Search Results (239,861)

Herbicides are the most widely used agrochemicals in crop protection, which has led to serious environmental pollution around the world, including soil ecosystems. It is important to look for new solutions that lead to an improvement in soil quality, even if only through the use of hydrogels. The aim of this study was therefore to determine the effect of sodium alginate on the microbiological and biochemical properties of sulcotrione-treated soil. It was found that both the herbicide and the sodium alginate had a significant effect on the soil environment. An amount of 10 g kg⁻¹ of sodium alginate was applied to the soil, while sulcotrione was applied to the soil in the following amounts: 0.00 (C), 0.200 (R), 0.999 (5R), and 9.999 mg kg⁻¹ (50R). Sulcotrione stimulated the activity of dehydrogenases, catalase, arylsulfatase, and β-glucosidase and inhibited the activities of alkaline phosphatase, acid phosphatase, and urease as well as the proliferation of organotrophic bacteria, actinobacteria, and fungi. This caused an increase in the colony development index (CD) of organotrophic bacteria and fungi and decreased the colony development index value of actinobacteria. It also increased the value of the ecophysiological diversity index (EP) of fungi. The addition of sodium alginate to the soil increased the numbers of organotrophic bacteria, actinobacteria, and fungi as well as the activities of dehydrogenases, catalase, urease, alkaline phosphatase, and arylsulfatase. The hydrogel had different effects on β-glucosidase activity. Acid phosphatase showed a significant decrease in activity after the addition of sodium alginate to the soil. Under the influence of sodium alginate, there was an increase in the index of colony development of actinobacteria and fungi, while there were decreases in organotrophic bacteria and the index of ecophysiological diversity of actinobacteria and fungi. The proliferation of microorganisms and the enzymatic activity of the soil changed over time both in soil enriched with sodium alginate and without its addition. This study may be useful for evaluating the effects of sulcotrione on the microbiological and biochemical properties of soil and the effectiveness of sodium alginate in improving the quality of soil exposed to sulcotrione. Full article

The growing variety of transportation options and increasing traffic congestion pose new challenges for road safety. As a result, there is an intensified focus on developing automated driving features and assistance systems aimed at minimizing accidents caused by human errors. The creation of these systems requires a substantial amount of testing kilometers, with estimates suggesting that around 2.1 billion kilometers would be necessary to ensure that each situation pertinent to the driving function is encountered at least once with a probability of 50%. This paper advances the microscopic simulation of traffic scenarios beyond linear patterns, utilizing the open-source environment openPASS. It addresses the research question of whether existing microscopic simulations are able to realistically represent non-linear traffic scenarios. A comprehensive toolchain integrates simulation with video recordings and laser scans. The study compares recorded traffic flow data with simulations at a T-junction, assessing the realism of vehicle models and trajectory representation. Three scenarios are analyzed, considering vehicles and pedestrians. The 3D geometry of the scene was captured with a laser scanner, enabling the mapping of recorded video data onto a geo-referenced environment. Object trajectories were extracted using an ’Regions with Convolutional Neural Networks features’ object detector. While openPASS simulated vehicle and pedestrian behaviors effectively, limitations in trajectory variability and reaction times were observed. These findings highlight the need for more realistic behavior models. This research emphasizes the necessity for improvements to accommodate complex driving behaviors and pedestrian dynamics. Full article

