Search Results (1,868)

The Verbesina gender represents the second most diverse group from the Asteraceae family in Mexico; Verbesina sphaerocephala is one of the most distributed species along the Mexican territory. This species has been poorly studied, reporting the presence of some bioactive compounds with antioxidant and antibacterial activity. In this study, phenolic and flavonoid contents and composition, antioxidant and antifungal activities of aqueous extracts of the stem of V. sphaerocephala and its fractions were determined. The results showed that the highest antifungal activity against Botrytis cinerea was shown by the aqueous extract (IC₅₀: 0.10 mg/mL) and the ethyl acetate fraction (IC₅₀: 14.8 mg/mL). In addition, the aqueous extract and the ethyl acetate fraction exhibited the highest phenolic (21.40 and 21.26 mg gallic acid equivalents per gram of dry extract, respectively) and flavonoid contents (11.53 and 3.71 mg rutin equivalents, respectively) and high antioxidant activity determined by the Total Antioxidant Capacity (20.62 and 40.21 mg ascorbic acid equivalents per gram of dry extract, respectively), Ferric Reducing Power (74.76 and 129.57 mg gallic acid equivalents per gram of dry extract, respectively), DPPH (IC₅₀: 12.38 and 7.36 mg/mL, respectively), and ABTS (IC₅₀: 5.60 and 7.76 mg/mL, respectively) methods. Twelve phenolic compounds were detected in the aqueous extract using UPLC-MS analysis, of which the major ones were protocatechuic, vanillic, and hydroxybenzoic acid, while in the ethyl acetate fraction, the presence of 18 phenolic compounds were identified, of which the majority were vanillin, rutin, and hydroxybenzoic acid. The results of this research demonstrate that the aqueous extract of V. sphaerocephala stems has phenolic compounds with antifungal and antioxidant activity. Full article

Aim: This study aims to estimate equivalent doses (EqDs) and life attributable risks (LARs) for pediatric patients who underwent chest–abdominal–pelvic (CAP) CT examinations in Madinah, Saudi Arabia. Methodology: This retrospective study collected data from 120 pediatric patients who underwent CAP CT examinations. The data were categorized by the age and gender of the pediatric patients. Then, the EqDs were computed using the NCICT (National Cancer Institute dosimetry system for computed tomography) program, and LARs were estimated from the equivalent dose (EqD) results using age- and gender-specific cancer risk models found in the Committee on the Biological Effects of Ionizing Radiation (BEIR) VII Phase 2 (2006). Results: The EqD range was 0.9 to 7.55 mSv for the prostate and colon (males and females), respectively. LARs for female breast and lung cancers were considered to have the highest values among the age groups. Nevertheless, LARs of the colon, liver, and leukemia cancers were higher for males than females. The LAR range of cancer incidence was 0.6 to 63.1 per 100,000 cases for prostate (aged 10–≤15 years) and breast (females aged 1≤–<5 years), respectively. The LAR range of cancer mortality was 0.1 to 41.9 per 100,000 cases for prostate (aged 10–≤15 years) and lung (females aged 1≤–<5 years). Conclusions: LARs of all cancer incidence and mortality from CAP CT examination were higher for pediatric females than males (with an average of 54%). This highlights the importance of considering pediatric patient gender and implementing optimization and protective measures in CAP CT examinations. LARs of breast and lung (for females) and colon (for males) cancers were found to have the highest values among the age groups. However, LARs of cancer incidence and mortality for colon, liver, and leukemia for males were higher than those for females. Full article

