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Keywords = ecological setting

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17 pages, 2302 KiB  
Article
Modulation of Growth and Mycotoxigenic Potential of Pineapple Fruitlet Core Rot Pathogens during In Vitro Interactions
by Manon Vignassa, Christian Soria, Noël Durand, Charlie Poss, Jean-Christophe Meile, Marc Chillet and Sabine Schorr-Galindo
Toxins 2024, 16(8), 344; https://doi.org/10.3390/toxins16080344 - 7 Aug 2024
Abstract
Pineapple Fruitlet Core Rot (FCR) is a fungal disease characterized by a multi-pathogen pathosystem. Recently, Fusarium proliferatum, Fusarium oxysporum, and Talaromyces stollii joined the set of FCR pathogens until then exclusively attributed to Fusarium ananatum. The particularity of FCR relies on the presence of [...] Read more.
Pineapple Fruitlet Core Rot (FCR) is a fungal disease characterized by a multi-pathogen pathosystem. Recently, Fusarium proliferatum, Fusarium oxysporum, and Talaromyces stollii joined the set of FCR pathogens until then exclusively attributed to Fusarium ananatum. The particularity of FCR relies on the presence of healthy and diseased fruitlets within the same infructescence. The mycobiomes associated with these two types of tissues suggested that disease occurrence might be triggered by or linked to an ecological chemical communication-promoting pathogen(s) development within the fungal community. Interactions between the four recently identified pathogens were deciphered by in vitro pairwise co-culture bioassays. Both fungal growth and mycotoxin production patterns were monitored for 10 days. Results evidenced that Talaromyces stollii was the main fungal antagonist of Fusarium species, reducing by 22% the growth of Fusarium proliferatum. A collapse of beauvericin content was observed when FCR pathogens were cross-challenged while fumonisin concentrations were increased by up to 7-fold. Antagonism between Fusarium species and Talaromyces stollii was supported by the diffusion of a red pigmentation and droplets of red exudate at the mycelium surface. This study revealed that secondary metabolites could shape the fungal pathogenic community of a pineapple fruitlet and contribute to virulence promoting FCR establishment. Full article
(This article belongs to the Special Issue Toxins: 15th Anniversary)
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36 pages, 3292 KiB  
Article
Energy and Carbon Savings in European Households Resulting from Behavioral Changes
by Barbara Widera
Energies 2024, 17(16), 3888; https://doi.org/10.3390/en17163888 - 7 Aug 2024
Viewed by 197
Abstract
The study evaluates the impact of behavioral changes resulting from climate awareness on energy consumption and carbon emissions in European households based on the results of a two-stage survey addressed to individuals manifesting pro-ecological attitudes. In the first stage, the author analyzed 67 [...] Read more.
The study evaluates the impact of behavioral changes resulting from climate awareness on energy consumption and carbon emissions in European households based on the results of a two-stage survey addressed to individuals manifesting pro-ecological attitudes. In the first stage, the author analyzed 67 pro-environmental behaviors declared by the participants, identified a set of new sustainable choices, and compared them to the conservation habits used in Rasch and Campbell’s models. The 10 most popular initiatives undertaken by over 50% of participants were selected for further analysis. The influence of these initiatives on energy consumption and CO2 emissions was assessed. A total of 24 impact indicators were identified at the building scale. Energy and carbon savings were calculated for 500 participants from 28 European countries and compared to the results computed for the 100 households used as a reference. The main conclusions from the research concern the significance of individual decisions at the building scale in the context of their actual environmental impact calculated for a larger scale. The comparative analysis showed that the highest annual energy (2292.1 MWh) and emission (267.02 tons of CO2) savings resulted from the car-to-bicycle (or walking) transition on short-distance trips (declared by 79%) and from the transition from non-renewable to renewable energy sources (PV panels installed by 65% of respondents). Annual energy and emission savings reached, respectively, 1300 MWh and 262.6 tons of CO2. The research findings help explain the critical importance of transforming the built environment towards renewable energy sources and supporting pedestrian and sustainable transportation. Full article
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7 pages, 1256 KiB  
Brief Report
A Synthetic Framework to Match Concepts and Approaches When Managing Anthropogenic Threats
by Corrado Battisti, Anna Testi, Giuliano Fanelli, Milvia Rastrelli, Pietro Giovacchini and Letizia Marsili
Conservation 2024, 4(3), 395-401; https://doi.org/10.3390/conservation4030026 - 5 Aug 2024
Viewed by 297
Abstract
Anthropogenic threats impacting ecological targets should be mitigated and solved using fast and schematic tools useful in conservation strategies. Herein, we suggest a mixed and quick approach implementing coarse-grained (and expert-based) threat analysis with the fine-grained (and analytical) DPSIR (driving forces, pressure, status, [...] Read more.
