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22 pages, 5047 KiB  
Article
Unlocking the Secrets of Corn: Physiological Responses and Rapid Forecasting in Varied Drought Stress Environments
by Wenlong Song, Kaizheng Xiang, Yizhu Lu, Mengyi Li, Hongjie Liu, Long Chen, Xiuhua Chen and Haider Abbas
Remote Sens. 2024, 16(22), 4302; https://doi.org/10.3390/rs16224302 (registering DOI) - 18 Nov 2024
Abstract
Understanding the intricate relationship between drought stress and corn yield is crucial for ensuring food security and sustainable agriculture in the face of climate change. This study investigates the subtle effects of drought stress on corn physiological, morphological, and spectral characteristics at different [...] Read more.
Understanding the intricate relationship between drought stress and corn yield is crucial for ensuring food security and sustainable agriculture in the face of climate change. This study investigates the subtle effects of drought stress on corn physiological, morphological, and spectral characteristics at different growth stages, in order to construct a new drought index to characterize drought characteristics, so as to provide valuable insights for maize recovery mechanism and yield prediction. Specific conclusions are as follows. Firstly, the impact of drought stress on corn growth and development shows a gradient effect, with the most significant effects observed during the elongation stage and tasseling stage. Notably, Soil and Plant Analyzer Development (SPAD) and Leaf Area Index (LAI) are significantly affected during the silking stage, while plant height and stem width remain relatively unaffected. Secondly, spectral feature analysis reveals that, from the elongation stage to the silking stage, canopy reflectance exhibits peak–valley variations. Drought severity correlates positively with reflectance in the visible and shortwave infrared bands and negatively with reflectance in the near-infrared band. Canopy spectra during the silking stage are more affected by moderate and severe drought stress. Thirdly, LAI shows a significant positive correlation with yield, indicating its reliability in explaining yield variations. Finally, the yield-related drought index (YI) constructed based on Convolutional Neural Network (CNN), Random Forest (RF) and Multiple Linear Regression (MLR) methods has a good effect on revealing drought characteristics (R = 0.9332, p < 0.001). This study underscores the importance of understanding corn responses to drought stress at various growth stages for effective yield prediction and agricultural management strategies. Full article
18 pages, 3388 KiB  
Article
In Vitro Evaluation of Iraqi Kurdistan Tomato Accessions Under Drought Stress Conditions Using Polyethylene Glycol-6000
by Nawroz Abdul-razzak Tahir, Kamaran Salh Rasul, Djshwar Dhahir Lateef, Rebwar Rafat Aziz and Jalal Omer Ahmed
Life 2024, 14(11), 1502; https://doi.org/10.3390/life14111502 (registering DOI) - 18 Nov 2024
Abstract
Drought is one of the major abiotic stresses that affect plant growth and productivity, and plant stress responses are affected by both the intensity of stress and genotype. In Iraqi Kurdistan, tomato plants play a significant role in the country’s economy. Due to [...] Read more.
Drought is one of the major abiotic stresses that affect plant growth and productivity, and plant stress responses are affected by both the intensity of stress and genotype. In Iraqi Kurdistan, tomato plants play a significant role in the country’s economy. Due to climate change, which causes soil moisture to diminish, the crop’s growth and yield have been dropping in recent years. Accordingly, the effects of simulated drought stress on germination parameters were assessed in 64 tomato accessions gathered from the Iraqi Kurdistan region in order to identify sensitive and tolerant accessions. In this respect, the responses associated with drought stress were observed phenotypically and biochemically. Germination percentage (GP) and morphological characteristics such as root length (RL), shoot length (SL), and shoot fresh weight (SFW) were significantly reduced in both stress treatments with polyethylene glycol (PEG-6000) (7.5% PEG and 15% PEG). On the other hand, significant changes in biochemical profiles such as proline content (PC), soluble sugar content (SSC), total phenolic content (TPC), antioxidant activity (AC), guaiacol peroxidase (GPA), catalase (CAT), and lipid peroxidation (LP) in tomato accessions were detected; all biochemical traits were increased in most tomato accessions under the PEG-induced treatments compared to the control treatment (0.0% PEG). Three tomato accessions (AC61 (Raza Pashayi), AC9 (Wrdi Be Tow), and AC63 (Sandra)) were found to be the most tolerant accessions under all drought conditions, whereas the performances of the other tested accessions (AC13 (Braw), AC30 (Yadgar), and AC8 (Israili)) were inferior. The OMIC analysis identified the biomarker parameters for differentiating the highly, moderately, and low tolerant groups as PC, SSC, and TPC. This study shows that early PEG-6000 screening for drought stress may help in choosing a genotype that is suitable for growth in water-stressed environments. Hence, Raza Pashayi, Wrdi Be Tow, and Sandra accessions, which had great performances under drought conditions, can be candidates for selection in a breeding program to improve the growth of plants and production in the areas that face water limits. Full article
(This article belongs to the Special Issue Plant Biotic and Abiotic Stresses 2024)
27 pages, 1154 KiB  
Review
A Critical Review of Recent Advances in Maize Stress Molecular Biology
by Lingbo Meng, Jian Zhang and Nicholas Clarke
Int. J. Mol. Sci. 2024, 25(22), 12383; https://doi.org/10.3390/ijms252212383 (registering DOI) - 18 Nov 2024
Abstract
With the intensification of global climate change and environmental stress, research on abiotic and biotic stress resistance in maize is particularly important. High temperatures and drought, low temperatures, heavy metals, salinization, and diseases are widespread stress factors that can reduce maize yields and [...] Read more.
