input into two major branches: the left branch retains the original information for subsequent feature screening, while the right branch divides the channel attention into three one-dimensional feature encoding processes. These processes aggregate features along one channel direction and two spatial directions, simultaneously capturing remote dependencies and preserving precise location information. The resulting feature maps are then combined with the left branch to produce direction-aware, location-sensitive, and channel-aware attention maps. The multi-dimensional Vim-like attention module is simple and can be seamlessly integrated into classical mobile neural networks such as MobileNetV2 and ShuffleNetV2 with minimal computational overhead. Experimental results demonstrate that this attention module adapts well to mobile neural networks with a low parameter count, delivering excellent performance on the CIFAR-100 and MS COCO datasets. Full article

The use of industrial robots for machining operations is pursued by industry lately, since they can increase the flexibility of the production system and reduce production costs. However, their industrial adoption is still limited, mainly due to their insufficient structural stiffness and posture-dependent dynamic behavior, leading to limited machining process accuracy. For this purpose, the Digital-Model of a machining robot has been developed, providing a tool for virtual commissioning of the process that can be used during the process planning stage. The Multi-Body Simulation method combined with a Component Mode Synthesis have been adopted, considering flexibility of both the joints and links. On top of that, and motivated from robotic-based machining systems’ flexibility and versatility, two optimization algorithms have been developed, attempting to increase the process accuracy. A workpiece placement optimization algorithm, attempting to maximize the robot stiffness during the process acquiring knowledge from the robot stiffness maps, and a feed-rate scheduling algorithm, attempting to constrain the contour error by regulating the generated cutting forces. The capabilities and functionality of the developed model and optimization algorithms are showcased in two different case studies, with the results proving the improvements on the process accuracy after the application of the optimization algorithms. Finally, an experimental validation of the Digital-Model has been performed, to confirm the consistency between model outputs and real experimental data. Full article

As semiconductor integration scales expand and chip sizes shrink, Through Silicon Via (TSV) technology advances towards smaller diameters and higher aspect ratios, posing significant challenges in thermo-mechanical reliability, particularly within interposer substrates where mismatched coefficients of thermal expansion exacerbate issues. This study conducts a thermo-mechanical analysis of TSV structures within multi-layered complex interposers, and analyzes the thermal stress behavior and reliability under variable temperature conditions (−55 °C to 85 °C), taking into account the typical electroplating defects within the copper pillars in TSVs. Initially, an overall model is established to determine the critical TSV locations. Sub-model analysis is then employed to investigate the stress and deformation of the most critical TSV, enabling the calculation of the temperature cycle life accordingly. Results indicate that the most critical TSV resides centrally within the model, exhibiting the highest equivalent stress. During the temperature cycling process, the maximum deformation experiences approximately periodic variations, while the maximum equivalent stress undergoes continuous accumulation and gradually diminishes. Its peak occurs at the contact interface corner between the TSV and Redistribution Layer (RDL). The estimated life of the critical point is 3.1708 × 105 cycles. Furthermore, it is observed that electroplating defect b alleviates thermal stress within TSVs during temperature cycling. This study provides insights into TSV thermal behavior and reliability, which are crucial for optimizing the design and manufacturing processes of advanced semiconductor packaging. Full article

Aim of the Study: To assess how virtual reality (VR) patient-specific simulations can support decision-making processes and improve care in pediatric urology, ultimately improving patient outcomes. Patients and Methods: Children diagnosed with urological conditions necessitating complex procedures were retrospectively reviewed and enrolled in the study. Patient-specific VR simulations were developed with medical imaging specialists and VR technology experts. Routine CT images were utilized to create a VR environment using advanced software platforms. The accuracy and fidelity of the VR simulations was validated through a multi-step process. This involved comparing the virtual anatomical models to the original medical imaging data and conducting feedback sessions with pediatric urology experts to assess VR simulations’ realism and clinical relevance. Results: A total of six pediatric patients were reviewed. The median age of the participants was 5.5 years (IQR: 3.5–8.5 years), with an equal distribution of males and females across both groups. A minimally invasive laparoscopic approach was performed for adrenal lesions (n = 3), Wilms’ tumor (n = 1), bilateral nephroblastomatosis (n = 1), and abdominal trauma in complex vascular and renal malformation (ptotic and hypoplastic kidney) (n = 1). Key benefits included enhanced visualization of the segmental arteries and the deep vascularization of the kidney and adrenal glands in all cases. The high depth perception and precision in the orientation of the arteries and veins to the parenchyma changed the intraoperative decision-making process in five patients. Preoperative VR patient-specific simulation did not offer accuracy in studying the pelvic and calyceal anatomy. Conclusions: VR patient-specific simulations represent an empowering tool in pediatric urology. By leveraging the immersive capabilities of VR technology, preoperative planning and intraoperative navigation can greatly impact surgical decision-making. As we continue to advance in medical simulation, VR holds promise in educational programs to include even surgical treatment of more complex urogenital malformations. Full article