Anthropogenic threats impacting ecological targets should be mitigated and solved using fast and schematic tools useful in conservation strategies. Herein, we suggest a mixed and quick approach implementing coarse-grained (and expert-based) threat analysis with the fine-grained (and analytical) DPSIR (driving forces, pressure, status, impact, and response) framework of indicators, all included in a single causal chain. Both approaches are largely used in conservation but never combined. A simulated example of the application of the set of indicators (status, pressure, impact, and response) on dune ecosystems (and nested targets represented by halo-psammophilous plants) has been included. Due to its schematic format, values as targets, pressures as threats, and responses as conservation strategies have been unified in a single conceptual framework. This synthetic framework can also be used to communicate to academic students the complexities of socio-ecological systems on the conservation front lines using a simplified cause–effect chain. Full article
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17 pages, 26856 KiB  
Article
Changed Seasonality and Forcings of Peak Annual Flows in Ephemeral Channels at Flagstaff, Northern Arizona, USA
by Erik Schiefer and Edward Schenk
Hydrology 2024, 11(8), 115; https://doi.org/10.3390/hydrology11080115 - 3 Aug 2024
Viewed by 246
Abstract
Flood variability associated with urbanization, ecological change, and climatic change is of increasing economic and social concern in and around Flagstaff, Arizona, where flood hydrology is influenced by a biannual precipitation regime and the relatively unique geologic setting at the edge of the [...] Read more.
Flood variability associated with urbanization, ecological change, and climatic change is of increasing economic and social concern in and around Flagstaff, Arizona, where flood hydrology is influenced by a biannual precipitation regime and the relatively unique geologic setting at the edge of the San Francisco Volcanic Field on the southern edge of the Colorado Plateau. There has been limited long-term gauging of the ephemeral channels draining the developed lands and dry coniferous forests of the region, resulting in a spaciotemporal gap in observation-based assessments of large-scale flooding patterns. We present new data from over 10 years of flood monitoring using a crest stage gauge network, combined with other channel monitoring records from multiple agency sources, to assess inter-decadal patterns of flood change in the area, with a specific emphasis on examining how various controls and disturbances have altered the character and seasonality of peak annual flows. Methods of analysis included the following: using Fisher’s Exact Test to compare the seasonality of flooding between historic data spanning the 1970s and contemporary data obtained since 2010; summarizing GIS-based spatial data and meteorological timeseries to characterize study catchment conditions and changes between flood study periods; and relating spatiotemporal patterns of flood seasonality and occurrences of notably large floods with catchment characteristics and environmental changes. Our results show systematic patterns and changes in Flagstaff-area flood regimes that relate to geologic and topographic controls of the varied catchment systems, and in response to records of climate variations and local catchment disturbances, including urbanization and, especially, high-severity wildfire. For most catchments there has been a shift from predominantly late winter to spring snowmelt floods, or mixed seasonal flood regimes, towards monsoon-dominated flooding, patterns which may relate to observed local warming and precipitation changes. Post-wildfire flooding has produced extreme flood discharges which have likely exceeded historical estimates of flood magnitude over decade-long monitoring periods by one to two orders of magnitude. We advocate for continued monitoring and the expansion of local stream gauge networks to enable seasonal, magnitude-frequency trend analyses, improved climate and environmental change attribution, and to better inform the many planned and ongoing flood mitigation projects being undertaken in the increasingly developed Flagstaff region. Full article
(This article belongs to the Special Issue Runoff Modelling under Climate Change)
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16 pages, 1299 KiB  
Article
Sensory Analysis Performed within Augmented Virtuality System: Impact on Hedonic Scores, Engagement, and Presence Level
by José Carlos Ribeiro, Célia Rocha, Bruna Barbosa, Rui Costa Lima and Luís Miguel Cunha
Foods 2024, 13(15), 2456; https://doi.org/10.3390/foods13152456 - 3 Aug 2024
Viewed by 424
Abstract
Sensory analysis methodologies are performed in sensory booths designed to minimise external stimuli, lacking ecological validity. Immersive environments are used to introduce contextual cues, but there is a lack of studies using mixed reality systems. The main goal of this study was to [...] Read more.
