Transcriptome and Metabolome Integrated Analysis of Two Ecotypes of Tetrastigma hemsleyanum Reveals Candidate Genes Involved in Chlorogenic Acid Accumulation
Abstract
:1. Introduction
2. Results
2.1. Transcriptome Analysis in RG and SG13 Leaves
2.2. Metabolic Differences in RG and SG13 Leaves
2.3. Integrated Analysis of Metabolites and Transcripts in the Phenylpropanoid and Flavonoid Biosynthesis Pathway
2.4. Validation of Expression of Genes Involved in Chlorogenic Acid Accumulation
3. Discussion
4. Materials and Methods
4.1. Plant Materials and Growth Conditions
4.2. RNA Isolation and Transcriptome Sequencing
4.3. Metabolic Profiling
4.4. Verification of Candidate Genes by Quantitative Real-Time PCR (qRT-PCR)
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Compounds | Class | RG | SG13 | VIP | Log2 (Fold_Change) |
---|---|---|---|---|---|
Quinic acid O-glucuronic acid | Quinate and its derivatives | 31,266,667 | 9 | 1.3688 | −21.73 |
Chlorogenic acid (3-O-Caffeoylquinic acid) | Quinate and its derivatives | 18,966,667 | 9 | 1.3688 | −21.01 |
Isovitexin 7-O-glucoside (Saponarin) | Others | 6,086,667 | 9 | 1.3688 | −19.37 |
C-hexosyl-apigenin O-pentoside | Flavone C-glycosides | 5,670,000 | 9 | 1.3688 | −19.26 |
C-hexosyl-apigenin O-hexosyl-O-hexoside | Flavone C-glycosides | 2,243,333 | 9 | 1.3687 | −17.93 |
Kaempferol 3-O-rhamnoside (Kaempferin) | Flavonol | 1,956,667 | 9 | 1.3687 | −17.73 |
p-Coumaroyl quinic acid O-glucuronic acid | Quinate and its derivatives | 9 | 300,000 | 1.3687 | 15.02 |
Chrysin 5-O-glucoside (Toringin) | Flavone | 98,967 | 9 | 1.3688 | −13.42 |
Tricin 4′-O-(syringyl alcohol) ether 5-O-hexoside | Flavonolignan | 29,900 | 9 | 1.3687 | −11.70 |
Acetyl tryptophan | Amino acid derivatives | 16,166,667 | 601,000 | 1.3688 | −4.75 |
Accession Number | Function Prediction | KEGG ID | RG Count | SG13 Count | Fold Change (log2FC) | |
---|---|---|---|---|---|---|
Transcripts up-regulated in RG | c96568.graph_c0 | ADT | K05359 | 144 | 41 | 1.58 |
c103381.graph_c0 | PAL | K10775 | 2866 | 805 | 1.63 | |
c29476.graph_c0 | 4CL | K01904 | 12 | 0 | 3.08 | |
c94054.graph_c0 | C3H | K09754 | 1166 | 350 | 1.46 | |
c100085.graph_c0 | CSE | K18368 | 864 | 334 | 1.14 | |
c100482.graph_c0 | HCT | K13065 | 4779 | 118 | 5.04 | |
c78409.graph_c0 | HCT | K13065 | 95 | 0 | 6.16 | |
c102511.graph_c0 | CCoAOMT | K00588 | 3104 | 1280 | 1.06 | |
c100230.graph_c0 | CHS | K00660 | 6157 | 1532 | 1.80 | |
c90950.graph_c0 | CHS | K00660 | 5452 | 225 | 4.32 | |
c102872.graph_c0 | CHI | K01859 | 995 | 341 | 1.31 | |
Index | Metabolite name | KEGG ID | RG Content | SG13 Content | Fold Change (Log2FC) | |
Metabolites up-regulated in RG | pme0398 | Chlorogenic acid | C00852 | 18,966,667 | 9 | 21.00704 |
pme0424 | trans-cinnamaldehyde | C00903 | 94,433 | 45,900 | 1.040802 | |
pme2979 | Pinocembrin | C09827 | 7690 | 9 | 9.738843 | |
pme3473 | Butin | C09614 | 516,000 | 114,000 | 2.178337 | |
pme2960 | Naringenin chalcone | C06561 | 1,218,667 | 225,667 | 2.433038 | |
pme0377 | Naringenin | C00509 | 1,182,333 | 209,133 | 2.499142 | |
pme0002 | Neohesperidin | C09806 | 487,000 | 95,667 | 2.347834 | |
pme0330 | Naringin | C09789 | 1,046,667 | 410,333 | 1.350934 | |
pme0371 | Prunin | C09099 | 3,910,000 | 1,950,000 | 1.003694 | |
pme0379 | Apigenin | C01477 | 750,000 | 102,367 | 2.873145 |
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Yin, S.; Cui, H.; Zhang, L.; Yan, J.; Qian, L.; Ruan, S. Transcriptome and Metabolome Integrated Analysis of Two Ecotypes of Tetrastigma hemsleyanum Reveals Candidate Genes Involved in Chlorogenic Acid Accumulation. Plants 2021, 10, 1288. https://doi.org/10.3390/plants10071288
Yin S, Cui H, Zhang L, Yan J, Qian L, Ruan S. Transcriptome and Metabolome Integrated Analysis of Two Ecotypes of Tetrastigma hemsleyanum Reveals Candidate Genes Involved in Chlorogenic Acid Accumulation. Plants. 2021; 10(7):1288. https://doi.org/10.3390/plants10071288
Chicago/Turabian StyleYin, Shuya, Hairui Cui, Le Zhang, Jianli Yan, Lihua Qian, and Songlin Ruan. 2021. "Transcriptome and Metabolome Integrated Analysis of Two Ecotypes of Tetrastigma hemsleyanum Reveals Candidate Genes Involved in Chlorogenic Acid Accumulation" Plants 10, no. 7: 1288. https://doi.org/10.3390/plants10071288