Search Results (15,383)

This study investigated the effect of natural compounds from Nile tilapia (Oreochromis niloticus) skin on wound healing in IL-10 knockout mice. The healing fraction, Fraction T19, was obtained through hydrolysis with trypsin. In vitro, T19 was not cytotoxic to RAW 264.7 macrophage cells, promoting increased cell proliferation and migration. In vivo, mice (n = 30) were divided into three groups with 12 mm wounds in the dorsal region: control (distilled water), T1 (T19 at 125 μg/mL), and T2 (T19 at 250 μg/mL). Daily applications were performed, with tissue removal after nine days. The results showed that T19 increased the production of nitric oxide (NO) and hydrogen peroxide (H₂O₂), preventing wound contamination. There was an increase in pro-inflammatory (IL-2 and IFNγ) and anti-inflammatory (IL-4) cytokines, as well as cell proliferation markers (PCNA and KI67). Antibodies CD31, CD163, and COX-2 indicated an increase in the formation of new vessels and a reduction in inflammation. Both groups treated with T19 showed better healing results, with better effects observed at higher doses. It was concluded that T19 can effectively modulate the skin repair process and represent an alternative therapeutic for improving the quality of wound skin, especially in the clinical context. Formulations using tilapia skin are safe and effective for accelerating wound healing. Full article

Modern aquaculture utilizes computer vision technology to analyze underwater images of fish, contributing to optimized water quality and improved production efficiency. The purpose of this study is to efficiently perform underwater fish detection and tracking using multi-object tracking (MOT) technology. To achieve this, the FairMOT model was employed to simultaneously implement pixel-level object detection and re-identification (Re-ID) functions, comparing two backbone models: FairMOT+YOLOv5s and FairMOT+DLA-34. The study constructed a dataset targeting the popular black porgy in Korean aquaculture, using underwater video data from five different environments collected from the internet. During the training process, the FairMOT+YOLOv5s model rapidly reduced train loss and demonstrated stable performance. The FairMOT+DLA-34 model showed better results in ID tracking performance, with an accuracy of 44.1%, an IDF1 of 11.0%, an MOTP of 0.393, and an IDSW of 1. In contrast, the FairMOT+YOLOv5s model recorded an accuracy of 43.8%, an IDF1 of 14.6%, an MOTP of 0.400, and an IDSW of 10. The results of this study indicate that the FairMOT+YOLOv5s model demonstrated higher IDF1 and MOTP scores compared to the FairMOT+DLA-34 model, while the FairMOT+DLA-34 model showed superior performance in ID tracking accuracy and had fewer ID switches. Full article

Sulfidic caves support diverse and abundant subterranean communities, including numerous endemic species and complex food webs, though the full extent of species diversity and resource utilization in these ecosystems remains largely unexplored. This paper presents the results of biological surveys conducted from 2023 to 2024 in Sulfur Cave, located in the Vromoner Canyon on the Greek–Albanian border, focusing on microbial, vertebrate, and invertebrate communities and investigating the structure of the subterranean food web. The microbial communities from the different biofilms are dominated by chemosynthetic sulfur-oxidizing microorganisms, specifically filamentous bacteria such as Thiotrix and Beggiatoa. Two species of fish, an eel (Anguilla sp.) and a Cyprinid (Alburnoides sp.), and six bat species from three families (Rhinolophidae, Miniopteridae, and Vespertilionidae) were documented. The invertebrate fauna includes five aquatic species, 25 terrestrial species, and four amphibiotic species. Among these, eight species are endemic, and seven species exhibit troglomorphic traits. Stable isotope analysis showed light carbon and nitrogen values for the terrestrial and aquatic invertebrates, suggesting that subterranean communities rely on food produced in situ by chemoautotrophic microorganisms. Our results identified cave areas of significant biological relevance and provided reference data to inform conservation actions aimed at preserving the biodiversity of this sulfidic cave. Full article

