Anti-Inflammatory Effects of miR-369-3p via PDE4B in Intestinal Inflammatory Response
Abstract
:1. Introduction
2. Results
2.1. In Silico Analysis of miR-369-3p Gene Targets
2.2. PDE4B Modulation by miR-369-3p
2.3. miR-369-3p Regulates cAMP Expression
2.4. miR-369-3p Affects the Downstream PDE4B Signaling Pathways
2.5. Effects of miR-369-3p on Anti-Inflammatory and Pro-Inflammatory Cytokine Release
2.6. PDE4B Expression in IBD Patients
3. Discussion
4. Materials and Methods
4.1. Cell Culture and miR-369-3p Mimic Transfection
4.2. RNA Extraction and Real-Time PCR
4.3. Protein Isolation and Immunoblotting Analysis
4.4. Measurement of Cytokine Production
4.5. Immunofluorescence
4.6. Immunohistochemistry in UC Samples
4.7. Bioinformatics and Statistical Analysis
5. Conclusions
6. Patents
Author Contributions
Funding
Institutional Review Board Statement
Informed Consent Statement
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Scalavino, V.; Piccinno, E.; Labarile, N.; Armentano, R.; Giannelli, G.; Serino, G. Anti-Inflammatory Effects of miR-369-3p via PDE4B in Intestinal Inflammatory Response. Int. J. Mol. Sci. 2024, 25, 8463. https://doi.org/10.3390/ijms25158463
Scalavino V, Piccinno E, Labarile N, Armentano R, Giannelli G, Serino G. Anti-Inflammatory Effects of miR-369-3p via PDE4B in Intestinal Inflammatory Response. International Journal of Molecular Sciences. 2024; 25(15):8463. https://doi.org/10.3390/ijms25158463
Chicago/Turabian StyleScalavino, Viviana, Emanuele Piccinno, Nicoletta Labarile, Raffaele Armentano, Gianluigi Giannelli, and Grazia Serino. 2024. "Anti-Inflammatory Effects of miR-369-3p via PDE4B in Intestinal Inflammatory Response" International Journal of Molecular Sciences 25, no. 15: 8463. https://doi.org/10.3390/ijms25158463