Background: Evidence regarding the performance-related effects of Actovegin is limited, despite legislated restrictions being in place for this supplement within sport settings. Objectives: Our study examined the effects of Actovegin on physiological responses and performance during maximal cardiopulmonary exercise in collegiate athletes. Methods: A randomized, double-blind, placebo-controlled experimental design was adopted. Moderately trained collegiate athletes from various sports were randomly allocated to placebo (n = 8) or Actovegin (n = 8) groups. All athletes consumed three capsules across each day for seven days of loading. Athletes underwent two separate cardiopulmonary exercise tests one week apart. Separate 2 × 2 mixed ANOVAs and effect sizes (ηp2) were used to assess for between- and within-group differences. Results: A significant time * group effect (p = 0.036, ηp2 = 0.278) was observed in systolic blood pressure. Significant main effects were only observed for time in several variables, with increases in peak oxygen uptake (VO₂) (p < 0.001, ηp2 = 0.893), peak minute ventilation (p = 0.004, ηp2 = 0.456), ventilatory equivalents for carbon dioxide (p = 0.002, ηp2 = 0.517), oxygen pulse (p = 0.006, ηp2 = 0.434), VO₂ at first ventilatory threshold (p = 0.002, ηp2 = 0.520), velocity at second ventilatory threshold (p < 0.001, ηp2 = 0.997), VO₂ at second ventilatory threshold (p < 0.001, ηp2 = 0.628), and peak velocity (p = 0.010, ηp2 = 0.386), and a decrease in respiratory exchange ratio (p < 0.001, ηp2 = 0.695). Conclusions: Our findings suggest that although physiological and performance alterations were evident with Actovegin supplementation during cardiopulmonary exercise, no further benefits beyond those obtained with a placebo were attained. Full article

(1) Introduction: Enhanced Recovery After Surgery (ERAS) protocols can create a cultural shift that will benefit patients by significantly reducing patient length of stay when compared to an equivalent group of surgical patients not following an ERAS protocol. (2) Methods: In this retrospective study of 2236 patients in a multi-center, community-based healthcare system, matching was performed based on a multitude of variables related to demographics, comorbidities, and surgical outcomes across seven ERAS protocols. These cohorts were then compared pre and post ERAS protocol implementation. (3) Results: ERAS protocols significantly reduced hospital length of stay from 3.0 days to 2.1 days (p <0.0001). Additional significant outcomes included reductions in opioid consumption from 40 morphine milligram equivalents (MMEs) to 20 MMEs (p <0.001) and decreased pain scores on postoperative day zero (POD 0), postoperative day one (POD 1), and postoperative day two (POD 2) when stratified into mild, moderate, and severe pain (p <0.001 on all three days). (4) Conclusions: ERAS protocols aggregately reduce hospital length of stay, pain scores, and opioid consumption. Full article

With China’s rapid economic development in recent years, enhancing the sense of well-being among citizens has become a critical objective. However, the interests of various stakeholder groups are often overlooked in decision-making surrounding land use. In this study, Yongsheng County, Yunnan Province serves as a case study for land use scenario simulations. The equivalent factor method is combined with Participatory Rural Appraisal (PRA) to investigate the relationship between ecosystem multifunctionality (EMF) and the equity index of multiple stakeholder groups in various land use scenarios. We also explore whether an optimal combination of land use types exists. The results indicate that (1) The current ecosystem service value in Yongsheng County is primarily driven by climate regulation and biodiversity conservation, with a relatively high functional value index but a comparatively low equity index; (2) Different stakeholder groups mainly prioritize food production and ecosystem services impacting food production, such as water resource provision and climate regulation; (3) A land use allocation pattern of 20% farmland, 4% water bodies, 21% mixed forest, 20% coniferous forest, and 35% grassland appears to provide the optimal EMF index while simultaneously achieving the optimal equity index across stakeholder groups. This research may offer valuable insights for optimizing land use planning while taking into account the well-being of diverse stakeholder groups. It also may have practical implications for the formulation of innovative land use management strategies. Full article