The probiotic bacterium Bifidobacterium animalis subsp. lactis BB-12 (BB-12) was encapsulated in two composites, alginate/agar and alginate/agar/casein. The network structure and physicochemical properties of these composites are influenced by complex interactions, including hydrogen bonding, electrostatic forces between biopolymers, calcium ions, and the encapsulated bacteria. The composites demonstrated a granular surface, with the granules being spatially oriented on the alginate/agar/BB-12 surface and linearly oriented on the alginate/agar/casein/BB-12 surface. They possess a highly organized microparticle structure and exhibit viscoelastic solid-like behavior. The alginate/agar/BB-12 composite showed higher storage modulus, shear stress, and shear strain values, indicating enhanced stability in various physical environments. Both composites displayed good thermal stability, aligning with their rheological properties, confirming their well-ordered structures. Despite differences in composite structures, the release mechanism of bacteria is governed by Fickian diffusion through the composite matrix. Based on physicochemical properties, the alginate/agar/casein composite is recommended for dairy product fermentation, while the alginate/agar composite seems more suitable for oral use. These findings provide new insights into the interactions between bacterial cultures and alginate composite ingredients. Full article

Prostate cancer (PCa) is a common cancer among men, and it has a multifactorial etiology. Cadmium (Cd), a toxic heavy metal classified as a carcinogen by the IARC, can cause various acute and chronic effects. This systematic review and meta-analysis aims to update previous findings on the association between Cd exposure and PCa. We carried out a literature search in PubMed, Web of Science, and Scopus up to May 2024, identifying eight new articles. The effect size from the highest and lowest exposure categories were extracted and analyzed using a random-effects model. Heterogeneity was assessed with the I² test, and publication bias was evaluated using funnel plot asymmetry. Overall, the effect size for PCa associated with Cd exposure, combining previous and new articles, was 1.11 (95% CI 0.85–1.45). Heterogeneity was high, but no significant publication bias was detected. For studies from Europe, the effect size was 1.47 (95% CI 1.00–2.17). Notably, 11 articles from the previous systematic review and meta-analysis highlighted that higher Cd exposure is significantly associated with more aggressive histopathological types of PCa (OR 1.50, 95% CI 1.08–2.07). These findings suggest a potential public health concern, indicating the need for further research to clarify the risk associated with Cd exposure. Full article

The main objective of a machine learning (ML) system is to obtain a trained model from input data in such a way that it allows predictions to be made on new i.i.d. (Independently and Identically Distributed) data with the lowest possible error. However, how can we assess whether the training and test data have a similar distribution? To answer this question, this paper presents a proposal to determine the degree of distribution shift of two datasets. To this end, a metric for evaluating complexity in datasets is used, which can be applied in multi-class problems, comparing each pair of classes of the two sets. The proposed methodology has been applied to three well-known datasets: MNIST, CIFAR-10 and CIFAR-100, together with corrupted versions of these. Through this methodology, it is possible to evaluate which types of modification have a greater impact on the generalization of the models without the need to train multiple models multiple times, also allowing us to determine which classes are more affected by corruption. Full article

Nanomaterials are attractive materials for researchers because they have essential characteristics in terms of their properties. Carbon has an ample range of crystalline allotropes. Some, such as graphite and diamond, have been known since ancient times, while new forms of carbon with potential for various applications have been discovered in recent decades. Since the discovery of graphene 20 years ago, research has increased on composite materials that take advantage of carbon structures for their electrical, thermal, and mechanical properties and their ability to be synthesized at the nanometer scale. Graphene has stood out above other nanomaterials due to its surprising properties and high impact on technological research, so its uses have diversified in different areas of science such as medicine, electronics, engineering, etc. This work aims to show some new and innovative applications of graphene, on which we can see its versatility as engineering material. It also seeks to show its potential in research and development processes for its use. These are key components of advanced graphene-based materials systems under active development, with an eye on the future of advanced materials science and technology. Full article