With the intensification of global climate change and environmental stress, research on abiotic and biotic stress resistance in maize is particularly important. High temperatures and drought, low temperatures, heavy metals, salinization, and diseases are widespread stress factors that can reduce maize yields and are a focus of maize-breeding research. Molecular biology provides new opportunities for the study of maize and other plants. This article reviews the physiological and biochemical responses of maize to high temperatures and drought, low temperatures, heavy metals, salinization, and diseases, as well as the molecular mechanisms associated with them. Special attention is given to key transcription factors in signal transduction pathways and their roles in regulating maize stress adaptability. In addition, the application of transcriptomics, genome-wide association studies (GWAS), and QTL technology provides new strategies for the identification of molecular markers and genes for maize-stress-resistance traits. Crop genetic improvements through gene editing technologies such as the CRISPR/Cas system provide a new avenue for the development of new stress-resistant varieties. These studies not only help to understand the molecular basis of maize stress responses but also provide important scientific evidence for improving crop tolerance through molecular biological methods. Full article
(This article belongs to the Special Issue Recent Advances in Maize Stress Biology)
10 pages, 4032 KiB  
Communication
Driving Factors and Future Trends of Wildfires in Alberta, Canada
by Maowei Bai, Qichao Yao, Zhou Wang, Di Wang, Hao Zhang, Keyan Fang and Futao Guo
Fire 2024, 7(11), 419; https://doi.org/10.3390/fire7110419 (registering DOI) - 18 Nov 2024
Abstract
Departures from historical wildfire regimes due to climate change have significant implications for the structure and composition of forests, as well as for fire management and operations in the Alberta region of Canada. This study analyzed the relationship between climate and wildfire and [...] Read more.
Departures from historical wildfire regimes due to climate change have significant implications for the structure and composition of forests, as well as for fire management and operations in the Alberta region of Canada. This study analyzed the relationship between climate and wildfire and used a random forest algorithm to predict future wildfire frequencies in Alberta, Canada. Key factors driving wildfires were identified as vapor pressure deficit (VPD), sea surface temperature (SST), maximum temperature (Tmax), and the self-calibrated Palmer drought severity index (scPDSI). Projections indicate an increase in wildfire frequencies from 918 per year during 1970–1999 to 1151 per year during 2040–2069 under a moderate greenhouse gas (GHG) emission scenario (RCP 4.5) and to 1258 per year under a high GHG emission scenario (RCP 8.5). By 2070–2099, wildfire frequencies are projected to increase to 1199 per year under RCP 4.5 and to 1555 per year under RCP 8.5. The peak number of wildfires is expected to shift from May to July. These findings suggest that projected GHG emissions will substantially increase wildfire danger in Alberta by 2099, posing increasing challenges for fire suppression efforts. Full article
(This article belongs to the Special Issue Effects of Climate Change on Fire Danger)
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18 pages, 7512 KiB  
Article
Seasonal Dynamics and Source Apportionment of Heavy Metals in Road Dust: A Case Study of Liuzhou, China
by Lu Zhang, Jianping Qian, Jinrui Liu, Kangkang Niu and Huanrong Zhang
Sustainability 2024, 16(22), 10051; https://doi.org/10.3390/su162210051 - 18 Nov 2024
Abstract
The spatial distribution and seasonal variation in heavy metal pollution in road dust in Liuzhou, China, were investigated. Road dust samples were collected during both drought and wet periods, and the concentrations of nine heavy metals—Cr, Ni, Cu, Pb, Zn, Cd, As, Sb, [...] Read more.
