Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model
Abstract
:1. Introduction
2. Materials and Methods
3. Results and Discussion
4. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
Abbreviations
AFM | atomic force microscope |
BET | Brunauer–Emmett–Teller |
CD | circular dichroism |
CRISPR | clustered regularly interspaced short palindromic repeat |
DAPI | 4′,6-diamidino-2-phenylindole |
dCas9a | denatured CRISPR-associated protein 9 with activator |
DLS | dynamic light scattering |
EVAL | poly(ethylene-co-vinyl alcohol), |
HEK293T | human embryonic kidney 293 T cell line |
HPLC | high-performance liquid chromatography |
ITC | isothermal titration calorimetry |
MMIPs | magnetic molecularly imprinted composite nanoparticles |
MNIPs | magnetic non-imprinted polymeric particles |
MNPs | magnetic nanoparticles |
P5 | YVGSKTKEGVVHGVA |
P6 | YVGSKTKEGVVHGVT |
P7 | YVGSKTKKGVVHGVT |
PD | Parkinson’s disease |
QDs | quantum dots |
SNCA | α-synuclein |
SQUID | superconducting quantum interference device |
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Lee, M.-H.; Jan, J.-S.; Thomas, J.L.; Shih, Y.-P.; Li, J.-A.; Lin, C.-Y.; Ooya, T.; Barna, L.; Mészáros, M.; Harazin, A.; et al. Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model. Cells 2022, 11, 2584. https://doi.org/10.3390/cells11162584
Lee M-H, Jan J-S, Thomas JL, Shih Y-P, Li J-A, Lin C-Y, Ooya T, Barna L, Mészáros M, Harazin A, et al. Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model. Cells. 2022; 11(16):2584. https://doi.org/10.3390/cells11162584
Chicago/Turabian StyleLee, Mei-Hwa, Jeng-Shiung Jan, James L. Thomas, Yuan-Pin Shih, Jin-An Li, Chien-Yu Lin, Tooru Ooya, Lilla Barna, Mária Mészáros, András Harazin, and et al. 2022. "Cellular Therapy Using Epitope-Imprinted Composite Nanoparticles to Remove α-Synuclein from an In Vitro Model" Cells 11, no. 16: 2584. https://doi.org/10.3390/cells11162584