Identification of Shaker Potassium Channel Family Members and Functional Characterization of SsKAT1.1 in Stenotaphrum secundatum Suggest That SsKAT1.1 Contributes to Cold Resistance
Abstract
:1. Introduction
2. Results
2.1. Gene Identification
2.2. Motif and Protein Domain Analysis
2.3. Protein Properties
2.4. Protein Secondary Structure and Three-Dimensional Structure Prediction
2.5. Analysis of the Phylogenetic Relationship of the Shaker K+ Channel
2.6. Relative Expression Levels of Shaker K+ Channel Family Members in Different Tissues
2.7. SsKAT1.1 Is Located to Plasma Membrane
2.8. SsKAT1.1 Has a Potassium Absorption Function
2.9. Expression of SsKAT1.1 in Yeast Enhances Cold Resistance
2.10. Expression of SsKAT1.1 in Yeast Enhances Salt Tolerance
3. Discussion
3.1. Stenotaphrum secundatum and Setaria italica Shaker K+ Channels Have a Close Phylogenetic Relationship
3.2. Shaker K+ Channel Members of Stenotaphrum secundatum Have More than One Corresponding Gene
3.3. Several Shaker K+ Channels in Stenotaphrum secundatum Have Not Been Identified
3.4. SsKAT1.1 Has a Potassium Absorption Function, and Its Potassium Absorption Function Depends on Higher Concentrations of K+
3.5. SsKAT1.1 Participates in Cellular Cold Resistance
3.6. SsKAT1.1 Participates in Cell Salt Tolerance
4. Materials and Methods
4.1. Plant Material Culture
4.2. Sequencing and Identification of Shaker K+ Channel Genes in Stenotaphrum secundatum
4.3. Protein Analysis, Structure Prediction, and Three-Dimensional Modelling
4.4. Motif and Protein Domain Analysis
4.5. Evolutionary Tree Construction
4.6. Construction of the SsKAT1.1-PYES2 Yeast Expression Vector and Yeast Transformation
4.7. qRT-PCR
4.8. Subcellular Location Analysis in Tobacco
4.9. Yeast Assays
5. Conclusions
Supplementary Materials
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
Abbreviations
References
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Gene Name | CDS Length | Number of Encoded Amino Acids |
---|---|---|
SsKAT1.1 | 2196 | 731 |
SsKAT1.2 | 2196 | 731 |
SsKAT2.1 | 1761 | 586 |
SsKAT2.2 | 1689 | 562 |
SsAKT1.1 | 2151 | 716 |
SsAKT1.2 | 1302 | 433 |
SsAKT2.1 | 2184 | 727 |
SsAKT2.2 | 2178 | 725 |
SsKOR1 | 2559 | 852 |
Proteins | pI | Molecular Weight (KD) | Instability Coefficient | GRAVY |
---|---|---|---|---|
SsKAT1.1 | 6.61 | 84.18 | 38.63 | −0.223 |
SsKAT1.2 | 6.93 | 84.20 | 39.09 | −0.256 |
SsKAT2.1 | 7.25 | 66.35 | 39.12 | −0.247 |
SsKAT2.2 | 8.06 | 63.98 | 39.61 | −0.287 |
SsAKT1.1 | 6.51 | 80.69 | 36.62 | −0.231 |
SsAKT2.1 | 6.57 | 81.12 | 37.5 | −0.09 |
SsAKT2.2 | 6.67 | 80.97 | 38.01 | −0.09 |
SsKOR1 | 6.00 | 96.56 | 44.25 | −0.123 |
Proteins | Alpha Helix (%) | Beta Turn (%) | Random Coil (%) | Extended Strand (%) |
---|---|---|---|---|
SsKAT1.1 | 48.43 | 3.15 | 36.11 | 12.31 |
SsKAT1.2 | 48.02 | 3.15 | 36.53 | 12.31 |
SsKAT2.1 | 49.49 | 2.39 | 35.49 | 12.63 |
SsKAT2.2 | 50 | 3.38 | 33.27 | 13.35 |
SsAKT1.1 | 47.49 | 7.82 | 37.43 | 7.26 |
SsAKT2.1 | 55.57 | 6.05 | 27.79 | 10.59 |
SsAKT2.2 | 52.83 | 5.66 | 29.52 | 12 |
SsKOR1 | 53.52 | 4.81 | 31.57 | 10.09 |
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Hao, D.-L.; Qu, J.; Wang, Z.-Y.; Sun, D.-J.; Yang, S.-N.; Liu, J.-X.; Zong, J.-Q.; Lu, H.-L. Identification of Shaker Potassium Channel Family Members and Functional Characterization of SsKAT1.1 in Stenotaphrum secundatum Suggest That SsKAT1.1 Contributes to Cold Resistance. Int. J. Mol. Sci. 2024, 25, 9480. https://doi.org/10.3390/ijms25179480
Hao D-L, Qu J, Wang Z-Y, Sun D-J, Yang S-N, Liu J-X, Zong J-Q, Lu H-L. Identification of Shaker Potassium Channel Family Members and Functional Characterization of SsKAT1.1 in Stenotaphrum secundatum Suggest That SsKAT1.1 Contributes to Cold Resistance. International Journal of Molecular Sciences. 2024; 25(17):9480. https://doi.org/10.3390/ijms25179480
Chicago/Turabian StyleHao, Dong-Li, Jia Qu, Zhi-Yong Wang, Dao-Jin Sun, Sheng-Nan Yang, Jian-Xiu Liu, Jun-Qin Zong, and Hai-Long Lu. 2024. "Identification of Shaker Potassium Channel Family Members and Functional Characterization of SsKAT1.1 in Stenotaphrum secundatum Suggest That SsKAT1.1 Contributes to Cold Resistance" International Journal of Molecular Sciences 25, no. 17: 9480. https://doi.org/10.3390/ijms25179480