Isolation of Sulfur Reducing and Oxidizing Bacteria Found in Contaminated Drywall
Abstract
:1. Introduction
2. Results and Discussion
3. Experimental Section
3.1. The Hypothesis in This Study Tested the Following:
3.1.1. Null Hypothesis
3.1.2. Alternative Hypothesis
3.2. Samples
3.3. Extraction of Samples
3.4. Target Design
3.5. Assay Design
3.6. Assay Optimization
3.6.1. Rehydration of Lyophilized Stocks
3.6.2. Standard Curves
3.6.3. Testing of Spiked Drywall
3.6.4. Testing of Drywall Unknowns
4. Conclusions
References and Notes
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Thiobacillus (Actinobacillus) caldus | Designed with strains N39-30-02, P5-10, MTH-04, DSM8584, DX-2, J-1, D-2, YN06, S-1, T-1, S-2, D-1, and ATCC51756. |
Sulfobacillus thermosulfidooxidans | Designed with strains HR-K17-45, DK-J16-45, DK-E8-45, N19-45-01, N19-50-01, G-2, YN22, and ATCC 51911 (S. disulfidooxidans). |
Leptospirillum ferrooxidans | Designed with strains JCM17, C2-6, C21, C2-3, C2-7, C2-2, C2-4, and ATCC 53993 D. |
Actinobacillus thioxidans | Designed with strains ATCC 19377, and ATCC 55020. |
Thiobacillus (Actinobacillus) ferrooxidans | Designed with strains ATCC2327OT, ATCC33020, SS6, CC1, BRGM1, LMT1, LMT4, DSM9465, SS4, 84, ATCC19859, TF-49, B9, B20, B5, ML17-48A, and ATCC23270D. |
Organism | Standard Curves Drawn | Spiked Drywall Showing No Inhibition of Curves Number spiked/Number Positive | Drywall Unknowns Positive/Total Tested (Percent Positive] |
---|---|---|---|
Thiobacillus (Actinobacillus) caldus | Yes | 6/6 | 1 (weak)/25 (4.0%) |
Sulfobacillus thermosulfidoxidans | Yes | 6/6 | 0/25 (0%) |
Leptospirillum ferrooxidans | Yes | 6/6 | 0/25 (0%) |
Actinobacillus thioxidans | Yes | 6/6 | 0/25 (0%) |
Thiobacillus (Actinobacillus) ferrooxidans | Yes | 6/6 | 25/25(100%) |
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Hooper, D.G.; Shane, J.; Straus, D.C.; Kilburn, K.H.; Bolton, V.; Sutton, J.S.; Guilford, F.T. Isolation of Sulfur Reducing and Oxidizing Bacteria Found in Contaminated Drywall. Int. J. Mol. Sci. 2010, 11, 647-655. https://doi.org/10.3390/ijms11020647
Hooper DG, Shane J, Straus DC, Kilburn KH, Bolton V, Sutton JS, Guilford FT. Isolation of Sulfur Reducing and Oxidizing Bacteria Found in Contaminated Drywall. International Journal of Molecular Sciences. 2010; 11(2):647-655. https://doi.org/10.3390/ijms11020647
Chicago/Turabian StyleHooper, Dennis G., John Shane, David C. Straus, Kaye H. Kilburn, Vincent Bolton, John S. Sutton, and Frederick T. Guilford. 2010. "Isolation of Sulfur Reducing and Oxidizing Bacteria Found in Contaminated Drywall" International Journal of Molecular Sciences 11, no. 2: 647-655. https://doi.org/10.3390/ijms11020647