Human Heme Oxygenase-1 Induced by Interleukin-6 via JAK/STAT3 Pathways Is a Tumor Suppressor Gene in Hepatoma Cells
Abstract
:1. Introduction
2. Material and Methods
2.1. Cell Culture and Chemicals
2.2. Expression Vectors and Overexpression
2.3. Immunoblot Assay
2.4. Detection of ROS with Flow Cytometer and Total ROS Analysis
2.5. RT-qPCR
2.6. Matrigel Invasion Assay
2.7. Cell Proliferation
2.8. Enzyme-Linked Immunosorbent Assay (ELISA)
2.9. Report Vector Constructs
2.10. Reporter Assays
2.11. Xenograft Animal Study
2.12. Statistical Analysis
3. Results
3.1. HO-1 Retards Cell Proliferation and Cell Invasion of Human Hepatoma Cells
3.2. Ectopic Overexpression of HO-1 Inhibits Tumorigenesis of HepG3 Cells
3.3. HO-1 Alleviates ROS Generation in Hep3B Cells
3.4. HO-1 Downregulates IL-6 Expression in Hep3B Cells
3.5. Ectopic IL-6 Overexpression or IL-6 Treatment Enhances HO-1 Expression
3.6. IL-6 Induces HO-1 Reporter Activity
3.7. IL-6-Induced HO-1 Reporter Activity Is Dependent on STAT3 Signaling
3.8. IL-6 induces HO-1 Expression via STAT3 Phosphorylation
3.9. AG490 and Luteolin Block Activation of IL-6 on HO-1 Expression
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
References
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Chiang, K.-C.; Chang, K.-S.; Hsu, S.-Y.; Sung, H.-C.; Feng, T.-H.; Chao, M.; Juang, H.-H. Human Heme Oxygenase-1 Induced by Interleukin-6 via JAK/STAT3 Pathways Is a Tumor Suppressor Gene in Hepatoma Cells. Antioxidants 2020, 9, 251. https://doi.org/10.3390/antiox9030251
Chiang K-C, Chang K-S, Hsu S-Y, Sung H-C, Feng T-H, Chao M, Juang H-H. Human Heme Oxygenase-1 Induced by Interleukin-6 via JAK/STAT3 Pathways Is a Tumor Suppressor Gene in Hepatoma Cells. Antioxidants. 2020; 9(3):251. https://doi.org/10.3390/antiox9030251
Chicago/Turabian StyleChiang, Kun-Chun, Kang-Shuo Chang, Shu-Yuan Hsu, Hsin-Ching Sung, Tsui-Hsia Feng, Mei Chao, and Horng-Heng Juang. 2020. "Human Heme Oxygenase-1 Induced by Interleukin-6 via JAK/STAT3 Pathways Is a Tumor Suppressor Gene in Hepatoma Cells" Antioxidants 9, no. 3: 251. https://doi.org/10.3390/antiox9030251