The spatial distribution and seasonal variation in heavy metal pollution in road dust in Liuzhou, China, were investigated. Road dust samples were collected during both drought and wet periods, and the concentrations of nine heavy metals—Cr, Ni, Cu, Pb, Zn, Cd, As, Sb, and Hg—were analyzed. The analysis showed that all nine heavy metals were higher than the background values of Chinese urban soils in both the drought and wet periods, and the mean heavy metal contents of road dust in the dry period were higher than those in the wet period, except for Cd. In the assessment of the pollution of heavy metals in road dust, the results of Igeo showed that Cd and Hg were significant; the results of NI showed that Cr and Ni were more significant. The spatial analysis shows that the industrial concentration areas in the north and west of Liuzhou City are concentration areas of heavy metal pollution. A Pearson correlation analysis showed high correlation between Cd in road dust heavy metals and Cd in the surrounding soil. Source identification via PCA revealed four main contributors: metallurgical and coal-fired industries, mechanical manufacturing, green belt maintenance, and waste treatment facilities. A quantitative analysis via APCS-MLR modeling confirmed that metallurgical and coal-fired industrial sources are the most widespread and important pollution sources in Liuzhou. There is a significant increase in the contribution of waste treatment sources to Hg contamination during wet periods. Full article
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16 pages, 5131 KiB  
Article
Agronomic Performance and Technological Attributes of Sugarcane Cultivars Under Split-Irrigation Management
by Henrique Fonseca Elias de Oliveira, Fernando Henrique Arriel, Frederico Antônio Loureiro Soares, Edson Cabral da Silva, Marcio Mesquita, Thiago Dias Silva, Jhon Lennon Bezerra da Silva, Cleiton Mateus Sousa, Marcos Vinícius da Silva, Ailton Alves de Carvalho and Thieres George Freire da Silva
AgriEngineering 2024, 6(4), 4337-4352; https://doi.org/10.3390/agriengineering6040245 (registering DOI) - 18 Nov 2024
Abstract
In addition to being an important instrument in the search for increasingly greater productivity, agricultural production with adequate use of irrigation systems significantly minimizes the impact on water resources. To meet high productivity and yield, as well as industrial quality, a series of [...] Read more.
In addition to being an important instrument in the search for increasingly greater productivity, agricultural production with adequate use of irrigation systems significantly minimizes the impact on water resources. To meet high productivity and yield, as well as industrial quality, a series of studies on sugarcane cultivation are necessary. Despite being able to adapt to drought, sugarcane is still a crop highly dependent on irrigation to guarantee the best quality standards. Our study aimed to analyze the agronomic performance and technological attributes of two sugarcane cultivars, evaluating the vegetative and productive pattern, as well as the industrial quality of the cultivars RB92579 and SP80–1816, which were cultivated under split-irrigation management in the Sugarcane Research Unit of IF Goiano—Campus Ceres, located in the state of Goiás in the Central-West region of Brazil. A self-propelled sprinkler irrigation system (IrrigaBrasil) was used, duly equipped with Twin 120 Komet sprinklers (Fremon, USA). The cultivars were propagated vegetatively and planted in 0.25 m deep furrows with 1.5 m between rows. The experiment was conducted in a completely randomized design (CRD), with a bifactorial split-plot scheme (5 × 2), with four replications, where the experimental plots were subjected to one of the following five split-irrigation management systems: 00 mm + 00 mm; 20 mm + 40 mm; 30 mm + 30 mm; 40 mm + 20 mm; or 60 mm + 00 mm. At 60 and 150 days after planting (DAP), the following respective irrigation management systems were applied: 00 mm + 00 mm and 20 mm + 40 mm. Biometric and technological attributes, such as plant height (PH) and stem diameter (SD), were evaluated in this case at 30-day intervals, starting at 180 DAP and ending at 420 DAP. Measurements of soluble solids content (°Brix), apparent sucrose content (POL), fiber content (Fiber), juice purity (PZA), broth POL (BP), reducing sugars (RS), and total recoverable sugars (TRS) were made by sampling stems at harvest at 420 DAP. RB92579 showed total recoverable sugar contents 11.89% and 8.86% higher than those recorded for SP80–1816 under split-irrigation with 40 mm + 20 mm and 60 mm + 00 mm, respectively. Shoot productivity of RB92579 reached 187.15 t ha−1 under split-irrigation with 60 mm + 00 mm, which was 42.16% higher than the shoot productivity observed for SP80–1816. Both cultivars showed higher qualitative and quantitative indices in treatments that applied higher volumes of water in the initial phase of the culture, coinciding with the dry season. Sugarcane cultivar RB92579 showed a better adaptation to the prevailing conditions in the study than the SP80–1816 cultivar. Full article
