Search Results (65)

In North America and Europe, asphalt shingle waste created during the installation of roofing membranes and tear-off shingles retrieved at the end of the membrane’s life cycle are two major sources of municipal solid waste. Since almost 15–35% of recycled asphalt shingles (RAS) consist of an asphalt binder, the effective recycling of RAS into asphalt mixtures could also allow a reduction in the consumption of non-renewable resources such as asphalt binders. In this context, several studies investigating the use of RAS in asphalt mixtures can be found in the literature, although they exhibit widespread and sometimes conflicting information about the investigated materials, the mix preparation and testing methodologies and the experimental findings. Given this background, this review paper aims at summarizing the existing information and research gaps, providing a synthetic and rational picture of the current literature, where similar attempts cannot be found. In particular, different research studies show that the use of RAS in asphalt mixtures is an economical as well as an eco-friendly option. RAS with up to 20% by weight of binder or 5% by weight of aggregate/mixtures (eventually in combination with 15% reclaimed asphalt pavement aggregate) were found to be relatively suitable to improve the performance properties of asphalt mixtures, both in the laboratory and in the field. Adding RAS to asphalt mixtures could enhance their stiffness, strength and rutting resistance (i.e., high-temperature properties), while negatively affecting the mixtures’ fatigue and thermal cracking resistance. However, the addition of specific biomaterials (e.g., bio-binders, bio-oils) or additives to asphalt mixtures can mitigate such issues, resulting in lower brittleness and shear susceptibilities and thus improving the anti-cracking performance. On the other hand, the literature review revealed that several aspects still need to be studied in detail. As an example, RAS-modified porous asphalt mixtures (fatigue, rutting, moisture susceptibility and thermal cracking) need specific research, and there are no comprehensive research studies on the effects of the RAS mixing time, size and mixing temperature in asphalt mixtures. Moreover, the addition of waste cooking/engine oils (biomaterials) as asphalt binder rejuvenators in combination with RAS represents an attractive aspect to be studied in detail. Full article

A 60-day feeding experiment was conducted to examine whether (i) soybean meal (SBM) protein in the diet of Nile tilapia (Oreochromis niloticus) can be replaced with protein from spent brewer’s yeast (SBY); (ii) co-rearing with biofloc alters fish growth, feed conversion and protein efficiency compared with rearing in clear water; and (iii) accumulated protein quantity and quality in biofloc acts as a possible feed source for the fish in periods of low feed intake. The fish were reared in either a bio-recirculating aquaculture system (Bio-RAS) or a clear-water RAS (Cw-RAS). In Bio-RAS, the mechanical and biological filters used in Cw-RAS were replaced with an open bioreactor that delivered heterotrophic-based biofloc to the rearing tanks and also acted as a sedimentation trap for effluent water before recirculating it back into the rearing unit. The fish were fed four iso-nitrogenous and iso-energetic diets (~28% crude protein, ~19 MJ kg⁻¹ gross energy) in which SBM protein was replaced with increasing levels of SBY, with triplicate tanks per inclusion level. The results revealed that average fish growth was greater in a biofloc environment compared with clear water and also greater at higher inclusion levels of SBY. However, in both rearing environments, fish growth displayed a second-degree polynomial distribution with increasing SBY inclusion level, with a peak between 30% and 60% inclusion. Fish in the biofloc environment showed better feed conversion ratio and protein retention, likely through ingesting both given feed and biofloc. Biofloc contained a significant amount of accumulated protein with a high biological profile, thereby constituting a possible feed reserve for the fish. A conclusion underlined by the apparent improved feed conversion of Bio-RAS reared fish, where that ingestion of biofloc will reduce the need for external feed per unit growth. Full article

