Glycan Node Analysis Detects Varying Glycosaminoglycan Levels in Melanoma-Derived Extracellular Vesicles
Abstract
:1. Introduction
2. Results
3. Discussion
4. Conclusions
5. Materials and Methods
5.1. Cell Culture
5.2. EV Isolation via Tangential Flow Filtration (TFF)
5.3. Nanoparticle Tracking Analysis (NTA)
5.4. Cryo-TEM
5.5. Western Blot
5.6. GNA
5.7. Hyaluronan Quantification and Enzymatic Stripping
5.8. Statistical Analysis
Author Contributions
Funding
Data Availability Statement
Acknowledgments
Conflicts of Interest
References
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Pendiuk Goncalves, J.; Walker, S.A.; Aguilar Díaz de león, J.S.; Yang, Y.; Davidovich, I.; Busatto, S.; Sarkaria, J.; Talmon, Y.; Borges, C.R.; Wolfram, J. Glycan Node Analysis Detects Varying Glycosaminoglycan Levels in Melanoma-Derived Extracellular Vesicles. Int. J. Mol. Sci. 2023, 24, 8506. https://doi.org/10.3390/ijms24108506
Pendiuk Goncalves J, Walker SA, Aguilar Díaz de león JS, Yang Y, Davidovich I, Busatto S, Sarkaria J, Talmon Y, Borges CR, Wolfram J. Glycan Node Analysis Detects Varying Glycosaminoglycan Levels in Melanoma-Derived Extracellular Vesicles. International Journal of Molecular Sciences. 2023; 24(10):8506. https://doi.org/10.3390/ijms24108506
Chicago/Turabian StylePendiuk Goncalves, Jenifer, Sierra A. Walker, Jesús S. Aguilar Díaz de león, Yubo Yang, Irina Davidovich, Sara Busatto, Jann Sarkaria, Yeshayahu Talmon, Chad R. Borges, and Joy Wolfram. 2023. "Glycan Node Analysis Detects Varying Glycosaminoglycan Levels in Melanoma-Derived Extracellular Vesicles" International Journal of Molecular Sciences 24, no. 10: 8506. https://doi.org/10.3390/ijms24108506