In the context of power-line scenarios characterized by complex backgrounds and diverse scales and shapes of targets, and addressing issues such as large model parameter sizes, insufficient feature extraction, and the susceptibility to missing small targets in engineering-vehicle detection tasks, a lightweight detection algorithm termed CER-YOLOv5s is firstly proposed. The C3 module was restructured by embedding a lightweight Ghost bottleneck structure and convolutional attention module, enhancing the model’s ability to extract key features while reducing computational costs. Secondly, an E-BiFPN feature pyramid network is proposed, utilizing channel attention mechanisms to effectively suppress background noise and enhance the model’s focus on important regions. Bidirectional connections were introduced to optimize the feature fusion paths, improving the efficiency of multi-scale feature fusion. At the same time, in the feature fusion part, an ERM (enhanced receptive module) was added to expand the receptive field of shallow feature maps through multiple convolution repetitions, enhancing the global information perception capability in relation to small targets. Lastly, a Soft-DIoU-NMS suppression algorithm is proposed to improve the candidate box selection mechanism, addressing the issue of suboptimal detection of occluded targets. The experimental results indicated that compared with the baseline YOLOv5s algorithm, the improved algorithm reduced parameters and computations by 27.8% and 31.9%, respectively. The mean average precision (mAP) increased by 2.9%, reaching 98.3%. This improvement surpasses recent mainstream algorithms and suggests stronger robustness across various scenarios. The algorithm meets the lightweight requirements for embedded devices in power-line scenarios. Full article

In this paper, a network comprising wireless devices equipped with buffers transmitting deadline-constrained data packets over a slotted-ALOHA random-access channel is studied. Although communication protocols facilitating retransmissions increase reliability, a packet awaiting transmission from the queue experiences delays. Thus, packets with time constraints might be dropped before being successfully transmitted, while at the same time causing the queue size of the buffer to increase. To understand the trade-off between reliability and delays that might lead to packet drops due to deadline-constrained bursty traffic with retransmissions, the scenario of a wireless network utilizing a slotted-ALOHA random-access channel is investigated. The main focus is to reveal the trade-off between the number of retransmissions and the packet deadline as a function of the arrival rate. Towards this end, analysis of the system is performed by means of discrete-time Markov chains. Two scenarios are studied: (i) the collision channel model (in which a receiver can decode only when a single packet is transmitted), and (ii) the case for which receivers have multi-packet reception capabilities. A performance evaluation for a user with different transmit probabilities and number of retransmissions is conducted. We are able to determine numerically the optimal probability of transmissions and the number of retransmissions, given the packet arrival rate and the packet deadline. Furthermore, we highlight the impact of transmit probability and the number of retransmissions on the average drop rate and throughput. Full article

Insulated gate bipolar transistors (IGBTs) are widely used in power electronic devices, and their health prediction problems have attracted much attention in the field of power electronic equipment health management. The performance degradation of IGBT gate oxide is one of the most important failure modes. In order to analyze this failure mechanism and the ease of implementation of a monitoring circuit, the gate leakage current of IGBTs was selected as the fault precursor parameter for the degradation of their gate oxide performance, and feature selection and fusion were carried out by using time domain characteristic analysis, grayscale correlation, Mahalanobis distance, Kalman filter, and other methods. Thus, a health indicator was obtained to characterize the degradation of IGBT performance, which was used to indicate the degree of aging of the IGBT gate oxide layer. In this paper, we propose an improved degradation prediction model called MultiScaleFormer, inspired by advanced design ideas of the iTransformer network architecture, combined with the health parameters of IGBTs to construct a degradation prediction model for the IGBT gate oxide layer. MultiScaleFormer showed the highest fitting accuracy compared with the Long Short-Term Memory (LSTM), Convolutional Neural Network (CNN), Support Vector Regression (SVR), Gaussian Process Regression (GPR), CNN-LSTM, and Transformer models in our experiment. The mean absolute error (MAE) of the MultiScaleFormer prediction was as low as 0.0087. Extraction of the health indicator and the construction and verification of the degradation prediction model were carried out on the dataset released by the NASA-Ames Laboratory. These results demonstrate the feasibility of the gate leakage current as a fault precursor parameter for IGBT gate oxide failure, and the feasibility and accuracy of the MultiScaleFormer prediction model for IGBT performance degradation. Full article