To investigate the effects of the combined addition of Lactiplantibacillus plantarum and sucrose on the fermentation weight loss (FWL), fermentation quality, and microbial community structure of ensiled rape straw under varying packing density conditions. After harvesting, the rapeseed straw was collected, cut into 1–2 cm pieces, and sprayed with sterile water to adjust the moisture content to 60%. The straw was then divided into two groups: one treated with additives (1 × 10⁵ CFU/g fresh material of Lactiplantibacillus plantarum and 10 kg/t fresh material of sucrose), and the other sprayed with an equivalent amount of sterile water as the control (CK). The treated materials were thoroughly mixed and packed into silos at densities of 450, 500, and 550 kg/m³. FWL was recorded on days 1, 3, 6, 15, 20, and 45 of fermentation. On day 45, the samples were analyzed for fermentation quality, microbial counts, and microbial diversity. FWL increased significantly (p < 0.05) in both the treated (LS) and control groups during fermentation. The LS group showed higher lactic acid (LA) levels (p < 0.05) and lower ammonia nitrogen levels (p < 0.05) compared to CK. The CK group had significantly higher (p < 0.05) counts of Coliforms and lower bacterial counts (p < 0.05) than LS. The dominant genera in the silage were Xanthomonas, Lactiplantibacillus plantarum, and Lentilactobacillus. In the LS group, the relative abundances of Lactiplantibacillus plantarum and Lentilactobacillus ranged from 16.93% to 20.43% and 15.63% to 27.46%, respectively, with their combined abundance being higher than in CK. At a packing density of 500 kg/m³, the relative abundances of Lactiplantibacillus plantarum and Lentilactobacillus in the LS group were significantly higher (p < 0.05) than in CK. Increasing packing density and applying additives to rape straw silage effectively reduced FWL, improved fermentation quality, boosted the relative abundance of beneficial lactic acid bacteria, and decreased the presence of undesirable bacteria such as Enterobacter and Bacillus. Full article

The completeness of landslide inventories and the selection of evaluation models significantly impact the accuracy of landslide susceptibility assessments. Conventional field geological survey methods and single remote-sensing technology struggle to reliably identify landslides under complex environmental conditions. Moreover, prevalent landslide susceptibility evaluation models are often plagued by issues such as subjectivity and overfitting. Therefore, we investigated the uncertainty in susceptibility modeling from the aspects of landslide inventory quality and model selection. The study focused on Luquan County in Yunnan Province, China. Leveraging multisource remote-sensing technologies, particularly emphasizing optical remote sensing and InSAR time-series deformation detection, the existing historical landslide inventory was refined and updated. This updated inventory was subsequently used to serve as samples. Nine evaluation indicators, encompassing factors such as distance to faults and tributaries, lithology, distance to roads, elevation, slope, terrain undulation, distance to the main streams, and average annual precipitation, were selected on the basis of the collation and organization of regional geological data. The information value and two coupled machine-learning models were formulated to evaluate landslide susceptibility. The evaluation results indicate that the two coupled models are more appropriate for susceptibility modeling than the single information value (IV) model, with the random forest model optimized by genetic algorithm in Group I2 exhibiting higher predictive accuracy (AUC = 0.796). Furthermore, comparative evaluation results reveal that, under equivalent model conditions, the incorporation of a remote-sensing landslide inventory significantly enhances the accuracy of landslide susceptibility assessment results. This study not only investigates the impact of landslide inventories and models on susceptibility outcomes but also validates the feasibility and scientific validity of employing multisource remote-sensing technologies in landslide susceptibility assessment. Full article

The role of autophagy goes far beyond the elimination of damaged cellular components and the quality control of proteins. It also cleanses cells from inclusions, including pathogenic viruses, and provides energy-forming components. The liver, which is an organ with increased metabolism, is made up of cells that are particularly vulnerable to damage. Therefore, detoxification of liver cells in the process of autophagy has become a very important issue clinically. The aim of this study was an immunohistochemical evaluation of proteins activated in rat liver cells at different stages of hyperbaric autophagy. The rats used for the study were randomly divided into six equivalent groups—three control groups and three experimental groups. Animals from the experimental groups were subjected to hyperbaric treatment in a hyperbaric chamber, with a pressure of 1.6 ATA for 120 min. They breathed atmospheric air. Rats were decapitated within 5 or 10 days after removal from the chamber. Immunohistochemical reactions with beclin 1, LC3B, RAB7, and HSC73 proteins were carried out on preparations made from liver slices. A three-step labeled streptavidin–biotin detection method of paraffin blocks (LSAB three-step) was used for immunohistochemical research. The results were evaluated using computer programs for morphometric analysis of microscopic images by calculating the mean surface areas occupied by a positive immunohistochemical reaction in individual groups for all antibodies tested. Increased closure of substrates in the autophagosome (beclin 1) induced late endosome transport and accelerated autophagosome maturation process (RAB7). Furthermore, a larger number of autophagosomes (LC3B) was observed in liver cells immediately after the cessation of hyperbaric activity; however, this decreased after 5 days. During this time, chaperone-mediated autophagy (HSC73) was observed on a larger scale. This means that increased macroautophagy induced by hyperbaric treatment weakens with time that has elapsed since the cessation of high pressure, whereas similarly induced chaperone-mediated autophagy intensifies over time. Full article

