Harnessing the Diversity of Burkholderia spp. Prophages for Therapeutic Potential
Abstract
:1. Introduction
2. Materials and Methods
2.1. Bacterial Isolates and Induction of Prophage Release
2.2. Isolation of Phages and Host Range Testing
2.3. Whole Genome Sequencing and Analysis
2.4. EM Imaging
2.5. Statistical Analysis
3. Results
3.1. Prophage Induction and Isolation
3.2. Whole Genome Sequencing of Burkholderia spp. Clinical Isolates
3.3. Phage Host-Range Screening
3.4. Whole Genome Sequencing of Phages
3.5. Analysis of Prophage Carriage in Burkholderia spp. Clinical Isolates
4. Discussion
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Phage ID | Source Isolate | Source Species | Length (bp) | GC % | Predicted Genus 1 | NCBI Similar Phage 2 |
---|---|---|---|---|---|---|
BCC02/03/04 | DVT1180 | B. multivorans | 34,126 | 64.2 | Peduovirinae; Kisquinquevirus | Burkholderia Phage KS5 (GU911303.1) |
BCC05/06 | DVT1166 | B. multivorans | 30,957 | 62.8 | Peduoviridae; Duodecimduovirus | Burkholderia Phage phiE12-2 (NC_009236.1) |
BCC07 | DVT1155 | B. seminalis | 38,216 | 66.7 | Peduoviridae; Aptresvirus | Burkholderia Phage Mana (NC_055863.1) |
BCC08 | DVT1155 | B. seminalis | 28,709 | 63.7 | Peduoviridae; Kayeltresvirus | Burkholderia Phage KL3 (GU911304.1) |
Bch7 | Env 3 | - | 68,166 | 54.7 | Bcepfunavirus | Burkholderia Phage Maja (MT708549.1) |
DSMZ 107315 | Env 3 | - | 22,967 | 61.5 | Peduovirinae; Kisquattuordecimvirus | Burkholderia Phage FLC5 (NC_055722.1) |
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Nordstrom, H.R.; Griffith, M.P.; Rangachar Srinivasa, V.; Wallace, N.R.; Li, A.; Cooper, V.S.; Shields, R.K.; Van Tyne, D. Harnessing the Diversity of Burkholderia spp. Prophages for Therapeutic Potential. Cells 2024, 13, 428. https://doi.org/10.3390/cells13050428
Nordstrom HR, Griffith MP, Rangachar Srinivasa V, Wallace NR, Li A, Cooper VS, Shields RK, Van Tyne D. Harnessing the Diversity of Burkholderia spp. Prophages for Therapeutic Potential. Cells. 2024; 13(5):428. https://doi.org/10.3390/cells13050428
Chicago/Turabian StyleNordstrom, Hayley R., Marissa P. Griffith, Vatsala Rangachar Srinivasa, Nathan R. Wallace, Anna Li, Vaughn S. Cooper, Ryan K. Shields, and Daria Van Tyne. 2024. "Harnessing the Diversity of Burkholderia spp. Prophages for Therapeutic Potential" Cells 13, no. 5: 428. https://doi.org/10.3390/cells13050428