Search Results (20)

The remediation and exploitation of sandy saline soils, an underutilized resource, can be enhanced by a greater comprehension of the impact of plants and microorganisms on nutrient cycling. However, there is scant research information on the capacity of different trees and shrubs to improve carbon and nitrogen cycling in saline soils at different depth layers. This study investigated the effect of the trees Zelkova serrata (ZS) and Ligustrum lucidum (LL) and shrub Hibiscus syriacus (HS) on the carbon and nitrogen fractions, soil enzyme activities and microbial communities in sandy saline soils. Planting ZS, LL or HS improved soil quality, increased soil carbon and nitrogen content, changed rhizosphere soil metabolites and enhanced soil enzyme activities and microbial abundance and diversity. Compared to values in the bare soil, the highest reduction in soil salinity was noticed under Zelkova serrata (49%) followed by Ligustrum lucidum (48%). The highest increase in total soil organic carbon (SOC) was noted under Ligustrum lucidum and Hibiscus syriacus (62% each), followed by Zelkova serrata (43%), as compared to levels in the bare soil. In the 0–10 cm soil layer, the total N in bare soil was 298 ± 1.48 mg/kg, but after planting LL, ZS or HS, the soil total N increased by 101%, 56% and 40%, respectively. Compared with that of the bare soil, cbbL sequencing showed that the relative abundance of Bradyrhizobium increased and that of Bacillus decreased due to planting. Similarly, the nifH sequencing results indicated that the relative abundance of Bradyrhizobium and Motiliproteu increased and that of Desulfuromonas and Geoalkalibacter decreased. These findings suggested that soil microorganisms could play a pivotal role in the carbon and nitrogen cycle of saline soils by influencing the content of soil carbon and nitrogen. Full article

The Hyphomicrobiales (Rhizobiales) order contains soil bacteria with an irregular distribution of the Calvin–Benson–Bassham cycle (CBB). Key enzymes in the CBB cycle are ribulose 1,5-bisphosphate carboxylase/oxygenase (RuBisCO), whose large and small subunits are encoded in cbbL and cbbS, and phosphoribulokinase (PRK), encoded by cbbP. These genes are often found in cbb operons, regulated by the LysR-type regulator CbbR. In Bradyrhizobium, pertaining to this order and bearing photosynthetic and non-photosynthetic species, the number of cbbL and cbbS copies varies, for example: zero in B. manausense, one in B. diazoefficiens, two in B. japonicum, and three in Bradyrhizobium sp. BTAi. Few studies addressed the role of CBB in Bradyrhizobium spp. symbiosis with leguminous plants. To investigate the horizontal transfer of the cbb operon among Hyphomicrobiales, we compared phylogenetic trees for concatenated cbbL-cbbP-cbbR and housekeeping genes (atpD-gyrB-recA-rpoB-rpoD). The distribution was consistent, indicating no horizontal transfer of the cbb operon in Hyphomicrobiales. We constructed a ΔcbbLS mutant in B. diazoefficiens, which lost most of the coding sequence of cbbL and has a frameshift creating a stop codon at the N-terminus of cbbS. This mutant nodulated normally but had reduced competitiveness for nodulation and long-term adhesion to soybean (Glycine max (L.) Merr.) roots, indicating a CBB requirement for colonizing soybean rhizosphere. Full article

In the green alga Chlamydomonas reinhardtii, hydrogen production is catalyzed via the [FeFe]-hydrogenases HydA1 and HydA2. The electrons required for the catalysis are transferred from ferredoxin (FDX) towards the hydrogenases. In the light, ferredoxin receives its electrons from photosystem I (PSI) so that H₂ production becomes a fully light-driven process. HydA1 and HydA2 are highly O₂ sensitive; consequently, the formation of H₂ occurs mainly under anoxic conditions. Yet, photo-H₂ production is tightly coupled to the efficiency of photosynthetic electron transport and linked to the photosynthetic control via the Cyt b₆f complex, the control of electron transfer at the level of photosystem II (PSII) and the structural remodeling of photosystem I (PSI). These processes also determine the efficiency of linear (LEF) and cyclic electron flow (CEF). The latter is competitive with H₂ photoproduction. Additionally, the CBB cycle competes with H₂ photoproduction. Consequently, an in-depth understanding of light-driven H₂ production via photosynthetic electron transfer and its competition with CO₂ fixation is essential for improving photo-H₂ production. At the same time, the smart design of photo-H₂ production schemes and photo-H₂ bioreactors are challenges for efficient up-scaling of light-driven photo-H₂ production. Full article

