Red Light Control of β-Carotene Isomerisation to 9-cis β-Carotene and Carotenoid Accumulation in Dunaliella salina
Abstract
:1. Introduction
2. Materials and Methods
2.1. Strains and Cultivation
2.2. Carotenoids Analysis
2.3. Statistical Analysis
3. Results
4. Discussion
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Appendix A
References
- Xu, Y.; Harvey, P.J. Carotenoid production by Dunaliella salina under red light. Antioxidants 2019, 8, 123. [Google Scholar] [CrossRef] [PubMed]
- Khoo, H.E.; Nagendra, P.K.; Kong, K.W.; Jiang, Y.; Ismail, A. Carotenoids and their isomers: Color pigments in fruits and vegetables. Molecules 2011, 16, 1710–1738. [Google Scholar] [CrossRef] [PubMed]
- Hashimoto, H.; Sugai, Y.; Uragami, C.; Gardiner, A.T.; Cogdell, R.J. Natural and artificial light-harvesting systems utilizing the functions of carotenoids. J. Photochem. Photobiol. C: Photochem. Rev. 2015, 25, 46–70. [Google Scholar] [CrossRef] [Green Version]
- Rodriguez-Concepcion, M.; Avalos, J.; Bonet, M.L.; Boronat, A.; Gomez-Gomez, L.; Hornero-Mendez, D.; Limon, M.C.; Meléndez-Martínez, A.J.; Olmedilla-Alonso, B.; Palou, A.; et al. A global perspective on carotenoids: Metabolism, biotechnology, and benefits for nutrition and health. Prog. Lipid Res. 2018, 70, 62–93. [Google Scholar] [CrossRef] [Green Version]
- Ben-Amotz, A.; Katz, A.; Avron, M. Accumulation of β-carotene in halotolerant algae: Purification and characterization of β-carotene-rich globules from Dunaliella Bardawil (Chlorophyceae). J. Phycol. 1982, 18, 529–537. [Google Scholar] [CrossRef]
- Ben-Amotz, A. Analysis of carotenoids with emphasis on 9-cis β-carotene in vegetables and fruits commonly consumed in Israel. Food Chem. 1998, 62, 515–520. [Google Scholar] [CrossRef]
- Ben-Amotz, A.; Shaish, A.; Avron, M. Mode of action of the massively accumulated β-carotene of Dunaliella bardawil in protecting the alga against damage by excess irradiation. Plant Physiol. 1989, 91, 1040–1043. [Google Scholar] [CrossRef]
- Lamers, P.P.; Janssen, M.; De Vos, R.C.H.; Bino, R.J.; Wijffels, R.H. Exploring and exploiting carotenoid accumulation in Dunaliella salina for cell-factory applications. Trends Biotechnol. 2008, 26, 631–638. [Google Scholar] [CrossRef]
- Jiménez, C.; Pick, U. Differential stereoisomer compositions of β, β-carotene in thylakoids and in pigment globules in Dunaliella. J. Plant Physiol. 1994, 143, 257–263. [Google Scholar] [CrossRef]
- Davidi, L.; Shimoni, E.; Khozin-Goldberg, I.; Zamir, A.; Pick, U. Origin of β-carotene-rich plastoglobuli in Dunaliella bardawil. Plant Physiol. 2014, 164, 2139–2156. [Google Scholar] [CrossRef]
- Davidi, L.; Levin, Y.; Ben-Dor, S.; Pick, U. Proteome analysis of cytoplasmatic and plastidic β-carotene lipid droplets in Dunaliella bardawil. Plant Physiol. 2015, 167, 60–79. [Google Scholar] [CrossRef]
- Levin, G.; Mokady, S. Antioxidant activity of 9-cis compared to all-trans β-carotene in vitro. Free Radic. Biol. Med. 1994, 17, 77–82. [Google Scholar] [CrossRef]
- Jaime, L.; Mendiola, J.A.; Ibáñez, E.; Martin-Alvarez, P.J.; Cifuentes, A.; Reglero, G.; Señoráns, F.J. Beta-carotene isomer composition of sub- and supercritical carbon dioxide extracts. Antioxidant activity measurement. J. Agric. Food Chem. 2007, 55, 10585–10590. [Google Scholar] [CrossRef]
- Greenberger, S.; Harats, D.; Salameh, F.; Lubish, T.; Harari, A.; Trau, H.; Shaish, A. 9-cis-rich β-carotene powder of the alga Dunaliella reduces the severity of chronic plaque psoriasis: A randomized, double-blind, placebo-controlled clinical trial. J. Am. Coll. Nutr. 2012, 31, 320–326. [Google Scholar] [CrossRef]
- Harari, A.; Abecassis, R.; Relevi, N.; Levi, Z.; Ben-Amotz, A.; Kamari, Y.; Harats, D.; Shaish, A. Prevention of atherosclerosis progression by 9-cis-β-carotene rich alga Dunaliella in apoE-deficient mice. Biomed. Res. Int. 2013, 2013. [Google Scholar] [CrossRef]
- Rotenstreich, Y.; Belkin, M.; Sadetzki, S.; Chetrit, A.; Ferman-Attar, G.; Sher, I.; Harari, A.; Shaish, A.; Harats, D. Treatment with 9-cis β-carotene-rich powder in patients with retinitis pigmentosa: A randomized crossover trial. JAMA Ophthalmol. 2013, 131, 985–992. [Google Scholar] [CrossRef]
- Zolberg Relevy, N.; Bechor, S.; Harari, A.; Ben-Amotz, A.; Kamari, Y.; Harats, D.; Shaish, A. The inhibition of macrophage foam cell formation by 9-cis β-carotene is driven by BCMO1 activity. PLoS ONE 2015, 10, e0115272. [Google Scholar] [CrossRef] [PubMed]
