TmSpz4 Plays an Important Role in Regulating the Production of Antimicrobial Peptides in Response to Escherichia coli and Candida albicans Infections
Abstract
:1. Introduction
2. Results
2.1. Sequence Identification and Phylogenetic Analysis Of TmSpz4
2.2. Developmental and Tissue-Specific Expression Patterns of TmSpz4
2.3. Temporal Induction Pattern of TmSpz4
2.4. Effect of TmSpz4 RNAi on T. Molitor Survivability
2.5. Effects of TmSpz4 Gene Silencing on the Expression of AMPs
3. Discussion
4. Materials and Methods
4.1. Insect Culture
4.2. Preparation of Microorganisms
4.3. Identification and Cloning of Full-Length cDNA Sequence of TmSpz4
4.4. Domain and Phylogenetic Analysis
4.5. TmSpz4 Expression and Temporal Induction Pattern Analysis
4.6. Effect of TmSpz4 Gene Silencing in Response to Microorganisms
4.7. Effect of TmSpz4 RNAi on AMP Expression against Microbial Challenge
4.8. Effects of dsTmSpz4 on the Expression Patterns of NF-κB Genes
4.9. Data Analysis
Supplementary Materials
Author Contributions
Funding
Conflicts of Interest
References
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Primer Name | Sequence (5′-3′) |
---|---|
TmSpz4-qPCR-Fw | GGCGATGCTCTTCCAGGAC |
TmSpz4-qPCR-Rv | CGCGTTCACTCCTTTCATTTGG |
TmSpz4-T7-Fw | TAATACGACTCACTATAGGGTCCAGATGTACTGTCGCGATG |
TmSpz4-T7-Rv | TAATACGACTCACTATAGGGTTTCCTTCTGTACCAGTCGGG |
TmSpz4-cloning-Fw | ACCGACACAACCACCAAAAG |
TmSpz4-cloning-Rv | ATCCGTGATCTCCGGAAAAA |
TmSpz4-cloning-FullORF-Fw | GACGGTGCCCACGAACTAT |
TmSpz4-cloning-FullORF-Rv | AAGAGCACGAGCCTTGACAT |
TmL27a_qPCR_Fw | TCATCCTGAAGGCAAAGCTCCAGT |
TmL27a_qPCR_Rv | AGGTTGGTTAGGCAGGCACCTTTA |
dsEGFP_Fw | TAATACGACTCACTATAGGGTCGTAAACGGCCACAAGTTC |
dsEGFP_Rv | TAATACGACTCACTATAGGGT TGCTCAGGTAGTGTTGTCG |
TmTenecin-1_Fw | CAGCTGAAGAAATCGAACAAGG |
TmTenecin-1_Rv | CAGACCCTCTTTCCGTTACAGT |
TmTenecin-2_Fw | CAGCAAAACGGAGGATGGTC |
TmTenecin-2_Rv | CGTTGAAATCGTGATCTTGTCC |
TmTenecin-3_Fw | GATTTGCTTGATTCTGGTGGTC |
TmTenecin-3_Rv | CTGATGGCCTCCTAAATGTCC |
TmTenecin-4_Fw | GGACATTGAAGATCCAGGAAAG |
TmTenecin-4_Rv | CGGTGTTCCTTATGTAGAGCTG |
TmDefensin-1_Fw | AAATCGAACAAGGCCAACAC |
TmDefencin-1_Rv | GCAAATGCAGACCCTCTTTC |
TmDefencin-2_Fw | GGGATGCCTCATGAAGATGTAG |
TmDefencin-2_Rv | CCAATGCAAACACATTCGTC |
TmColoptericin-1_Fw | GGACAGAATGGTGGATGGTC |
TmColoptericin-1_Rv | CTCCAACATTCCAGGTAGGC |
TmColoptericin-2_Fw | GGACGGTTCTGATCTTCTTGAT |
TmColoptericin-2_Rv | CAGCTGTTTGTTTGTTCTCGTC |
TmAttacin-1a_Fw | GAAACGAAATGGAAGGTGGA |
TmAttacin-1a_Rv | TGCTTCGGCAGACAATACAG |
TmAttacin-1b_Fw | GAGCTGTGAATGCAGGACAA |
TmAttacin-1b_Rv | CCCTCTGATGAAACCTCCAA |
TmAttacin-2_Fw | AACTGGGATATTCGCACGTC |
TmAttacin-2_Rv | CCCTCCGAAATGTCTGTTGT |
TmCecropin-2_Fw | TACTAGCAGCGCCAAAACCT |
TmCecropin-2_Rv | CTGGAACATTAGGCGGAGAA |
TmThaumatin-likeprotein-1_Fw | CTCAAAGGACACGCAGGACT |
TmThaumatin-like protein-1_Rv | ACTTTGAGCTTCTCGGGACA |
TmThaumatin-like protein-2_Fw | CCGTCTGGCTAGGAGTTCTG |
TmThaumatin-like protein-2_Rv | ACTCCTCCAGCTCCGTTACA |
TmDorsal-X1_qPCR_Fw | AGCGTTGAGGTTTCGGTATG |
TmDorsal-X1_qPCR_Rv | TCTTTGGTGACGCAAGACAC |
TmDorsal-X2_qPCR_Fw | ACACCCCCGAAATCACAAAC |
TmDorsal-X2_qPCR_Rv | TTTCAGAGCGCCAGGTTTTG |
TmRelish_qPCR_Fw | AGCGTCAAGTTGGAGCAGAT |
TmRelish_qPCR_Rv | GTCCGGACCTCAAGTGT |
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Edosa, T.T.; Jo, Y.H.; Keshavarz, M.; Bae, Y.M.; Kim, D.H.; Lee, Y.S.; Han, Y.S. TmSpz4 Plays an Important Role in Regulating the Production of Antimicrobial Peptides in Response to Escherichia coli and Candida albicans Infections. Int. J. Mol. Sci. 2020, 21, 1878. https://doi.org/10.3390/ijms21051878
Edosa TT, Jo YH, Keshavarz M, Bae YM, Kim DH, Lee YS, Han YS. TmSpz4 Plays an Important Role in Regulating the Production of Antimicrobial Peptides in Response to Escherichia coli and Candida albicans Infections. International Journal of Molecular Sciences. 2020; 21(5):1878. https://doi.org/10.3390/ijms21051878
Chicago/Turabian StyleEdosa, Tariku Tesfaye, Yong Hun Jo, Maryam Keshavarz, Young Min Bae, Dong Hyun Kim, Yong Seok Lee, and Yeon Soo Han. 2020. "TmSpz4 Plays an Important Role in Regulating the Production of Antimicrobial Peptides in Response to Escherichia coli and Candida albicans Infections" International Journal of Molecular Sciences 21, no. 5: 1878. https://doi.org/10.3390/ijms21051878