One of the most important agricultural activities worldwide, coffee cultivation, is severely affected by the Coffee Berry Borer (CBB), Hypothenemus hampei, considered the primary coffee pest. The CBB is a tiny beetle that diminishes the quantity and quality of coffee beans by penetrating them to feed on the endosperm and deposit its eggs, continuing its life cycle. One strategy to combat CBBs is using biological control agents, such as certain species of ants. Here, a mathematical model (consisting of a system of nonlinear ordinary differential equations) is formulated to describe the prey–predator interaction between CBBs and an unspecified species of ants. From this mathematical perspective, the model allows us to determine conditions for the existence and stability of extinction, persistence or co-existence equilibria. Transitions among those equilibrium states are investigated through the maximum per capita consumption rate of the predator as a bifurcation parameter, allowing us to determine the existence of transcritical and saddle-node bifurcations. Phase portraits of the system are presented for different values of bifurcation parameter, to illustrate stability outcomes and the occurrence of bifurcations. It is concluded that an increase in bifurcation parameters significantly reduces the CBB population, suggesting that ant predation is an effective control strategy, at least theoretically. Full article

Carbon-sequestering microorganisms play an important role in the carbon cycle of wetland ecosystems. However, the response mechanism of carbon-sequestering microbial communities to wetland type changes and their relationship with soil carbon remain unclear. To explore these differences and identify the main influencing factors, this study selected marsh wetlands, river wetlands and lakeside wetlands around Qinghai Lake as research subjects. High-throughput sequencing was employed to analyze the functional gene cbbM of carbon-sequestering microorganisms. The results revealed that the alpha diversity of cbbM carbon-sequestering microorganisms mirrored the trend in total carbon content, with the highest diversity observed in marsh wetlands and the lowest in lakeside wetlands. The dominant bacterial phylum was Proteobacteria, with prevalent genera including Thiothrix, Acidithiobacillus, and Thiodictyon. Acidithiobacillus served as a biomarker in lakeside wetlands, while two other genera were indicative of marsh wetlands. The hierarchical partitioning analysis indicated that the diversity of cbbM carbon-fixing microorganisms was primarily influenced by the total nitrogen content, while the community structure was significantly affected by the soil total carbon content. Moreover, an increased soil temperature and humidity were found to favor the carbon fixation processes of Thiomicrospira, Thiomonas, Polaromonas, and Acidithiobacillus. In summary, changes in wetland types seriously affected the characteristics of cbbM carbon sequestration in microbial communities, and a warm and humid climate may be conducive to wetland carbon sequestration. Full article

Growing environmental awareness has prompted a resurgence in traditional building techniques that rely on natural or recycled materials since many believe that structures made from these resources are ecologically friendly. Using Life Cycle Assessment (LCA) for construction materials offers valuable insights into the impacts produced during their production and construction processes. This study aims to assess the environmental impacts of two different constructions—an Earthbag Building (EB) and a conventional Sahrawi Cement Blocks Building (CBB). It also determines whether it is more environmentally beneficial to construct traditionally, utilizing local materials and aligning with the principles of the circular economy, which is one of the Sustainable Development Goals (SDGs) in Europe. This study specifically examines a cradle-to-gate LCA, using the software Simapro v. 9.4.0.1. Results show that in 21 out of the 22 impact categories analyzed, the CBB performs worse, in some cases presenting an impact of 70% higher than the EB. The highest impact is obtained for fine particulate matter formation and Global Warming related to Human Health categories, whilst impact categories related to water consumption and eutrophication obtained an impact of less than 0.001 for both constructions. Full article