The substitution of virgin asphalt binder with reclaimed asphalt pavement (RAP) has environmental and economic merits, however, cracking susceptibility arises due to the aged asphalt binder within RAP. The objectives of this study are to (1) enhance the cracking resistance of asphalt mixtures containing 50% RAP utilizing recycling agents (RAs) derived from six petroleum-based and bio-based materials, (2) conduct an environmental impact assessment (represented by global warming potential “GWP”) for high-RAP mixtures including RAs, and (3) estimate the cost effectiveness of including high-RAP content in asphalt mixtures. Based on the RAP asphalt binder performance grade (PG), base asphalt binder PG, and RAP content, the RA contents were determined to achieve a target asphalt binder of PG 76-22. A control mixture was benchmarked for comparison, specified for high-traffic volume roads, and contained PG 76-22 polymer-modified asphalt binder. The engineering performance of studied asphalt mixtures was evaluated using the Hamburg wheel-tracking (HWT), semi-circular bend, Illinois flexibility index, Ideal cracking tolerance, and thermal stress-restrained specimen tensile strength tests. It was found that petroleum-derived aromatic oil, soy-based oil, and tall oil fatty acid-based RAs demonstrated a successful restoration of aged RAP asphalt binder without compromising the permanent deformation resistance. The 50% RAP mixtures emitted less GWP by 41% and 42.9% using petroleum- and bio-oil RAs, respectively, and achieved a 31% cost reduction compared to the control mixtures. Full article

The equilibrium swelling test was employed to determine the swelling response of Nitrile Butadiene Rubber (NBR) with various acrylonitrile (ACN) contents, and the three-dimensional solubility parameter (HSP) and modified Flory–Huggins interaction parameter (χ_HSP) were used to establish the prediction model of the oil-resistant property. The results indicate that the energy difference (Ra) between NBR and solvents calculated by HSP values can be correlated with the swelling response qualitatively with an inversed “S-shape”, and high swelling response occurs at Ra < 8 MPa^1/2 for NBR. For the purpose of establishing the prediction model, the new modified χ_HSP value has been calculated and fitted with the swelling response using exponential and logarithmic fittings, respectively. Two prediction models considering all the possible influencing factors have been obtained to determine the swelling response and oil resistance of NBR-based rubber products in bio-fuels, represented by the bio-diesel and IRM 903 test oil in this work. The swelling response of NBR can be evaluated precisely, and high swelling regions can be predicted and avoided in the new emerging fuels through the prediction models. Thus, the oil resistance of NBR-based rubber products, such as seals, holes and gaskets can be well predicted now. Full article

The two-kidney, one-clip (2K1C) Goldblatt rodent model elicits a reduction in renal blood flow (RBF) in the clipped kidney (CK). The reduced RBF and oxygen bio-ability causes the accumulation of the tricarboxylic cycle intermediary, α-ketoglutarate, which activates the oxoglutarate receptor-1 (OXGR1). In the kidney, OXGR1 is abundantly expressed in intercalated cells (ICs) of the collecting duct (CD), thus contributing to sodium transport and electrolyte balance. The (pro)renin receptor (PRR), a member of the renin–angiotensin system (RAS), is a key regulator of sodium reabsorption and blood pressure (BP) that is expressed in ICs. The PRR is upregulated in 2K1C rats. Here, we tested the hypothesis that chronic reduction in RBF in the CK leads to OXGR1-dependent PRR upregulation in the CD and alters sodium balance and BP in 2K1C mice. To determine the role of OXGR1 in regulating the PRR in the CDs during renovascular hypertension, we performed 2K1C Goldblatt surgery (clip = 0.13 mm internal gap, 14 days) in two groups of male mice: (1) mice treated with Montelukast (OXGR1 antagonist; 5 mg/Kg/day); (2) OXGR1^−/− knockout mice. Wild-type and sham-operated mice were used as controls. After 14 days, 2K1C mice showed increased systolic BP (SBP) (108 ± 11 vs. control 82 ± 5 mmHg, p < 0.01) and a lower natriuretic response after the saline challenge test. The CK group showed upregulation of erythropoietin, augmented α-ketoglutarate, and increased PRR expression in the renal medulla. The CK of OXGR1 knockout mice and mice subjected to the OXGR1 antagonist elicited impaired PRR upregulation, attenuated SBP, and better natriuretic responses. In 2K1C mice, the effect of reduced RBF on the OXGR1-dependent PRR upregulation in the CK may contribute to the anti-natriuretic and increased SBP responses. Full article