(This article belongs to the Section Agricultural Irrigation Systems)
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18 pages, 3797 KiB  
Article
Bacillus cereus: An Ally Against Drought in Popcorn Cultivation
by Uéliton Alves de Oliveira, Antônio Teixeira do Amaral Junior, Samuel Henrique Kamphorst, Valter Jário de Lima, Fábio Lopes Olivares, Shahid Khan, Monique de Souza Santos, Jardel da Silva Figueiredo, Samuel Pereira da Silva, Flávia Nicácio Viana, Talles de Oliveira Santos, Gabriella Rodrigues Gonçalves, Eliemar Campostrini, Alexandre Pio Viana and Freddy Mora-Poblete
Microorganisms 2024, 12(11), 2351; https://doi.org/10.3390/microorganisms12112351 - 18 Nov 2024
Abstract
Despite the development of adapted popcorn cultivars such as UENF WS01, strategies such as bacterial inoculation are being explored to enhance plant resilience to abiotic stress. This study investigates the impact of drought stress on popcorn cultivation. Specifically, the aim was to identify [...] Read more.
Despite the development of adapted popcorn cultivars such as UENF WS01, strategies such as bacterial inoculation are being explored to enhance plant resilience to abiotic stress. This study investigates the impact of drought stress on popcorn cultivation. Specifically, the aim was to identify the benefits of Bacillus cereus interaction with the drought-tolerant hybrid UENF WS01 for its morphophysiology and growth by comparing inoculated and non-inoculated plants under water-stressed (WS) and well-watered (WW) conditions. This evaluation was conducted using a randomized complete block design in a factorial arrangement. For WS with inoculation samples, there were significant increases in relative chlorophyll content, maximum fluorescence intensity, and agronomic water use efficiency. Chlorophyll content increased by an average of 50.39% for WS samples, compared to a modest increase of 2.40% for WW samples. Both leaf and stem biomass also significantly increased for WS relative to WW conditions. Overall, B. cereus inoculation mitigated the impact of water stress, significantly enhancing the expression of physiological and morphological traits, even when paired with a drought-tolerant hybrid. Full article
(This article belongs to the Section Plant Microbe Interactions)
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19 pages, 19897 KiB  
Article
A Novel Rainfall Classification for Mapping Rainwater Harvesting: A Case Study in Kalar, Iraq
by Kawa Z. Abdulrahman, Shvan F. Aziz and Moses Karakouzian
Water 2024, 16(22), 3311; https://doi.org/10.3390/w16223311 (registering DOI) - 18 Nov 2024
Abstract
Increasing water demand driven by population growth and climate change strains water resources, especially in arid regions. The effectiveness of rainwater harvesting (RWH) as a viable solution is contingent upon the meticulous selection of appropriate sites. Contemporary efforts have increasingly utilized Geographic Information [...] Read more.
Increasing water demand driven by population growth and climate change strains water resources, especially in arid regions. The effectiveness of rainwater harvesting (RWH) as a viable solution is contingent upon the meticulous selection of appropriate sites. Contemporary efforts have increasingly utilized Geographic Information Systems (GIS) and remote sensing technologies to optimize the identification of ideal locations for implementing RWH infrastructure. However, inconsistencies in rainfall classification methodologies can compromise the accuracy of the resulted suitability maps. Consequently, a standardized approach to grading rainfall depth for mapping RWH sites becomes imperative. This study presents an innovative rainfall classification method tailored for both micro and macro catchment areas, offering a reliable and adaptable approach to rainfall analysis. By refining classification criteria, this method aims to improve the consistency and precision of RWH mapping, addressing a gap in existing methodologies and providing a more standardized approach. Through the application of FAHP and Fuzzy overlay techniques in ArcGIS 10.4, the study compares traditional rainfall classification with the proposed new classification method to assess RWH suitability in Kalar. The comparison highlights that the new rainfall classification-based map yielded higher accuracy and realism compared to traditional methods. Full article
(This article belongs to the Special Issue Hydroclimate Extremes: Causes, Impacts, and Mitigation Plans)
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13 pages, 1411 KiB  
Article
The Applications of Different Glycine Betaine Doses on Young Pear Trees Under Drought Stress Conditions
by Cenk Küçükyumuk, Zeliha Küçükyumuk, Burhanettin İmrak and Songül Çömlekçioğlu
Horticulturae 2024, 10(11), 1217; https://doi.org/10.3390/horticulturae10111217 - 18 Nov 2024
Viewed by 42
Abstract
This experiment was conducted at the Fruit Research Institute, MAREM, Eğirdir-Isparta, Turkey, to detect the effects of different glycine betaine doses in drought stress conditions on young pear trees in 2019. The pear trees used in the study were one-year-old Deveci (Pyrus [...] Read more.
