Proteomika
Proteomika je izučavanje proteina velikih razmera sa fokusom na njihovu strukturu i funkcije.[1][2] Proteini su vitalni delovi živih organizama. Oni su glavne komponente fizioloških metaboličkih puteva u ćelijama. Termin proteomika je formiran 1997[3] po analogiji sa genomikom, studijom genoma. Reč proteom je mešavina reči protein i genom, koju je formirao Mark Vilkins 1994.[4][5]
Proteom je celokupan set proteina[4] koji jedan organizam proizvodi ili modifikuje. Njegov sadržaj varira s vremenom i u zavisnosti od raznih uslova, stresova, kojima su ćelija ili ogranizam izloženi. Proteomika je interdisciplinarni domen koji je znatnim delom formiran na bazi istraživanja i razvoja Projekta ljudskog genoma. Ona obuhvata naučna istraživanja i ispitivanja proteoma sa sveobuhvatnog stanovišta intracelularne proteinske kompozicije, strukture, i njegovih jedinstvenih parterna dejstva. Proteomioka je važna komponenta funkcionalne genomike.
Mada se termin proteomika generalno odnosi na eksperimentalnu analizu proteina velikih razmera, on se isto tako koristi za proteinsko prečišćavanje i masenu spektrometriju.
Reference
[уреди | уреди извор]- ^ Anderson NL, Anderson NG (1998). „Proteome and proteomics: new technologies, new concepts, and new words”. Electrophoresis. 19 (11): 1853—61. PMID 9740045. doi:10.1002/elps.1150191103.
- ^ Blackstock WP, Weir MP (1999). „Proteomics: quantitative and physical mapping of cellular proteins”. Trends Biotechnol. 17 (3): 121—7. PMID 10189717. doi:10.1016/S0167-7799(98)01245-1.
- ^ P. James (1997). „Protein identification in the post-genome era: the rapid rise of proteomics.”. Quarterly reviews of biophysics. 30 (4): 279—331. PMID 9634650. doi:10.1017/S0033583597003399.
- ^ а б Marc R. Wilkins, Christian Pasquali, Ron D. Appel, Keli Ou, Olivier Golaz, Jean-Charles Sanchez, Jun X. Yan, Andrew. A. Gooley, Graham Hughes, Ian Humphery-Smith, Keith L. Williams & Denis F. Hochstrasser (1996). „From Proteins to Proteomes: Large Scale Protein Identification by Two-Dimensional Electrophoresis and Arnino Acid Analysis”. Nature Biotechnology. 14 (1): 61—65. PMID 9636313. doi:10.1038/nbt0196-61.
- ^ „UNSW Staff Bio: Professor Marc Wilkins”. Архивирано из оригинала 12. 02. 2009. г. Приступљено 05. 11. 2013.
Literatura
[уреди | уреди извор]- Belhajjame, K. et al. Proteome Data Integration: Characteristics and Challenges. Proceedings of the UK e-Science All Hands Meeting, ISBN 1-904425-53-4, September 2005, Nottingham, UK.
- Twyman RM (2004). Principles Of Proteomics (Advanced Text Series). Oxford, UK: BIOS Scientific Publishers. ISBN 1-85996-273-4. (covers almost all branches of proteomics)
- Naven T, Westermeier R (2002). Proteomics in Practice: A Laboratory Manual of Proteome Analysis. Weinheim: Wiley-VCH. ISBN 3-527-30354-5. (focused on 2D-gels, good on detail)
- Liebler DC (2002). Introduction to proteomics: tools for the new biology. Totowa, NJ: Humana Press. ISBN 0-89603-992-7. ISBN 0-585-41879-9 (electronic, on Netlibrary?), ISBN 0-89603-991-9 hbk
- Wilkins MR, Williams KL, Appel RD, Hochstrasser DF (1997). Proteome Research: New Frontiers in Functional Genomics (Principles and Practice). Berlin: Springer. ISBN 3-540-62753-7.
- Arora PS, Yamagiwa H, Srivastava A, Bolander ME, Sarkar G (2005). „Comparative evaluation of two two-dimensional gel electrophoresis image analysis software applications using synovial fluids from patients with joint disease”. J Orthop Sci. 10 (2): 160—6. PMID 15815863. doi:10.1007/s00776-004-0878-0.[мртва веза]
- Rediscovering Biology Online Textbook. Unit 2 Proteins and Proteomics. 1997–2006.
- Weaver RF (2005). Molecular biology (3rd изд.). New York: McGraw-Hill. стр. 840—9. ISBN 0-07-284611-9.
- Reece J, Campbell N (2002). Biology (6th изд.). San Francisco: Benjamin Cummings. стр. 392-3. ISBN 0-8053-6624-5.
- Hye A; Lynham, S; M, Thambisetty; et al. (2006). „Proteome-based plasma biomarkers for Alzheimer's disease”. Brain. 129 (Pt 11): 3042—50. PMID 17071923. doi:10.1093/brain/awl279.
- Perroud B; Lee, J; N, Valkova; et al. (2006). „Pathway analysis of kidney cancer using proteomics and metabolic profiling”. Mol Cancer. 5: 64. PMC 1665458
. PMID 17123452. doi:10.1186/1476-4598-5-64. - Yohannes E, Chang J, Christ GJ, Davies KP, Chance MR (2008). „Proteomics analysis identifies molecular targets related to diabetes mellitus-associated bladder dysfunction”. Mol. Cell Proteomics. 7 (7): 1270—85. PMC 2493381 . PMID 18337374. doi:10.1074/mcp.M700563-MCP200..
- Macaulay IC, Carr P, Gusnanto A, Ouwehand WH, Fitzgerald D, Watkins NA (2005). „Platelet genomics and proteomics in human health and disease”. J Clin Invest. 115 (12): 3370—7. PMC 1297260 . PMID 16322782. doi:10.1172/JCI26885.
- Rogers MA; Clarke, P; J, Noble; et al. (15. 10. 2003). „Proteomic profiling of urinary proteins in renal cancer by surface enhanced laser desorption ionization and neural-network analysis: identification of key issues affecting potential clinical utility”. Cancer Res. 63 (20): 6971—83. PMID 14583499.
- Vasan RS (2006). „Biomarkers of cardiovascular disease: molecular basis and practical considerations”. Circulation. 113 (19): 2335—62. PMID 16702488. doi:10.1161/CIRCULATIONAHA.104.482570.
- "Myocardial Infarction". (Retrieved 29 November 2006)
- Introduction to Antibodies – Enzyme-Linked Immunosorbent Assay (ELISA). (Retrieved 29 November 2006)
- Decramer S; Wittke, S; H, Mischak; et al. (2006). „Predicting the clinical outcome of congenital unilateral ureteropelvic junction obstruction in newborn by urinary proteome analysis”. Nat Med. 12 (4): 398—400. PMID 16550189. doi:10.1038/nm1384.
- Mayer U (2008). „Protein Information Crawler (PIC): extensive spidering of multiple protein information resources for large protein sets”. Proteomics. 8 (1): 42—4. PMID 18095364. doi:10.1002/pmic.200700865.
- Jörg von Hagen, VCH-Wiley 2008 Proteomics Sample Preparation. ISBN 978-3-527-31796-7.
Vidi još
[уреди | уреди извор]Spoljašnje veze
[уреди | уреди извор]
- Proteomics на сајту Curlie (језик: енглески)
