Search Results (267)

The impact of porcine circovirus (PCV) on the worldwide pig industry is profound, leading to notable economic losses. Early and prompt identification of PCV is essential in managing and controlling this disease effectively. A range of detection techniques for PCV have been developed and primarily divided into two categories focusing on nucleic acid or serum antibody identification. The methodologies encompass conventional polymerase chain reaction (PCR), real-time fluorescence quantitative PCR (qPCR), fluorescence in situ hybridization (FISH), loop-mediated isothermal amplification (LAMP), immunofluorescence assay (IFA), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA). Despite their efficacy, these techniques are often impeded by the necessity for substantial investment in equipment, specialized knowledge, and intricate procedural steps, which complicate their application in real-time field detections. To surmount these challenges, a sensitive, rapid, and specific PCV detection method using Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)-Cas12a/13a coupled with isothermal amplification, such as enzymatic recombinase amplification (ERA), recombinase polymerase amplification (RPA), and loop-mediated isothermal amplification (LAMP), has been developed. This novel method has undergone meticulous optimization for detecting PCV types 2, 3, and 4, boasting a remarkable sensitivity to identify a single copy per microliter. The specificity of this technique is exemplary, with no observable interaction with other porcine viruses such as PEDV, PRRSV, PRV, and CSFV. Its reliability has been validated with clinical samples, where it produced a perfect alignment with qPCR findings, showcasing a 100% coincidence rate. The elegance of merging CRISPR-Cas technology with isothermal amplification assays lies in its on-site testing without the need for expensive tools or trained personnel, rendering it exceptionally suitable for on-site applications, especially in resource-constrained swine farming environments. This review assesses and compares the process and characteristics inherent in the utilization of ERA/LAMP/RPA-CRISPR-Cas12a/Cas13a methodologies for the detection of PCV, providing critical insights into their practicality and effectiveness. Full article

The primary objective of this paper is to identify the critical components of the acoustic field for a piston-type pressure reducing valve (PRV) with a high pressure reduction ratio, as well as to predict unfavorable noise both experimentally and numerically. The numerical calculations were conducted using a hybrid approach that combines computational fluid dynamics (CFD) and computational aeroacoustics (CAA). Flow-induced pressure fluctuation from unsteady turbulent flow extracted by the throttling cone, the valve body and the baffle in the low-pressure chamber were considered as individual dipole acoustic sources during calculation of the internal acoustic field. The results indicated that the selected three dipole acoustic sources always played a vital role in the response of the acoustic field, and none of them could be ignored. In comparison, the throttling cone had the most salient contribution to acoustic field distribution, the valve body took second place, and the baffle had the least salient contribution. The radiated noise of interest was predicted using the indirect boundary element method (IBEM), incorporating all three components as dipole acoustic sources simultaneously; the numerical noise values showed strong validation against the experimental data. Furthermore, the distribution of sound pressure levels, as well as directional and planar field points, is also presented. This paper provides new insights into the role of each component in flow-induced noise, and offers technical support for noise reduction design and optimization of pressure reducing valves. Full article

In this work, an automatic distribution of the pressure reduction valves (PRVs) is proposed. First, a well-calibrated hydraulic model is required. The model of Manresa, a city of Catalunya in the Mediterranean area, was calibrated using pressure sensors, and the background leakage was estimated using weighted emitter coefficients. Simulating the model in real boundary conditions highlights the areas of maximum background leakage. The manual introduction of a PRV shows its effectiveness regarding leakage reduction. An algorithm for finding the high-pressure areas and their boundary pipes is presented. The introduction of the PRV, taking into account the flow constraints, produces a new scenario. Finally, the leakage reduction thanks to the pressure control by means of new actuators is evaluated. The leakage is reduced by around 6%. Full article

Background: Pseudorabies virus (PRV) is a highly infectious pathogen that affects a wide range of mammals and imposes a significant economic burden on the global pig industry. The viral envelope of PRV contains several glycoproteins, including glycoprotein E (gE) and glycoprotein B (gB), which play critical roles in immune recognition, vaccine development, and diagnostic procedures. Mutations in these glycoproteins may enhance virulence, highlighting the need for updated vaccines. Method: This review examines the functions of PRV gE and gB in vaccine development and diagnostics, focusing on their roles in viral replication, immune system interaction, and pathogenicity. Additionally, we explore recent findings on the importance of gE deletion in attenuated vaccines and the potential of gB to induce immunity. Results: Glycoprotein E (gE) is crucial for the virus’s axonal transport and nerve invasion, facilitating transmission to the central nervous system. Deletion of gE is a successful strategy in vaccine development, enhancing the immune response. Glycoprotein B (gB) plays a central role in viral replication and membrane fusion, aiding viral spread. Mutations in these glycoproteins may increase PRV virulence, complicating vaccine efficacy. Conclusion: With PRV glycoproteins being essential to both vaccine development and diagnostic approaches, future research should focus on enhancing these components to address emerging PRV variants. Updated vaccines and diagnostic tools are critical for combating new, more virulent strains of PRV. Full article