Circular synthetic aperture radar (CSAR) has attracted a lot of interest, recently, for its excellent performance in civilian and military applications. However, in CSAR imaging, the result is to be defocused when the height of an object deviates from a reference height. Existing approaches for this problem rely on digital elevation models (DEMs) for error compensation. It is difficult and costly to collect DEM using specific equipment, while the inversion of DEM based on echo is computationally intensive, and the accuracy of results is unsatisfactory. Inspired by multi-focus image fusion in optical images, a spatial-domain fusion method is proposed based on the sum of modified Laplacian (SML) and guided filter. After obtaining CSAR images in a stack of different reference heights, an all-in-focus image can be computed by the proposed method. First, the SMLs of all source images are calculated. Second, take the rule of selecting the maximum value of SML pixel by pixel to acquire initial decision maps. Secondly, a guided filter is utilized to correct the initial decision maps. Finally, fuse the source images and decision maps to obtain the result. A comparative experiment has been processed to verify the exceptional performance of the proposed method. The final processing result of real-measured CSAR data demonstrated that the proposed method is effective and practical. Full article

Accurate long-term PM_2.5 prediction is crucial for environmental management and public health. However, previous studies have mainly focused on short-term air quality point predictions, neglecting the importance of accurately predicting the long-term trends of PM_2.5 and studying the uncertainty of PM_2.5 concentration changes. The traditional approaches have limitations in capturing nonlinear relationships and complex dynamic patterns in time series, and they often overlook the credibility of prediction results in practical applications. Therefore, there is still much room for improvement in long-term prediction of PM_2.5. This study proposes a novel long-term point and interval prediction framework for urban air quality based on multi-source spatial and temporal data, which further quantifies the uncertainty and volatility of the prediction based on the accurate PM_2.5 point prediction. In this model, firstly, multi-source datasets from multiple monitoring stations are preprocessed. Subsequently, spatial clustering of stations based on POI data is performed to filter out strongly correlated stations, and feature selection is performed to eliminate redundant features. In this paper, the ConvFormer-KDE model is presented, whereby local patterns and short-term dependencies among multivariate variables are mined through a convolutional neural network (CNN), long-term dependencies among time-series data are extracted using the Transformer model, and a direct multi-output strategy is employed to realize the long-term point prediction of PM_2.5 concentration. KDE is utilized to derive prediction intervals for PM_2.5 concentration at confidence levels of 85%, 90%, and 95%, respectively, reflecting the uncertainty inherent in long-term trends of PM_2.5. The performance of ConvFormer-KDE was compared with a list of advanced models. Experimental results showed that ConvFormer-KDE outperformed baseline models in long-term point- and interval-prediction tasks for PM_2.5. The ConvFormer-KDE can provide a valuable early warning basis for future PM_2.5 changes from the aspects of point and interval prediction. Full article

The objective was to compare the color match and color stability behavior of single- and multi-shade resin-based composites (RBCs) used for direct restorations. This review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses guidelines. Randomized clinical trials evaluating the shade performance of single-shade RBCs in direct restorations were included. A search of the scientific literature was performed in five databases (April 2024). The meta-analysis was performed using RevMan 5.4, calculating the risk difference (RD) and 95% confidence interval (CI) of the dichotomous outcome using a random effects model. Bias was assessed using the RoB 2.0 tool, and certainty of evidence was assessed using the GRADEpro tool. Four studies were selected, with 263 restorations analyzed. The results showed comparable performance between single-shade RBCs and multi-shade RBCs in terms of color match and color stability over 12 months. Three studies had a low risk of bias with all expected results, and one study had some concerns. The certainty of evidence for color stability was considered low for all follow-up periods due to the small number of events and sample size. According to the United States Public Health Service Evaluation (USPHS) and the World Dental Federation (FDI), there is comparable clinical color performance between single-shade and multi-shade RBCs over 12 months. Full article