Search Results (46)

Tiger nut is the tuber of the perennial herbaceous plant Cyperus esculentus L., whose unique underground tubers are not only the main reproductive organ but also an important oil storage site. Oleosin is the most abundant structural protein in the oil body, which is an important membrane structural protein, playing a role in the formation and stability of lipid droplets in oilseed crops. Most studies have focused on the oleosin in oilseeds, but rarely on the oil containing tuber. In this study, nine oleosin genes from the Cyperus esculentus transcriptome were identified and divided into two groups via phylogenetic analysis. The expression patterns of the nine oleosins were examined through quantitative real-time PCR (qRT-PCR) in various development stages of stem tissue (35 d, 50 d, 75 d, 90 d, and 120 d after sowing). The subcellular localization of CeOle4 indicated that this protein was localized exclusively to membrane, indicating that it functioned in the plasma membrane. The highly expressed gene CeOle4 within the CeOleosin gene family was further transformed into yeast cells and plant materials. The results demonstrate that CeOle4 can promote lipid synthesis, enhancing the stability of oil lipids at low temperature and changing seed phenotypic traits. This discovery addresses and enriches the research on the function of CeOleosin genes and lays the groundwork for future studies on novel and superior transgenic crops related to tiger nut. Full article

This study presents for the first time functional cookies for diabetics made with 100% organic Cyperus esculentus L. flour, either plain or enhanced with 5% polyfloral honey syrup containing the probiotic strain Lacticaseibacillus rhamnosus SL42. The flour’s chemical composition and rheological and functional properties were analyzed, and 33 diabetic and semi-naive panelists assessed the cookies’ sensory properties. MRS-cys agar cultures and SEM analysis evaluated SL42 survival and adhesion capacity over 21 days at 25 °C. Results showed that the flour and its extracts are rich in polyphenols and flavonoids, indicating strong antioxidant and antibacterial properties. Both cookie types met international standards when compared to commercial cookies and had similar physical properties. Sensory evaluation on day 1 revealed higher quality indicators for honey syrup-enriched cookies, but after 15 days, control cookies were preferred. The CIE LAB analysis confirmed the dietetic flour’s typical dark color, with honey syrup-enriched cookies being darker. Despite textural differences, both cookies maintained detectable crispness over storage. Honey syrup-enriched cookies effectively carried L. rhamnosus SL42, remaining viable at 6.43 Log CFU per cookie after 21 days and adhering to the cookie’s surface, as confirmed by SEM analysis. Further research is recommended to better understand the therapeutic value of these cookies. Full article

This study aimed to enhance the efficiency of tiger nut combine harvesters by reducing impurity and loss rates during processing. Scholars focused on analyzing the composition and suspension speed of the bean mixture, leading to the development of a wind-screen impurity-removal method. The wind-screen-type bean-separation device was designed with a cross-flow fan, louver screen, frame, and driving mechanism. Theoretical analysis was employed to discuss the motion characteristics and behavior of the sieve body and material, thereby revealing the screening dynamics of tiger nuts and impurities. Factors such as crank radius, crank speed, and fan speed were identified as crucial for optimizing separation performance. Initial single-factor tests helped narrow down the range of influencing factors. Subsequently, a three-factor, three-level Box–Behnken test was conducted with crank radius, crank speed, and fan speed as variables and impurity rate and loss rate as evaluation indexes. This led to the establishment of a multiple regression equation linking these factors to the evaluation indexes. Through response surface analysis and multi-objective optimization using the regression model, the optimal operational parameters for the device were determined: crank radius of 45 mm, crank speed of 497 r/min, and the fan speed of 1100 r/min. Theoretical calculations predicted an impurity rate of 2.42% and a loss rate of 0.51%. Verification tests confirmed these findings, showing an average impurity rate of 2.53% and a loss rate of 0.56%, which met the mechanized harvesting standards for tiger nuts. Overall, this study introduces a novel method and technical framework for effectively separating tiger nuts from impurities, thereby advancing the mechanization of tiger nut harvesting processes. Full article

