Search Results (8,520)

Background and Objectives: This study uses finite element analysis to evaluate the impact of abutment angulation, types, and framework materials on the stress distribution and fatigue performance of dental implant systems. Materials and Methods: Three-dimensional models of maxillary three-unit fixed implant-supported prostheses were analyzed. Abutments with different angles and types were used. Two different framework materials were used. Conducted on implants, a force of 150 N was applied obliquely, directed from the palatal to the buccal aspect, at a specific angle of 30 degrees. The distribution of stress and fatigue performance were then assessed, considering the types of restoration frameworks used and the angles of the abutments in three distinct locations. The simulation aspect of the research was carried out utilizing Abaqus Software (ABAQUS 2020, Dassault Systems Simulation Corp., Johnston, RT, USA). Results: In all models, fatigue strengths in the premolar region were higher than in the molar region. Maximum stress levels were seen in models with angled implants. In almost all models with the zirconia framework, fatigue performance was slightly lower. Conclusions: According to the findings of this study, it was concluded that the use of metal-framework multi-unit restorations with minimum angulation has significant positive effects on the biomechanics and long-term success of implant treatments. Full article

Layered rocks are widely distributed in mining and underground engineering. The evolution processes, such as crack initiation, development and penetration, inevitably occur due to stress changes. This study carried out an experiment and numerical simulation to explore the correspondence between crack distribution and bedding dip, and to reveal the mechanical mechanism of layered rock fracturing. The results show that the layered rock specimens with different bedding dips obtained different stress combinations under the same uniaxial compression conditions. There are a total of five types of stress combinations, including pure compression type, compression shear type, pure shear type, tension shear type, and pure tension type. The Mohr circle is effective in characterizing the relationship between the stress combinations and failure modes. The failure mode of layered rocks in the range of 0° to 150° is presented the variation features of “tensile failure → compression-shear failure → shear failure → tensile shear failure → tensile failure”. Furthermore, the combined distributions of dominant and secondary cracks are summarized into the penetrating mode, the exfoliation mode, the feather crack mode, and the associated mode in high-dip of layered marbles. This paper provides research ideas for stability monitoring and crack tracking of layered rock mass engineering. Full article

The Water Engineering Laboratory (WEL) of the University of Perugia hosts a real-scale water distribution network (WDN) with a service line comprising high-density polyethylene (HPDE) pipes and supplied by two pumps in series. The carried out unsteady-state tests show the WDN behavior during transients due to pump switching-on and -off. In particular, they underline the most pressure-stressed sections of the system. The obtained results can help water utility managers in protecting these sections with the aim to preserve the integrity of the WDN. Full article

Alfin-like proteins (ALs) form a plant-specific transcription factor (TF) gene family involved in the regulation of plant growth and development, and abiotic stress response. In this study, 30 ALs were identified in Brassica napus ecotype ‘Zhongshuang 11’ genome (BnaALs), and unevenly distributed on 15 chromosomes. Structural characteristic analysis showed that all of the BnaALs contained two highly conserved domains: the N terminal DUF3594 domain and the C-terminal PHD-finger domain. The BnaALs were classified into four groups (Group I-IV), supported by conserved intron–exon and protein motif structures in each group. The allopolyploid event between B. oleracea and B. rapa ancestors and the small-scale duplication events in B. napus both contributed to the large BnaALs expansion. The promoter regions of BnaALs contained multiple abiotic stress cis-elements. The BnaALs in I-IV groups were mainly expressed in cotyledon, petal, root, silique, and seed tissues, and the duplicated gene pairs shared highly similar expression patterns. RNA-seq and RT-qPCR analysis showed that BnaALs were obviously induced by low nitrogen (LN) and low phosphorus (LP) treatments in roots. Overexpressing BnaAL02 and BnaAL28 in Arabidopsis demonstrated their functions in response to LN and LP stresses. BnaAL28 enhanced primary roots’ (PRs) length and lateral roots’ (LRs) number under LP and LN conditions, where BnaAL02 can inhibit LR numbers under the two conditions. They can promote root hair (RH) elongation under LP conditions; however, they suppressed RH elongation under LN conditions. Our result provides new insight into the functional dissection of this family in response to nutrient stresses in plants. Full article