Pressure control by means of pressure-reducing valves (PRVs) is a possible strategy to reduce water losses in water distribution networks (WDNs). However, PRV replacement with energy-harvesting devices—such as pumps as turbines (PATs)—can lead to a more sustainable management of water systems. This study analyzes the case study of a WDN located in Northern Italy, of which the layout is supposed to be upgraded by installing a PAT for both pressure reduction and energy recovery. To identify the optimal PAT to install (i.e., the one that maximizes energy recovery), a fleet of forty-five turbomachines is hypothetically employed. The study reveals that the hydraulic regulation of the optimal PAT allows recovering over 50% of the hydraulic energy available in the WDN. Full article

The Penacova-Régua-Verin (PRV) and the Manteigas-Vilariça-Bragança (MVB) are two of the longest faults of the Iberian Peninsula. These faults striking NNE–SSW, over lengths of >200 km, were developed during late-Variscan Orogeny and reactivated in response to the Alpine Cycle tectonics. Their tectonic evolution during Alpine compression (Cenozoic) and their implication in the active tectonic activity of Iberia are under discussion. Their recent tectonic activity is recorded in the vertical offset of geomorphological surfaces, in the associated pull-apart basins, and in M > 7 paleoseismic events. Based on the vertical surface offset of Pliocene surfaces (140–300 m for the MVB fault and 150–200 m for the PRV), together with the horizontal offset (1300–1600 m for MVBF fault and 600–1400 m for PRVF), we can conclude that they were reactivated as left-lateral strike-slip faults with a reverse component during the Pliocene (3.6 Ma)–present. These results indicate that these faults are not related to the strain transmission during the collision with Eurasia (Eocene–Oligocene). However, they are related to the intraplate strain of the southern collision with the African plate during the Upper Neogene. The estimated slip-rate is 0.2–0.5 mm/a for both faults. These slip-rates evidence important implications for the seismic hazard of this intraplate region. Full article

Zika virus (ZIKV), a mosquito-borne flavivirus, is a significant global health concern due to its association with neurodevelopmental disorders such as congenital Zika syndrome (CZS). This study aimed to compare the replication kinetics, viral persistence, cytopathogenic effects, and immune gene expression in human microglia cells (CHME-3) infected with an Asian lineage ZIKV (PRVABC59, referred to as ZIKV-PRV) and an African lineage ZIKV (IBH30656, referred to as ZIKV-IBH). We found that ZIKV-PRV replicated more efficiently and persisted longer while inducing lower levels of cell death and inflammatory gene activation compared with ZIKV-IBH. These findings suggest that the enhanced replication and persistence of ZIKV-PRV, along with its ability to evade innate immune responses, may underlie its increased neuropathogenic potential, especially in the context of CZS. In contrast, ZIKV-IBH, with its stronger immune gene activation and higher cytopathogenicity, may lead to more acute infections with faster viral clearance, thereby reducing the likelihood of chronic central nervous system (CNS) infection. This study provides crucial insights into the molecular and cellular mechanisms driving the differential pathogenicity of ZIKV lineages and highlights the need for further research to pinpoint the viral factors responsible for these distinct clinical outcomes. Full article

