Search Results (9,676)

Introduction. Pediatric traumatology is a complex field that requires a comprehensive understanding of physeal development, remodeling potential, and the ossification process in order to ensure appropriate patient treatment. The objective of this study was to assess the willingness of practicing physicians to participate in a telemedicine collaboration aimed at enhancing the exchange between the outpatient and inpatient sectors and promoting the digitalization of the pediatric sector. This is in response to the growing significance of digitalization in the medical field. Methods. A survey consisting of 15 items was sent to 800 practicing trauma surgeons, pediatric surgeons, and pediatricians within a 100 km radius of Hamburg, Germany. The survey included questions about the respondents’ professional experience and telemedicine experience, as well as inquiries about possible telemedicine collaborations. Results. The response rate was 19.3%. Less than half of the participants already used telemedicine in daily practice. In general, 75% of respondents expressed an interest in collaborating with the inpatient sector. The most common reasons for hospital referral were the need for surgery, inadequate treatment of children in practice and co-assessment. The majority were in favor of flexible communication, either via video telephony, imaging applications like or messaging applications. Conclusions. The study revealed a high level of interest in telemedicine collaboration. Information exchange should be tailored to individual needs, with practitioners requiring a versatile and personalized approach that includes imaging. Strict enforcement of data protection regulations is essential. Further research is needed to evaluate the effectiveness of telemedicine collaboration in the treatment of pediatric trauma in both hospital and outpatient settings. Full article

Advanced manufacturing techniques such as Additive Manufacturing (AM) have grown rapidly in major industries such as aerospace, automotive, and biomedical device manufacturing. Biomedical industry has benefitted immensely from AM because of its flexibility in design and its rapid production cycle. Powder bed processes are the major production technique for metal-based AM implants. This paper serves as a comprehensive review on the research efforts being made using AM to develop new patient centered medical devices. This review focuses on AM of the most common metals for biomedical applications, Magnesium alloys, Cobalt-Chromium alloys, pure Titanium, Titanium alloys. Several different aspects are discussed including biocompatibility and osseointegration, application of specific metals in different types of implants, their advantages and disadvantages, mechanical properties in comparison to bone, and their production technologies. Regulatory and quality assurance hurdles that are facing new innovations made using AM are discussed. Full article

Volume reconstruction techniques are gaining increasing interest in medical domains due to their potential to learn complex 3D structural information from sparse 2D images. Recently, neural radiance fields (NeRF), which implicitly model continuous radiance fields based on multi-layer perceptrons to enable volume reconstruction of objects at arbitrary resolution, have gained traction in natural image volume reconstruction. However, the direct application of NeRF to medical volume reconstruction presents unique challenges due to differences in imaging principles, internal structure requirements, and boundary delineation. In this paper, we evaluate different NeRF techniques developed for natural images, including sampling strategies, feature encoding, and the use of complimentary features, by applying them to medical images. We evaluate three state-of-the-art NeRF techniques on four datasets of medical images of different complexity. Our goal is to identify the strengths, limitations, and future directions for integrating NeRF into the medical domain. Full article

Approximately 30% of patients with epilepsy are drug-refractory. There is an urgent need to elucidate the exact pathophysiology of different types of epilepsies and the mechanisms of action of both antiseizure medication and metabolic therapies to treat patients more effectively and safely. For example, it has been demonstrated that exogenous ketone supplement (EKS)-generated therapeutic ketosis, as a metabolic therapy, may decrease epileptic activity in both animal models and humans, but its exact mechanism of action is unknown. However, it was demonstrated that therapeutic ketosis, among others, can increase adenosine level, which may enhance activity of A1 adenosine receptors (A₁Rs) in the brain. It has also been demonstrated previously that adenosine has anti-epileptic effect through A₁Rs in different models of epilepsies. Thus, it is possible that (i) therapeutic ketosis generated by the administration of EKSs may exert its anti-epileptic effect through, among other mechanisms, increased adenosine level and A₁R activity and that (ii) the enhanced activity of A₁Rs may be a necessary anti-epileptic mechanism evoked by EKS administration-generated ketosis. Moreover, EKSs can evoke and maintain ketosis without severe side effects. These results also suggest that the therapeutic application of EKS-generated ketosis may be a promising opportunity to treat different types of epilepsies. In this literature review, we specifically focus on the putative role of A₁Rs in the anti-epileptic effect of EKS-induced ketosis. Full article