This study investigates bidirectionally sinusoidal corrugated steel shells using finite element analysis (FEA) software, primarily ABAQUS 2017, supplemented by RFEM 6.05 for an initial result comparison. The research aims to establish a robust numerical solution to understand the structural behaviour of these shells under static and dynamic loading. Starting with meticulous calculations for a selected structural element, the paper emphasises a comparative analysis between ABAQUS and RFEM, offering valuable insights into simulating the responses of corrugated steel shells. A distinctive contribution of this research lies in its novel and comprehensive parametric analysis of these shells under static and dynamic loading, an area that has not been explored in previous studies. The study systematically explores various model parameters, including geometrical and mechanical properties, with detailed analyses revealing their influences on deformation, stress distribution, dynamic properties, and behaviour. Another key feature is the development of a coded script that systematically generates diverse numerical models, allowing for a thorough exploration of the structural system’s response. This study advances our understanding of the structural behaviour of bidirectionally sinusoidal corrugated steel shells. These findings are set to enhance structural analysis and design practices by optimising parameters for improved performances in various engineering applications. Full article

This study aimed to investigate the mechanical behavior of resin composites and hybrid glass ionomer cement in class I adhesive dental restorations under loading and shrinkage conditions. Three CAD models of a mandibular first molar with class I cavities were created and restored with different techniques: a bi-layer of Equia Forte HT with Filtek One Bulk Fill Restorative composite (model A), a single layer of adhesive and Filtek One Bulk Fill Restorative (model B), and a single layer of Equia forte HT (model C). Each model was exported to computer-aided engineering software, and 3D finite element models were created. Models A and B exhibited a similar pattern of stress distribution along the enamel–restoration interface, with stress peaks of 12.5 MPa and 14 MPa observed in the enamel tissue. The sound tooth, B, and C models showed a similar trend along the interface between dentine and restoration. A stress peak of about 0.5 MPa was detected in the enamel of both the sound tooth and B models. Model C showed a reduced stress peak of about 1.2 MPa. A significant stress reduction in 4 mm deep class I cavities in lower molars was observed in models where non-shrinking dental filling materials, like the hybrid glass ionomer cement used in model C, were applied. Stress reduction was also achieved in model A, which employed a bi-layer technique with a shrinking polymeric filling material (bulk resin composite). Model C’s performance closely resembled that of a sound tooth. Full article

DnaJ proteins, also known as HSP40s, play a key role in plant growth and development, and response to environmental stress. However, little comprehensive research has been conducted on the DnaJ gene family in maize. Here, we identify 91 ZmDnaJ genes from maize, which are likely distributed in the chloroplast, nucleus, and cytoplasm. Our analysis revealed that ZmDnaJs were classified into three types, with conserved protein motifs and gene structures within the same type, particularly among members of the same subfamily. Gene duplication events have likely contributed to the expansion of the ZmDnaJ family in maize. Analysis of cis-regulatory elements in ZmDnaJ promoters suggested involvement in stress responses, growth and development, and phytohormone sensitivity in maize. Specifically, four cis-acting regulatory elements associated with stress responses and phytohormone regulation indicated a role in adaptation. RNA-seq analysis showed constitutive expression of most ZmDnaJ genes, some specifically in pollen and endosperm. More importantly, certain genes also responded to salt, heat, and cold stresses, indicating potential interaction between stress regulatory networks. Furthermore, early responses to heat stress varied among five inbred lines, with upregulation of almost tested ZmDnaJ genes in B73 and B104 after 6 h, and fewer genes upregulated in QB1314, MD108, and Zheng58. After 72 h, most ZmDnaJ genes in the heat-sensitive inbred lines (B73 and B104) returned to normal levels, while many genes, including ZmDnaJ55, 79, 88, 90, and 91, remained upregulated in the heat-tolerant inbred lines (QB1314, MD108, and Zheng58) suggesting a synergistic function for prolonged protection against heat stress. In conclusion, our study provides a comprehensive analysis of the ZmDnaJ family in maize and demonstrates a correlation between heat stress tolerance and the regulation of gene expression within this family. These offer a theoretical basis for future functional validation of these genes. Full article

We report on the use of open-air blade-coating as a scalable method for producing metal halide perovskite films with >10× fracture energy for durability and mechanical stability through the addition of corn starch polymer additives. This results in a manufacturable and robust perovskite that has tunable thicknesses exceeding 10 µm, among the highest reported values for solution-processed polycrystalline films. We find that an increasing amount of starch causes more uniform carbon distribution within the perovskite thickness as quantified by cross-sectional elemental composition measurements. Further, the incorporation of starch introduces beneficial compressive film stresses. Importantly, the optoelectronic behavior is not compromised, as the photoluminescence spectrum becomes more homogenous with the addition of corn starch up to 20% by weight. Full article

