Mechanism of Takifugu bimaculatus Skin Peptides in Alleviating Hyperglycemia in Rats with Type 2 Diabetic Mellitus Based on Microbiome and Metabolome Analyses
Abstract
:1. Introduction
2. Results
2.1. Characterizing TBSH
2.2. Effects of TBP on Basic Indexes in Rats with T2DM
2.3. Effect of TBP on Blood Glucose-Related Gut Hormones and Blood Lipid Indexes in Rats with T2DM
2.4. Effect of TBP on the Viscera of T2DM Rats
2.5. Effect of High-Dose TBP on the Composition of Gut Microbiota
2.6. Effect of TBPH on Untargeted Metabolome of Gut Samples
2.7. Correlation Analysis among the Gut Microbiota, Metabolites, and Physiological Indexes
3. Discussion
4. Materials and Methods
4.1. Materials
4.2. Preparing TBSH
4.3. Distribution of TBSH Molecular Weight
4.4. DPP-IV Inhibitory Activity In Vitro
4.5. Animal Experiments
4.6. Oral Glucose Tolerance Test (OGTT)
4.7. Blood Glucose-Related Gut Hormones and Blood Lipids
4.8. Liver and Pancreas Histological Analysis
4.9. Gut Microbiota Analysis
4.10. Fecal Metabolome Analysis
4.11. Statistical Analysis
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Data Availability Statement
Conflicts of Interest
References
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Xu, M.; Chen, B.; Qiao, K.; Liu, S.; Su, Y.; Cai, S.; Liu, Z.; Li, L.; Li, Q. Mechanism of Takifugu bimaculatus Skin Peptides in Alleviating Hyperglycemia in Rats with Type 2 Diabetic Mellitus Based on Microbiome and Metabolome Analyses. Mar. Drugs 2024, 22, 377. https://doi.org/10.3390/md22080377
Xu M, Chen B, Qiao K, Liu S, Su Y, Cai S, Liu Z, Li L, Li Q. Mechanism of Takifugu bimaculatus Skin Peptides in Alleviating Hyperglycemia in Rats with Type 2 Diabetic Mellitus Based on Microbiome and Metabolome Analyses. Marine Drugs. 2024; 22(8):377. https://doi.org/10.3390/md22080377
Chicago/Turabian StyleXu, Min, Bei Chen, Kun Qiao, Shuji Liu, Yongchang Su, Shuilin Cai, Zhiyu Liu, Lijun Li, and Qingbiao Li. 2024. "Mechanism of Takifugu bimaculatus Skin Peptides in Alleviating Hyperglycemia in Rats with Type 2 Diabetic Mellitus Based on Microbiome and Metabolome Analyses" Marine Drugs 22, no. 8: 377. https://doi.org/10.3390/md22080377