Expression of Functional Cannabinoid Type-1 (CB1) Receptor in Mitochondria of White Adipocytes
Abstract
:1. Introduction
2. Materials and Methods
2.1. Animal and Drugs
2.2. Sample Preparation for Morphological Studies
2.3. Peroxidase Immunohistochemistry
2.4. Immunogold Post-Embedding Technique
2.5. Morphometric Analysis
2.6. Homogenate Preparation of eWAT and iBAT Mouse Tissue
2.7. Complex I Assay Activity
2.8. Mitochondrial Isolation from eWAT Rat Tissue
2.9. Mitochondrial Respiration
2.10. Western Blotting
2.11. Statistical Analysis
3. Results
3.1. Immunohistochemical Detection of CB1 in Adipose Tissue
3.2. CB1 Receptor Is Present on Plasma Membrane and Associated to Nucleus and Mitochondria in eWAT Adipocytes
3.3. CB1 Receptor Activation Impacts Mitochondrial Respiration in eWAT
3.4. mtCB1 Reduces Mitochondrial Respiration in eWAT via sAC and PKA Activity
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pagano Zottola, A.C.; Severi, I.; Cannich, A.; Ciofi, P.; Cota, D.; Marsicano, G.; Giordano, A.; Bellocchio, L. Expression of Functional Cannabinoid Type-1 (CB1) Receptor in Mitochondria of White Adipocytes. Cells 2022, 11, 2582. https://doi.org/10.3390/cells11162582
Pagano Zottola AC, Severi I, Cannich A, Ciofi P, Cota D, Marsicano G, Giordano A, Bellocchio L. Expression of Functional Cannabinoid Type-1 (CB1) Receptor in Mitochondria of White Adipocytes. Cells. 2022; 11(16):2582. https://doi.org/10.3390/cells11162582
Chicago/Turabian StylePagano Zottola, Antonio C., Ilenia Severi, Astrid Cannich, Philippe Ciofi, Daniela Cota, Giovanni Marsicano, Antonio Giordano, and Luigi Bellocchio. 2022. "Expression of Functional Cannabinoid Type-1 (CB1) Receptor in Mitochondria of White Adipocytes" Cells 11, no. 16: 2582. https://doi.org/10.3390/cells11162582