Porcine sapelovirus (PSV) is a new pathogen that negatively impacts the pig industry in China. Affected pigs experience severe diarrhea and even death. Vaccination is used to control disease outbreaks, and sensitive diagnostic methods that can distinguish infected animals from vaccinated animals (DIVA) are essential for monitoring the effectiveness of disease control programs. Tests based on the detection of the nonstructural protein (NSP) 3AB are reliable indicators of viral replication in infected and vaccinated animals. In this study, the recombinant PSV 3AB protein was expressed by a prokaryotic expression system, and an indirect ELISA method was established. Serum samples from healthy animals, immunized animals, and infected animals were evaluated. The ELISA method identified 3AB with high sensitivity (99.78%) and specificity (100.0%), and no cross-reaction was observed with serum antibodies against porcine reproductive and respiratory syndrome virus (PRRSV), infection with classical swine fever virus (CSFV), pseudorabies virus (PRV), bovine viral diarrhea virus (BVDV), porcine epidemic diarrhea virus (PEDV), or foot-and-mouth disease virus (FMDV). The ELISA method described here can effectively distinguish infected and vaccinated animals and is an important inexpensive tool for monitoring serum and controlling PSV. Full article

Most existing approaches to ensuring water quality in water distribution systems (WDSs) are deterministic, i.e., they do not consider uncertainties, although they may have significant impacts on the water quality. It is well recognized that water demand represents a predominant uncertainty in a WDS. In addition, water age is often used as an important parameter to describe the water quality in a WDS and can be influenced by water demand and control elements such as pressure-reducing valves (PRVs). Therefore, the aim of this study is to carry out a probabilistic analysis of the impact of demand uncertainty on the water age in the distribution network. Based on the solution of deterministic optimization to minimize the water age, Monte Carlo simulation will be carried out by sampling the uncertain demand to evaluate the stochastic distribution of water age, as well as other operating variables like pressure and flow. As a result, the probability of violating the constraints of such variables can be determined, with the reliability of the operating strategy (e.g., the settings of the PRVs) given by deterministic optimization provided. In cases of low reliability, it is necessary to modify the operating strategy in order to decrease the probability of constraint violation. For this purpose, a chance-constrained optimization problem is formulated, and its benefits for ensuring the user-defined reliability are studied. A benchmark network is used to verify the proposed approach. Full article

Purpose: Short-track speed skating results in high-energy crashes with an elevated risk of head injury. The goal of this study was to evaluate the resulting kinematics of an anti-rotation helmet technology for speed skating. Methods: Two traditional rigid foam speed-skating helmets (BT and ST) were compared with one anti-rotation speed skating helmet (MIPS). Each helmet was impacted with a pneumatic device across three locations. The resulting linear or rotational accelerations (PLA or PRA) and rotational velocities (PRV) were measured with accelerometers placed on a Hybrid III head form. Additionally, the head impact criterion (HIC) was calculated from accelerations and the brain injury criterion (BrIC) was obtained from rotational velocities. Results: MIPS showed significantly higher values of accelerations (PLA = 111.24 ± 9.21 g and PRA = 8759.11 ± 2601.81 rad/s²) compared with the other helmets at all three impact locations (p < 0.01, ES = 3.00 to 4.11). However, velocities were lowest, but not significantly different, for the MIPS helmet (25.77 ± 1.43 rad/s). Furthermore, all resulting kinematics except peak linear accelerations were significantly different across impact locations. Conclusion: Helmet designs specific to the collision characteristics of speed skating may still be lacking, but would decrease the risk of sport-related concussions. Full article

Oncolytic viruses combined with immunotherapy offer significant potential in tumor therapy. In this study, we engineered a further attenuated pseudorabies virus (PRV) vaccine strain that incorporates a PD-L1 inhibitor and demonstrated its promise as an oncolytic virus in tumor therapy. We first showed that the naturally attenuated PRV vaccine strain Bartha can efficiently infect tumor cells from multiple species, including humans, mice, and dogs in vitro. We then evaluated the safety and anti-tumor efficacy of this vaccine strain and its different single-gene deletion mutants using the B16-F10 melanoma mouse model. The TK deletion strain emerged as the optimal vector, and we inserted a PD-L1 inhibitor (iPD-L1) into it using CRISPR/Cas9 technology. Compared with the control, the recombinant PRV (rPRV-iPD-L1) exhibited more dramatic anti-tumor effects in the B16-F10 melanoma mouse model. Our study suggests that PRV can be developed not only as an oncolytic virus but also a powerful vector for expressing foreign genes to modulate the tumor microenvironment. Full article