Sensory analysis methodologies are performed in sensory booths designed to minimise external stimuli, lacking ecological validity. Immersive environments are used to introduce contextual cues, but there is a lack of studies using mixed reality systems. The main goal of this study was to evaluate an augmented virtuality (AV) system where participants are inserted into a virtual environment and evaluate a real product, being able to interact with both dimensions. A panel of 102 consumers evaluated five samples of commercial peach nectars in three sessions, each in a different environment: public food court, living room (AV environments), and laboratory (traditional sensory booth). Consumers rated overall liking, followed by open comments, and also answered an Engagement (EQ) and a Presence Questionnaire (PQ). The type of environment only affected hedonic discrimination among samples, with the laboratory setting being the only one with sample discrimination. Nonetheless, each sample was not evaluated differently across the different environments. Concerning engagement, the environment only significantly influenced the EQ’s ‘Affective Value’ factor, being higher when using an AV system. The level of presence in the virtual environment was significantly higher in the public food court, being significantly correlated with the EQ factor scores. Full article
(This article belongs to the Special Issue Sensory and Consumer Science in the Green Transition)
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22 pages, 9734 KiB  
Article
Implications of Water Quality Index and Multivariate Statistics for Improved Environmental Regulation in the Irtysh River Basin (Kazakhstan)
by Ultuar Zhalmagambetova, Daulet Assanov, Alexandr Neftissov, Andrii Biloshchytskyi and Ivan Radelyuk
Water 2024, 16(15), 2203; https://doi.org/10.3390/w16152203 - 2 Aug 2024
Viewed by 494
Abstract
The selection of sites for permanent environmental monitoring of natural water bodies should rely on corresponding source apportionment studies. Tools like the water quality index (WQI) assessment may support this objective. This study aims to analyze a decade-long dataset of measurements of 26 [...] Read more.
The selection of sites for permanent environmental monitoring of natural water bodies should rely on corresponding source apportionment studies. Tools like the water quality index (WQI) assessment may support this objective. This study aims to analyze a decade-long dataset of measurements of 26 chemical components at 26 observation points within the Irtysh River Basin, aiming to identify priority zones for stricter environmental regulations. It was achieved through the WQI tool integrated with geoinformation systems (GISs) and multivariate statistical techniques. The findings highlighted that both upstream sections of tributaries (Oba and Bukhtarma rivers) and the mainstream of the basin are generally in good condition, with slight fluctuations observed during flooding periods. Areas in the basin experiencing significant impacts from mining and domestic wastewater treatment activities were identified. The rivers Glubochanka (GL) and Krasnoyarka (KR) consistently experienced marginal water quality throughout the observation period. Various contaminant sources were found to influence water quality. The impact of domestic wastewater treatment facilities was represented by twofold elevated concentrations of chemical oxygen demand, reaching 22.6 and 27.1 mg/L for the KR and GL rivers, respectively. Natural factors were indicated by consistent slight exceedings of recommended calcium levels at the KR and GL rivers. These exceedances were most pronounced during the cold seasons, with an average value equal to 96 mg/L. Mining operations introduced extremal concentrations of trace elements like copper, reaching 0.046–0.051 mg/L, which is higher than the threshold by 12–13 times; zinc, which peaked at 1.57–2.96 mg/L, exceeding the set limit by almost 50–100 times; and cadmium, peaking at levels surpassing 1000 times the safe