“Bluebeard” (ATU 321: Maiden-Killer), a fairy tale about a wealthy noble man and serial killer, is the most gruesome of Charles Perrault’s fairy tales. Bluebeard epitomizes evil and horror. In Perrault’s tale, Bluebeard’s evilness is linked to patriarchy and power, as symbolized by the villain’s iconic blue beard. Historically linked to Henry VIII (1491–1547), King of England, Bluebeard has also been associated with Breton commander Gilles de Rais who was hanged for sorcery and satanic abuse. This article examines how contemporary francophone “Bluebeard” variants refashion and redefine evil and whether they contain any new morals linked to evilness. Do they depict Bluebeard as a satanic, intrinsic force of evil or do they portray him in a less Manichean manner, as contemporary tales tend to do with monsters? Starting with Perrault’s famous tale, this article reveals how Bluebeard, the evil mass murderer figure and polygamist, is recast in a variety of contemporary francophone texts from Morocco, Belgium and France, with retellings by Michel Tournier (1981), Marie Darrieussecq (2002), La Barbe Bleue (Bluebeard) (2009), Amélie Nothomb (2012), Tahar Ben Jelloun (2014), Jacqueline Kelen (2014), and Cécile Coulon (2015). These modern variants illustrate Elliott Oring’s ideas about comparison and cultural context (see Oring 1986). A discussion of various French contemporary versions with a special emphasis of Ben Jelloun’s Moroccan retelling of “Bluebeard” open avenues for cross-cultural dialogue, highlighting how this tale evolves to fit different cultural contexts and continues to resonate today. Full article

Metastatic colorectal cancer is a leading cause of cancer-related death across the world. The treatment paradigm has shifted away from systemic chemotherapy alone to include targeted therapy and immunotherapy. The past two decades have been characterized by increased investigation into molecular profiling of colorectal cancer. These molecular profiles help physicians to better understand colorectal cancer biology among patients with metastatic disease. Additionally, improved data on genetic pathways allow for specific therapies to be targeted at the underlying molecular profile. Investigation of the EGFR, VEGF, HER2, and other pathways, as well as deficient mismatch repair, has led to the development of multiple targeted therapies that are now utilized in the National Comprehensive Cancer Network guidelines for colon and rectal cancer. While these new therapies have contributed to improved survival for metastatic colorectal cancer, long-term survival remains poor. Additional investigation to understand resistance to targeted therapy and development of new targeted therapy is necessary. New therapies are under development and are being tested in the preclinical and clinical settings. The aim of this review is to provide a comprehensive evaluation of molecular profiling, currently available therapies, and ongoing obstacles in the field of colorectal cancer. Full article

Singlet fission (SF) is a photophysical process where one singlet exciton splits into two triplet excitons. To construct design guidelines for engineering directional triplet exciton migration, we investigated the SF dynamics in symmetric linear heterotrimer systems consisting of different unsubstituted or 6,13-disubstituted pentacene derivatives denoted as X/Y (X, Y: terminal and center monomer species). Time-dependent density functional theory (TDDFT) calculations clarified that the induction effects of the substituents, represented as Hammett’s para-substitution coefficients σp, correlated with both the excitation energies of S₁ and T₁ states, in addition to the energies of the highest occupied and lowest unoccupied molecular orbitals (HOMO and LUMO). Electronic coupling calculations and quantum dynamics simulations revealed that the selectivity of spatially separated TT states for heterotrimers increased over 70%, superior to that in the homotrimer: an optimal region of the difference in σp between the substituents of X and Y for the increase in SF rate was found. The origin of the rise in SF rate is explained by considering the quantum interference effect: reduction in structural symmetry opens new interaction paths, allowing the S₁-TT mixing, which contributes to accelerating the hetero-fission between the terminal and center molecules. Full article