(1) Background: The adhesion and microbiological behaviour of thermoplastic PETG dental appliance surfaces is governed by roughness parameters. The aim of this research was to evaluate the antibiofilm activity of alkaline peroxide-based disinfectant in Candida albicans biofilms on thermoplastic PETG, related to artificial ageing and surface characteristics, on multiscale levels. (2) Methods: In the present study, two PETG materials were investigated: Crystal^® (Bio Art Dental Equipment, Sao Carlos, Brazil), noted as C, and Duran^® (Scheu-Dental GmbH, Iserlohn, Germany)—noted as D. Half of the specimens were thermally cycled (TC), resulting in four sample groups, as follows: C, CTC, D, and DTC. Surface roughness was evaluated on different scale topographies. The biofilms were grown on the surfaces. An alkaline peroxide-based disinfectant was used. Statistical analyses were performed. (3) Results: Related to nanoroughness, there are insignificant differences among materials or related to thermocycling. More irregular surfaces are associated with larger grain sizes. After thermocycling, micro-roughness values increase. Disinfectant activity decreases the amount of biofilm developed on the surfaces, significantly in the two groups, but is not correlated to the material and artificial ageing. (4) Conclusion: The impact of surface roughness (Ra) on biofilm constitution is controlled by different scale topographies. Full article

Approximately 90% of patients with arthritis exhibit forefoot deformities, including deformities within the metatarsophalangeal and proximal interphalangeal joints. Current pharmacological treatment with Disease Modifying Antirheumatic Drugs (DMARDs) consists of two groups: synthetic drugs (sDMARDs) and biological drugs (bDMARDs). The objective of our study was to investigate foot anthropometry changes in RA patients based on the administered treatment over a five-year period Method: A longitudinal analysis was conducted with RA patients who were grouped based on their pharmacological treatment. The pharmacological treatment groups were categorized into (I) methotrexate (MTX), (II) MTX plus biological treatments (including all variables), (III) biological treatment alone, and (IV) a miscellaneous group comprising patients with diverse treatments, including patients for whom various drugs had failed or who had not achieved remission with pharmacological treatment. For the anthropometric measurements, a foot measurement platform validated by McPoil et al. was used. Post hoc analyses with Bonferroni correction were performed to identify pairwise differences between the treatment groups while controlling for Type I errors due to multiple comparisons. Results: In the period from 2018 to 2023, significant changes were observed in several foot measurements. For instance, the MTX group showed a statistically significant increase in left heel width (p = 0.026). The MTX group experienced a slight increase in left foot length, while the Biologics and MTX + Bio groups exhibited more substantial increases in both maximum medial arch height and midfoot width. Conclusions: Different RA treatments can have a significant impact on foot structure over a five-year period, showing notable changes in heel width and overall foot morphology. Combined treatments with MTX and biologics potentially offer better management of RA. Full article

Industry 4.0 integrates the intelligent networking of machines and processes through advanced information and communication technologies (ICTs). Despite advancements, small mechanical manufacturing enterprises face significant challenges transitioning to ICT-supported Industry 4.0 models due to a lack of technical expertise and infrastructure. These enterprises commonly encounter variable production volumes, differing priorities in customer orders, and diverse production capacities across low-, medium-, and high-level outputs. Frequent issues with machine health, glitches, and major breakdowns further complicate optimizing production scheduling. This paper presents a novel production management approach that harnesses bio-inspired methods alongside Internet of Things (IoT) technology to address these challenges. This comprehensive approach integrates the real-time monitoring and intelligent production order distribution, leveraging advanced LoRa wireless communication technology. The system ensures efficient and concurrent data acquisition from multiple sensors, facilitating accurate and prompt capture, transmission, and storage of machine status data. The experimental results demonstrate significant improvements in data collection time and system responsiveness, enabling the timely detection and resolution of machine failures. Additionally, an enhanced genetic algorithm dynamically allocates tasks based on machine status, effectively reducing production completion time and machine idle time. Case studies in a screw manufacturing facility validate the practical applicability and effectiveness of the proposed system. The seamless integration of the scheduling algorithm with the real-time monitoring subsystem ensures a coordinated and efficient production process, ultimately enhancing productivity and resource utilization. The proposed system’s robustness and efficiency highlight its potential to revolutionize production management in small-scale manufacturing settings. Full article