Scomber japonicus is widely distributed off the coast of Japan and in the northwestern Pacific. It is an important target for fisheries. To reveal the differences in diet shifts and niche changes of S. japonicus, we collected samples in the high seas of the northwest Pacific (38°59′ N–43°00′ N, 150°30′ E–161°48′ E) from June to August 2021. We utilized stable isotope and fatty acid analyses to study the differences in body length and sex of S. japonicus. The results showed no significant differences in stable isotope values and fatty acid composition between male and female individuals (p > 0.05). Differences in δ¹³C and δ¹⁵N values among different body length groups were also not significant (p > 0.05). Both δ¹³C and δ¹⁵N values showed a trend of increasing and then decreasing across different body length groups. The niche width of S. japonicus in different body length groups first increased and then decreased, with niche overlap among the groups exceeding 60%. Principal component analysis (PCA) results showed that the main fatty acids in S. japonicus were C14:0, C16:0, C18:0, C16:1n-7, C18:1n-9, C18:3n-6, C20:5n-3, C20:4n-6 and 22:6n-3. Except for C18:0 and C20:4n-6, the content of the other fatty acids showed significant differences among different body length groups (p < 0.05). The results of the similarity analysis (ANOSIM) indicated that the fatty acid compositions of the 100–130 mm length group were significantly different from those of the 131–160 mm and 161–190 mm length groups (p < 0.05). However, there were no significant differences among the other size groups (p > 0.05). During the growth and development of S. japonicus, the proportion of krill in their diet gradually decreased. Meanwhile, their consumption of zooplankton, diatoms and fish significantly increased. Additionally, S. japonicus also consumed crustaceans, but their intakes of planktonic bacteria and green algae were relatively low. We suggested that there were no significant differences between male and female individuals of S. japonicus. As they grew and developed, the ecological niche and feeding habits of S. japonicus continuously changed. Full article

In order to understand the community structure of plankton and environmental factors in the Chongqing section of the National Nature Reserve for Rare and Endemic Fishes (referred to as the “Reserve”) along the upper Yangtze River, this study investigated phytoplankton and zooplankton in the water body from 2021 to 2022. The results revealed a diverse phytoplankton community with 243 species from 105 genera and 8 phyla, dominated by Bacillariophyta and Chlorophyta. Phytoplankton showed average densities of 1.7 × 10⁶ cells/L and biomass of 2.6221 mg/L, following a seasonal pattern of summer > spring > winter. Zooplankton analysis identified 141 species from 77 genera and 4 phyla, with rotifers most abundant, followed by protozoa. Zooplankton displayed average densities of 0.17 × 10⁴ ind./L and biomass of 0.3226 mg/L, also following a seasonal pattern of summer > spring > winter. Total phosphorus (TP) emerged as the primary environmental factor influencing plankton community structure, positively correlating with phytoplankton density and zooplankton biomass. Plankton biodiversity indices classified water quality in the Chongqing section of the “Reserve” as oligo-/mesotrophic. Overall, plankton diversity in this section is notably rich, with similar species composition between mainstems and tributaries but seasonal variations in community structure. While mainstem water quality generally meets standards, some tributaries exhibit varying degrees of pollution, underscoring the need for improved ecological management and protection measures. This is crucial for maintaining the sustainability of the ecosystem. Full article

The Qilu Lake is an eutrophic shallow lake located in Yunnan Province, China. An ecological fishery project was initiated in the lake from 2011 to 2021 to introduce filter-feeding fish species that feed on algae, with the aim of improving water quality. In January 2022, when the ecological fishery project expired, all fisheries-related activities (including fish stocking and fishing activities) ceased in the lake. To comprehensively evaluate the initial alterations in fish community structure and diversity resulting from the fishing cessation in the Qilu Lake, the present study conducted field surveys within the one year before the fishing cessation (referred to as BFC) and the one year after the fishing cessation (referred to as AFC). A total of twenty-one fish species were collected, including four native species. Four species were recorded in the lake for the first time, including Pelteobagrus fulvidraco, Pelteobagrus vachelli, Paramisgurnus dabryanus, and Hyporhamphus intermedius. The number of fish species decreased from 21 to 13 following the fishing cessation. The fishes collected in both BFC and AFC are mainly omnivorous-feeding and of bottom-dwelling habits. The mean size of the fishes in the AFC sample shows a significant decrease compared to those in BFC. After the fishing cessation, the Shannon–Wiener diversity index and Margalef richness index of the fish slightly declined. The fish community structure of the Qilu Lake exhibits a high degree of similarity to adjacent lakes in central Yunnan. Our study demonstrates a significant shift in the fish community of the Qilu Lake following the fishing cessation, one which may adversely impact the stability of the lake ecosystem. To enhance fish species diversity in the Qilu Lake, it is recommended that policies be implemented to promote the ecological fishery project and improve habitat restoration for native fish species, while also regulating fish community structure. Full article