limit, reaching 0.8 mg/L. The adverse impact of mining activities was evident in the Tikhaya, Ulba, and Breksa rivers, showing similar trends in trace element concentrations. Seasonal effects were also investigated. Ice cover formation during cold seasons led to oxygen depletion and the exclusion of pollutants into the stream when ice melted, worsening water quality. Conversely, flooding events led to contaminant dilution, partially improving the WQI during flood seasons. Principal component analysis and hierarchical cluster analysis indicated that local natural processes, mining activities, and domestic wastewater discharge were the predominant influences on water quality within the study area. These findings can serve as a basis for enhanced environmental regulation in light of updated ecological legislation in Kazakhstan, advocating for the establishment of a comprehensive monitoring network and the reinforcement of requirements governing contaminating activities. Full article
(This article belongs to the Section Water Quality and Contamination)
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12 pages, 6293 KiB  
Article
Econormative Childhoods in Wimmelbooks on the Four Seasons: Analysis of Central European Wordless Informational Picturebooks
by Krzysztof Rybak
Literature 2024, 4(3), 172-183; https://doi.org/10.3390/literature4030013 - 31 Jul 2024
Viewed by 296
Abstract
More and more informational picturebooks on environmental topics have been published in recent years, many focusing on the inevitable climate change. Conversely, there is still a tendency in contemporary picturebooks to represent the climate traditionally, irrespective of actual climate change. A particularly interesting [...] Read more.
More and more informational picturebooks on environmental topics have been published in recent years, many focusing on the inevitable climate change. Conversely, there is still a tendency in contemporary picturebooks to represent the climate traditionally, irrespective of actual climate change. A particularly interesting case is the representation of the seasons, especially in books aimed at the youngest ‘readers’, such as wimmelbooks. Not only are they crucial for developing emergent and visual literacy, but they also contain normative images that constitute a prototype for the child. The ‘norms’ picturebooks present are based on the authors’ ideologies that constitute all informational picturebooks as their authors interpret facts. Hence, this article aims to analyse the visual strategies used and the ideologies expressed by wimmelbooks from Poland and Germany in representing the seasons (Marcin Strzembosz’s Jaki to miesiąc? [2002] and Ali Mitgutsch’s Mein Wimmel-Bilderbuch: Frühling, Sommer, Herbst und Winter [2007], among others). The preliminary research shows that the authors seem to propose traditional, idyllic, ecologically normative images of the environment, which I propose to call econormative (inspired by the word ‘aetonormative’), such as snowy winters, sunny summers, etc.; hence, wimmelbooks seem to assent to stereotypical depictions of the seasons associated with the notion of ideal childhoods set in econormative environments. Full article
(This article belongs to the Special Issue Constructions of Childhood(s) in Fiction and Nonfiction for Children)
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14 pages, 1976 KiB  
Article
Overcoming Barriers and Fostering Adoption: Evaluating the Institutional Mainstreaming of Nature-Based Solutions in the Emilia-Romagna Region’s Socio-Ecological System
by Teresa Carlone and Matteo Mannocchi
Land 2024, 13(8), 1175; https://doi.org/10.3390/land13081175 - 30 Jul 2024
Viewed by 280
Abstract
International organizations like the EU and IUCN are advocating for nature-based solutions (NBSs) as green alternatives for climate change adaptation and mitigation, especially in disaster risk reduction and urban planning. The H2020 OPERANDUM project was designed to address the major hydro-meteorological risks (floods, [...] Read more.