Eight porcine parvovirus (PPV) species, designated as PPV1 through PPV8, have been identified in swine. Despite their similarities, knowledge about their distribution and genetic differences remains limited, resulting in a gap in the genetic classification of these viruses. In this study, we conducted a comprehensive analysis using PPV1 to PPV7 genome sequences from Colombia and others available in the GenBank database to propose a classification scheme for all PPVs. Sera from 234 gilts aged 180 to 200 days were collected from 40 herds in Colombia. Individual detection of each PPV (PPV1 through PPV7) was performed using end-point PCR. Complete nucleotide (nt) sequencing was performed on the PPV1 viral protein (VP), and near-complete genome (NCG) sequencing was carried out for novel porcine parvoviruses (nPPVs) (PPV2 through PPV7). Phylogenetic analyses were conducted by comparing PPV1-VP sequences to 94 available sequences and nPPVs with 565 NCG, 846 nPPV-VP, and 667 nPPV–nonstructural protein (NS) sequences. Bayesian phylogenetic analysis was used to estimate substitution rates and the time to the most recent common ancestor for each PPV. The highest prevalence was detected for PPV3 (40.1%), followed by PPV5 (20.5%), PPV6 (17%), PPV1 (14.5%), PPV2 (9.8%), PPV4 (4.2%), and PPV7 (1.3%). Notably, all tested sera were negative for PPV8 genomes. An analysis of the PPV1-VP sequences revealed two main clades (PPV1-I and PPV1-II), with the sequences recovered in this study grouped in the PPV1-II clade. Comparative analysis showed significant genetic distances for PPV2 to PPV7 at the NCG (>6.5%), NS (>6.3%), and VP (>7.5%) regions, particularly when compared to equivalent regions of PPV genomes recovered worldwide. This study highlights the endemic circulation of nPPVs in Colombian pig herds, specifically among gilts. Additionally, it contributes to the phylogenetic classification and evolutionary studies of these viruses. The proposed method aims to categorize and divide subtypes based on current knowledge and the genomes available in databanks. Full article

Algebraic expressions and equalities can be constructed arbitrarily in a given algebraic framework according to the operational rules provided, and thus it is a prominent and necessary task in mathematics and applications to construct, classify, and characterize various simple general algebraic expressions and equalities. As an update to this prominent topic in matrix algebra, this article reviews and improves upon the well-known block matrix methodology and matrix rank methodology in the construction and characterization of matrix equalities. We present a collection of fundamental and useful formulas for calculating the ranks of a wide range of block matrices and then derive from these rank formulas various valuable consequences. In particular, we present several groups of equivalent conditions in the characterizations of the Hermitian matrix, the skew-Hermitian matrix, the normal matrix, etc. Full article

Globally, suicide mortality rates have decreased in this century; however, it has been reported that the decreasing trends of some subgroups were attenuated in the late 2010s. These tendencies are pronounced among adolescents. In this study, the temporal fluctuations of the age-standardized death rate of suicide (ASDR-suicide), crude mortality rate of suicide (CMR-suicide), and percentage of suicides per total deaths (PTD-suicide) among adolescents (10–24 years) in the global high-income and middle-income countries and in the Western Pacific Region (WPR) from 1990 to 2019, as well as the fixed effects of communication factors (penetration of cellular telephones and the Internet) on the ASDR-suicide/CMR-suicide/PTD-suicide of adolescents, were analyzed using the WHO Mortality Database. The male ASDR-suicide in global middle-income countries decreased, and those of males in global high-income countries and females in middle-income countries were unchanged, while the rate of other groups (males in the WPR and females in high-income countries and the WPR) increased. The ASDR-suicide of adolescent males in the WPR was almost equivalent to that of males in global high-income and middle-income countries in the 1990s but was higher than the rate in the 2010s. The ASDR-suicide of adolescent females in the WPR was consistently higher than that of females in global high-income and middle-income countries from 1990 to 2019, but the differences widened over time. The PTD-suicide of both males and females in middle-income countries was unchanged, whereas the PTD-suicide in global high-income countries and the WPR increased over time. The PTD-suicide in the WPR markedly increased by >40% in the late 2010s. The fixed effects of cellular telephone and Internet penetrations were negatively and positively related to adolescent suicide in the WPR, respectively. Therefore, these results suggest that, in this century, suicide prevention in adolescent health activities has been increasing in importance in the WPR compared with other regions. Full article

