Investigating Development and Defense Systems in Early Reproductive Stages of Male and Female Gonads in Black Scorpionfish Scorpaena porcus (Linnaeus, 1758)
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Specimen Collection and Sample Processing
2.2. Tissue Preparation for Histology and Histochemistry
2.3. Identification of Piscidin-1 Ortholog Protein in Pterois Miles Through In Silico Prediction
2.4. Primary Antibody
2.5. Immunoperoxidase
2.6. Immunofluorescence
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Macroscopic Criteria | |
---|---|
M1 | The testis is smaller than a third of the body cavity and is slender and white in color. |
M2 | The testes, thin and white, occupy less than half the body cavity until they become roughly symmetrical, pinkish in color, and measure about half the length of the body cavity. |
F1 | The tiny, translucent, pinkish ovary is shorter than half of the body cavity. The naked eye is unable to see the eggs. |
F2 | The ovary is small, pinkish/reddish, and even translucent, occupying approximately half of the body cavity. Blood vessels are visible, but the eggs are not visible to the naked eye. |
TL (mm) | TW (g) | |||||||
---|---|---|---|---|---|---|---|---|
Min | Max | Mean | SD | Min | Max | Mean | SD | |
Male specimens in the M1 stage | 70 | 85 | 78.75 | 6.29 | 6.40 | 12.20 | 10.04 | 2.62 |
Male specimens in the M2 stage | 80 | 165 | 130 | 30.09 | 9.63 | 93.4 | 50.17 | 29.88 |
Female specimens in the F1 stage | 65 | 93 | 80 | 7.07 | 7.21 | 15.51 | 10.81 | 1.81 |
Female specimens in the F2 stage | 70 | 230 | 143.93 | 44.06 | 8.17 | 230.5 | 75.96 | 64.61 |
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Alesci, A.; Marino, S.; D’Iglio, C.; Morgante, S.; Miller, A.; Rigano, G.; Ferri, J.; Fernandes, J.M.O.; Capillo, G. Investigating Development and Defense Systems in Early Reproductive Stages of Male and Female Gonads in Black Scorpionfish Scorpaena porcus (Linnaeus, 1758). Biology 2024, 13, 587. https://doi.org/10.3390/biology13080587
Alesci A, Marino S, D’Iglio C, Morgante S, Miller A, Rigano G, Ferri J, Fernandes JMO, Capillo G. Investigating Development and Defense Systems in Early Reproductive Stages of Male and Female Gonads in Black Scorpionfish Scorpaena porcus (Linnaeus, 1758). Biology. 2024; 13(8):587. https://doi.org/10.3390/biology13080587
Chicago/Turabian StyleAlesci, Alessio, Sebastian Marino, Claudio D’Iglio, Silvana Morgante, Anthea Miller, Gabriele Rigano, Josipa Ferri, Jorge M. O. Fernandes, and Gioele Capillo. 2024. "Investigating Development and Defense Systems in Early Reproductive Stages of Male and Female Gonads in Black Scorpionfish Scorpaena porcus (Linnaeus, 1758)" Biology 13, no. 8: 587. https://doi.org/10.3390/biology13080587