The lockdown imposed to combat the COVID-19 pandemic produced a historic fall in air pollution in cities like Barcelona. This exceptional situation offered a unique context in which to examine the effects of air pollutants on human health. The present study aims to determine and compare the oxidative stress biomarkers Th1/Th2 and inflammatory-related cytokines in healthy individuals first during lockdown and then six months after the easing of the restrictions on mobility. A prospective study of a representative sample of 58 healthy, non-smoking adults was carried out. During lockdown and six months post-easing of restrictions, blood samples were drawn to measure the percentage of eosinophils, levels of Th1/Th2 and inflammatory-related cytokines assessed by a multiplex assay (BioRad Laboratories S.A., Marnes-la-Coquette, France), and levels of 8-isoprostane, glutathione peroxidase activity, and myeloperoxidase (Cayman Chemical Co., Ann Arbor, MI, USA), to assess their value as biomarkers of oxidative stress. Six months after easing mobility restrictions, increases in the levels of 8-isoprostane (p < 0.0001), IL-1β (p = 0.0013), IL-1ra (p = 0.0110), IL-4 (p < 0.0001), IL-13 (p < 0.0001), G-CSF (p = 0.0007), and CCL3 (p < 0.0001) were recorded, along with reductions in glutathione peroxidase (p < 0.0001), IFN-γ (p = 0.0145), TNFα (p < 0.0001), IP-10 (p < 0.0001), IL-2 (p < 0.0001), IL-7 (p < 0.0001), basic FGF (p < 0.0001), CCL4 (p < 0.0001), and CCL5 (p < 0.0001). No significant differences were observed in the rest of the biomarkers analyzed. The reduction in environmental pollution during the COVID-19 lockdown significantly lowered the levels of oxidative stress, systemic inflammation, and Th2-related cytokines in healthy people. Full article

Background: The Hugo™ Robot-Assisted Surgery (RAS) system is a new cutting-edge robotic platform designed for clinical applications. Nevertheless, its application for cystic renal tumors has not yet been thoroughly investigated. In this context, we present an initial series of Robot-Assisted Partial Nephrectomy (RAPN) procedures carried out using the Hugo™ RAS system for cystic renal masses. Methods: Between October 2022 and January 2024, twenty-seven RAPN procedures for renal tumors were performed at Fondazione Policlinico Universitario Campus Bio-Medico. Our prospective board-approved dataset was queried for “cystic features” (n = 12). Perioperative data were collected. The eGFR was calculated according to the CKD-EPI formula. Post-operative complications were reported according to the Clavien–Dindo classification. Computed tomography (CT) scans for follow-up were performed according to the EAU guidelines. Trifecta was defined as the coexistence of negative surgical margin status, no Clavien–Dindo grade ≥ 3 complications, and eGFR decline ≤ 30%. Results: All the patients successfully underwent RAPN without the need for conversion or additional port placement. The median docking and console time were 5.5 (IQR, 4–6) and 79.5 min (IQR, 58–91 min), respectively. No intraoperative complications occurred, as well as clashes between instruments or with the bedside assistant. Two minor postoperative complications were recorded (Clavien–Dindo II). At discharge, serum creatinine and eGFR were comparable to preoperative values. Only one patient (8.4%) displayed positive surgical margins. The rate of trifecta achievement was 91.7%. Conclusions: RAPN for cystic renal masses using the novel Hugo™ RAS system can be safely and effectively performed. This robotic system provided satisfactory peri-operative outcomes, preserving renal function and displaying low postoperative complications and a high trifecta rate achievement. Full article

