Dehydrin CaDHN2 Enhances Drought Tolerance by Affecting Ascorbic Acid Synthesis under Drought in Peppers
Abstract
:1. Introduction
2. Results
2.1. CaDHN2 Is a SK3-Type Dehydrin
2.2. CaDHN2 Is Induced by Drought, Salt, and ABA
2.3. CaDHN2 Is Localized in the Plasma Membrane, Nucleus, and Cytoplasm
2.4. CaDHN2 Enhances Drought Tolerance in Capsicum annuum L.
2.5. The Overexpression of CaDHN2 in Arabidopsis Enhances Drought Tolerance
2.6. CaDHN2 Interacts with CaGGP1 Directly
2.7. CaDHN2 Protects CaGGP1 to Maintain AsA Synthesis under Drought Stress
3. Discussion
4. Material and Methods
4.1. Plant Materials
4.2. qRT-PCR
4.3. Stress Treatment
4.4. Electrolyte Leakage
4.5. Enzyme Activity
4.6. NBT and DAB Staining
4.7. Measurement of H2O2 and O2−
4.8. Biomolecular Fluorescence Complementation (BiFC)
4.9. GST Pull-Down
4.10. Yeast Two-Hybrid Assay
4.11. Ascorbic Acid Content Measurement
4.12. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Li, X.; Feng, H.; Liu, S.; Cui, J.; Liu, J.; Shi, M.; Zhao, J.; Wang, L. Dehydrin CaDHN2 Enhances Drought Tolerance by Affecting Ascorbic Acid Synthesis under Drought in Peppers. Plants 2023, 12, 3895. https://doi.org/10.3390/plants12223895
Li X, Feng H, Liu S, Cui J, Liu J, Shi M, Zhao J, Wang L. Dehydrin CaDHN2 Enhances Drought Tolerance by Affecting Ascorbic Acid Synthesis under Drought in Peppers. Plants. 2023; 12(22):3895. https://doi.org/10.3390/plants12223895
Chicago/Turabian StyleLi, Xin, Hao Feng, Sha Liu, Junjun Cui, Jiannan Liu, Mingyu Shi, Jielong Zhao, and Lihu Wang. 2023. "Dehydrin CaDHN2 Enhances Drought Tolerance by Affecting Ascorbic Acid Synthesis under Drought in Peppers" Plants 12, no. 22: 3895. https://doi.org/10.3390/plants12223895