Comparative Analysis of the Fatty Acid Profiles of Selected Representatives of Chlorella-Clade to Evaluate Their Biotechnological Potential
Abstract
:1. Introduction
2. Materials and Methods
2.1. Isolation and Cultivation of Algal Strains
2.2. Microscopy
2.3. DNA Isolation, Amplification, Purification, and Sequencing
2.4. Methods of Phylogenetic Analysis
2.5. Fatty Acid Extraction and Analysis
2.6. Statistical Analysis
3. Results
3.1. Morphological Observations by Light Microscopy
3.2. Phylogenetic Analysis
3.3. Fatty Acid Composition of the Studied Organisms
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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C. vulgaris VKM Al-335 | Mc. lacustre VKM Al-343 | Mc. Thermotolerans VKM Al-332 | Mr. similis VKM Al-346 | Mr. krienitzii VKM Al-428 | T. aestivum | |
---|---|---|---|---|---|---|
16-carbon FA, % | 59.15 | 62.27 ** | 54.44 | 43.76 | 35.50 | 24.59 |
18-carbon FA, % | 34.06 | 34.98 | 41.83 | 49.66 | 59.36 ** | 75.41 |
MUFA, % | 4.85 | 5.91 | 3.18 | 9.08 ** | 6.06 | 6.07 |
PUFA, % | 44.09 | 73.77 | 68.40 | 70.11 | 75.37 ** | 75.41 |
UFA, % | 48.94 | 79.68 | 71.58 | 79.19 | 81.43 ** | 81.48 |
UI | 1.31 | 2.39 ** | 1.95 | 1.84 | 1.85 | 2.25 |
ω-3 FA, % | 31.69 | 63.85 ** | 51.16 | 34.87 | 28.32 | 67.75 |
ω-6 FA, % | 12.40 | 9.91 | 17.24 | 35.24 | 47.04 ** | 7.66 |
ω-3/ω-6 | 2.56 | 6.44 ** | 2.97 | 0.99 | 0.60 | 8.84 |
C. vulgaris VKM Al-335 | Mc. lacustre VKM Al-343 | Mc. thermo-tolerans VKM Al-332 | Mr. similis VKM Al-346 | Mr. krienitzii VKM Al-428 | |
---|---|---|---|---|---|
Minor straight-chain fatty acids | |||||
Tetradecanoic acid | 1.18 ± 0.20 | 0.99 ± 0.07 | 0.97 ± 0.04 | 0.45 ± 0.24 | 0.28 ± 0.02 |
Pentadecanoic acid | 0.84 ± 0.02 | 1.69 ± 0.96 | |||
Branched-chain fatty acids | |||||
Tetradecanoic acid, 12-methyl- | 0.52 ± 0.04 | 1.07 ± 1.05 | 0.24 ± 0.21 | ||
Hexadecanoic acid, 14-methyl- | 0.68 ± 0.05 | 0.83 ± 0.55 | |||
Hexadecanoic acid, 15-methyl- | 1.32 ± 0.03 | 1.20 ± 0.09 | |||
Heptadecanoic acid, 16-methyl- | 1.45 ± 0.25 | 1.53 ± 0.31 | |||
Octadecanoic acid, 17-methyl- | 1.18 ± 0.46 | ||||
Cyclic fatty acids | |||||
[1,1′-Bicyclopropyl]-2-octanoic acid, 2′-hexyl- | 0.47 ± 0.19 | 2.04 ± 1.52 | 0.68 ± 0.47 | 0.84 ± 0.96 | |
Cyclopropaneoctanoic acid, 2-[[2-[(2-ethylcyclopropyl)methyl] cyclopropyl]methyl] | 3.00 ± 0.13 | ||||
Cyclopropanetetradecanoic acid, 2-octyl- | 0.40 ± 0.07 |
14:0 | 15:0 | 16:0 | 16:1Δ7 | 16:2Δ7,10 | 16:3Δ7,10,13 | 16:4Δ4,7,10,13 | 18:1Δ9 | 18:2Δ9,12 | 18:3Δ9,12,15 | 18:3Δ6,9,12 | |