International organizations like the EU and IUCN are advocating for nature-based solutions (NBSs) as green alternatives for climate change adaptation and mitigation, especially in disaster risk reduction and urban planning. The H2020 OPERANDUM project was designed to address the major hydro-meteorological risks (floods, droughts, landslides, storm surge, and coastal erosions) through the deployment and assessment of NBSs in different contexts and areas affected by specific hazards. Despite growing research and funding, NBSs are still in the early stages of mainstream adoption and face challenges in acceptance and dissemination. Although designed to benefit both social and ecological systems, they remain a niche area with low perceived effectiveness among technicians and decision-makers. Their uptake requires a paradigm shift that includes a change in cultural-cognitive institutions, a different and wider set of knowledge than traditional engineering (ecological, social), and an adaptive management approach, missing within the current governance system. Using a qualitative case study research method, this paper aims to identify barriers in mainstreaming NBSs for DRR (disaster risk reduction) in the Emilia-Romagna region—influenced not only by individual beliefs but also by variables tied to technical culture and local procedural norms—and emphasizing the importance of combining social and ecological indicators in socio-ecological system analysis. Full article
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22 pages, 22301 KiB  
Article
Projecting the Impacts of Climate Change, Soil, and Landscape on the Geographic Distribution of Ma Bamboo (Dendrocalamus latiflorus Munro) in China
by Li-Jia Chen, Yan-Qiu Xie, Tian-You He, Ling-Yan Chen, Jun-Dong Rong, Li-Guang Chen and Yu-Shan Zheng
Forests 2024, 15(8), 1321; https://doi.org/10.3390/f15081321 - 29 Jul 2024
Viewed by 356
Abstract
Ma bamboo (Dendrocalamus latiflorus Munro) is a fast-growing woody grass that offers significant economic benefits, including materials for construction, furniture, biofuel, food, and handicrafts. It also provides ecological benefits like soil conservation, wildlife habitats, and carbon sequestration. However, its species distribution patterns [...] Read more.
Ma bamboo (Dendrocalamus latiflorus Munro) is a fast-growing woody grass that offers significant economic benefits, including materials for construction, furniture, biofuel, food, and handicrafts. It also provides ecological benefits like soil conservation, wildlife habitats, and carbon sequestration. However, its species distribution patterns are influenced by various factors, including climate (mainly temperature and precipitation), soil attributes, and landscape characteristics such as topography, land use, and vegetation. Understanding these impacts is essential for the sustainable management of D. latiflorus resources and fostering related economic activities. To address these challenges, we developed a comprehensive habitat suitability (CHS) model that integrates climate, soil, and landscape variables to simulate the distribution dynamics of D. latiflorus under different shared socio-economic pathway (SSP) scenarios. An ensemble model (EM) strategy was applied to each variable set to ensure robust predictions. The results show that the current potential distribution of D. latiflorus spans 28.95 × 104 km2, primarily located in South China and the Sichuan Basin. Its distribution is most influenced by the annual mean temperature (Bio1), the cation exchange capacity of soil clay particles in the 20–40 cm soil layer (CECc 20–40 cm), vegetation, and elevation. Under future climate scenarios, these habitats are projected to initially expand slightly and then contract, with a northward shift in latitude and migration to higher elevations. Additionally, the Sichuan Basin (Sichuan–Chongqing border) is identified as a climatically stable area suitable for germplasm development and conservation. To conclude, our findings shed light on how climate change impacts the geographic distribution of D. latiflorus, providing key theoretical foundations for its sustainable cultivation and conservation strategies. Full article
(This article belongs to the Section Forest Ecology and Management)
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14 pages, 1854 KiB  
Article
Mortality Attributable to Clostridioides difficile Infection: The Rising Burden of Disease in European Countries
by Irena Ilic, Ivana Zivanovic Macuzic and Milena Ilic
Medicina 2024, 60(8), 1222; https://doi.org/10.3390/medicina60081222 - 28 Jul 2024
Viewed by 316
Abstract
Background and Objectives: Clostridioides difficile infection is a major public health issue, being among the main causes of mortality due to healthcare-associated diarrhea. This study aimed to assess the trends in mortality attributable to Clostridioides difficile infections in European countries over a [...] Read more.