Optical biosensors have emerged as a powerful tool in analytical biochemistry, offering high sensitivity and specificity in the detection of various biomolecules. This article explores the advancements in the integration of optical biosensors with microfluidic technologies, creating lab-on-a-chip (LOC) platforms that enable rapid, efficient, and miniaturized analysis at the point of need. These LOC platforms leverage optical phenomena such as chemiluminescence and electrochemiluminescence to achieve real-time detection and quantification of analytes, making them ideal for applications in medical diagnostics, environmental monitoring, and food safety. Various optical detectors used for detecting chemiluminescence are reviewed, including single-point detectors such as photomultiplier tubes (PMT) and avalanche photodiodes (APD), and pixelated detectors such as charge-coupled devices (CCD) and complementary metal–oxide–semiconductor (CMOS) sensors. A significant advancement discussed in this review is the integration of optical biosensors with pixelated image sensors, particularly CMOS image sensors. These sensors provide numerous advantages over traditional single-point detectors, including high-resolution imaging, spatially resolved measurements, and the ability to simultaneously detect multiple analytes. Their compact size, low power consumption, and cost-effectiveness further enhance their suitability for portable and point-of-care diagnostic devices. In the future, the integration of machine learning algorithms with these technologies promises to enhance data analysis and interpretation, driving the development of more sophisticated, efficient, and accessible diagnostic tools for diverse applications. Full article

Polymer 3D printing has is used in a wide range of applications in the medical field. Polyamide 12 (PA12) is a versatile synthetic polymer that has been used to reconstruct bony defects. Coating its surface with calcium phosphate compounds, such as hydroxyapatite (HA), could enhance its bonding with bone. The aim of this study was to coat 3D-printed polyamide 12 specimens with hydroxyapatite by a simple innovative surface treatment using light-cured resin cement. Polyamide 12 powder was printed by selective laser sintering to produce 80 disc-shaped specimens (15 mm diameter × 1.5 mm thickness). The specimens were divided randomly into two main groups: (1) control group (untreated), where the surface of the specimens was left without any modifications; (2) treated group, where the surface of the specimens was coated with hydroxyapatite by a new method using a light-cured dental cement. The coated specimens were characterised by both Fourier transform infrared spectroscopy (FTIR) and Transmission Electron Microscopy (TEM), (n = 10/test). The control and treated groups were further randomly subdivided into two subgroups according to the immersion in phosphate-buffered saline (PBS). The first subgroup was not immersed in PBS and was left as 3D-printed, while the second subgroup was immersed in PBS for 15 days (n = 10/subgroup). The surfaces of the control and treated specimens were examined using an environmental scanning electron microscope (SEM) and energy dispersive X-ray analysis (EDXA) before and after immersion in PBS. Following the standard American Society for Testing and Materials (ASTM D3359), a cross-cut adhesion test was performed. The results of the FTIR spectroscopy of the coated specimens were confirmed the HA bands. The TEM micrograph revealed agglomerated particles in the coat. The SEM micrographs of the control 3D-printed polyamide 12 specimens illustrated the sintered 3D-printed particles with minimal porosity. Their EDXA revealed the presence of carbon, nitrogen, and oxygen as atomic%: 52.1, 23.8, 24.1 respectively. After immersion in PBS, there were no major changes in the control specimens as detected by SEM and EDXA. The microstructure of the coated specimens showed deposited clusters of calcium and phosphorus on the surface, in addition to carbon, nitrogen, and oxygen, with atomic%: 9.5, 5.9, 7.2, 30.9, and 46.5, respectively. This coat was stable after immersion, as observed by SEM and EDXA. The coat adhesion test demonstrated a stable coat with just a few loose coating flakes (area removed <5%) on the surface of the HA-coated specimens. It could be concluded that the 3D-printed polyamide 12 could be coated with hydroxyapatite using light-cured resin cement. Full article

Anthranilic acids, salicylaldehydes and arylboronic acids reacted in EtOH/H₂O (1/3) at 150 °C under microwave irradiation for 1 h to give, in excellent yields and purity, twenty-three bridgehead bicyclo[4.4.0]boron heterocycles via one-pot, three-component green synthesis. The scope and the limitations of the reactions are discussed in terms of the substitution of ten different anthranilic acids, three salicylaldehydes and three arylboronic acids. The replacement of salicylaldehyde with o-hydroxyacetophenone demanded a lipophilic solvent for the reaction to occur. Eight novel derivatives were isolated following crystallization in a toluene-containing mixture that included molecular sieves. The above one-pot, three-component reactions were completed under microwave irradiation at 180 °C within 1.5 h, thus avoiding the conventional prolonged heating reaction times and the use of a Dean–Stark apparatus. All derivatives were studied for their affinity to calf thymus DNA using proper techniques like viscosity and UV–vis spectroscopy, where DNA-binding constants were found in the range 2.83 × 10⁴–8.41 × 10⁶ M⁻¹. Ethidium bromide replacement studies using fluorescence spectroscopy indicated Stern–Volmer constants between 1.49 × 10⁴ and 5.36 × 10⁴ M⁻¹, whereas the corresponding quenching constants were calculated to be between 6.46 × 10¹¹ and 2.33 × 10¹² M⁻¹ s⁻¹. All the above initial experiments show that these compounds may have possible medical applications for DNA-related diseases. Full article