Intercropping is a vital cropping system that can create a conducive growth environment for crops and enhance land productivity. Tiger nuts (Cyperus esculentus L.) have high oil content and are adaptable to various soil types, making them a promising new oil crop with significant development potential. This study evaluated the plant height, leaf area, tiller numbers, biomass, land equivalent ratio (LER), and root morphological characteristics of tiger nuts. The agronomic traits and root distribution of tiger nuts and other crops were further investigated to achieve the goal of high yield for tiger nuts. Seven intercropping systems were implemented in the experiment: maize–tiger nut intercropping (MT), soybean–tiger nut intercropping (ST), cotton–tiger nut intercropping (CT), monoculture tiger nut (T), monoculture maize (M), monoculture soybean (S), and monoculture cotton (C). The results indicated that under different planting systems, the agronomic traits of tiger nuts in MT and ST modes were superior, with plant height and tiller numbers increasing by 7.6% to 11.6%. However, the plant height and Soil Plant Analysis Development (SPAD) values in CT mode were slightly lower than in T mode. Additionally, intercropping reduced the leaf area by 6.2% to 37.9%. Root development was more pronounced in intercropping modes, with the ST mode showing the most significant improvement, increasing the 0–20 cm root length density (RLD) by 12.2% to 45.7%. Therefore, each of the three intercropping modes demonstrated distinct advantages. The LER of the intercropping systems ranged from 1.10 to 1.24, enhancing land utilization, with tiger nuts being the dominant species. Compared to monoculture, the ST mode exhibited the best overall effect. Understanding the impact of different planting systems on tiger nuts provides valuable insights for developing tiger nut cultivation in Xinjiang. Full article

The tiger nut (Cyperus esculentus L.) is a usable tuber and edible oil plant. The size of the tubers is a key trait that determines the yield and the mechanical harvesting of tiger nut tubers. However, little is known about the anatomical and molecular mechanisms of tuber expansion in tiger nut plants. This study conducted anatomical and comprehensive transcriptomics analyses of tiger nut tubers at the following days after sowing: 40 d (S1); 50 d (S2); 60 d (S3); 70 d (S4); 90 d (S5); and 110 d (S6). The results showed that, at the initiation stage of a tiger nut tuber (S1), the primary thickening meristem (PTM) surrounded the periphery of the stele and was initially responsible for the proliferation of parenchyma cells of the cortex (before S1) and then the stele (S2–S3). The increase in cell size of the parenchyma cells occurred mainly from S1 to S3 in the cortex and from S3 to S4 in the stele. A total of 12,472 differentially expressed genes (DEGs) were expressed to a greater extent in the S1–S3 phase than in S4–S6 phase. DEGs related to tuber expansion were involved in cell wall modification, vesicle transport, cell membrane components, cell division, the regulation of plant hormone levels, signal transduction, and metabolism. DEGs involved in the biosynthesis and the signaling of indole-3-acetic acid (IAA) and jasmonic acid (JA) were expressed highly in S1–S3. The endogenous changes in IAA and JAs during tuber development showed that the highest concentrations were found at S1 and S1–S3, respectively. In addition, several DEGs were related to brassinosteroid (BR) signaling and the G-protein, MAPK, and ubiquitin–proteasome pathways, suggesting that these signaling pathways have roles in the tuber expansion of tiger nut. Finally, we come to the conclusion that the cortex development preceding stele development in tiger nut tubers. The auxin signaling pathway promotes the division of cortical cells, while the jasmonic acid pathway, brassinosteroid signaling, G-protein pathway, MAPK pathway, and ubiquitin protein pathway regulate cell division and the expansion of the tuber cortex and stele. This finding will facilitate searches for genes that influence tuber expansion and the regulatory networks in developing tubers. Full article

To tackle problems such as the difficult separation from sand and the high power consumption of tiger nut harvesting in the sandy areas of Xinjiang, a conveying and separating device for tiger nut harvesters was designed. The axial and radial migrations of materials under screw action and the separation process of materials under vibratory action were analyzed dynamically. A simulation analysis was carried out on the conveying and separating process based on EDEM software. The migration trajectories of tiger nuts and sand particles were extracted, the displacement variations of sand particles on the X-axis, Y-axis, and Z-axis were analyzed in the action area of the screen-cleaning spike teeth and the screw action area, respectively, and the conveying and separation law of the tiger nut harvest mixture was clarified. With key parameters such as the screw velocity ratio, amplitude, vibration frequency, and machine operation velocity as test factors, and with the sand removal rate, crushing rate, and power consumption as test evaluation indicators, a four-factor, five-level orthogonal central composite test design was implemented. The test results were analyzed via the regression variance analysis method, and relation models between variable factors and evaluation indicators were constructed. The test results show that under the combined conditions of a screw velocity ratio of 0.88, an amplitude of 4.7 mm, a vibration frequency of 7.5 Hz, and a machine operation velocity of 0.92 km/h, the sand removal rate is 90.40%, the crushing rate is 1.66%, and the power consumption is 2.24 kW in theory. The optimized results were verified by tests. The sand removal rate was 88.92%, the crushing rate was 1.71%, the total power consumption was 2.29 kW, and the errors from the predicted values were 1.6%, 3.0%, and 2.2%, respectively, meeting the requirements for tiger nut harvesting conveyance and separation. This research can provide support for the development of technology and equipment for mechanized harvesting of tiger nuts in the sandy areas of Xinjiang. Full article

