Ag85a-S2 Activates cGAS-STING Signaling Pathway in Intestinal Mucosal Cells
Abstract
:1. Introduction
2. Materials and Methods
2.1. Preparation of Ag85a-S2, Ag85a, and S2
2.2. Cell Culture
2.3. cGAS Knockout Cell Line Construction
2.4. In Vitro Stimulation
2.5. Animal Immunization
2.6. siRNA Transfection
2.7. qPCR Assay
2.8. Western Blotting Assay
2.9. ELISA Assay
2.10. RNA Sequencing and Bioinformatics Analysis
2.11. Statistical Analysis
3. Results
3.1. Ag85a-S2 Upregulates the Key Components in the cGAS-STING Cascade
3.2. Generation of cGAS Knockout Cell Line
3.3. Depletion of cGAS-STING Signaling Impairs Immune Cytokines Secretion
3.4. Ag85a-S2 Triggers Cellular Immune Response
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Conflicts of Interest
References
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Dang, S.; Li, W.; Wen, S.; Song, Y.; Bai, M.; Li, S.; Chen, Z.; Zhai, J. Ag85a-S2 Activates cGAS-STING Signaling Pathway in Intestinal Mucosal Cells. Vaccines 2022, 10, 2170. https://doi.org/10.3390/vaccines10122170
Dang S, Li W, Wen S, Song Y, Bai M, Li S, Chen Z, Zhai J. Ag85a-S2 Activates cGAS-STING Signaling Pathway in Intestinal Mucosal Cells. Vaccines. 2022; 10(12):2170. https://doi.org/10.3390/vaccines10122170
Chicago/Turabian StyleDang, Sheng, Wanyang Li, Shubo Wen, Yang Song, Meirong Bai, Shuyan Li, Zeliang Chen, and Jingbo Zhai. 2022. "Ag85a-S2 Activates cGAS-STING Signaling Pathway in Intestinal Mucosal Cells" Vaccines 10, no. 12: 2170. https://doi.org/10.3390/vaccines10122170