This experiment was conducted at the Fruit Research Institute, MAREM, Eğirdir-Isparta, Turkey, to detect the effects of different glycine betaine doses in drought stress conditions on young pear trees in 2019. The pear trees used in the study were one-year-old Deveci (Pyrus Comminus L.) variety grafted onto OHxF 333 rootstock. There were three different irrigation treatments in the experiment. Treatments were I100 treatment—available soil water reached field capacity for each irrigation, 100% (control); I50 treatment—irrigated with 50% of the water used in the I100 treatment (50% water deficit, moderate stress); I25 treatment—irrigated with 25% of the water used in the I100 treatment (75% water deficit, severe stress). Four different GB doses were used: GB0: 0, control; GB1: 1 mg L−1; GB5: 5 mg L−1; and GB10: 10 mg L−1. GB was not applied to pear trees in the I100 treatment. That is, there were nine different treatments in this study. GB applications provided a 19% increase in Pn of both the I25 and I50 treatments. According to the results of gsw, gsw increased between 18.0% and 27.8% for GB50 and GB25 treatments, respectively. In total, 10.9% and 14.8% increasing rates in shoot length were detected in GB10 applications in both the 50% and 75% water deficit treatments. The highest trunk diameter and fresh root weight results were determined in 10 mg L−1 GB dose applications under 50% water deficit conditions (I50GB10). Full article
(This article belongs to the Special Issue Responses to Abiotic Stresses in Horticultural Crops—2nd Edition)
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20 pages, 5190 KiB  
Article
Effects of Species-Rich Perennial Inter-Row Cover on Weed Flora and Soil Coverage in an Apple Orchard: A Case Study of Opportunities and Limitations in a Dry Continental Climate
by Barbara Ferschl, Magdolna Zita Szalai, Attila Gere, Tamás Kocsis and Zsolt Kotroczó
Agronomy 2024, 14(11), 2716; https://doi.org/10.3390/agronomy14112716 - 18 Nov 2024
Viewed by 75
Abstract
The planting of inter-row cover vegetation has long been practised in Western European fruit-growing regions, yet research in this area remains limited in many countries. With the rise of organic farming, the role and selection of inter-row cover species are becoming increasingly important. [...] Read more.
The planting of inter-row cover vegetation has long been practised in Western European fruit-growing regions, yet research in this area remains limited in many countries. With the rise of organic farming, the role and selection of inter-row cover species are becoming increasingly important. Proper species selection enhances weed control and promotes biodiversity, supporting flowering plants and beneficial insects in orchards. The six-year research aimed to develop a multi-species, perennial, multifunctional inter-row cover species mixture for a dry continental climate, which is suitable for soil protection and the displacement of heat-loving, drought-tolerant, invasive weed species. There was another expectation for this native seed mixture to be able to regenerate spontaneously and to be sustainable in the orchard for a long time with minimal maintenance. Based on our results, the two multi-species mixtures (15 and 18 species) successfully reduced the cover of weed species, and their composition changed in the direction of the natural flora characteristic of the area. According to general management practices, native species could not widely appear in the control rows created by mowing the local weed flora. Full article
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22 pages, 5572 KiB  
Article
Application of Machine Learning and Hydrological Models for Drought Evaluation in Ungauged Basins Using Satellite-Derived Precipitation Data
by Anjan Parajuli, Ranjan Parajuli, Mandip Banjara, Amrit Bhusal, Dewasis Dahal and Ajay Kalra
Climate 2024, 12(11), 190; https://doi.org/10.3390/cli12110190 - 17 Nov 2024
Viewed by 237
Abstract
Drought is a complex environmental hazard to ecosystems and society. Decision-making on drought management options requires evaluating and predicting the extremity of future drought events. In this regard, quantifiable indices such as the standardized precipitation index (SPI), the standardized precipitation evapotranspiration index (SPEI), [...] Read more.