(1) Background: Patients with Fuchs’ endothelial corneal dystrophy (FECD) may have coexisting cataracts and, therefore, may require a cataract surgery, which poses challenges due to potential endothelial cell damage. FECD is a degenerative eye disease of unclear etiology, with inflammatory cytokines maybe playing an important role in its development and progression. The present study aimed to investigate the cytokine profile in the aqueous humor of FECD eyes with cataract. (2) Methods: Fifty-two patients were included in the study, 26 with FECD + cataract and 26 with cataract as a control group. Samples of the aqueous humor were analyzed for pro- and anti-inflammatory cytokines using a Bio-Plex 200 system. (3) Results: Interleukin 1 receptor antagonist (IL-1Ra) and interleukin IL-8 levels were significantly higher in the aqueous humor of FECD + cataract patients compared to the control/cataract group. Moreover, the levels of anti-inflammatory IL-10 showed a strong trend to be higher in the FECD + cataract group compared to the control group. In contrast, there were no statistically significant differences in IL-1β, IL-6, IL-4, IL-10, IL-13, IL-17A, and tumor necrosis factor TNF-α between the groups. (4) Conclusions: Presented research contributes to a better understanding of FECD pathogenesis. Elevated levels of IL-1Ra and IL-8 may serve as a defense mechanism in people with FECD and coexisting cataract. Full article

A gaseous elemental mercury (Hg⁰) sampler was developed for the assessment of mercury (Hg) pollution from the air and utilised aluminium (Al) powder as the accumulation medium. The Hg sampler is presented as an alternative cost-effective sorbent that can be used for the assessment of Hg pollution in atmospheric air in areas where natural bio-indicators such as lichens and moss do not grow, including the urban environments. The chemical treatment of Al materials was necessary to weaken the aluminium oxide (Al₂O₃) layer to increase the adsorption capability of Al material. Treated Al samples were exposed to Hg vapours for one hour to two weeks in a Hg atmosphere chamber. Other Al powder samples were exposed to the ambient air at areas of the Tshwane Metropolitan Municipality for six to ten months. The analysis of samples by an RA-915+ Zeeman mercury analyser showed that the limit of detection (LOD) and limit of quantification (LOQ) for the determination of Hg in Al powder with a mass of 100 mg were found to be 0.31 ng g⁻¹ and 1.0 ng g⁻¹, respectively. The content of Hg that accumulated on Al powder was linear from 0.1 to 25 ng g⁻¹, thus enabling the measurement of Hg accumulation from air at the global average concentration level. Mercury from air that accumulated on Al powder in the Tshwane Metropolitan Municipality ranged between 70 ng g⁻¹ and 155 ng g⁻¹. Full article

Reclaimed asphalt pavement (RAP) has been utilized as a potential partial substitute for virgin asphalt binder in asphalt mixtures. However, a primary concern with increasing RAP content in asphalt mixtures is the cracking potential, attributed to the aged RAP asphalt binder (RAP-binder). To address this, the use of petroleum-based and bio-derived recycling agents (RAs) in enhancing the cracking resistance of high-RAP asphalt mixtures has been explored. The objective of this study is to ascertain the effectiveness of six RAs in mitigating cracking in high-RAP asphalt mixtures. The RAs considered include petroleum-crude-oil-derived aromatic oil, soy oil, and four types of tall-oil-derived phytosterol (industrial by-product, intermediate, purified, and fatty acid-based). The RAs’ dosages were optimized, based on RAP-binder and unmodified asphalt binder properties, to produce target PG 70-22 asphalt binder when incorporated in asphalt mixtures containing 30% RAP. To assess the engineering performance of these 30%-RAP asphalt mixtures for each RA, a conventional asphalt mixture incorporating styrene-butadiene-styrene (SBS)-modified PG 70-22 asphalt binder without RAP or RAs was benchmarked for comparison. Mechanical tests performed included Hamburg wheel-track testing (HWTT), intermediate-temperature fracture tests (semi-circular bend, Illinois flexibility index, and IDEAL cracking tolerance), and thermal stress-restrained specimen tensile strength test to evaluate permanent deformation, intermediate-temperature cracking resistance, and low-temperature cracking resistance, respectively. Results showed that petroleum-crude-oil-derived aromatic oil and tall-oil-derived fatty-acid-based oil RAs were able to rejuvenate RAP-binder as measured by the cracking tests performed. Further, the use of these RAs did not adversely impact the asphalt mixtures’ permanent deformation performance. Full article

