Inhibitor of FTO, Rhein, Restrains the Differentiation of Myoblasts and Delays Skeletal Muscle Regeneration
Abstract
:Simple Summary
Abstract
1. Introduction
2. Materials and Methods
2.1. Animals
2.2. Cell Culture and Cell Treatment
2.3. qRT-PCR
2.4. Immunofluorescence
2.5. Western Blotting
2.6. Hematoxylin and Eosin Staining and Tissue Immunofluorescence Staining
2.7. Detect m6A Levels
2.8. Statistical Analysis
3. Results
3.1. Dynamic Changes in m6A Erasers and m6A Levels during C2C12 Differentiation
3.2. Dynamic Changes of m6A Erasers, Writers and Readers, and m6A Levels during Skeletal Muscle Injury
3.3. Increase m6A Level by Inhibiting FTO Inhibit C2C12 Differentiation
3.4. Increase of m6A Level by Inhibiting FTO Delays the Regeneration of Skeletal Muscle after Injury
4. Discussion
5. Conclusions
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Gene Name | Forward Primer | Reverse Primer |
---|---|---|
MyoG | GAGACATCCCCCTATTTCTACCA | GCTCAGTCCGCTCATAGCC |
FTO | CCGAGTCGTCCGGACTTTAC | GGAAACCACGTCTGTGAGGT |
ALKBH5 | GACAACTACAAGGCGGGCA | CCAGAGGCATACAGGGAC |
METTL3 | GGGCACTTGGATTTAAGGAACC | CTTAGGGCCGCTAGAGGTAGG |
METTL14 | GAGCTGAGAGTGCGGATAGC | GCAGATGTATCATAGGAAGCCC |
WTAP | ATGGCACGGGATGAGTTAATTC | TTCCCTTAAACCAGTCACATCG |
YTHDF1 | ACAGTTACCCCTCGATGAGTG | GGTAGTGAGATACGGGATGGGA |
YTHDF2 | GAGCAGAGACCAAAAGGTCAAG | CTGTGGGCTCAAGTAAGGTTC |
YTHDF3 | GATCAGCCTATGCCATATCTGAC | CCCCTGGTTGACTAAAAACACC |
GAPDH | ATCACTGCCACCCAGAAGACT | CATGCCAGTGAGCTTCCCGTT |
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Li, R.; Cao, Y.; Wu, W.; Liu, H.; Xu, S. Inhibitor of FTO, Rhein, Restrains the Differentiation of Myoblasts and Delays Skeletal Muscle Regeneration. Animals 2024, 14, 2434. https://doi.org/10.3390/ani14162434
Li R, Cao Y, Wu W, Liu H, Xu S. Inhibitor of FTO, Rhein, Restrains the Differentiation of Myoblasts and Delays Skeletal Muscle Regeneration. Animals. 2024; 14(16):2434. https://doi.org/10.3390/ani14162434
Chicago/Turabian StyleLi, Rongyang, Yan Cao, Wangjun Wu, Honglin Liu, and Shiyong Xu. 2024. "Inhibitor of FTO, Rhein, Restrains the Differentiation of Myoblasts and Delays Skeletal Muscle Regeneration" Animals 14, no. 16: 2434. https://doi.org/10.3390/ani14162434