Drought is a complex environmental hazard to ecosystems and society. Decision-making on drought management options requires evaluating and predicting the extremity of future drought events. In this regard, quantifiable indices such as the standardized precipitation index (SPI), the standardized precipitation evapotranspiration index (SPEI), and the standardized streamflow index (SSI) have been commonly used to characterize meteorological and hydrological drought. In general, the estimation and prediction of the indices require an extensive range of precipitation (SPI and SPEI) and discharge (SSI) datasets in space and time domains. However, there is a challenge for long-term and spatially extensive data availability, leading to the insufficiency of data in estimating drought indices. In this regard, this study uses satellite precipitation data to estimate and predict the drought indices. SPI values were calculated from the precipitation data obtained from the Centre for Hydrometeorology and Remote Sensing (CHRS) data portal for a study water basin. This study employs a hydrological model for calculating discharge and drought in the overall basin. It uses random forest (RF) and support vector regression (SVR) as machine learning models for SSI prediction for time scales of 1- and 3-month periods, which are widely used for establishing interactions between predictors and predictands that are both linear and non-linear. This study aims to evaluate drought severity variation in the overall basin using the hydrological model and compare this result with the machine learning model’s results. The results from the prediction model, hydrological model, and the station data show better correlation. The coefficients of determination obtained for 1-month SSI are 0.842 and 0.696, and those for the 3-month SSI are 0.919 and 0.862 in the RF and SVR models, respectively. These results also revealed more precise predictions of machine learning models in the longer duration as compared to the shorter one, with the better prediction result being from the SVR model. The hydrological model-evaluated SSI has 0.885 and 0.826 coefficients of determination for the 1- and 3-month time durations, respectively. The results and discussion in this research will aid planners and decision-makers in managing hydrological droughts in basins. Full article
(This article belongs to the Special Issue Coping with Flooding and Drought)
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18 pages, 3462 KiB  
Article
Evaluating Physiological and Yield Indices of Egyptian Barley Cultivars Under Drought Stress Conditions
by Wessam A. Abdelrady, Elsayed E. Elshawy, Hassan A. Abdelrahman, Syed Muhammad Hassan Askri, Zakir Ibrahim, Mohamed Mansour, Ibrahim S. El-Degwy, Taha Ghazy, Aziza A. Aboulila and Imran Haider Shamsi
Agronomy 2024, 14(11), 2711; https://doi.org/10.3390/agronomy14112711 - 17 Nov 2024
Viewed by 216
Abstract
Climate change significantly threatens crops, mainly through drought stress, affecting barley, which is essential for food and feed globally. Ten barley cultivars were evaluated under normal and drought stress conditions during the 2019/20 and 2020/21 seasons, focusing on traits such as days to [...] Read more.
Climate change significantly threatens crops, mainly through drought stress, affecting barley, which is essential for food and feed globally. Ten barley cultivars were evaluated under normal and drought stress conditions during the 2019/20 and 2020/21 seasons, focusing on traits such as days to heading and maturity, plant height, number of spikes m−2, spike length, 1000-kernel weight, and biological and grain yield. Drought stress significantly reduced most of these traits. The genotypes showed significant differences in their responses to irrigation treatments, with the interaction between seasons and cultivars also being significant for most traits. The grain yield and 1000-kernel weight were among the least affected traits under drought stress, respectively. Notably, Giza138 and Giza126 showed strong drought tolerance, suitable for drought-resilient breeding. In season one, Giza126, Giza134, and Giza138 yielded 13%, 9%, and 11%, respectively, while Giza135 and Giza129 showed higher reductions at 31% and 39%. In season two, Giza126, Giza134, and Giza138 had reductions of 14%, 10%, and 13%, respectively, while Giza135 and Giza129 again exhibited higher reductions at 31% and 38%. These cultivars also showed strong performance across various stress tolerance indices, including the MP, YSI, STI, GMP, and YI. Giza 134 demonstrated the lowest values for the SDI and TOL, indicating superior drought stress tolerance. On the other hand, Giza 129 and Giza 135 were the most sensitive to drought stress, experiencing significant reductions across critical traits, including 6.1% in days to heading, 18.37% in plant height, 28.21% in number of kernel spikes−1, 38.45% in grain yield, and 34.91% in