Urban water distribution systems (WDSs) exhibit significant energy potential that is currently dissipated in the form of excess pressure, either at brake-pressure tanks (BPTs) or pressure reduction valves (PRVs). Recent research focuses on the implementation of energy harvesting methods within WDSs in order to improve the energy efficiency of such systems. This paper provides a systematic review of the technologies developed for energy exploitation in WDSs, covering both their technical and economic aspects, while considering their reliability in providing water pressure regulation. Drawn from the existing literature and state of the art, a systematic analysis was carried out that specifies and categorizes the most essential parameters that impact the implementation of energy recovery turbines into WDSs. Different turbine types, design parameters, and performance properties, such as generation efficiency and pressure regulation precision, were considered. Finally, practical challenges and consequences emerging from the joint optimization of water and power systems are addressed. Full article

Pseudorabies virus (PRV) is one of the herpes viruses that can infect a wide range of animals including pigs, cattle, sheep, mice, and wild animals. PRV is a neurotropic alphaherpesvirus capable of infecting a variety of mammals. There is a rising interest in the targeted application of probiotic bacteria to prevent viral diseases, including PRV. In this study, the surface expression of enhanced green fluorescent protein (EGFP) on recombinant Lactiplantibacillus plantarum NC8 (rNC8) through the LP3065 LPxTG motif of Lactobacillus plantarum WCFS1 was generated. The surface expression was observed through confocal microscopy. Dendritic cell targeting peptides (DCpep) were also fused with LPxTG that help to bind with mouse DCs. The PRV-gD was cloned in LP3065 LPxTG, resulting in the generation of rNC8-LP3065-gD. Inactivated rNC8-LP3065-gD was administered intravenously in mice on days 1 and 7 at a dose of 200 µL (10⁹ CFU/mouse) for monitoring immunogenicity. Subsequently, a challenge dose of PRV TJ (10⁴ TCID₅₀) was administered intramuscularly at 14 days post-immunization. The survival rate of the immunized mice reached 80% (4/5) with no significant signs of illness. A significant rise in anti-gD antibodies was detected in the immunized mice by ELISA. Quantitative PCR (qPCR) results showed decreased viral loading in different body tissues. Flow cytometry of lymphocytes derived from mice spleen indicated an increase in CD3⁺CD4⁺ T cells, but CD3⁺CD8⁺ T cells were not detected. Moreover, it offers a model to delineate immune correlates with rNC8-induced immunity against swine viral diseases. Full article

The purpose of this study is to more quantitatively identify changes in body function through various bio-signal parameters. (1) Background: Forest therapy is effective in stabilizing cognitive, emotional, cardiovascular, and autonomic nervous systems. In particular, it is necessary to more quantitatively confirm changes in body functions through various bio signals. (2) Methods: As a forest therapy program (FTP) for the elderly, it consisted of strength training in the forest, respiratory aerobic exercises, and cognitive function training, and a total of 19 sessions were performed for 12 weeks. The electroencephalography (EEG) and Photoplethysmography (PPG) before and after the program were measured and compared between program participants (FTP group) and non-participants (control group). (3) Results: the FTP group showed increase in the alpha band power in EEG and a decrease in the PRV index, Tad, and Tae after the program compared to the control group; (4) Conclusions: Significant differences occurred in the physiological functioning of the elderly participants after the program. This is a result that can confirm the effectiveness of forest therapy more quantitatively. Forest therapy has a positive effect on mental stress reduction and cardiovascular function. Full article

Detection methods have been developed to prevent transmission of zoonotic or xenozoonotic porcine viruses after transplantation of pig organs or cells to the recipient (xenotransplantation). Eleven xenotransplantation-relevant viruses, including porcine cytomegalovirus, porcine roseolovirus (PCMV/PRV), porcine lymphotropic herpesviruses -1, -2, -3 (PLHV-1, 2, 3), porcine parvovirus (PPV), porcine circovirus 2, 3, 4 (PCV2, 3, 4), hepatitis E virus genotype 3 (HEV3), porcine endogenous retrovirus-C (PERV-C), and recombinant PERV-A/C have been selected. In the past, several pig breeds, minipigs, and genetically modified pigs generated for xenotransplantation had been analyzed using these methods. Here, spleen, liver, and blood samples from 10 German slaughterhouse pigs were screened using both PCR-based and immunological assays. Five viruses: PCMV/PRV, PLHV-1, PLHV-3, and PERV-C, were found in all animals, and PCV3 in one animal. Some animals were latently infected with PCMV/PRV, as only virus-specific antibodies were detected. Others were also PCR positive in the spleen and/or liver, indicative of an ongoing infection. These results provide important information on the viruses that infect German slaughterhouse pigs, and together with the results of previous studies, they reveal that the methods and test strategies efficiently work under field conditions. Full article