Rhizospheric Actinomycetes Revealed Antifungal and Plant-Growth-Promoting Activities under Controlled Environment
Abstract
:1. Introduction
2. Results
2.1. Isolation and Preliminary Screening
2.2. Molecular Identification
2.3. Extracellular Hydrolytic Enzymes
2.4. In Vivo Growth Promoting and Disease Control
2.4.1. Eco-Physiological Parameters
2.4.2. Disease Control
3. Discussion
4. Materials and Methods
4.1. Soil Sampling and Isolation
4.2. Antagonistic Activity
4.3. Molecular Identification
4.4. Extracellular Hydrolytic Enzymes
4.5. In Vivo Growth Promoting Effect and Disease Control
4.6. Statistical Analysis
Primers | Sequences | Target | Amplified Fragment (kb) | Gene | Reference |
---|---|---|---|---|---|
Y1 | 5′-TGGCTCAGAACGAACGCTGGCGGC-3′ | Bacteria | 0.43 | 16S rDNA | Darrasse et al. [53] |
Y2 | 5′-CCCACTGCTGCCTCCCGTAGGAGT-3′ |
5. Conclusions
Author Contributions
Funding
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Isolates | Antagonistic Activity | |
---|---|---|
E. coli | B. megaterium | |
AC1 * | +++ | +++ |
AC2 | - | - |
AC3 | + | + |
RS1 | + | - |
RS2 | - | - |
RS3 * | +++ | +++ |
FG1 | + | ++ |
FG2 | + | - |
OL1 | + | - |
OL2 * | +++ | ++ |
Enzyme | Substrates | Staining | Diameter of Hydrolysis Area (mm) | ||
---|---|---|---|---|---|
AC1 Streptomyces sp. | RS3 S. atratus | OL2 A. humicola | |||
Chitinase | Chitin azure (1%) | Congo red (0.03%) | 23.0 ± 2.3 b | 0.0 ± 0.0 c | 31.5 ± 1.7 a |
Chitin crab shells (1%) | Congo red (0.03%) | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 | |
Amylase | Soluble starch (1%) | Lugol solution (a) | 37.5 ± 2.9 a | 42.0 ± 1.2 a | 28.0 ± 3.5 b |
Glucanase | Lichenan (0.2%) | Congo red (0.03%) | 22.0 ± 2.3 b | 0.0 ± 0.0 c | 36.0 ± 1.2 a |
Pectinase | Pectin (0.5%) | CTAB (b) (2%) | 14.0 ± 1.2 a | 10.5 ± 1.7 a | 0.0 ± 0.00 b |
Protease | Skim milk (1%) | - | 14.5 ± 2.9 b | 12.5 ± 2.9 b | 21.5 ± 1.7 a |
Polygalacturanase | Polygalacturonic acid (1%) | Ruthenium red (0.1%) | 0.0 ± 0.0 | 0.0 ± 0.0 | 0.0 ± 0.0 |
Actinomycetes Isolates | Eco-Physiological Parameters | ||||
---|---|---|---|---|---|
TN (n) | SL (cm) | LN (n) | SFW (g) | SDW (g) | |
Cont. -ve | 8 ± 1.4 a | 36.05 ± 3.2 ab | 116 ± 6.9 b | 150.02 ± 4.1 b | 15.33 ± 1.4 b |
AC1: Streptomyces sp. | 8 ± 0.9 a | 39.01 ± 4.1 a | 195 ± 11.8 a | 204.00 ± 13.4 a | 33.02 ± 4.5 a |
RS3: Streptomyces atratus | 6 ± 1.2 a | 38.25 ± 7.1 a | 123 ± 13.6 b | 119.33 ± 8.0 c | 15.78 ± 1.9 b |
OL2: Arthrobacter humicola | 7 ± 1.0 a | 46.00 ± 3.2 a | 151 ± 7.2 a | 184.01 ± 7.9 a | 24.76 ± 2.7 a |
Actinomycetes Isolates | Eco-Physiological Parameters | ||||
---|---|---|---|---|---|
TW (n) | SL (cm) | LN (n) | SFW (g) | SDW (g) | |
Cont. -ve | 5 ± 0.2 b | 42.32 ± 0.3 a | 161 ± 1.0 bc | 142.33 ± 1.0 c | 24.04 ± 0.2 ab |
AC1: Streptomyces sp. | 8 ± 0.1 a | 54.31 ± 0.4 a | 333 ± 2.3 a | 240.12 ± 2.5 a | 30.67 ± 0.3 a |
RS3: Streptomyces atratus | 5 ± 0.1 b | 44.34 ± 0.8 a | 210 ± 1.8 b | 214.67 ± 1.9 ab | 23.00 ± 1.0 ab |
OL2: Arthrobacter humicola | 8 ± 0.1 a | 51.76 ± 0.2 a | 477 ± 3.7 a | 304.65 ± 0.8 a | 29.67 ± 0.6 a |
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Elshafie, H.S.; Camele, I. Rhizospheric Actinomycetes Revealed Antifungal and Plant-Growth-Promoting Activities under Controlled Environment. Plants 2022, 11, 1872. https://doi.org/10.3390/plants11141872
Elshafie HS, Camele I. Rhizospheric Actinomycetes Revealed Antifungal and Plant-Growth-Promoting Activities under Controlled Environment. Plants. 2022; 11(14):1872. https://doi.org/10.3390/plants11141872
Chicago/Turabian StyleElshafie, Hazem S., and Ippolito Camele. 2022. "Rhizospheric Actinomycetes Revealed Antifungal and Plant-Growth-Promoting Activities under Controlled Environment" Plants 11, no. 14: 1872. https://doi.org/10.3390/plants11141872