Rousettus aegyptiacus Fruit Bats Do Not Support Productive Replication of Cedar Virus upon Experimental Challenge
Abstract
:1. Introduction
2. Materials and Methods
2.1. CedV Acquisition and Propagation
2.2. R. aegyptiacus Challenge Experiment
2.3. Total RNA Extraction and Detection of Viral RNA by Real-Time RTq-PCR
2.4. Monitoring of Body Core Temperature and Locomotor Activity Using Data Loggers
2.5. Serological Analysis
3. Results
3.1. Clinical Signs and Virological Analysis
3.2. Serology
3.3. Body Temperature and Locomotor Activity of Selected Individuals
4. Discussion
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Animal | Age Group | dpi | Sample | Ct-Value RT-qPCR |
---|---|---|---|---|
FH 3 | adult | 2 | Nasal lavage Anal swab Organ: nasal conchae | 36.97 35.11 39.2 |
FH 4 | pup of FH 3 | 2 | Organ: kidney | 37.6 |
FH 5 | adult | 2 | Organ: nasal conchae | 36.9 |
FH 6 | pup of FH 5 | 2 | Oral swab Nasal lavage | 38.72 39.13 |
Animal ID and Status | PP CedV G Protein | PP CedV N Protein |
---|---|---|
FH 1 (mock-inoculated juvenile) | 4.0 | 3.5 |
FH 2 (mock-inoculated juvenile) | 2.9 | 3.4 |
FH 3 (inoculated adult; necropsy 2 dpi) | 3.1 | 3.3 |
FH 4 (inoculated pup of FH 3; necropsy 2 dpi) | 3.6 | 3.7 |
FH 5 (inoculated adult; necropsy 2 dpi) | 4.6 | 4.1 |
FH 6 (inoculated pup of FH 5; necropsy 2 dpi) | 4.3 | 6.2 |
FH 7 (inoculated adult; necropsy 6 dpi) | 3.1 | 3.7 |
FH 8 (inoculated pup of FH 7; necropsy 6 dpi) | 3.3 | 3.6 |
FH 9 (inoculated adult; necropsy 14 dpi) | 4.2 | 3.5 |
FH 10 (inoculated pup of FH 9; necropsy 14 dpi) | 5.1 | 2.9 |
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Mohl, B.-P.; Diederich, S.; Fischer, K.; Balkema-Buschmann, A. Rousettus aegyptiacus Fruit Bats Do Not Support Productive Replication of Cedar Virus upon Experimental Challenge. Viruses 2024, 16, 1359. https://doi.org/10.3390/v16091359
Mohl B-P, Diederich S, Fischer K, Balkema-Buschmann A. Rousettus aegyptiacus Fruit Bats Do Not Support Productive Replication of Cedar Virus upon Experimental Challenge. Viruses. 2024; 16(9):1359. https://doi.org/10.3390/v16091359
Chicago/Turabian StyleMohl, Björn-Patrick, Sandra Diederich, Kerstin Fischer, and Anne Balkema-Buschmann. 2024. "Rousettus aegyptiacus Fruit Bats Do Not Support Productive Replication of Cedar Virus upon Experimental Challenge" Viruses 16, no. 9: 1359. https://doi.org/10.3390/v16091359