Currently, approximately 90% of road pavement mixtures are derived from fossil fuels, a major source of the greenhouse gases contributing to global warming. This fact, together with the scarcity of raw materials in pavement engineering, has prompted recent investigations into sustainable alternatives. Biobinders, serving as substitutes or modifiers for petroleum-based asphalt binders, have gained attention, alongside the integration of recycled materials in recycled asphalt (RA). This study addresses these concerns by combining three techniques: (1) substituting a bitumen-based binder with a biobinder; (2) incorporating a high RA percentage (>30%); and (3) manufacturing the asphalt mixture at a reduced temperature (140 °C). These approaches result in the production and evaluation of warm bio-recycled asphalt mixtures. Materials were assessed at both the binder and mixture levels. The control binder, RA binder, and biobinder underwent conventional and rheological characterization. In terms of mixtures, warm bio-recycled asphalt mixtures employed a biobinder as the only virgin binder, with RA contents of 50% and 70%. Mechanical characterization focused on bearing capacity, cohesion, permanent deformations, and moisture damage. The warm bio-recycled asphalt mixtures exhibited adequate outcomes in bearing capacity through the stiffness modulus being 18,120 MPa and 15,683 MPa for bio-recycled asphalt with 50% RA and bio-recycled asphalt with 70% RA, respectively. Bio-recycled asphalt with 50% RA and bio-recycled asphalt with 70% RA showed low permanent deformation percentages, specifically 0.5% and 0.7%, respectively, in comparison to the reference recycled asphalt mixture with 1.5%, allaying concerns in practical applications due to the biobinder’s soft consistency. The bio-recycled asphalt mixture with 70% RA displayed good mechanical performance regarding the studied mechanical characterization, especially exhibiting the least susceptibility to water-induced damage with 97% of the retained indirect tensile strength ratio, addressing concerns related to moisture damage in warm asphalt mixtures with high RA content and biobinders. These findings offer valuable insights into the adoption of more sustainable practices in the asphalt pavement industry, reducing the concerns associated with warm bio-recycled asphalt mixtures. Full article

Thermoplastic polyurethane (TPU) is a polymer used in a variety of fields, including medical applications. Here, we aimed to verify if the brush and bar coater deposition techniques did not alter TPU properties. The topography of the TPU-modified surfaces was studied via AFM demonstrating no significant differences between brush and bar coater-modified surfaces, compared to the un-modified TPU (TPU Film). The effect of the surfaces on planktonic bacteria, evaluated by MTT assay, demonstrated their anti-adhesive effect on E. coli, while the bar coater significantly reduced staphylococcal planktonic adhesion and both bacterial biofilms compared to other samples. Interestingly, Pearson’s R coefficient analysis showed that R_a roughness and Haralick’s correlation feature were trend predictors for planktonic bacterial cells adhesion. The surface adhesion property was evaluated against NIH-3T3 murine fibroblasts by MTT and against human fibrinogen and human platelet-rich plasma by ELISA and LDH assay, respectively. An indirect cytotoxicity experiment against NIH-3T3 confirmed the biocompatibility of the TPUs. Overall, the results indicated that the deposition techniques did not alter the antibacterial and anti-adhesive surface properties of modified TPU compared to un-modified TPU, nor its bio- and hemocompatibility, confirming the suitability of TPU brush and bar coater films in the biomedical and pharmaceutical fields. Full article