biological yield. In contrast, Giza 138 and Giza 2000 showed better resilience, with lower reductions in the 1000-kernel weight (6.41%) and grain yield (10.61%), making them more suitable for drought-prone conditions. Giza 126 and Giza 132 also exhibited lower sensitivity, with minimal reductions in days to heading (2%) and maturity (2.4%), suggesting potential adaptability to water-limited environments. Giza 126 maintained the highest root lengths and had the highest stomatal conductance. Giza 138 consistently had the highest chlorophyll content, with SPAD values decreasing to 79% under drought. Despite leading in shoot length, Giza 135 decreased to 42.59% under drought stress. In conclusion, Giza 126 and Giza 138 showed adaptability to water-limited conditions with minimal impact on phenological traits. Giza 126 had the longest roots and highest stomatal conductance, while Giza 138 consistently maintained a high chlorophyll content. Together, they and Giza 134 are valuable for breeding programs to improve barley drought tolerance. Full article
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16 pages, 1319 KiB  
Article
Mechanism of Population Outflow and Ecological Pressure Reflux in China’s Pastoral Area: S-C Gacha’s Case
by Mingming Fan and Gongbu Zeren
Land 2024, 13(11), 1936; https://doi.org/10.3390/land13111936 - 17 Nov 2024
Viewed by 199
Abstract
In China’s rural areas, as populations flow into cities, dependence on land has significantly decreased, leading to widespread land abandonment; however, the relationship between population and land in pastoral areas differs. This study conducted a five-year tracking survey on Sunite Right Banner in [...] Read more.
In China’s rural areas, as populations flow into cities, dependence on land has significantly decreased, leading to widespread land abandonment; however, the relationship between population and land in pastoral areas differs. This study conducted a five-year tracking survey on Sunite Right Banner in Xilingol League (Inner Mongolia), where macro data and in-depth home interviews were used for the mechanisms’ analysis. The findings demonstrate that the pastoral regions’ ecological pressure has increased with population immigration. There are several factors influencing such interaction between people and rangeland: First is the cost mechanism. The increasing cost of livestock production leads herders to increase their reliance on grazing rental systems to increase the number of livestock. The grazing rental system generates income for those migrating to adjacent urban regions. Second is the institutional mechanism. The “three-separating property right systems”, a recent rural land institutional reform policy, has marketized rural individualized rangelands, leading herders to focus more on the exchange value of rangelands rather than the use value, including ecological functions. Consequently, both lessors and lessees engage in high-intensity utilization of their individualized grazing lands. Third is the natural mechanism. Climate fluctuations influence herders’ migration patterns, prompting them to leave and return to grazing lands. These ecological dynamics force herders to oscillate between survival and development, intensifying pressure on the grasslands during favorable years and preventing them from recovering while increasing the occurrence of poor years, such as droughts. Fourth is the consumption mechanism. Herders who have migrated to urban regions must continue relying on rangeland resources to sustain their livelihoods. However, lower economic outputs of livestock production hardly catch up with the higher consumption levels in urban regions, which leads migrated herders to increase their pressure on rural rangelands. The final factor is a combination of the previous four mechanisms. The interplay between these previously mentioned four mechanisms encouraged higher herder population outflow, though their reliance on rangeland resources has continued. Based on the results, we argue that these dynamic relations between rural population change and land use patterns are becoming common issues across pastoral regions in Inner Mongolia, demanding innovative solutions. Full article
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15 pages, 8589 KiB  
Article
Genome-Wide Identification and Expression Analysis of the Alfalfa (Medicago sativa L.) U-Box Gene Family in Response to Abiotic Stresses
by Shuaixian Li, Xiuhua Chen, Meiyan Guo, Xiaoyue Zhu, Wangqi Huang, Changhong Guo and Yongjun Shu
Int. J. Mol. Sci. 2024, 25(22), 12324; https://doi.org/10.3390/ijms252212324 - 17 Nov 2024
Viewed by 326
Abstract
E3 ubiquitin ligases known as plant U-box (PUB) proteins regulate a variety of aspects of plant growth, development, and stress response. However, the functions and characteristics of the PUB gene family in alfalfa remain unclear. This work involved a genome-wide examination of the [...] Read more.