Background and Objectives: Clostridioides difficile infection is a major public health issue, being among the main causes of mortality due to healthcare-associated diarrhea. This study aimed to assess the trends in mortality attributable to Clostridioides difficile infections in European countries over a period of 30 years. Materials and Methods: A descriptive epidemiological study was conducted, with the application of an ecological study design, to evaluate the trends in mortality due to Clostridioides difficile infection in the Central, Eastern, and Western European sub-regions from 1990 to 2019. The Global Burden of Disease study database was used. Trends were evaluated with the joinpoint regression analysis. Results: In both sexes, about 76% of all deaths attributable to Clostridioides difficile infections were recorded in the Western European sub-region in 2019. The age-standardized rates of the burden of Clostridioides difficile infection in 2019 were the highest in the Central European sub-region, followed by the Western European sub-region, while the lowest rates were observed in the Eastern European sub-region. A significantly increasing trend in mortality attributable to Clostridioides difficile infection from 1990 to 2019 was recorded both in males (by +2.1% per year) and females (by +2.8% per year). The burden of Clostridioides difficile infection showed increasing trends in most of the European countries, significantly correlating with the country’s development, according to the Human Development Index. Conclusions: The rising burden of Clostridioides difficile infection in European countries in the last few decades suggests a need for improving public health measures, with a focus both on the hospital setting and community. Full article
(This article belongs to the Section Infectious Disease)
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14 pages, 3158 KiB  
Article
Unobtrusive Skin Temperature Estimation on a Smart Bed
by Gary Garcia-Molina, Trevor Winger, Nikhil Makaram, Megha Rajam Rao, Pavlo Chernega, Yehor Shcherbakov, Leah McGhee, Vidhya Chellamuthu, Erwin Veneros, Raj Mills, Mark Aloia and Kathryn J. Reid
Sensors 2024, 24(15), 4882; https://doi.org/10.3390/s24154882 - 27 Jul 2024
Viewed by 360
Abstract
The transition from wakefulness to sleep occurs when the core body temperature decreases. The latter is facilitated by an increase in the cutaneous blood flow, which dissipates internal heat into the micro-environment surrounding the sleeper’s body. The rise in cutaneous blood flow near [...] Read more.
The transition from wakefulness to sleep occurs when the core body temperature decreases. The latter is facilitated by an increase in the cutaneous blood flow, which dissipates internal heat into the micro-environment surrounding the sleeper’s body. The rise in cutaneous blood flow near sleep onset causes the distal (hands and feet) and proximal (abdomen) temperatures to increase by about 1 °C and 0.5 °C, respectively. Characterizing the dynamics of skin temperature changes throughout sleep phases and understanding its relationship with sleep quality requires a means to unobtrusively and longitudinally estimate the skin temperature. Leveraging the data from a temperature sensor strip (TSS) with five individual temperature sensors embedded near the surface of a smart bed’s mattress, we have developed an algorithm to estimate the distal skin temperature with a minute-long temporal resolution. The data from 18 participants who recorded TSS and ground-truth temperature data from sleep during 14 nights at home and 2 nights in a lab were used to develop an algorithm that uses a two-stage regression model (gradient boosted tree followed by a random forest) to estimate the distal skin temperature. A five-fold cross-validation procedure was applied to train and validate the model such that the data from a participant could only be either in the training or validation set but not in both. The algorithm verification was performed with the in-lab data. The algorithm presented in this research can estimate the distal skin temperature at a minute-level resolution, with accuracy characterized by the mean limits of agreement [−0.79 to +0.79 °C] and mean coefficient of determination R2=0.87. This method may enable the unobtrusive, longitudinal and ecologically valid collection of distal skin temperature values during sleep. Therelatively small sample size motivates the need for further validation efforts. Full article
(This article belongs to the Section Biomedical Sensors)
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25 pages, 7464 KiB  
Systematic Review
Research Geographical Distribution, Strategies, and Environmental and Socioeconomic Factors Influencing the Success of Land-Based Restoration: A Systematic Review
by Jonathan O. Hernandez, Damcelle T. Cortes and Byung Bae Park
Sustainability 2024, 16(15), 6381; https://doi.org/10.3390/su16156381 - 25 Jul 2024
Viewed by 408
Abstract
The effectiveness of restoration programs may differ in terms of environmental and socioeconomic metrics, depending on the strategies employed and ecological settings. Thus, we synthesized different restoration strategies and discussed the environmental and socioeconomic factors influencing restoration success. In the present systematic review, [...] Read more.