Bioactive compounds (BACs) and chemical building blocks (CBBs) play a pivotal role in driving economic growth. These compounds, known for their diverse applications in pharmaceuticals, agriculture, and manufacturing, have become integral to meeting the increasing demand for sustainable and innovative products. In this research, we used and characterized dynamic maceration to extract BACs and CBBs from broccoli leaves (BLs). A central composite design (CCD) was selected to evaluate the effect of temperature (from 4 °C to 70 °C), ethanol concentration (from 30% to 70% (v/v)), and exposition time (15 to 60 min) on total phenolic content (TPC) (mg of gallic acid equivalents (GAEs) per 100 g of dry biomass (db)). A confirmation experiment (CE) was performed to reproduce the optimal conditions (50 °C, 36.92 min, and 30% (v/v)) for BAC extraction. Results indicated a GAE concentration of 112.95 ± 0.92 mg/100 g db, while the statistical model predicted a value of 111.87 mg of GAEs/100 g db (error of 0.95%) with a rate constant (k) value of 0.0154 mg/g·min (R² of 0.9894). BACs and CBBs were identified with gas chromatography–electron impact mass spectrometry detecting l-isoleucine, l-leucine, malonic acid, and succinic acid, among others. Finally, a life cycle inventory (LCI) was developed to determine global warming (GW) and water consumption (WC), among others, for 10 g of BL extract. Findings reported herein prove the sustainability of eco-friendly extraction of BACs and CBBs for the effective use of agricultural by-products. Full article

Jamaica produces coffee marketed as Blue Mountain and high mountain (grown outside the Blue Mountains). Since the discovery of the coffee berry borer (CBB; Hypothenemus hampei) in Jamaica in 1978, chemical control has traditionally been the primary approach used to protect the crop from the pest. However, in the last 20 years, there has been an effort to shift towards more sustainable management strategies. The study was conducted to determine CBB activity (trap catch) and field infestation on coffee farms in the high mountains and Blue Mountains of Jamaica, over a crop cycle. A total of 27,929 and 12,921 CBBs were captured at high mountain and Blue Mountain farms, respectively. Peak CBB activity occurred in April in the high mountain region (365 CBBs/trap/month) and February in the Blue Mountain region (129 CBBs/trap/month). The highest levels of infestation were in November (33%) and October (34%) in the high mountain region and Blue Mountain region, respectively. There was no significant difference in the patterns of CBB activity and infestation between the study locations, and neither were related to the temperature or relative humidity. However, there was a significant relationship with rainfall. These data suggest that the population dynamics of the CBB may involve complex interactions among weather conditions, berry development, and agronomic practices. Full article

Studying the CO₂-assimilation potential under the effect of glucose addition is of great significance to completely comprehend the dynamic carbon cycle in wetland ecosystems. Rhizospheric sediments (RS) and bulk sediments (BS) were selected, with the addition of glucose (G) or not, and two experimental pots (RSG and BSG) and two control pots (RS and BS) were formed. Then, within 45 h of glucose addition, the sediments were sampled at intervals of 4 h for dynamic monitoring. The bacterial communities encoded by CO₂-assimilating function genes (cbbL) and the corresponding activities of key enzyme (ribulose-1,5-bisphosphate carboxylase oxygenase, RubisCO), and the light fraction (LF) and heavy fraction (HF) of organic carbon (C) and nitrogen (N) of the samples were determined. The results demonstrated that the dynamic processes of glucose deposition and degradation occurred in sediments from RSG and BSG, with the greatest depositions of 2.35 and 2.48 mg·g⁻¹ in the 4th and 12th hour, respectively. The contents of LFOC, LFON, HFOC, and HFON decreased by 171.70%, 125.45%, 8.40%, and 68.17% in the RSG pot, and decreased by 221.55%, 102.61%, 0.07%, and 74.74% in the BSG pot, respectively, which suggested the dominant activities of C and N mineralization. The FT-MIR of LF showed different changes of typical chemical bonds between RSG and BSG during the process, which further indicated irregular and inconsistent mineralization activities. The RubisCO activities in the rhizospheric sediments (52.14 nmol (g·min)⁻¹ on average) were substantially greater than in the bulk sediments, which indicated the high potential of carbon assimilation in rhizospheric sediments. Moreover, it showed a lower trend in BSG, BS, and RS, but an increasing trend in RSG after the glucose addition, albeit the effects were recovered in the 45th hour. The cbbL-containing bacteria were more abundant in the rhizospheric sediments than in the bulk sediments, and this effect was higher than that of the glucose addition. Proteobacteria were the dominating phylum with mean values of 93.49%, and Burkholderiales was found to be the dominant order (37.74% on average). Moreover, the changes in bacterial composition between the rhizospheric sediments and bulk sediments were more pronounced than they were during the process. Therefore, the effects of glucose degradation on RubisCO activity and cbbL-containing bacteria were transient, but the effects on organic matter fractions were straightforward, which probably further change the bacterial abundance and composition. Full article

