Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa
Abstract
:1. Introduction
2. Materials and Methods
2.1. Materials
2.2. Animals and Treatment
2.3. Testes and Epididymes Homogenates Preparation
2.4. Sperm Motility and DNA Oxidation Determinations
2.5. Testes Histological Analysis, and Sperm Count
2.6. SDS-PAGE and Immunoblotting
2.7. Statistical Analysis
3. Results
3.1. Low Sperm Motility and High DNA Oxidation Suggest Compromised Sperm Quality Due to Oxidative Stress
3.2. Lipid Peroxidation Increased in Caput and Cauda Epididymis of t-BHP Treated Male Rats
3.3. PRDX1 and PRDX6 Are Differentially Upregulated in Caput and Cauda Epididymis at Different Time Points
3.4. Catalase Expression Shows Trends of Increase, with Significant Individual Variation in the Epididymis
3.5. PRDXs, Catalase and Thioredoxin Expression Levels and Lipid Peroxidation Are Similar in Testis Despite the t-BHP Treatment
3.6. Reproductive Organs Weight, Spermatogenesis, and Sperm Production Were Not Affected by the t-BHP Treatment
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Wu, P.Y.; Scarlata, E.; O’Flaherty, C. Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa. Antioxidants 2020, 9, 170. https://doi.org/10.3390/antiox9020170
Wu PY, Scarlata E, O’Flaherty C. Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa. Antioxidants. 2020; 9(2):170. https://doi.org/10.3390/antiox9020170
Chicago/Turabian StyleWu, Pei You, Eleonora Scarlata, and Cristian O’Flaherty. 2020. "Long-Term Adverse Effects of Oxidative Stress on Rat Epididymis and Spermatozoa" Antioxidants 9, no. 2: 170. https://doi.org/10.3390/antiox9020170