Investigating the Inhibitory Effect of Lactic Acid on Biofilm Production by Raw Chicken Meat Campylobacter spp. Isolates in Pure and Mixed Cultures †
Abstract
:1. Introduction
2. Methods
2.1. Preparation of Sterile Chicken Juice (CJ)
2.2. Campylobacter Isolates and Preparation of Their Working Cultures
2.3. Determination of Minimum Inhibitory and Bactericidal Concentrations (MICs and MBCs) of LA against Planktonic Campylobacter Bacteria
2.4. Determination of Minimum Biofilm Inhibitory Concentrations (MBICs) of LA against Single and Mixed Campylobacter Cultures
2.4.1. Determination of MBICs of LA against Single Cultures
2.4.2. Determination of MBICs of LA against Mixed Cultures
2.5. The Inhibitory Effect of LA on Biofilm Production by a Mixed Campylobacter Culture on PS and SS Surfaces
3. Results and Discussion
4. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Isolate Code | Species | Other Information | Poultry Isolation Origin |
---|---|---|---|
CAMP 1_005 | C. coli | strong BP 2, MDR 3 | wings |
CAMP_022 | C. jejuni | strong BP, MDR | minced meat |
CAMP_025 | C. coli | strong BP, MDR | neck |
CAMP_048 | C. jejuni | strong BP | souvlaki |
CAMP_083 | C. coli | weak BP, high resistance to ERY 4 | thigh |
CAMP_091 | C. jejuni | weak BP, high resistance to ERY | wings |
CAMP_114 | C. jejuni | moderate BP, MDR | neck |
CAMP_130 | C. jejuni | weak BP, MDR | wings |
Consortium Code | Group A 1 | Group B 2 | Group C 3 |
---|---|---|---|
CONS1 | CAMP_130 | CAMP_083 | CAMP_048 |
CONS2 | CAMP_130 | CAMP_091 | CAMP_022 |
CONS3 | CAMP_130 | CAMP_083 | CAMP_005 |
Campylobacter/Consortium Code | MIC 2 | MBC 3 | MIC | MBC | MBIC 4 | |
---|---|---|---|---|---|---|
Species/Isolates | μg/mL | |||||
in MH 5 | in MH -HB 6 | in MH -CJ 7 | ||||
CAMP 1_005 | C. coli | 1024 | 1024 | 2048 | 2048 | 1024 |
CAMP_022 | C. jejuni | 1024 | 1024 | 2048 | 2048 | 1024 |
CAMP_025 | C. coli | 1024 | 1024 | 1024 | 1024 | 1024 |
CAMP_048 | C. jejuni | 2048 | 2048 | 2048 | 2048 | 1024 |
CAMP_083 | C. coli | 1024 | 2048 | 2048 | 2048 | 2048 |
CAMP_091 | C. jejuni | 2048 | 2048 | 2048 | 2048 | 1024 |
CAMP_114 | C. jejuni | 1024 | 1024 | 2048 | 2048 | 1024 |
CAMP_130 | C. jejuni | 1024 | 1024 | 2048 | 2048 | 2048 |
CONS1 | CAMP_048/083/130 | 4096 | ||||
CONS2 | CAMP_022/091/130 | 4096 | ||||
CONS3 | CAMP_005/083/130 | 4096 |
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Kostoglou, D.; Vass, A.; Giaouris, E. Investigating the Inhibitory Effect of Lactic Acid on Biofilm Production by Raw Chicken Meat Campylobacter spp. Isolates in Pure and Mixed Cultures. Biol. Life Sci. Forum 2023, 26, 45. https://doi.org/10.3390/Foods2023-15078
Kostoglou D, Vass A, Giaouris E. Investigating the Inhibitory Effect of Lactic Acid on Biofilm Production by Raw Chicken Meat Campylobacter spp. Isolates in Pure and Mixed Cultures. Biology and Life Sciences Forum. 2023; 26(1):45. https://doi.org/10.3390/Foods2023-15078
Chicago/Turabian StyleKostoglou, Dimitra, Athina Vass, and Efstathios Giaouris. 2023. "Investigating the Inhibitory Effect of Lactic Acid on Biofilm Production by Raw Chicken Meat Campylobacter spp. Isolates in Pure and Mixed Cultures" Biology and Life Sciences Forum 26, no. 1: 45. https://doi.org/10.3390/Foods2023-15078