The effectiveness of restoration programs may differ in terms of environmental and socioeconomic metrics, depending on the strategies employed and ecological settings. Thus, we synthesized different restoration strategies and discussed the environmental and socioeconomic factors influencing restoration success. In the present systematic review, we examined peer-reviewed articles published between 1990 and 2024 that discussed strategies and factors impacting land-based restoration. Only 227 of 55,229 articles from ScienceDirect, PubMed, and Google Scholar databases met the inclusion criteria. China, Brazil, and India are more active in conducting studies about land restoration compared to other regions, particularly in megadiverse countries in Asia with high degradation rates. There is a strong emphasis on practical and adaptive restoration strategies, including soil and water management (e.g., agroforestry), the use of native plant species, and weed and invasive species management. The prevalence of Acacia, Leucaena, and Eucalyptus species in restoration programs can inform decisions about effective species selection. Here, a holistic understanding of the complex ecological processes is crucial for the development of effective restoration strategies. Although policy frameworks have received less attention in restoration research, their incorporation into restoration projects can help address policy implications for land-based restoration. Overall, successful restoration necessitates a thorough understanding of the optimal strategies and environmental and socioeconomic factors impacting restoration success. Future restoration initiatives can leverage such an understanding to ensure successful implementation. Full article
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26 pages, 6580 KiB  
Article
Aspergillus brasiliensis E_15.1: A Novel Thermophilic Endophyte from a Volcanic Crater Unveiled through Comprehensive Genome-Wide, Phenotypic Analysis, and Plant Growth-Promoting Trails
by Amanda Martirena-Ramírez, José Germán Serrano-Gamboa, Yordanis Pérez-Llano, Claribel Orquídea Zenteno-Alegría, Mario León Iza-Arteaga, María del Rayo Sánchez-Carbente, Ana María Fernández-Ocaña, Ramón Alberto Batista-García and Jorge Luis Folch-Mallol
J. Fungi 2024, 10(8), 517; https://doi.org/10.3390/jof10080517 - 25 Jul 2024
Viewed by 382
Abstract
Thermophilic fungi have been seldom studied despite the fact that they can contribute to understanding ecological mechanisms of adaptation in diverse environments and have attractive toolboxes with a wide range of biotechnological applications. This work describes for the first time an endophytic and [...] Read more.
Thermophilic fungi have been seldom studied despite the fact that they can contribute to understanding ecological mechanisms of adaptation in diverse environments and have attractive toolboxes with a wide range of biotechnological applications. This work describes for the first time an endophytic and thermophilic strain of Aspergillus brasiliensis that was isolated in the crater of the active volcano “El Chichonal” in Mexico. This strain was capable of surviving in soil with a temperature of 60 °C and a pH of neutral acidity, which preluded a high thermostability and a potential in industrial application. The complete genome of A. brasiliensis E_15.1 was sequenced and assembled in 37 Mb of genomic DNA. We performed a comprehensive phylogenomic analysis for the precise taxonomic identification of this species as a novel strain of Aspergillus brasiliensis. Likewise, the predicted coding sequences were classified according to various functions including Carbohydrate-Active Enzymes (CAZymes), biosynthetic gene clusters of secondary metabolites (BGCs), and metabolic pathways associated with plant growth promotion. A. brasiliensis E_15.1 was found to degrade chitin, chitooligosaccharides, xylan, and cellulose. The genes to biosynthesize clavaric acid (a triterpene with antitumor activity) were found, thus probably having antitumor activity. In addition to the genomic analysis, a set of enzymatic assays confirmed the thermostability of extracellular xylanases and cellulases of A. brasiliensis E_15.1. The enzymatic repertoire of A. brasiliensis E_15.1 suggests that A. brasiliensis E_15.1 has a high potential for industrial application due to its thermostability and can promote plant growth at high temperatures. Finally, this strain constitutes an interesting source of terpenoids with pharmacological activity. Full article
(This article belongs to the Special Issue Extremophile Fungi: An Arising Novel Field in Perspective)
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22 pages, 745 KiB  
Review
Homoeologs in Allopolyploids: Navigating Redundancy as Both an Evolutionary Opportunity and a Technical Challenge—A Transcriptomics Perspective
by Gaetano Aufiero, Carmine Fruggiero, Davide D’Angelo and Nunzio D’Agostino
Genes 2024, 15(8), 977; https://doi.org/10.3390/genes15080977 - 24 Jul 2024
Viewed by 353
Abstract
Allopolyploidy in plants involves the merging of two or more distinct parental genomes into a single nucleus, a significant evolutionary process in the plant kingdom. Transcriptomic analysis provides invaluable insights into allopolyploid plants by elucidating the fate of duplicated genes, revealing evolutionary novelties [...] Read more.