A wind erosion instrument is a core instrument for collecting sand particles in wind and sand flows and studying the laws of wind and sand movement. To study the influence of the internal structure of the wind erosion instrument on its sand collection efficiency, a built-in louver separation device was designed. Based on CFD and Fluent 2022 software, numerical analysis was conducted using an RNG k-ε model, and the discrete phase model (DPM) method was used to calculate the sand collection efficiency. The flow field analysis of the new wind–sand separator was carried out. The influence of blade inclination angle, blade thickness, and blade number on sand collection efficiency was studied using single-factor and response surface analysis methods. The optimal parameter combination was obtained as blade inclination angle of 30°, blade thickness of 1.25 mm, and blade number of 10. A simulation model was established based on the optimal combination parameters, and the performance of the wind erosion instrument before and after the addition of the louver separation device was compared. The simulation results show that adding a louver separation device can increase static pressure, alleviate short-circuit flow and back-mixing phenomena, and stabilize the flow field; increasing tangential velocity leads to an increase in particle centrifugal force; reduce axial velocity, prolong particle stagnation time, and minimize particle escape. The particle trajectory pattern is mostly a continuous spiral path, which is conducive to capturing particles and improving sand collection efficiency. Compared with the original structure, for particles with diameters ranging from 0.001–0.05 mm, 0.005–0.01 mm, 0.01–0.05 mm, 0.05–0.1 mm, and 0.1–0.5 mm, the addition of a louver separation device increased the sand collection efficiency by 32.74%, 22.55%, 33.17%, 11.45%, and 0.13%, respectively. When the wind speed is 13.8 m/s and the diameter range is 0.001–0.5 mm, the average sand collection efficiency obtained from simulation tests and wind tunnel tests is 86.18% and 84.32%, respectively, with an error of 2.2%. The simulation results are reliable. The research results show that adding a louver separation device can improve the sand collection efficiency of the wind erosion instrument, and has better overall performance compared to the original wind–sand separator. This study provides a basis for further research on the structure of wind erosion gauges and the environmental protection of farmland. Strengthening land management can effectively protect soil resources, reduce wind erosion, ensure the stability of the ecosystem, and lay the foundation for promoting the sustainable use of land. Full article

In an attempt to explore the RNA viromes of two German rivers, we searched the virus particle contents of one 50 L water sample each from the Teltow Canal and the Havel River for viruses assumed to infect invertebrates. More than 330 complete and partial virus genomes up to a length of 37 kb were identified, with noda-like and reo-like viruses being most abundant, followed by bunya-like and birna-like viruses. Viruses related to the Permutotetraviridae, Nidovirales, Flaviviridae, Rhabdoviridae and Chuviridae as well as the unclassified Jῑngmén virus and Negev virus groups were also present. The results indicate a broad extent of recombinant virus genomes, supporting the concept of the modularity of eukaryotic viruses. For example, novel combinations of genes encoding replicase and structural proteins with a jellyroll fold have been observed. Less than 35 viruses could be assigned to existing virus genera. These are (i) an avian deltacoronavirus which was represented by only one short contig, albeit with 98% similarity, (ii) a seadornavirus and a rotavirus, and (iii) some 30 nodaviruses. All remaining viruses are novel and too diverse for accommodation in existing genera. Many of the virus genomes exhibit ORFans encoding hypothetical proteins of up to 2000 amino acids without conserved protein domains. Full article

Chronic wounds are often caused or exacerbated by microbial biofilms that are highly resistant to antimicrobial treatments and that prevent healing. This study compared the antimicrobial and antibiofilm activity of nine topical wound treatments, comprising gels with different concentrations of poloxamer 407 (20–26%) and different pH levels (4–6) and containing polyhexanide (PHMB) as an antimicrobial agent; the effects of pH on wound gels containing this agent have not been previously reported. The wound gel formulations were tested against six common wound-associated microbial pathogens: Staphylococcus aureus, S. epidermidis, Pseudomonas aeruginosa, Escherichia coli, Acinetobacter baumannii, and Candida albicans. Time-kill assays were used to assess antimicrobial activity against planktonic forms of each species, and a colony biofilm model was used to assess antibiofilm activity against existing biofilms as well as inhibition of new biofilm formation. Biofilm inhibition activity was also assessed in the presence of common wound dressing materials. Wound gels with higher pH levels exhibited stronger antimicrobial activity, while poloxamer 407 concentrations >20% negatively impacted antimicrobial activity. Wound gel formulations were identified that had antimicrobial, antibiofilm, and biofilm inhibition activity against all tested species in vitro. Biofilm inhibition activity was not affected by contact with common wound dressings. Further development of these wound gels may provide a valuable new option for the treatment and prevention of chronic wounds. Full article

