RNA-Seq Analysis of Protection against Chronic Alcohol Liver Injury by Rosa roxburghii Fruit Juice (Cili) in Mice
Abstract
:1. Introduction
2. Materials and Methods
2.1. Cili, Liquid Diets and Chemicals
2.2. Experimental Animals
2.3. Serum Enzyme Activities
2.4. Triglyceride Determination
2.5. Haematoxylin and Eosin Staining and Oil-Red O Staining
2.6. RNA Extraction and Easy RNA-Seq
2.7. Principal Component Analysis
2.8. Heatmap Visualization of Differentially Expressed Genes
2.9. Real-Time qPCR
2.10. Statistics
3. Results
3.1. Cili Protected against Alcohol-Induced Elevation of Triglyceride
3.2. Cili Improved Alcohol-Induced Pathology and Lipid Accumulation
3.3. Cili Reversed Alcohol-Induced Aberrant Gene Expression
3.4. The qPCR Analysis of Selected Genes
4. Discussion
4.1. Cili Protected against Chronic Alcohol Liver Injury
4.2. Cili Alleviated Chronic Alcohol-Induced Aberrant Gene Expression
4.3. Multiple Components in Cili Could Function in an Intergraded Manner to Achieve Beneficial Effects
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Yang, S.; Huang, X.-Y.; Zhou, N.; Wu, Q.; Liu, J.; Shi, J.-S. RNA-Seq Analysis of Protection against Chronic Alcohol Liver Injury by Rosa roxburghii Fruit Juice (Cili) in Mice. Nutrients 2022, 14, 1974. https://doi.org/10.3390/nu14091974
Yang S, Huang X-Y, Zhou N, Wu Q, Liu J, Shi J-S. RNA-Seq Analysis of Protection against Chronic Alcohol Liver Injury by Rosa roxburghii Fruit Juice (Cili) in Mice. Nutrients. 2022; 14(9):1974. https://doi.org/10.3390/nu14091974
Chicago/Turabian StyleYang, Shan, Xian-Yu Huang, Nian Zhou, Qin Wu, Jie Liu, and Jing-Shan Shi. 2022. "RNA-Seq Analysis of Protection against Chronic Alcohol Liver Injury by Rosa roxburghii Fruit Juice (Cili) in Mice" Nutrients 14, no. 9: 1974. https://doi.org/10.3390/nu14091974