---|---|---|---|---|---|---|---|---|---|---|---|
14:0 | 1 | −0.392 | 0.945 | −0.462 | −0.922 | −0.145 | 0.084 | −0.348 | −0.845 | 0.407 | 0.973 |
15:0 | −0.392 | 1 | −0.338 | −0.129 | 0.604 | 0.544 | −0.374 | 0.708 | 0.223 | −0.220 | −0.387 |
16:0 | 0.945 *** | −0.338 | 1 | −0.659 | −0.784 | −0.285 | −0.230 | −0.212 | −0.647 | 0.181 | 0.953 |
16:1Δ7 | −0.462 | −0.129 | −0.659 * | 1 | 0.252 | −0.107 | 0.747 | 0.081 | 0.221 | −0.094 | −0.617 |
16:2Δ7,10 | −0.922 *** | 0.604 | −0.784 ** | 0.252 | 1 | 0.053 | −0.378 | 0.648 | 0.894 | −0.640 | −0.904 |
16:3Δ7,10,13 | −0.145 | 0.544 | −0.285 | −0.107 | 0.053 | 1 | 0.176 | −0.191 | −0.323 | 0.665 | −0.061 |
16:4Δ4,7,10,13 | 0.084 | −0.374 | −0.230 | 0.747 | −0.378 | 0.176 | 1 | −0.443 | −0.440 | 0.542 | −0.036 |
18:1Δ9 | −0.348 | 0.708 | −0.212 | 0.081 | 0.648 | −0.191 | −0.443 | 1 | 0.493 | −0.789 | −0.444 |
18:2Δ9,12 | −0.845 ** | 0.223 | −0.647 | 0.221 | 0.894 ** | −0.323 | −0.440 | 0.493 | 1 | −0.773 | −0.815 |
18:3Δ9,12,15 | 0.407 | −0.220 | 0.181 | −0.094 | −0.640 | 0.665 * | 0.542 | −0.789 ** | −0.773 ** | 1 | 0.470 |
18:3Δ6,9,12 | 0.973*** | −0.387 | 0.953 *** | −0.617 | −0.904 ** | −0.061 | −0.036 | −0.444 | −0.815 * | 0.470 | 1 |
C. vulgaris VKM Al-335 | Mc. lacustre VKM Al-343 | Mc.thermo-tolerans VKM Al-332 | Mr. similis VKM Al-346 | Mr. krienitzii VKM Al-428 | |
---|---|---|---|---|---|
cis-9,10-epoxyoctadecanoic acid | + | − | − | − | − |
dodecanoic acid, 3-hydroxy- | − | − | − | + | + |
7-methyl-Z-tetradecen-1-ol acetate | + | − | − | − | − |
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Krivina, E.; Degtyaryov, E.; Tebina, E.; Temraleeva, A.; Savchenko, T. Comparative Analysis of the Fatty Acid Profiles of Selected Representatives of Chlorella-Clade to Evaluate Their Biotechnological Potential. Int. J. Plant Biol. 2024, 15, 837-854. https://doi.org/10.3390/ijpb15030060
Krivina E, Degtyaryov E, Tebina E, Temraleeva A, Savchenko T. Comparative Analysis of the Fatty Acid Profiles of Selected Representatives of Chlorella-Clade to Evaluate Their Biotechnological Potential. International Journal of Plant Biology. 2024; 15(3):837-854. https://doi.org/10.3390/ijpb15030060
Chicago/Turabian StyleKrivina, Elena, Evgeny Degtyaryov, Elizaveta Tebina, Anna Temraleeva, and Tatyana Savchenko. 2024. "Comparative Analysis of the Fatty Acid Profiles of Selected Representatives of Chlorella-Clade to Evaluate Their Biotechnological Potential" International Journal of Plant Biology 15, no. 3: 837-854. https://doi.org/10.3390/ijpb15030060