Secreted phospholipase 2 (sPLA2) is the largest family of phospholipase A2 (PLA2) enzymes with 11 mammalian isoforms. Each sPLA2 exhibits different localizations and specific properties, being involved in a very wide spectrum of biological processes. The enzymatic activity of sPLA2 has been well described; however, recent findings have shown that they could regulate different signaling pathways by acting directly as ligands. Arachidonic acid (AA) and its derivatives are produced by sPLA2 in collaboration with other molecules in the extracellular space, making important impacts on the cellular environment, being especially relevant in the contexts of immunity and cancer. For these reasons, this review focuses on sPLA2 functions in processes such as the promotion of EMT, angiogenesis, and immunomodulation in the context of tumor initiation and progression. Finally, we will also describe how this knowledge has been applied in the search for new sPLA2 inhibitory compounds that can be used for cancer treatment. Full article

Magnesium phosphate cement (MPC), a cementitious material that hardens through an acid–base reaction, is theoretically expected to exhibit strong acid resistance. However, studies on the durability of MPC in acidic environments remain limited. This study aims to systematically evaluate the acid resistance of MPC in common inorganic acid solutions across various pH levels. By measuring changes in compressive strength, mass loss, apparent changes, pH changes, and the microstructure evolution of MPC under acidic conditions, the mechanisms and influencing factors of its acid resistance are revealed. The results indicate that at pH levels of 1.0 and 2.0, MPC’s resistance to H₂SO₄ and HCl erosion is markedly superior compared to its performance against H₃PO₄, as evidenced by compressive strength retention, mass loss, and visible erosion. At pH levels above 2.0, MPC demonstrates robust resistance to all tested corrosive media, with compressive strength retention ranging from 68.9% to 86.9%, irrespective of the acid source. Although new corrosion products form in these acidic environments, the adverse effects of NH₄/P loss from struvite, along with the redissolution of corrosion products due to their higher solubility, increase porosity and subsequently reduce the mechanical strength. Nevertheless, considering that strength retention is significantly higher than that of other cement-based materials reported in the literature, MPC still exhibits good acid resistance and is suitable for environments requiring enhanced resistance to acid corrosion. Full article

Vascular calcification (VC) is a complex process involving vascular smooth muscle cell (VSMC) osteogenic differentiation, inflammation, and extracellular vesicle (EV) calcification and communication networks. Gla rich protein (GRP) is a calcification inhibitor involved in most of these processes. However, the molecular mechanism of GRP in VC and the specific characteristics, cargo, and functionality of calcifying EVs require further elucidation. Here, we use a combination of human ex vivo aortic fragments and primary vascular smooth muscle cell (VSMC) models to obtain new information on GRP function in VC and EVs released by VSMCs. We demonstrate that GRP inhibits VSMC osteogenic differentiation through downregulation of bone-related proteins and upregulation of mineralization inhibitors, with decreased mineral crystallinity in EVs deposited into the tissue extracellular matrix (ECM). EVs isolated by ultracentrifugation at 30K and 100K from the cell media (CM) and deposited in the ECM from control (CTR) and mineralizing (MM) VSMCs were biochemically, physically, and proteomically characterized. Four different EV populations were identified with shared markers commonly present in all EVs but with unique protein cargo and specific molecular profiles. Comparative proteomics identified several regulated proteins specifically loaded into MM EV populations associated with multiple processes involved in VC. Functional analysis demonstrated that 30K and 100K ECM-MM EVs with higher calcium and lower GRP levels induced macrophage inflammation. Our findings reinforce the functional relevance of GRP in multiple VC processes and suggest that ECM EVs released under calcification stress function as a new signaling axis on the calcification–inflammation cycle. Full article