Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training
Abstract
:1. Introduction
2. Results
2.1. Subject Characteristics
2.2. Muscle Lipids and Glycogen
2.3. Protein Expression
3. Discussion
Limitations
4. Methods and Materials
4.1. Subjects
4.2. Study Design
4.3. High-Intensity Interval Training (HIIT) Protocol
4.4. Maximal Oxygen Uptake
4.5. Blood Analyses
4.6. Muscle Biopsies
4.7. Lipid Analyses
4.8. Intramyocellular Triglyceride and Glycogen
4.9. Citrate Synthase and β-Hydroxyacyl-CoA Dehydrogenase
4.10. Western Blot
4.11. Statistical Analyses
5. Conclusions
Author Contributions
Funding
Acknowledgments
Conflicts of Interest
References
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Young (n = 14) Pre Post | Older (n = 22) Pre Post | Main Effect (p-value) Age Time | Interaction (p-value) Group x Time | ||||
---|---|---|---|---|---|---|---|
Gender (F/M) | 5/9 | 11/11 | |||||
Age (yrs) | 32 ± 2 | 63 ± 1 | |||||
Height (m) | 1.78 ± 0.02 | 1.70 ± 0.02 | 0.014 | NS | NS | ||
Weight (kg) | 110 ± 4 | 110 ± 4 | 88.7 ± 2.6 | 88.4 ± 2.6 | <0.001 | NS | NS |
BMI (kg·m−2) | 34.8 ± 1.0 | 34.6 ± 1.0 | 30.7 ± 0.7 | 30.6 ± 0.7 | 0.003 | NS | NS |
LBM (kg) | 63.8 ± 2.1 | 64.7 ± 2.3 | 51.5 ± 2.1 | 51.8 ± 2.1 | <0.001 | <0.001 | 0.099 |
Fat mass (kg) | 40.3 ± 3.1 | 39.3 ± 3.3 | 34.0 ± 1.6 | 33.3 ± 1.7 | NS | 0.016 | NS |
Fat % | 39.2 ± 2.1 | 38.2 ± 2.3 | 39.8 ± 1.6 | 39.1 ± 1.6 | NS | <0.001 | NS |
Visceral fat (kg) | 1.67 ± 0.25 | 1.56 ± 0.24 | 1.90 ± 0.16 | 1.81 ± 0.16 | NS | 0.024 | NS |
HbA1c (%) | 5.3 ± 0.1 | 5.3 ± 0.1 | 5.7 ± 0.1 | 5.6 ± 0.1 | 0.002 | NS | NS |
HOMA-IR (AU) | 2.14 ± 0.24 | 2.31 ± 0.38 | 1.88 ± 0.23 | 1.99 ± 0.30 | NS | NS | NS |
Glucose, fasting (mmol·L−1) | 4.5 ± 0.1 | 4.5 ± 0.1 | 6.1 ± 0.2 | 6.0 ± 0.2 | <0.001 | NS | NS |
Insulin, fasting (pmol L−1) | 69.7 ± 9,5 | 67.2 ± 8.9 | 40.9 ± 4.8 | 42.6 ± 5.8 | 0.008 | NS | NS |
IMTG (mmol·kg−1 dw) | 126 ± 27 | 118 ± 21 | 156 ± 23 | 119 ± 12 | NS | NS | NS |
Glycogen (nmol·kg−1 dw) | 236 ± 30 | 474 ± 46 | 323 ± 24 | 483 ± 23 | NS | <0.001 | NS |
HAD (µmol·g−1·min−1) | 116 ± 7 | 130 ± 7 | 112 ± 10 | 141 ± 5 | NS | <0.001 | NS |
CS (µmol·g−1·min−1) | 132 ± 7 | 165 ± 8 | 122 ± 10 | 169 ± 10 | NS | <0.001 | NS |
VO2max (mL·min−1) | 3068 ± 131 | 3186 ± 118 | 2234 ± 106 | 2361 ± 134 | <0.001 | 0.021 | NS |
VO2max (mL·min−1·kg−1) | 28.3 ± 1.2 | 29.7 ± 1.5 | 25.2 ± 1.0 | 26.7 ± 1.1 | NS | 0.007 | NS |
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Søgaard, D.; Baranowski, M.; Larsen, S.; Taulo Lund, M.; Munk Scheuer, C.; Vestergaard Abildskov, C.; Greve Dideriksen, S.; Dela, F.; Wulff Helge, J. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. Int. J. Mol. Sci. 2019, 20, 1240. https://doi.org/10.3390/ijms20051240
Søgaard D, Baranowski M, Larsen S, Taulo Lund M, Munk Scheuer C, Vestergaard Abildskov C, Greve Dideriksen S, Dela F, Wulff Helge J. Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training. International Journal of Molecular Sciences. 2019; 20(5):1240. https://doi.org/10.3390/ijms20051240
Chicago/Turabian StyleSøgaard, Ditte, Marcin Baranowski, Steen Larsen, Michael Taulo Lund, Cathrine Munk Scheuer, Carina Vestergaard Abildskov, Sofie Greve Dideriksen, Flemming Dela, and Jørn Wulff Helge. 2019. "Muscle-Saturated Bioactive Lipids Are Increased with Aging and Influenced by High-Intensity Interval Training" International Journal of Molecular Sciences 20, no. 5: 1240. https://doi.org/10.3390/ijms20051240