Paired Transcriptomic Analyses of Atheromatous and Control Vessels Reveal Novel Autophagy and Immunoregulatory Genes in Peripheral Artery Disease
Abstract
:1. Introduction
2. Materials and Methods
2.1. Sample Collection and Processing
2.2. RNA Isolation and Quantitative Real-Time PCR
2.3. RNA Sequencing and Data Analysis
2.4. Differential Gene Expression Calculation
2.5. Tissue Processing and Staining
2.6. Bio-Informatic Analysis and Visualization
3. Results
3.1. Transcriptomic Profiling Reveals Novel Dysregulated Genes in PAD
3.2. Gene Ontology Analysis Identifies Key Pathways in PAD
3.3. Gene Networks Involved in PAD Pathogenesis Reveal Novel Interactions
3.4. Non-Coding RNA Analysis Identified Novel Non-Coding RNAs in PAD
3.5. Cell Profiling Predicts Key Cell Types Involved in PAD
3.6. Meta-Analysis Identifies Unique and Common Genes in PAD
4. Discussion
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Machiraju, P.; Srinivas, R.; Kannan, R.; George, R.; Heymans, S.; Mukhopadhyay, R.; Ghosh, A. Paired Transcriptomic Analyses of Atheromatous and Control Vessels Reveal Novel Autophagy and Immunoregulatory Genes in Peripheral Artery Disease. Cells 2024, 13, 1269. https://doi.org/10.3390/cells13151269
Machiraju P, Srinivas R, Kannan R, George R, Heymans S, Mukhopadhyay R, Ghosh A. Paired Transcriptomic Analyses of Atheromatous and Control Vessels Reveal Novel Autophagy and Immunoregulatory Genes in Peripheral Artery Disease. Cells. 2024; 13(15):1269. https://doi.org/10.3390/cells13151269
Chicago/Turabian StyleMachiraju, Praveen, Rajesh Srinivas, Ramaraj Kannan, Robbie George, Stephane Heymans, Rupak Mukhopadhyay, and Arkasubhra Ghosh. 2024. "Paired Transcriptomic Analyses of Atheromatous and Control Vessels Reveal Novel Autophagy and Immunoregulatory Genes in Peripheral Artery Disease" Cells 13, no. 15: 1269. https://doi.org/10.3390/cells13151269