Far-Infrared Radiation (FIR) is emerging as a novel non-invasive tool for mitigating inflammation and oxidative stress, offering potential benefits for certain medical conditions such as cardiovascular disease and chronic inflammatory disorders. We previously demonstrated that the application of patch-based FIR therapy on human umbilical vein endothelial cells (HUVECs) reduced the expression of inflammatory biomarkers and the levels of reactive oxygen species (ROS). Several in vitro studies have shown the inhibitory effects of FIR therapy on cell growth in different cancer cells (including murine melanoma cells), mainly using the wound healing assay, without direct cell motility or tracking analysis. The main objective of the present study was to conduct an in-depth analysis of single-cell motility and tracking during the wound healing assay, using an innovative high-throughput technique in the human melanoma cell line M14/C2. This technique evaluates various motility descriptors, such as average velocity, average curvature, average turning angle, and diffusion coefficient. Our results demonstrated that patch-based FIR therapy did not impact cell proliferation and viability or the activation of mitogen-activated protein kinases (MAPKs) in the human melanoma cell line M14/C2. Moreover, no significant differences in cell motility and tracking were observed between control cells and patch-treated cells. Altogether, these findings confirm the beneficial effects of the in vitro application of patch-based FIR therapy in human melanoma cell lines, although such effects need to be confirmed in future in vivo studies. Full article

DNA-based biosensors have emerged as cutting-edge tools with significant potential to revolutionize medical diagnostics and environmental monitoring. These biosensors leverage the specificity and sensitivity of DNA interactions to detect a wide range of biomolecular targets, making them ideal for early disease detection, genetic analysis, and real-time environmental assessment. Despite their promising applications, several challenges impede their widespread adoption. Key issues include the stability of DNA molecules, which are prone to degradation under environmental conditions, and the need for enhanced specificity and sensitivity to accurately detect target molecules in complex samples. Technological hurdles in miniaturizing and integrating these sensors into portable, user-friendly devices, along with ethical concerns regarding data privacy and the misuse of genetic information, also pose significant barriers. This systematic review examines the current state of DNA-based biosensor technology, highlights the main challenges, and discusses potential strategies to overcome these obstacles. By addressing these multifaceted issues through ongoing research and innovation, DNA-based biosensors can be developed into robust tools for various applications, contributing to improved public health outcomes and environmental sustainability. Full article

Bioimpedance imaging aims to generate a 3D map of the resistivity and permittivity of biological tissue from multiple impedance channels measured with electrodes applied to the skin. When the electrodes are distributed around the body (for example, by delineating a cross section of the chest or a limb), bioimpedance imaging is called electrical impedance tomography (EIT) and results in functional 2D images. Conventional EIT systems rely on individually cabling each electrode to master electronics in a star configuration. This approach works well for rack-mounted equipment; however, the bulkiness of the cabling is unsuitable for a wearable system. Previously presented cooperative sensors solve this cabling problem using active (dry) electrodes connected via a two-wire parallel bus. The bus can be implemented with two unshielded wires or even two conductive textile layers, thus replacing the cumbersome wiring of the conventional star arrangement. Prior research demonstrated cooperative sensors for measuring bioimpedances, successfully realizing a measurement reference signal, sensor synchronization, and data transfer though still relying on individual batteries to power the sensors. Subsequent research using cooperative sensors for biopotential measurements proposed a method to remove batteries from the sensors and have the central unit supply power over the two-wire bus. Building from our previous research, this paper presents the application of this method to the measurement of bioimpedances. Two different approaches are discussed, one using discrete, commercially available components, and the other with an application-specific integrated circuit (ASIC). The initial experimental results reveal that both approaches are feasible, but the ASIC approach offers advantages for medical safety, as well as lower power consumption and a smaller size. Full article

This study introduces a novel thermoacoustic (TA) focusing system enhanced by Airy beam-based acoustic metasurfaces, significantly improving acoustic focusing and efficiency. The system integrates a TA emitter, fabricated from carbon nanotube (CNT) films, with a binary acoustic metasurface capable of generating quasi-Airy beams. Through finite element simulations, the system’s heat conduction, acoustic focusing, and self-healing properties were thoroughly analyzed. The results demonstrate that the system achieves superior sub-wavelength focusing, tunable focal length via frequency control, and robust self-healing, even in the presence of obstacles. These findings address current limitations in TA emitters and suggest broader applications in medical ultrasound and advanced technology. Full article