The present research examined the impact of L-glutamic acid (Glu) supplementation on the growth performance, muscle composition, gene expression correlated with muscle growth, and intestinal health of largemouth bass. There were 525 fish in total, which were distributed randomly into five groups. Each group had three replicates, and each replicate consisted of 35 fish. Groups with control and experimental diets were assigned glutamic acid amounts of 0.2%, 0.4%, 0.6%, and 0.8%. The findings demonstrated that glutamic acid supplementation enhanced growth performance, feed intake (FI), and condition factor (CF), with the best value being attained at 0.4% Glu. The mean muscle fiber area was increased and the muscle fiber density was decreased in the 0.6% Glu group. The levels of total amino acids and specific amino acids, such as glutamic acid, aspartic acid, leucine, valine, alanine, and glycine, were shown to be higher in the 0.6% Glu group. In the 0.6% Glu group, the mRNA expression levels of atrogin-1, murf-1, foxo3a, and 4e-bp1 were decreased compared to the control group. Conversely, the mRNA expression levels of myf5, myog, myod, s6k1, tor, akt, and pi3k were increased in the 0.6% Glu group compared to the control group. The 0.4% Glu group had higher intestinal amylase, lipase, and protease activities and greater villus height, villus width, and muscle thickness. In summary, Glu can support largemouth bass growth, muscular development, intestinal digestion, and absorption. Full article

In the marine environment, fish parasites are present in most seafood species. The most common are nematodes of the genus Anisakis, which can parasitize human tissues, causing anisakiasis and allergies—in some cases with a strong reaction, such as anaphylactic shock. This happens when people ingest live or dead larvae present in the muscles or viscera of a wide range of fish and cephalopods. Consumer education has been positioned as one of the most effective alternatives for its prevention. This study, carried out in Asturias (northwest Spain), sought to identify the seafood products that present the greatest risk of anisakiasis for consumers, taking into account their consumption, the prevalence of Anisakis, and consumer knowledge about this parasitosis. In the results, hake (Merluccius merluccius) and cod (Gadus morhua), frequently consumed in the region and with high parasite prevalence, do not pose a great risk because they are consumed when well cooked. Instead, sardine (Sardina pilchardus), highly consumed and less parasitized, and anchovy (Engraulis encrasicolus), highly parasitized and less consumed, would exhibit a medium risk. Young participants know more about the risks of anisakiasis from raw seafood. The gaps detected in the knowledge about the ability of temperature treatments to eliminate parasites, especially in allergic people, must be addressed for better prevention. We suggest campaigns adapted to the population sectors. Full article

The microsomal metabolism of phenol (11 °C) over an annual reproductive cycle from June to December was studied using fall spawning adult brook trout (Salvelinus fontinalis). Hepatic microsomes were isolated from three male and three female fish each month. Incubations were optimized for time, cofactor concentration, pH, and microsomal protein concentration. The formation of phase I ring-hydroxylation metabolites, i.e., hydroquinone (HQ) and catechol (CAT), was quantified by HPLC with dual-channel electrochemical detection. Sample preparation and chromatographic conditions were optimized to achieve the separation and sensitivity required for the analysis of these labile products. Biotransformation of phenol over a range of substrate concentrations (1 to 150 mM) was quantified for the calculation of Michaelis–Menten constants (Km and Vmax) for each month. Results indicate a nearly equal production of HQ and CAT among males and females in late June. At the peak of maturity in October, there was an approximate ten-fold greater production of ring-hydroxylation metabolites noted in females in comparison with males on a total liver basis. In vitro phase II biotransformation of phenol glucuronidation was assessed by determining the Michaelis–Menten constants (Km, Vmax) using brook trout hepatic microsomes over a range of substrate concentrations (1 to 60 mM). Initially, there were no significant differences in the glucuronide rate of formation (pmol/min/mg protein) or total capacity (nmol/min/liver) between females and males. At the peak of maturation, the maximum rate of glucuronide formation was 4-fold less in females; however, the total capacity was 2-fold less in females due to the increased liver size in the females. The alterations in biotransformation coincided with increases in the hepatic and gonadal somatic indices and with changes in plasma hormone concentrations. These experiments provide insight into the metabolic deactivation of xenobiotics and to provide data for the prediction of altered hepatic biotransformation rates and pathways during the reproductive cycle. Full article