The roles of bacterial communities in the health of soil microenvironments can be more adequately defined through longer-term soil management options. Carbon dioxide (CO₂) fixation by autotrophic bacteria is a principal factor in soil carbon cycles. However, the information is limited to how conservation tillage practices alter soil physiochemical properties, autotrophic bacterial communities, and microbial catabolic diversity. In this study, we determined the changes in autotrophic bacterial communities and carbon substrate utilization in response to different soil management practices. A replicated field study was established in 2001, with the following soil treatments arranged in a randomized complete block: conventional tillage with crop residue removed (T), conventional tillage with residue incorporated into the soil (TS), no tillage with crop residue removed (NT), and no tillage with residue remaining on the soil surface (NTS). Soils were sampled in 2019 and microbial DNA was analyzed using high-throughput sequencing. After the 19-year (2001–2019) treatments, the soils with conservation tillage (NTS and NT) increased the soil’s microbial biomass carbon by 13%, organic carbon by 5%, and total nitrogen by 16% compared to conventional tillage (T and TS). The NTS treatment increased the abundance of the cbbL gene by 53% in the soil compared with the other soil treatments. The cbbL-carrying bacterial community was mainly affiliated with the phylum Proteobacteria and Actinobacteria, accounting for 56–85% of the community. Retaining crop residue in the field (NTS and TS) enhanced community-level physiological profiles by 31% and carbon substrate utilization by 32% compared to those without residue retention (T and NT). The 19 years of soil management lead to the conclusion that minimal soil disturbance, coupled with crop residue retention, shaped autotrophic bacterial phylogenetics, modified soil physicochemical properties, and created a microenvironment that favored CO₂-fixing activity and increased soil productivity. Full article

As one of the most important prototypical chromium-based MOFs, MIL-101(Cr) is well-studied and widely employed in various scientific fields. However, due to its small capture window sizes and curved internal apertures, its application in large molecular removal is quite limited, and given its high stability and high synthetic temperature (>200 °C), it is difficult to achieve hierarchically porous MIL-101(Cr). In our study, hierarchically porous MIL-101(Cr) involving a high macro-/meso-/micropores ratio was designed and synthesized using acetic acid as an additive and silicon dioxide (SiO₂) nanoparticles as a template. The optimal hierarchically porous MIL-101(Cr) (A-4) possessed a high specific surface area (2693 m² g⁻¹) and an abundant macro-/mesoporous structure with the addition of SiO₂ of 200 mg. Compared with the control sample (A-0) with a less macro-/mesoporous structure, A-4 showed good adsorption properties for both coomassie brilliant blue R-250 (CBB, 82.1 mg g⁻¹) and methylene blue (MB, 34.3 mg g⁻¹) dyes, which were 1.36 times and 9.37 times higher than those of A-0. Moreover, A-4 also had good recyclability, and the removal rate of CBB was still higher than 85% after five cycles of adsorption. Full article

The chloroplast protein CP12, which is widespread in photosynthetic organisms, belongs to the intrinsically disordered proteins family. This small protein (80 amino acid residues long) presents a bias in its composition; it is enriched in charged amino acids, has a small number of hydrophobic residues, and has a high proportion of disorder-promoting residues. More precisely, CP12 is a conditionally disordered proteins (CDP) dependent upon the redox state of its four cysteine residues. During the day, reducing conditions prevail in the chloroplast, and CP12 is fully disordered. Under oxidizing conditions (night), its cysteine residues form two disulfide bridges that confer some stability to some structural elements. Like many CDPs, CP12 plays key roles, and its redox-dependent conditional disorder is important for the main function of CP12: the dark/light regulation of the Calvin-Benson-Bassham (CBB) cycle responsible for CO₂ assimilation. Oxidized CP12 binds to glyceraldehyde-3-phosphate dehydrogenase and phosphoribulokinase and thereby inhibits their activity. However, recent studies reveal that CP12 may have other functions beyond the CBB cycle regulation. In this review, we report the discovery of this protein, its features as a disordered protein, and the many functions this small protein can have. Full article