Allopolyploidy in plants involves the merging of two or more distinct parental genomes into a single nucleus, a significant evolutionary process in the plant kingdom. Transcriptomic analysis provides invaluable insights into allopolyploid plants by elucidating the fate of duplicated genes, revealing evolutionary novelties and uncovering their environmental adaptations. By examining gene expression profiles, scientists can discern how duplicated genes have evolved to acquire new functions or regulatory roles. This process often leads to the development of novel traits and adaptive strategies that allopolyploid plants leverage to thrive in diverse ecological niches. Understanding these molecular mechanisms not only enhances our appreciation of the genetic complexity underlying allopolyploidy but also underscores their importance in agriculture and ecosystem resilience. However, transcriptome profiling is challenging due to genomic redundancy, which is further complicated by the presence of multiple chromosomes sets and the variations among homoeologs and allelic genes. Prior to transcriptome analysis, sub-genome phasing and homoeology inference are essential for obtaining a comprehensive view of gene expression. This review aims to clarify the terminology in this field, identify the most challenging aspects of transcriptome analysis, explain their inherent difficulties, and suggest reliable analytic strategies. Furthermore, bulk RNA-seq is highlighted as a primary method for studying allopolyploid gene expression, focusing on critical steps like read mapping and normalization in differential gene expression analysis. This approach effectively captures gene expression from both parental genomes, facilitating a comprehensive analysis of their combined profiles. Its sensitivity in detecting low-abundance transcripts allows for subtle differences between parental genomes to be identified, crucial for understanding regulatory dynamics and gene expression balance in allopolyploids. Full article
(This article belongs to the Special Issue Genetics and Genomics of Polyploid Plants)
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Article
Effects of Fuel Removal on the Flammability of Surface Fuels in Betula platyphylla in the Wildland–Urban Interface
by Xintong Chen, Mingyu Wang, Baozhong Li, Lixuan Wang, Jibin Ning, Guang Yang and Hongzhou Yu
Fire 2024, 7(7), 261; https://doi.org/10.3390/fire7070261 - 22 Jul 2024
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Abstract
This paper aimed to provide technical support for fuel management by exploring different strengths of fuel removal on the physical and chemical properties and flammability of Betula platyphylla forests in the wildland–urban interface. After investigating the northeastern region during the forest fire prevention [...] Read more.
This paper aimed to provide technical support for fuel management by exploring different strengths of fuel removal on the physical and chemical properties and flammability of Betula platyphylla forests in the wildland–urban interface. After investigating the northeastern region during the forest fire prevention period in May 2023, a typical WUI area was selected, and three different treatment strengths, combined with a control, were set up to carry out indoor and outdoor experiments for 27 weeks. Compared with previous studies, this study mainly investigated and analyzed the dynamic changes in the physical and chemical properties and fuel flammability after different intensities of treatments on a time scale. By processing and analyzing the data, the following results were obtained. Significant differences existed in the fuel loading of different time-lag fuels over time (p < 0.05). The ash and ignition point of 1 h time-lag fuel after different treatment intensities generally increased first and then decreased, and the higher heat value and ash-free calorific value generally decreased first and then increased. The physical and chemical properties of 10 h and 100 h time-lag fuel fluctuated with time, but the overall change was insignificant. The indicator that had the greatest impact on the combustion comprehensive score for different time-lag fuels was fuel loading. The change in the flammability of dead surface fuel with time varied significantly, and different treatment intensities effectively reduced the fuel’s flammability. The reduction effects, presented in descending order, were as follows: medium-strength treatment > low-strength treatment > high-strength treatment > control check. In conclusion, different treatment intensities have significant effects on the flammability of the fuel, and the medium-strength treatment has the best effect. Considering the ecological and economic benefits, adopting the medium-strength treatment for the WUI to regulate the fuel is recommended. Full article
(This article belongs to the Special Issue Forest Fuel Treatment and Fire Risk Assessment)
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