- Weinrich, T.; Xu, Y.; Wosu, C.; Harvey, P.J.; Jeffery, G. Mitochondrial Function, Mobility and Lifespan Are Improved in Drosophila melanogaster by Extracts of 9-cis-β-Carotene from Dunaliella salina. Marine Drugs 2019, 17, 279. [Google Scholar] [CrossRef]
- Sher, I.; Tzameret, A.; Peri-Chen, S.; Edelshtain, V.; Ioffe, M.; Sayer, A.; Buzhansky, L.; Gazit, E.; Rotenstreich, Y. Synthetic 9-cis-beta-carotene inhibits photoreceptor degeneration in cultures of eye cups from rpe65rd12 mouse model of retinoid cycle defect. Sci. Rep. 2018, 8, 6130. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Shaish, A.; Avron, M.; Ben-Amotz, A. Effect of inhibitors on the formation of stereoisomers in the biosynthesis of β-carotene in Dunaliella bardawil. Plant Cell Physiol. 1990, 31, 689–696. [Google Scholar]
- Davidi, L.; Pick, U. Novel 9-cis/all-trans β-carotene isomerases from plastidic oil bodies in Dunaliella bardawil catalyze the conversion of all-trans to 9-cis β-carotene. Plant Cell Rep. 2017, 36, 807–814. [Google Scholar] [CrossRef]
- Ben-Amotz, A. Effect of low temperature on the stereoisomer composition of β-carotene in the halotolerant alga Dunaliella bardawil (chlorophyta). J. Phycol. 1996, 32, 272–275. [Google Scholar] [CrossRef]
- Ben-Amotz, A.; Lers, A.; Avron, M. Stereoisomers of β-carotene and phytoene in the alga Dunaliella bardawil. Plant Physiol. 1988, 86, 1286–1291. [Google Scholar] [CrossRef]
- Ben-Amotz, A.; Avron, M. The wavelength dependence of massive carotene synthesis in Dunaliella bardawil (Chlorophyceae). J. Phycol. 1989, 25, 175–178. [Google Scholar] [CrossRef]
- Orset, S.C.; Young, A.J. Exposure to low irradiances favors the synthesis of 9-cis beta, beta-carotene in Dunaliella salina (Teod.). Plant Physiol. 2000, 122, 609–618. [Google Scholar] [CrossRef]
- Gómez, P.I.; González, M.A. The effect of temperature and irradiance on the growth and carotenogenic capacity of seven strains of Dunaliella salina (Chlorophyta) cultivated under laboratory conditions. Biol. Res. 2005, 38, 151–162. [Google Scholar] [CrossRef]
- Borowitzka, M.A. Algal growth media and sources of algal cultures. In Micro-algal Biotechnology, 1st ed.; Borowitzka, M.A., Borowitzka, L.J., Eds.; Cambridge University Press: Cambridge, UK, 1988; pp. 456–465. [Google Scholar]
- Jimenez, C.; Pick, U. Differential reactivity of β-carotene isomers from Dunaliella bardawil toward oxygen radicals. Plant Physiol. 1993, 101, 385–390. [Google Scholar] [CrossRef] [PubMed]
- Hansberg, W.; Aguirre, J. Hyperoxidant states cause microbial cell differentiation by cell isolation from dioxygen. J. Theor. Biol. 1990, 142, 201–221. [Google Scholar] [CrossRef]
- Yokthongwattana, K.; Jin, E.; Melis, A. Chloroplast acclimation, photodamage and repair reactions of Photosystem-II in the model green alga, Dunaliella salina. In The Alga Dunaliella Biodiversity, Physiology, Genomics and Biotechnology, 1st ed.; Ben-Amotz, A., Polle, E.W., Subba Rao, D.V., Eds.; CRC Press: Enfield, NH, USA, 2009; pp. 273–299. [Google Scholar]
- Erickson, E.; Wakao, S.; Niyogi, K.K. Light stress and photoprotection in Chlamydomonas reinhardtii. Plant J. 2015, 82, 449–465. [Google Scholar] [CrossRef] [PubMed]
- Legris, M.; Burgie, E.S.; Rojas, C.C.R.; Neme, M.; Wigge, P.A.; Vierstra, R.D.; Casal, J.J. Phytochrome B integrates light and temperature signals in Arabidopsis. Science 2016, 354, 897–900. [Google Scholar] [CrossRef] [PubMed]
- Viczián, A.; Klose, C.; Ádám, É.; Nagy, F. New insights of red light-induced development. Plant Cell Environ. 2017, 40, 2457–2468. [Google Scholar] [CrossRef] [PubMed] [Green Version]
- Welsch, R.; Beyer, P.; Hugueney, P.; Kleinig, H.; von Lintig, J. Regulation and activation of phytoene synthase, a key enzyme in carotenoid biosynthesis, during photomorphogenesis. Planta 2000, 211, 846–854. [Google Scholar] [CrossRef] [PubMed]
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Xu, Y.; Harvey, P.J. Red Light Control of β-Carotene Isomerisation to 9-cis β-Carotene and Carotenoid Accumulation in Dunaliella salina. Antioxidants 2019, 8, 148. https://doi.org/10.3390/antiox8050148
Xu Y, Harvey PJ. Red Light Control of β-Carotene Isomerisation to 9-cis β-Carotene and Carotenoid Accumulation in Dunaliella salina. Antioxidants. 2019; 8(5):148. https://doi.org/10.3390/antiox8050148
Chicago/Turabian StyleXu, Yanan, and Patricia J. Harvey. 2019. "Red Light Control of β-Carotene Isomerisation to 9-cis β-Carotene and Carotenoid Accumulation in Dunaliella salina" Antioxidants 8, no. 5: 148. https://doi.org/10.3390/antiox8050148