E3 ubiquitin ligases known as plant U-box (PUB) proteins regulate a variety of aspects of plant growth, development, and stress response. However, the functions and characteristics of the PUB gene family in alfalfa remain unclear. This work involved a genome-wide examination of the alfalfa U-box E3 ubiquitin ligase gene. In total, 210 members were identified and divided into five categories according to their homology with the members of the U-box gene family in Arabidopsis thaliana. The phylogenetic analysis, conserved motifs, chromosomal localization, promoters, and regulatory networks of this gene were investigated. Chromosomal localization and covariance analyses indicated that the MsPUB genes expanded MsPUB gene family members through gene duplication events during evolution. MsPUB genes may be involved in the light response, phytohormone response, growth, and development of several biological activities, according to cis-acting element analysis of promoters. In addition, transcriptome analysis and expression analysis by qRT-PCR indicated that most MsPUB genes were significantly upregulated under cold stress, drought stress, and salt stress treatments. Among them, MsPUBS106 and MsPUBS185 were significantly and positively correlated with cold resistance in alfalfa. MsPUBS110, MsPUBS067, MsPUBS111 and MsPUB155 were comprehensively involved in drought stress, low temperature, and salt stress resistance. All things considered, these discoveries offer fresh perspectives on the composition, development, and roles of the PUB gene family in alfalfa. They also provide theoretical guidance for further investigations into the mechanisms regulating the development, evolution, and stress tolerance of MsPUB. Full article
(This article belongs to the Special Issue Advance in Plant Abiotic Stress: 2nd Edition)
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Article
Integrating Morpho-Physiological, Biochemical, and Molecular Genotyping for Selection of Drought-Tolerant Pigeon Pea (Cajanus cajan L.) Genotypes at Seedling Stage
by Benjamin O. Ouma, Kenneth Mburu, Geoffrey K. Kirui, Edward K. Muge and Evans N. Nyaboga
Plants 2024, 13(22), 3228; https://doi.org/10.3390/plants13223228 (registering DOI) - 16 Nov 2024
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Abstract
Pigeon pea (Cajanus cajan (L.) Millsp.), a potential legume as an economic source of protein, is commonly cultivated in tropical and subtropical regions of the world. It possesses medicinal properties and acts as a cash crop, benefiting low-income farmers economically. The identification [...] Read more.
Pigeon pea (Cajanus cajan (L.) Millsp.), a potential legume as an economic source of protein, is commonly cultivated in tropical and subtropical regions of the world. It possesses medicinal properties and acts as a cash crop, benefiting low-income farmers economically. The identification of pigeon peas exhibiting drought tolerance has become crucial in addressing water scarcity issues in the agriculture sector. In addition, exploring the genetic diversity among genotypes is important for conservation, management of genetic resources, and breeding programs. The aim of this study was to evaluate the morpho-physiological and biochemical responses of selected pigeon pea genotypes under pot-induced water stress conditions through different field capacities as well as the genetic diversity using start codon targeted (SCoT) markers. A significant variation was observed for the physiological traits studied. The accumulation of fresh weight (FW) and dry weight (DW) was significantly reduced in moderate and severe drought stress conditions. The lowest % DW decrease was found in LM (35.39%), KAT (39.43%), and SM (46.98%) than other genotypes at severe drought stress. Analyses of physiological responses including the photosynthetic efficiency (Phi2), the chlorophyll content (SPAD), and the relative water content (RWC) revealed positive and negative correlations with various parameters, reflecting the impact of drought stress on the chlorophyll content. The results revealed that biochemical traits including the total phenolic content, soluble sugars, proline, total protein, total amino acids, and free amino acids were variably and significantly increased under water stress. Antioxidant enzyme activity levels, specifically ascorbate peroxidase (APX) and catalase, varied among the genotypes and in response to severe water stress, offering further insights into adaptive responses. The eight genotypes analysed by use of 20 SCoT markers revealed 206 alleles and an average of 10.3 alleles per locus. Genetic similarity ranged from 0.336 to 0.676, clustering the pigeon pea genotypes into two major groups by the unweighted pair group method of arithmetic averages (UPGMA) cluster analysis. Principal coordinate analysis (PCoA) explained 43.11% of genetic variation and based on analysis of molecular variance, a high genetic variation (80%) within populations was observed, emphasizing the potential for genetic improvement. Among the eight genotypes studied, LM and KAT were drought tolerant and genetically diverse and therefore could be used as parents for developing drought tolerance in pigeon pea. Full article
(This article belongs to the Section Plant Response to Abiotic Stress and Climate Change)
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