Three-dimensional (3D) fish hepatocyte cultures are promising alternative models for replicating in vivo data. Few studies have attempted to characterise the structure and function of fish 3D liver models and illustrate their applicability. This study aimed to further characterise a previously established spheroid model obtained from juvenile brown trout (Salmo trutta) primary hepatocytes under estrogenic stimulation. The spheroids were exposed for six days to environmentally relevant concentrations of 17α-ethinylestradiol—EE2 (1–100 ng/L). The mRNA levels of peroxisome (catalase—Cat and urate oxidase—Uox), lipid metabolism (acyl-CoA long chain synthetase 1—Acsl1, apolipoprotein AI—ApoAI, and fatty acid binding protein 1—Fabp1), and estrogen-related (estrogen receptor α—ERα, estrogen receptor β—ERβ, vitellogenin A—VtgA, zona pellucida glycoprotein 2.5—ZP2.5, and zona pellucida glycoprotein 3a.2—ZP3a.2) target genes were evaluated by quantitative real-time polymerase chain reaction. Immunohistochemistry was used to assess Vtg and ZP protein expressions. At the highest EE2 concentration, VtgA and ZP2.5 genes were significantly upregulated. The remaining target genes were not significantly altered by EE2. Vtg and ZP immunostaining was consistently increased in spheroids exposed to 50 and 100 ng/L of EE2, whereas lower EE2 levels resulted in a weaker signal. EE2 did not induce significant changes in the spheroids’ viability and morphological parameters. This study identified EE2 effects at environmentally relevant doses in trout liver spheroids, indicating its usefulness as a proxy for in vivo impacts of xenoestrogens. Full article

The combination of aquaculture industry with floating offshore wind turbines has the potential to generate significant economic advantages for both industries. To investigate this potential, the present study focuses on analyzing the heave, and pitch dynamic responses of a Spar-type floating offshore wind turbine that incorporates an aquaculture feeding system. A series of water tank model tests, together with numerical calculations, were conducted using a 1/56 scale model of a 2 MW, displacement 3500 tons, floating Spar-type wind turbine. The feeding system was placed inside the Spar and slightly above the waterline by adjusting the configuration of the total weight. The weight of the feeding system in the experiments is 100 tons, capable of sustaining 300 tons of fish for an entire week, and the realistic applications have been expanded using the numerical calculation. For this reason, the present study serves a good case study for general understanding, because the integration of the feeding system inevitably raises the center of gravity of the structure and potentially affects its overall stability. The experiments revealed no discernible increase in the heave motion. Moreover, the pitch motion theoretically increased, but occasionally decreased in the experiments with the overall inclination angles being less than 1.2 degrees during the experiments. As a result, the present study supports the practice of integrating a Spar-type wind turbine with feeding systems. Future research should continue to comprehensively examine, both experimentally and numerically, the motion responses of the wind turbine and aquaculture facilities with varying configurations. Full article