The cultivation of tiger nut (Cyperus esculentus L.) on marginal lands is a feasible and effective way to increase food production in Northern China. However, the specific influence of nitrogen fertilizer application on the growth dynamics, tuber expansion, overall yield, and nitrogen use efficiency (NUE) of tiger nuts cultivated on these sandy lands is yet to be fully elucidated. From 2021 to 2022, we conducted a study to determine the effect of N fertilizers on the leaf function morphology, canopy apparent photosynthesis (CAP), tuber yield, and NUE of tiger nut. The results indicate that the tuber yield and NUE are closely related to the specific leaf area (SLA), leaf area index (LAI), leaf nitrogen concentration per area (N_A), CAP, and tuber expansion characteristics. Notably, significant enhancements in the SLA, LAI, N_A, and CAP during the tuber expansion phase ranging from the 15th to the 45th day under the 300 kg N ha⁻¹ treatment were observed, subsequently leading to increases in both the tuber yield and NUE. Moreover, a maximum average tuber filling rate was obtained under the N300 treatment. These improvements led to substantial increases in the tuber yield (32.1–35.5%), nitrogen agronomic efficiency (NAE, 2.1–5.3%), nitrogen partial factor productivity (NPP, 4.8–8.1%), and nitrogen recovery efficiency (NRE, 3.4–5.7%). Consequently, 300 kg N ha⁻¹ of N fertilizers is the most effective dose for optimizing both the yield of tiger nut tubers and the NUE of tiger nut plants in marginal soils. Structural equation modeling reveals that N application affects the yield and NUE through its effects on leaf functional traits, the CAP, and the tuber filling characteristics. Modeling indicates that tuber expansion characteristics primarily impact the yield, while CAP predominantly governs the NUE. Above all, this study highlights the crucial role of N fertilizer in maximizing the tiger nut tuber yield potential on marginal lands, providing valuable insights into sustainable farming in dry areas. Full article

In order to solve the problem of inconsistent moisture content in particles during the drying process of tiger nuts (Cyperus esculentus) due to uneven air flow and temperature distribution in the drying chamber, an open-hole corner box was designed based on the principle of negative pressure micro-perforated air supply. Using computational fluid dynamics (CFD) and discrete element method (DEM) simulation, coupled with the basic theory of interphase heat and mass transfer, a mathematical model for interphase heat and moisture coupling transfer was established. The effects of different aperture rates of corner boxes in the drying chamber, spatial location arrangement, and other related variables on the airfield distribution, temperature field distribution, tiger nut temperature, and moisture content changes were investigated. The results show that the average air velocity below the air inlet gradually increases as the opening ratio increases. When the opening rate is 0.33%, the wind field uniformity is better, and the inhomogeneity of the drying chamber wind field is improved. As the lateral distance increases, the consistency of the moisture content distribution increases and then decreases, and the flow rate of the tiger nuts gradually increases when the grain is discharged. The rate of decrease in water content decreases gradually with the increase in longitudinal distance. When the wind speed reaches 4 m/s, the drying chamber wind field is more uniform, and the water vapor diffusion efficiency at the outlet is basically the same. Therefore, the appropriate corner box has a horizontal distance of 320 mm and a longitudinal distance of 420 mm, providing a basis for the design of tiger nut drying equipment. Full article

Tiger nut (Cyperus esculentus) or chufa is little known plant of high nutritious content. Popularized by a plant-based drink called “horchata de chufa,” it still offers a lot to research, being abundant in fat, starch, fiber and minerals and vitamins. To properly adjust this raw material to new purposes, the knowledge of crucial properties of the most basic blends like with soft wheat flour is needed. This article evaluates the techno-functional, viscometrical and bioactive characteristics of soft wheat:tiger nut blends of 5%, 10%, 15%, 20% and 25%. Granulometry, water-holding capacity (WHC), water absorption capacity (WAC), water absorption index (WAI), water solubility index (WSI), oil absorption capacity (OAC), hydrophilic/lipophilic index (HLI), color, pasting properties, total polyphenol content (TPC), antioxidant activity (DPPH), reducing sugars content and dough-rising capacity were assessed. The addition of tiger nut improved total polyphenol content of blends, however, It was observed that the addition of tiger nuts raised the total polyphenol content of the mixtures, but this was not statistically significant despite as much as 25% of tiger nuts. Oppositely, antioxidant activity was gradually improved with increasing tiger nut content. Pasting properties were impacted by tiger nut addition, lowering both pasting viscosity and trough viscosity, however, final viscosity was not particularly affected, being lowered by less than 15%. The highest water absorption was noted for 100% tiger nut both for WHC and WAC, however, WAI was the lowest for this sample. All the blends with tiger nut revealed improved dough-rising profile. Full article