Background: The main cause of hospitalization in patients with heart failure is hypervolemia. Therefore, the primary treatment strategy involves diuretic therapy using intravenous loop diuretics to achieve decongestion and euvolemia. Some patients with acutely decompensated heart failure (ADHF) do not respond well to diuretic treatment, which may be due to diuretic resistance (DR). Such cases require high doses of diuretic medications and combination therapy with diuretics of different mechanisms of action. Although certain predisposing factors for diuretic resistance have been identified (such as hypotension, type 2 diabetes, impaired renal function, and hyponatremia), further research is needed to identify other pathophysiological markers of DR. Objective: This study aims to identify admission markers that can predict a high requirement for intravenous diuretics in hospitalized patients with decompensated heart failure. Methods: This study included 102 adult patients hospitalized for ADHF. At admission, patients underwent clinical assessment, laboratory parameter evaluation (including the N-terminal prohormone of brain natriuretic peptide [NT-proBNP] levels), and hemodynamic assessment using impedance cardiography (ICG). Hemodynamic profiles were based on the use of parameters such as heart rate (HR), blood pressure (BP), and thoracic fluid content (TFC) as markers of volume status. The analysis included 97 patients with documented doses of intravenous diuretic use. Patients were stratified into two groups based on median diuretic consumption (equivalent to 540 mg of intravenous furosemide): the high-loop diuretic utilization (LDU) group (n = 49) and the low-LDU group (n = 48). Results: Compared to low-LDU patients, high-LDU patients had greater thoracic fluid content at admission, both quantitatively (37.4 ± 8.1 vs. 34.1 ± 6.9 kOhm-1; p = 0.024) and qualitatively (TFC ≥ 35 kOhm-1: 59.2% vs. 33.3%; p = 0.011). Anemia was more common in the high-LDU group (67.4% vs. 43.8%; p = 0.019), as was elevated NT-proBNP (≥median of 3952 pg/mL: 60.4% vs. 37.5%; p = 0.024). High LDU was associated with a significantly longer hospitalization duration (12.9 ± 6.4 vs. 7.0 ± 2.6 days; p < 0.001). Logistic regression analysis identified anemia, elevated NT-proBNP, and high TFC as predictors of high LDU (HR: 2.65, 2.54, and 2.90, respectively). In a multifactorial model, only high TFC remained an independent predictor (HR: 2.60, 95% CI 1.04–6.49; p = 0.038). Conclusions: TFC was the sole independent admission marker of a high requirement for intravenous diuretics in patients hospitalized for decompensated heart failure. An objective assessment of volume status by impedance cardiography may support intensive personalized decongestion therapy. Full article

Background: The purpose of this study is to evaluate the effectiveness of Defocus Incorporated Multiple Segments (DIMSs) in slowing myopia progression in pediatric patients as a function of age. Methods: This was a non-randomized experimenter-masked retrospective controlled observational study of European individuals aged 6–16 years with progressive myopia but no ocular pathology. We retrospectively reviewed the charts of the participants allocated to receive DIMS spectacles (Hoya^® MiyoSmart^®) or single-vision spectacle lenses (control group). Cycloplegic spherical equivalent (SE) and axial length (AL) were measured at baseline and at 12-, 24-, and 36-month follow-ups. The results were stratified by age into four groups: patients wearing DIMS spectacles older or younger than 10 years of age (group A, 20 patients mean age 13.6 ± 2.2, and group C, 20 patients mean age 9.0 ± 1.2) and age-matched control groups (group B, 18 patients mean age 13.2 ± 2.5, and group D, 22 patients mean age 8.5 ± 0.9). Results: At 36 months, SE and AL increase were significantly reduced in groups A and C, respectively, compared to groups B and D (p < 0.05). Linear regression analysis showed a significant correlation (p < 0.05) between patient age and myopia progression for SE in groups A and C, but only in group A for AL. Groups B and D did not show any significant correlation (p > 0.05). Conclusions: DIMS spectacles seem to slow myopia progression in pediatric patients; however, their effectiveness shows the greatest results in children older than 10 years of age. Moreover, our findings suggest that AL may be the more reliable parameter for evaluating myopia progression. Full article