In this work, new crosslinked pyridinium poly ionic liquid and its magnetite hybrid structured composite were prepared and applied to remove the toxic dye Coomassie Brilliant Blue (CBB-R250) from aqueous solutions. In this respect, vinyl pyridine, maleic anhydride, and dibromo nonane were used to prepare crosslinked quaternized vinyl pyridinium/maleic anhydride ionic liquid (CQVP-MA). Furthermore, a linear copolymer was prepared by the reaction of vinyl pyridine with bromo nonane followed by its copolymerization with maleic anhydride in order to use it as a capping agent for magnetite nanoparticles. The monodisperse MNPs were incorporated into the crosslinked PIL (CQVP-MA) by ultrasonication to prepare CQVP-MA/Fe₃O₄ composite to facilitate its recovery using an external magnetic field and enhance its adsorption capacity. The chemical structures, thermal stabilities, zeta potential, particle size, EDS, and SEM of the prepared CQVP-MA and CQVP-MA/Fe₃O₄ were investigated. Adsorption kinetics, isotherms, and mechanisms of CB-R250 elimination from aqueous solutions using CQVP-MA and CQVP-MA/Fe₃O₄ were also studied, and the results revealed that the pseudo second-order kinetic model and the Langmuir isotherm model were the most suitable to describe the CBB adsorption from an aqueous solution. The adsorption capacities of CQVP-MA and CQVP-MA/Fe₃O₄ were found to be 1040 and 1198, respectively, which are more than those for previously reported material in the literature with reasonable stability for five cycles. Full article

Nitrogen is essential for the biosynthesis of various molecules in cells, such as amino acids and nucleotides, as well as several types of lipids and sugars. Cyanobacteria can assimilate several forms of nitrogen, including nitrate, ammonium, and urea, and the physiological and genetic responses to these nitrogen sources have been studied previously. However, the metabolic changes in cyanobacteria caused by different nitrogen sources have not yet been characterized. This study aimed to elucidate the influence of nitrate and ammonium on the metabolic profiles of the cyanobacterium Synechocystis sp. strain PCC 6803. When supplemented with NaNO₃ or NH₄Cl as the nitrogen source, Synechocystis sp. PCC 6803 grew faster in NH₄Cl medium than in NaNO₃ medium. Metabolome analysis indicated that some metabolites in the CBB cycle, glycolysis, and TCA cycle, and amino acids were more abundant when grown in NH₄Cl medium than NaNO₃ medium. ¹⁵N turnover rate analysis revealed that the nitrogen assimilation rate in NH₄Cl medium was higher than in NaNO₃ medium. These results indicate that the mechanism of nitrogen assimilation in the GS-GOGAT cycle differs between NaNO₃ and NH₄Cl. We conclude that the amounts and biosynthetic rate of cyanobacterial metabolites varies depending on the type of nitrogen. Full article

Although engineered cyanobacteria for the production of lipids and fatty acids (FAs) are intelligently used as sustainable biofuel resources, intracellularly overproduced FAs disturb cellular homeostasis and eventually generate lethal toxicity. In order to improve their production by enhancing FFAs secretion into a medium, we constructed three engineered Synechocystis 6803 strains including KA (a mutant lacking the aas gene), KAOL (KA overexpressing lipA, encoding lipase A in membrane lipid hydrolysis), and KAOGR (KA overexpressing quadruple glpD/rbcLXS, related to the CBB cycle). Certain contents of intracellular lipids and secreted FFAs of all engineered strains were higher than those of the wild type. Remarkably, the KAOL strain attained the highest level of secreted FFAs by about 21.9%w/DCW at day 5 of normal BG₁₁ cultivation, with a higher growth rate and shorter doubling time. TEM images provided crucial evidence on the morphological changes of the KAOL strain, which accumulated abundant droplets on regions of thylakoid membranes throughout the cell when compared with wild type. On the other hand, BG₁₁-N condition significantly induced contents of both intracellular lipids and secreted FFAs of the KAOL strain up to 37.2 and 24.5%w/DCW, respectively, within 5 days. Then, for the first time, we shone a spotlight onto the overexpression of lipA in the aas mutant of Synechocystis as another potential strategy to achieve higher FFAs secretion with sustainable growth. Full article