Nitrogen (N) is an essential element both affecting rhizosphere microorganisms within soil and supporting plant nutrition; however, little is known about how the rhizosphere microbial community composition of tiger nut in sandy soil responds to nitrogen addition. In this study, high-throughput sequencing technology is employed to analyze the shifts in composition and co-occurrence networks of rhizosphere microbial communities in tiger nut after nitrogen addition in sandy farmland. Results reveal that nitrogen addition significantly increases several soil parameters, including total organic matter (SOC, 32.2%), total nitrogen (TN, 46.2%), alkali-hydro nitrogen (AN, 92.7%), β-1,4-glucosidase (BG, 12.6%), L-leucine aminopeptidase (LAP, 8.62%), β-1,4-xylosidase(XYL, 25.6%), and β-1,4-N-acetylglucosaminidase (NAG, 32.3%). Meanwhile, bacterial α-diversity decreases with nitrogen addition, while fungi remain unaffected. Network analysis indicates a reduction in connections between microorganisms; however, increasing stability is observed in the interaction network after nitrogen addition. Importantly, nitrogen addition leads to the enhancement of rhizosphere soil multifunctionality, with fungal diversity identified as the primary driver of soil multifunctionality. The positive impact of microbial diversity on soil multifunctionality outweighs the relative negative effects. This study sheds light on the nuanced effects of nitrogen addition on rhizosphere microbial diversity and its consequent impact on soil multifunctionality, with Acidobacteria, Proteobacteria and Ascomycota having positive effects, providing a comprehensive understanding of the complex environmental–plant–soil–microbe interactions in sandy farmland ecosystems. Full article

In this study, Tiger nut (Cyperus esculentus L.) oil-based oleogels were prepared using the emulsion template method with whey protein (WPI; 0.5–2.5% (w/v) and Xanthan gum (XG; 0.1–0.5% (w/v). The microstructure of the oleogels obtained from the high internal phase emulsion (HIPE) and an emulsion after further shearing were observed using an optical microscope and laser confocal microscopy. A series of rheological tests were conducted to evaluate the effect of WPI and XG concentrations on the strength of the emulsion and oleogel. The texture, oil holding capacity, and oxidative stability of oleogels were characterized. The results showed that XG alone could not form oleogel, while the concentration of WPI had more effect than XG. When WPI was at a fixed concentration, the viscoelasticity of HIPE increased with the addition of XG. This was due to the complexation of WPI and XG, forming a stable gel network between the tight emulsion droplets and thus giving it a higher viscoelasticity. With an increase in WPI concentration, the stability and viscoelasticity of the emulsion were increased, and the oil-holding capacity and gel strength of the oleogels were enhanced. Moreover, the addition of XG could significantly enhance the stability and viscoelasticity of the emulsion (p < 0.05), and an increase in the concentration had a positive effect on it. The oleogels showed high gel strength (G′ > 15,000 Pa) and good thixotropic recovery when the XG concentration was higher than 0.3% (w/v). WPI (2.0%) and XG (>0.3%) could be used to obtain HIPE with good physicochemical and viscoelastic properties, which in turn lead to oleogels with minimal oil loss, viscoelastic and thixotropic recovery, and temperature stability. Compared with tiger nut oil-based oleogel, tiger nut oil contained more polyunsaturated fatty acids, which were more easily decomposed through oxidation during storage and had lower oxidation stability. This study provides a reference for the preparation of oleogels from food-approved polymers and provides additional theoretical support for their potential application as solid fat substitutes. Full article