The Lower Mekong Basin has had extensive hydropower dam development, which changes its hydrologic conditions and threatens the exceptional aquatic biodiversity. This study quantifies the degree of hydrologic change between pre-impact (1965–1968) and post-impact (2018–2021) peak hydropower development in two major tributaries of the Lower Mekong Basin—the Sekong River, with the fewest dams, and the Sesan River, with the most dams. Both rivers have historically supported migratory fishes. We used daily pre- and post-impact data and the Indicators of Hydrologic Alteration framework to evaluate streamflow changes from dam development. We found significant changes in low- and high-magnitude flows in the pre- and post-impact periods of dam development. For the Sekong River, minimum flow had large fluctuations, with increases of 290% to 412% compared to the pre-impact period, while the Sesan River’s minimum flow ranged from 120% to 160% more than pre-impact. Dry season flows increased by 200 ± 63% on average in the Sekong River, which was caused by releases from upstream dams. Meanwhile, the Sesan River’s dry season flows increased by 100 ± 55% on average. This study indicates that seasonal flow changes and extreme flow events occurred more frequently in the two basins following dam construction, which may threaten the ecosystem’s function. Full article

Chronic wounds are becoming an increasing burden on healthcare services, as they have extended healing times and are susceptible to infection, with many failing to heal, which can lead ultimately to amputation. Due to the additional rise in antimicrobial resistance and emergence of difficult-to-treat Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter spp. (ESKAPE pathogens), novel treatments will soon be required asides from traditional antibiotics. Many natural substances have been identified as having the potential to aid in both preventing infection and increasing the speed of wound closure processes. Manuka honey is already in some cases used as a topical treatment in the form of ointments, which in conjunction with dressings and fish skin grafts are an existing US Food and Drug Administration-approved treatment option. These existing treatment options indicate that fatty acids from fish oil and manuka honey are well tolerated by the body, and if the active components of the treatments were better understood, they could make valuable additions to topical treatment options. This review considers two prominent natural substances with established manufacturing and global distribution—marine based fatty acids (including their metabolites) and manuka honey—their function as antimicrobials and how they can aid in wound repair, two important aspects leading to resolution of chronic wounds. Full article

During field surveys and culture procedures, large growth disparities in Anguilla japonica have been observed. However, the potential causes are unknown. This study explored differences in digestive ability, metabolic levels, and transcriptomic profiles of appetite-related genes between growth-retarded eel (GRE) and normal-growing eel (NGE) under the same rearing conditions. The results showed that growth hormone (gh) mRNA expression in GREs was considerably lower than NGEs. The levels of total protein (TP), total cholesterol (T-CHO), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), high-density lipoprotein cholesterol (HDL-C), blood ammonia (BA), blood urea nitrogen (BUN), and alkaline phosphatase (ALP) in GREs were significantly lower than in NGEs. Conversely, levels of glucose (GLU), alanine aminotransferase (ALT), and aspartate transaminase (AST) were higher in GREs. The activities of SOD, CAT, and T-AOC levels were also significantly lower in GREs, as were the activities of glucose-related enzymes including hexokinase (HK), pyruvate kinase (PK), phosphoenolpyruvate carboxykinase (PEPCK), and glucose-6-phosphatase (G6PASE). Additionally, orexigenic genes (npy and ghrelin) were dramatically downregulated, whereas anorexigenic genes (crh and pyy) were significantly upregulated in GREs. These findings suggested that variances in growth hormone, metabolic activities, and appetite level could be associated with the different growth rates of A. japonica. The present research not only revealed the characteristics of the growth, metabolism, and appetite of GREs but also offered new perspectives into the substantial growth discrepancies in A. japonica, providing novel ideas for enhancing fish growth. Full article

Fluorescence in situ hybridization (FISH) with two different probes, the canonical insect telomeric sequence (TTAGG)_n and the sequence (TTAGGGATGG)_n, was performed on meiotic chromosomes of two members of the true bug family Cimicidae (Cimicomorpha), the common bed bug Cimex lectularius Linnaeus, 1758 and the tropical bed bug C. hemipterus (Fabricius, 1803), whose telomeric motifs were not known. In both species, there were no hybridization signals with the first probe, but strong signals at chromosomal ends were observed with the second probe, indicating the presence of a telomeric motif (TTAGGGATGG)_n. This study represents the first FISH confirmation of the presence of a non-canonical telomeric motif not only for the infraorder Cimicomorpha but also for the suborder Heteroptera (Hemiptera) as a whole. The present finding is of key significance for unraveling the evolutionary shifts in the telomeric sequences in this suborder. Full article