Purpose: To characterize the histologic and molecular subtype distribution of, and survival from, breast cancer (BC) among Canadian women overall, and by stage and age at diagnosis. Methods: Invasive BC cases from the Canadian Cancer Registry for women aged 15–99 years between 2012 and 2017 in Canada, excluding Quebec, were examined using pre-existing mortality linkages. Stage at diagnosis, molecular, and histologic subtypes, and 5-year net survival (NS) by age, subtype, and stage were determined. Results: 107,271 women with BC were included. Luminal A was the most common subtype, present in increasing proportions as women aged, up to a maximum of 55% of cases in 70–74. Ductal and luminal A were most likely to be diagnosed at stage I, while HER2+ had the highest proportion of diagnosis at stage III; triple negative (TN) and unknown had the highest proportion of stage IV. For all stages combined, luminal A had a five-year NS of 98%, while TN was 74%. NS for stage I BC was 99–100% for all subtypes, excepting TN, which was 96%. Survival decreased with advancing stage, most markedly for TN, for which stage III was 47% and stage IV 7%. Survival by equivalent stage and subtype was comparable across age groups but declined in older age categories. Conclusions: The varying natural histories of BC subtypes and histologies can inform prognoses, health system economics, and screening practices. The NS of 96% or greater for stage I, regardless of subtype, highlights the importance of early detection for all subtypes of BC, especially in aggressive subtypes. Full article

Objective: We aimed to investigate the impact of multimodal analgesia on postoperative complications and opioid prescription on a national level. Methods: This retrospective cross-sectional study included n = 1,307,923 hysterectomies (01/2006–12/2022, Premier Healthcare claims data). Multimodal analgesia was defined as opioid use with the addition of non-opioid analgesic modes, grouped into four categories: opioid-only and 1, 2, or 3 or more additional non-opioid analgesics. Multivariable regression models measured associations between multimodal categories and outcomes (composite/respiratory/cardiac/gastrointestinal/genitourinary, and CNS complications, oral morphine milligram equivalents [MME], and length of hospital stay [LOS]). Odds ratios (OR) and 95% confidence intervals (CI) are reported. Results: Overall, 84.3% (1,102,812/1,307,923) received multimodal analgesia, of which 58.9%, 28.0%, and 13.1% received 1, 2, or 3 or more additional non-opioid analgesics, respectively. The odds of any composite complication (any ≥1 complication) decreased with the addition of 1, 2, 3, or more analgesic modalities (versus opioid-only): OR 0.66 (CI 0.64; 0.68), OR 0.63 (CI 0.61; 0.66), OR 0.65 (CI 0.62; 0.67), respectively. Similar patterns existed for respiratory, cardiac, and genitourinary complications. Opioid prescription decreased incrementally with 1,2, 3, or more non-opioid analgesic modalities by 9.51 mg (CI 11.16; 7.86) and 15.29 mg (CI 17.21; 13.37) and 29.35 mg (CI 31.79; 26.91) cumulative MME. LOS was reduced by 0.52 days (CI 0.54; 0.51), 0.49 days (CI 0.51; 0.47), and 0.40 days (CI 0.43; 0.38), respectively. Costs were reduced by $765 (CI 817; 714) or $479 (CI 539; 419) with 1 or 2 multimodal modes. Conclusions: These findings suggest substantial benefits of multimodal analgesia, including significant decreases in serious complications (especially respiratory, cardiac, and genitourinary), opioid consumption, and hospitalizations. Multimodal analgesia may facilitate safe and efficient pain management with optimized opioid consumption. Full article