The global COVID-19 pandemic has warned scientists of the requirement to look for new antimicrobial compounds to prevent infection by this type of viral pathogen. Natural compounds are becoming a promising avenue of research thanks to their renewable, biodegradable, and non-toxic properties. In this work, tiger nut milk’s (TNM) antiviral properties, with and without sugar, were studied against enveloped and non-enveloped viruses. The antiviral properties of TNM were evaluated at different concentrations. The antiviral tests showed that TNM is antiviral against the enveloped bacteriophage phi 6, which is commonly used as a surrogate for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), although it did not have any antiviral effect against the non-enveloped bacteriophage MS2. We also found that adding sugar to this natural drink can improve its antiviral properties against enveloped viruses and render it antiviral against non-enveloped viruses like bacteriophage MS2. The antiviral activity of TNM depends on the TNM concentration. TNM is a natural bioproduct that could help to fight against viral infections and protect against a wide range of viral illnesses. These results confirm that the typical sweetened drink made from tiger nut extract and sugar (known as horchata in Spain) possesses broad-spectrum antiviral properties. Full article

Tiger nuts are a non-genetically modified organism crop with high adaptability and economic value, and they are being widely promoted for cultivation in China. This study proposed a new yield-estimation method based on a lightweight convolutional neural network (CNN) named Squeeze Net to provide accurate production forecasts for tiger nut tubers. The multispectral unmanned aerial vehicle (UAV) images were used to establish phenotypic datasets of tiger nuts, comprising vegetation indices (VIs) and plant phenotypic indices. The Squeeze Net model with a lightweight CNN structure was constructed to fully explore the explanatory power of the spectral UAV-derived information and compare the differences between the parametric and nonparametric models applied in tiger nut yield predictions. Compared with stepwise multiple linear regression (SMLR), both algorithms achieved good yield prediction performances. The highest obtained accuracies reflected an R² value of 0.775 and a root-mean-square error (RMSE) value of 688.356 kg/ha with SMLR, and R² = 0.780 and RMSE = 716.625 kg/ha with Squeeze Net. This study demonstrated that Squeeze Net can efficiently process UAV multispectral images and improve the resolution and accuracy of the yield prediction results. Our study demonstrated the enormous potential of artificial intelligence (AI) algorithms in the precise crop management of tiger nuts in the arid sandy lands of northwest China by exploring the interactions between various intensive phenotypic traits and productivity. Full article

Rice, tiger nut and carob are Mediterranean products suitable for developing new foods, such as fermented beverages, due to their nutritional properties. These crops have a high carbohydrate content, are gluten and lactose-free and have a low allergenicity index. The development of fermented beverages from these crops can contribute to the Sustainable Development Goals by promoting human health and sustainable production and consumption. A narrative review of the nutritional value and potential functional activity of fermented beverages made from these crops was carried out. This literature review of existing studies on fermented and non-fermented beverages highlights their composition, production methodology, and health benefits. Fermented beverages made from these crops are high in fiber, essential fatty acids, vitamins (group B), and minerals. Fermentation increases the bioaccessibility of these nutrients while decreasing possible anti-nutritional factors. These fermented beverages offer several health benefits due to their antioxidant effects, modulating the intestinal microbiota and reducing the incidence of chronic degenerative diseases such as metabolic syndrome. Therefore, fermented rice, tiger nut and carob beverages can improve the Spanish diet by offering improved nutritional value and beneficial health effects. Additionally, these local crops promote sustainability, making them an appropriate choice for developing new fermented beverages. Full article

Tiger nut beverages are non-alcoholic products that are characterized by their pale color and soft flavor. Conventional heat treatments are widely used in the food industry, although heated products are often damaging to their overall quality. Ultra-high pressure homogenization UHPH) is an emerging technology that extends the shelf-life of foods while maintaining most of their fresh characteristics. The present work deals with the comparison of the effect of conventional thermal homogenization-pasteurization (H-P, 18 + 4 MPa at 65 °C, 80 °C for 15 s.) and UHPH (at 200 and 300 MPa, and inlet temperature of 40 °C), on the volatile composition of tiger nut beverage. Headspace-solid phase microextraction (HS-SPME) was used for detecting volatile compounds of beverages, which were then identified by gas chromatography-mass spectrometry (GC-MS). A total of 37 different volatile substances were identified in tiger nut beverages, which were primarily grouped into the aromatic hydrocarbons, alcohols, aldehydes and terpenes chemical families. Stabilizing treatments increased the total amount of volatile compounds (H-P > UHPH > R-P). H-P was the treatment that produced the most changes in the volatile composition of RP, while treatment at 200 MPa had a minor impact. At the end of their storage, these products were also characterized by the same chemical families. This study evidenced the UHPH technology as an alternative processing of tiger nut beverages production that minimally modifies their volatile composition. Full article