Marine soft clay is widely distributed in coastal areas. Aiming at the characteristics of low strength and stress level of marine soft clay, the effects of normal stress, water content, and resting time on the pile–soil interface shear characteristics of marine soft clay–jacked piles were investigated using improved direct shear test equipment. On this basis, a practical interface shear strength prediction model considering the above factors is proposed. The test results show that the relationship between shear stress and shear displacement at the pile–soil interface can be divided into three stages—initial, transitional, and stable—and the relationship is in accordance with the hyperbolic model. Under the same water content and resting time, the interface peak shear stress increases linearly with the increase in normal stress. The interface peak shear displacement decreased with the increase in normal stress. Under different water content conditions, the peak shear stress decreases with increasing water content, while the corresponding peak shear displacement increases. The internal friction angle and adhesion at the pile–soil interface decreased rapidly and exponentially with increasing water content of the soil around the pile. The interfacial adhesion varies in the range of 1.07–13.76 kPa and the internal friction angle in the range of 1.8–6.1°. The change in water content when the water content of marine soft clay is less than the liquid limit has a great influence on the interface shear strength. The peak shear stress increases with increasing resting time, while the corresponding peak shear displacement decreases for different resting times. The Internal friction angle and adhesion at the pile–soil interface increases exponentially with the resting time. Interfacial adhesion changes in the range of 1.8–4.9 kP, and the internal friction angle is 2.8–4.7°. The strength of the pile–soil interface grows with the advancement of the resting time, and the bearing performance of the jacked pile is improved, with the most significant effect in 14 days. Based on multiple linear regression analyses, the effects of normal stress and water content on interfacial shear strength are comparable and the effect of normal stress on the shear strength is more significant compared with the resting time. The test results provide valuable reference for the design and construction of jacked piles in marine soft ground. Full article

This paper presents a multiple step-stress accelerated life test using type II censoring. Assuming that the lifetimes of the test item follow the gamma distribution, the maximum likelihood estimation and Bayesian approaches are used to estimate the distribution parameters. In the Bayesian approach, new parametrizations can lead to new prior distributions and can be a useful technique to improve the efficiency and effectiveness of Bayesian modeling, particularly when dealing with complex or high-dimensional models. Therefore, in this paper, we present two sets of prior distributions for the parameters of the accelerated test where one of them is based on the reparametrization of the other. The performance of the proposed prior distributions and maximum likelihood approach are investigated and compared by examining the summaries and frequentist coverage probabilities of intervals. We introduce the Markov Chain Monte Carlo (MCMC) algorithms to generate samples from the posterior distributions in order to evaluate the estimators and intervals. Numerical simulations are conducted to examine the approach’s performance and one-sample lifetime data are presented to illustrate the proposed methodology. Full article

Before calcification begins, the early embryonic and fetal skeletal development of both mammalian Homo sapiens and the chondrichthyan fish Raja asterias consists exclusively of cartilage. This cartilage is formed and shaped through processes involving tissue segmentation and the frequency, distribution, and orientation of chondrocyte mitoses. In the subsequent developmental phase, mineral deposition in the cartilage matrix conditions the development further. The stiffness and structural layout of the mineralized cartilage have a significant impact on the shape of the anlagen (early formative structure of a tissue, a scaffold on which the new bone is formed) and the mechanical properties of the skeletal segments. The fundamental difference between the two studied species lies in how calcified cartilage serves as a scaffold for osteoblasts to deposit bone matrix, which is then remodeled. In contrast, chondrichthyans retain the calcified cartilage as the definitive skeletal structure. This study documents the distinct mineral deposition pattern in the cartilage of the chondrichthyan R. asterias, in which calcification progresses with the formation of focal calcification nuclei or “tesserae”. These are arranged on the flat surface of the endo-skeleton (crustal pattern) or aligned in columns (catenated pattern) in the radials of the appendicular skeleton. This anatomical structure is well adapted to meet the mechanical requirements of locomotion in the water column. Conversely, in terrestrial mammals, endochondral ossification (associated with the remodeling of the calcified matrix) provides limb bones with the necessary stiffness to withstand the strong bending and twisting stresses of terrestrial locomotion. In this study, radiographs of marine mammals (reproduced from previously published studies) document how the endochondral ossification in dolphin flippers adapts to the mechanical demands of aquatic locomotion. This adaptation includes the reduction in the length of the stylopodium and zeugopodium and an increase in the number of elements in the autopodium’s central rays. Full article

