Coriolopsis trogii MUT3379: A Novel Cell Factory for High-Yield Laccase Production
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strain, Reagents, and Culture Media
2.2. Cultivation of C. trogii MUT3379 and Optimization of Laccase Production
2.3. Laccase Production Monitoring
2.4. Laccase Production and Purification
2.5. Laccase Identification by Tandem Mass Spectrometry
2.6. Substrate Range
2.7. Kinetic Properties
3. Results
3.1. Fermentation Process Development
3.2. Laccase Purification
3.3. Identification of Lac3379-1 by Proteomic Analysis
3.4. Lac3379-1 Characterization
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Purification Step | Volume (mL) | Total Activity (U) | Total Protein (mg) | Specific Activity (U mg−1) | Yield (%) | Purification Fold |
---|---|---|---|---|---|---|
Culture filtrate | 60 | 13,700 | 61 | 226 | 10 | 1 |
(NH4)2SO4 precipitation | 6 | 11,200 | 37 | 303.4 | 82 | 1.3 |
Ion-exchange chromatography | 6 | 10,900 | 22 | 494.3 | 79 | 2.2 |
Substrate | Km (μM) | kcat (s−1) | kcat/Km (s−1 μM−1) |
---|---|---|---|
ABTS | 2.6 ± 0.6 | 154.1 ± 0.8 | 58.4 ± 13 |
2,6-DMP | 105.5 ± 8 | 27.5 ± 0.6 | 0.26 ± 0.02 |
K4Fe(CN)6 | 76.9 ± 14 | 2.2 ± 0.07 | 0.029 ± 0.004 |
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Mellere, L.; Bellasio, M.; Berini, F.; Marinelli, F.; Armengaud, J.; Beltrametti, F. Coriolopsis trogii MUT3379: A Novel Cell Factory for High-Yield Laccase Production. Fermentation 2024, 10, 376. https://doi.org/10.3390/fermentation10070376
Mellere L, Bellasio M, Berini F, Marinelli F, Armengaud J, Beltrametti F. Coriolopsis trogii MUT3379: A Novel Cell Factory for High-Yield Laccase Production. Fermentation. 2024; 10(7):376. https://doi.org/10.3390/fermentation10070376
Chicago/Turabian StyleMellere, Luca, Martina Bellasio, Francesca Berini, Flavia Marinelli, Jean Armengaud, and Fabrizio Beltrametti. 2024. "Coriolopsis trogii MUT3379: A Novel Cell Factory for High-Yield Laccase Production" Fermentation 10, no. 7: 376. https://doi.org/10.3390/fermentation10070376