Caffeic Acid Phenethyl Ester Inhibits UV-Induced MMP-1 Expression by Targeting Histone Acetyltransferases in Human Skin
Abstract
:1. Introduction
2. Results
2.1. CAPE Reduces UV-Induced MMP-1 Expression in Human Dermal Fibroblasts
2.2. CAPE Suppresses UV-Induced MMP-1 Expression in Human Skin Tissues
2.3. CAPE Inhibits UV-Stimulated Acetylation of Total Lysine and Histone H3 Lysine 9 in Both HDF Cells and Human Skin Tissues
2.4. CAPE Acts as an HAT Inhibitor
3. Discussion
4. Materials and Methods
4.1. Reagents
4.2. Cell Culture and UV Irradiation
4.3. Excised Human Skin and UV Irradiation
4.4. Cell Viability Assay
4.5. Enzyme-Linked Immunosorbent Assay (ELISA)
4.6. Real-Time PCR (qPCR)
4.7. Immunoblot Analysis
4.8. HAT Assay
4.9. Statistical Analysis
5. Conclusions
Author Contributions
Funding
Conflicts of Interest
Abbreviations
CAPE | Caffeic acid phenethyl ester |
UV | Ultraviolet |
MMP-1 | Matrix metalloproteinase |
HDF | Human dermal fibroblasts |
HAT | Histone acetyltransferase |
CBP | CREP-binding protein |
PCAF | p300/CBP-associated factor |
HDAC | Histone deacetylase |
MAPK | Mitogen-activated protein kinase |
ERK | Extracellular-signal-regulated kinase |
JNK | c-Jun N-terminal kinase |
AA | Anacardic acid |
COX-2 | Cyclooxygenase-2 |
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Shin, E.J.; Jo, S.; Choi, H.-k.; Choi, S.; Byun, S.; Lim, T.-G. Caffeic Acid Phenethyl Ester Inhibits UV-Induced MMP-1 Expression by Targeting Histone Acetyltransferases in Human Skin. Int. J. Mol. Sci. 2019, 20, 3055. https://doi.org/10.3390/ijms20123055
Shin EJ, Jo S, Choi H-k, Choi S, Byun S, Lim T-G. Caffeic Acid Phenethyl Ester Inhibits UV-Induced MMP-1 Expression by Targeting Histone Acetyltransferases in Human Skin. International Journal of Molecular Sciences. 2019; 20(12):3055. https://doi.org/10.3390/ijms20123055
Chicago/Turabian StyleShin, Eun Ju, Seongin Jo, Hyo-kyoung Choi, Sungbin Choi, Sanguine Byun, and Tae-Gyu Lim. 2019. "Caffeic Acid Phenethyl Ester Inhibits UV-Induced MMP-1 Expression by Targeting Histone Acetyltransferases in Human Skin" International Journal of Molecular Sciences 20, no. 12: 3055. https://doi.org/10.3390/ijms20123055