Bacterial microbiomes influence global carbon and nutrient cycling as the environment changes. Rain-fed rock basins are ephemeral aquatic systems, potentially subject to extreme environmental stress, that can host a wide variety of biological communities, including bacteria. However, bacterial communities are barely described in these habitats. Here we provide a detailed description on the occurrence, diversity and distribution patterns of the bacterial communities within and between rain-fed granite mountain rock basins located in the Sierra de Guadarrama National Park, Spain, using high-throughput sequencing of 16S RNA. We recovered a highly diverse community consisting of 3174 operational taxonomic units (OTUs) belonging to 32 phyla. In total, 50% of OTUs were shared among basins and 6–10% were basin-exclusive OTUs, suggesting a robust global bacterial metacommunity colonizes the basins. The existence of 6% replicate-exclusive OTUs and the fact that at least four replicates were required to catalogue 90% of the basin bacterial community emphasized the heterogeneity of these habitats. Both environmental filtering and random dispersal are likely to be involved in the arrangement of the bacterial communities. The taxa identified in this study are versatile in metabolism, and some have biotechnological potential. The taxonomic affiliation of many of the OTUs found suggests that rain-fed rock basins could be a resource for mining novel bacterial biocompounds. Full article

As a type of magnetic sensor known for its high reliability and long lifespan, the reliability issues of Hall current sensors have attracted attention in fields such as electromagnetic compatibility. However, there is still a lack of sufficient failure data for reliability prediction. Therefore, a small-sample reliability prediction method based on the improved Bayes method is proposed. Firstly, the pseudo-failure lifespan data are acquired through the accelerated degradation testing of Hall current sensors subjected to temperature and humidity stressors, and the life is examined by the Weibull distribution; then, the data expanded using the BP neural network model are used as the a priori information, and the parameter estimation of the Weibull distribution is obtained by the Bootstrap method and Gibbs sampling; finally, the Peck accelerated model is implemented to achieve the normal temperature-humidity reliability prediction of Hall current sensors under stress, and the utility of the enhanced Bayes technique is confirmed through the application of the Wiener stochastic process model. Full article

Total ankle arthroplasty (TAA) is a motion-preserving treatment for end-stage ankle arthritis. An effective tool for analyzing these implants’ mechanical performance and longevity in silico is finite element analysis (FEA). An FEA in ABAQUS was used to statically analyze the mechanical behavior of the ultra-high-molecular-weight polyethylene (UHMWPE) bearing component at varying dorsiflexion/plantarflexion ankle angles and axial loading conditions during the stance phase of the gait cycle for a single cycle. The von Mises stress and contact pressure were examined on the articulating surface of the bearing component in two newly installed fixed-bearing TAA implants (Wright Medical INBONE II and Exactech Vantage). Six different FEA models of variable ankle compressive load levels and ankle angle positions, for the varying subphases of the stance phase of the gait cycle, were created. The components in these models were constrained to be conducive to the bone–implant interface, where implant loosening occurs. Our results showed that the von Mises stress and contact pressure distributions increased as the compressive load increased. The highest stress was noted at dorsiflexion angles > 15°, in areas where the UHMWPE liner was thinnest, at the edges of the talar and UHMWPE components, and during the terminal stance phase of the gait cycle. This static structural analysis highlighted these failure regions are susceptible to yielding and wear and indicated stress magnitudes that are in agreement (within 25%) with those in previous static structural TAA FEAs. The mechanical wear of the UHMWPE bearing component in TAA can lead to aseptic loosening and peri-implant cyst formation over time, requiring surgical revision. This study provides ankle replacement manufacturers and orthopedic surgeons with a better understanding of the stress response and contact pressure sustained by TAA implants, which is critical to optimizing implant longevity and improving patient care. Full article

Previous research has highlighted significant phenotypic discrepancies between knockout and knockdown approaches in zebrafish, raising concerns about the reliability of these methods. However, our study suggests that these differences are not as pronounced as was once believed. By carefully examining the roles of maternal and zygotic gene contributions, we demonstrate that these factors significantly influence phenotypic outcomes, often accounting for the observed discrepancies. Our findings emphasize that morpholinos, despite their potential off-target effects, can be effective tools when used with rigorous controls. We introduce the concept of graded maternal contribution, which explains how the uneven distribution of maternal mRNA and proteins during gametogenesis impacts phenotypic variability. Our research categorizes genes into three types—susceptible, immune, and “Schrödinger” (conditional)—based on their phenotypic expression and interaction with genetic compensation mechanisms. This distinction provides new insights into the paradoxical outcomes observed in genetic studies. Ultimately, our work underscores the importance of considering both maternal and zygotic contributions, alongside rigorous experimental controls, to accurately interpret gene function and the mechanisms underlying disease. This study advocates for the continued use of morpholinos in conjunction with advanced genetic tools like CRISPR/Cas9, stressing the need for a meticulous experimental design to optimize the utility of zebrafish in genetic research and therapeutic development. Full article