Phage therapy, the use of bacteriophages (phages) to treat bacterial infections, is regaining momentum as a promising weapon against the rising threat of multidrug-resistant (MDR) bacteria. This comprehensive review explores the historical context, the modern resurgence of phage therapy, and phage-facilitated advancements in medical and technological fields. It details the mechanisms of action and applications of phages in treating MDR bacterial infections, particularly those associated with biofilms and intracellular pathogens. The review further highlights innovative uses of phages in vaccine development, cancer therapy, and as gene delivery vectors. Despite its targeted and efficient approach, phage therapy faces challenges related to phage stability, immune response, and regulatory approval. By examining these areas in detail, this review underscores the immense potential and remaining hurdles in integrating phage-based therapies into modern medical practices. Full article

The student–patient relationship represents the cornerstone of medical education, shaping future doctors’ knowledge, skills and attitudes. While most patients allow student involvement in their care, some may express discomfort. Thus, obtaining explicit consent is essential to respect patients’ right of autonomy. This study mainly aims to assess the practical application of informed consent by medical students and teachers regarding students’ presence and participation in patients’ care. An observational cross-sectional study was performed, and an online questionnaire was given to students and teachers at a single medical school, via institutional email. The study included 289 participants, namely 232 students and 57 teachers. While 81% of teachers reported always asking the patient’s consent for students’ presence, only 28% of students claimed this to be the case. Despite challenges like overcrowding and limited time, involving students in healthcare benefits both students and patients. Moreover, medical ethics education is crucial to foster compassionate care and promote ethical reasoning. The disparities found between teachers’ practices and students’ perspectives highlight the need to intervene and provide them with an adequate education on ethical values in clinical practice. Strategic interventions at institutional levels are required for a simultaneous high quality of patient care and clinical training. Full article

Breast cancer is one of the most lethal and widespread diseases affecting women worldwide. As a result, it is necessary to diagnose breast cancer accurately and efficiently utilizing the most cost-effective and widely used methods. In this research, we demonstrated that synthetically created high-quality ultrasound data outperformed conventional augmentation strategies for efficiently diagnosing breast cancer using deep learning. We trained a deep-learning model using the EfficientNet-B7 architecture and a large dataset of 3186 ultrasound images acquired from multiple publicly available sources, as well as 10,000 synthetically generated images using generative adversarial networks (StyleGAN3). The model was trained using five-fold cross-validation techniques and validated using four metrics: accuracy, recall, precision, and the F1 score measure. The results showed that integrating synthetically produced data into the training set increased the classification accuracy from 88.72% to 92.01% based on the F1 score, demonstrating the power of generative models to expand and improve the quality of training datasets in medical-imaging applications. This demonstrated that training the model using a larger set of data comprising synthetic images significantly improved its performance by more than 3% over the genuine dataset with common augmentation. Various data augmentation procedures were also investigated to improve the training set’s diversity and representativeness. This research emphasizes the relevance of using modern artificial intelligence and machine-learning technologies in medical imaging by providing an effective strategy for categorizing ultrasound images, which may lead to increased diagnostic accuracy and optimal treatment options. The proposed techniques are highly promising and have strong potential for future clinical application in the diagnosis of breast cancer. Full article

Nanoparticle adhesion to polymer and similar substrates may be prone to low nano-Newton forces, disrupting the surface bonds and patterning, potentially reducing the functionality of complex surface patterns. Testing this, a functionalised surface reported for biological and medical applications, consisting of a thin plasma-derived oxazoline-based film with 68 nm diameter covalently bound colloidal gold nanoparticles attached within an aqueous solution, underwent nanomechanical analysis. Atomic Force Microscopy nanomechanical analysis was used to quantify the limits of various adaptations to these nanoparticle-featured substrates. Regular and laterally applied forces in the nano-Newton range were shown to de-adhere surface-bound gold nanoparticles. Applying a nanometre-thick overcoating anchored the nanoparticles to the surface and protected the underlying base substrate in a one-step process to improve the overall stability of the functionalised substrate against lower-range forces. The thickness of the oxazoline-based overcoating displayed protection from forces at different rates. Testing overcoating thickness ranging from 5 to 20 nm in 5 nm increments revealed a significant improvement in stability using a 20 nm-thick overcoating. This approach underscores the importance of optimising overcoating thickness to enhance nanoparticle-based surface modifications’ durability and functional integrity. Full article