Table of Contents

REVIEWS

Select this article
The roles and regulation of MDM2 and MDMX: it is not just about p53
- Alyssa M. Klein,
- Rafaela Muniz de Queiroz,
- Divya Venkatesh,
- and Carol Prives
Genes Dev. May 1, 2021 35: 575-601; Published in Advance April 22, 2021, doi:10.1101/gad.347872.120
In this review, Klein et al. discuss the p53-independent roles of MDM2 and MDMX. First, they review the structural and functional features of MDM2 and MDMX proteins separately and together that could be relevant to their p53-independent activities. Following this, they summarize how these two proteins are regulated and how they can function in cells that lack p53.
Select this article
Interfaces between cellular responses to DNA damage and cancer immunotherapy
- Domenic Pilger,
- Leonard W. Seymour,
- and Stephen P. Jackson
Genes Dev. May 1, 2021 35: 602-618; Published in Advance April 22, 2021, doi:10.1101/gad.348314.121
OPEN ACCESS ARTICLE
In this review, Pilger et al. summarize the current understanding of the molecular mechanisms underlying DNA damage-triggered immune responses, including cytosolic DNA sensing via the cGAS/STING pathway. They also highlight the implications of DDR components for therapeutic outcomes of immune checkpoint inhibitors or their use as biomarkers.
Select this article
Establishing and maintaining fertility: the importance of cell cycle arrest
- Emily R. Frost,
- Güneş Taylor,
- Mark A. Baker,
- Robin Lovell-Badge,
- and Jessie M. Sutherland
Genes Dev. May 1, 2021 35: 619-634; Published in Advance April 22, 2021, doi:10.1101/gad.348151.120
OPEN ACCESS ARTICLE
In this review, Frost et al. summarize the current knowledge on the Cip/Kip family of cyclin-dependent kinase inhibitors in mouse gonad development and highlight new roles for cell cycle inhibitors in controlling and maintaining female fertility.
Select this article
Hepatic transcriptional responses to fasting and feeding
- Lara Bideyan,
- Rohith Nagari,
- and Peter Tontonoz
Genes Dev. May 1, 2021 35: 635-657; Published in Advance April 22, 2021, doi:10.1101/gad.348340.121
In this review, Bideyan et al. summarize the advances in our understanding of the gene regulatory networks that contribute to hepatic responses to fasting and feeding. They also discuss how dietary modifications modulate hepatic gene expression programs.
Select this article
Protein phosphatases in the RNAPII transcription cycle: erasers, sculptors, gatekeepers, and potential drug targets
- Giacomo Cossa,
- Pabitra K. Parua,
- Martin Eilers,
- and Robert P. Fisher
Genes Dev. May 1, 2021 35: 658-676; Published in Advance April 22, 2021, doi:10.1101/gad.348315.121
In this review, Cossa et al. discuss the current knowledge and outstanding questions about phosphatases in the context of the RNAPII transcription cycle.
Select this article
Building a circuit through correlated spontaneous neuronal activity in the developing vertebrate and invertebrate visual systems
- Ben Jiwon Choi,
- Yu-Chieh David Chen,
- and Claude Desplan
Genes Dev. May 1, 2021 35: 677-691; Published in Advance April 22, 2021, doi:10.1101/gad.348241.121
In this review, Choi et al. compare the spontaneous activity of the developing visual system between mammals and Drosophila and suggest that Drosophila is an emerging model for mechanistic and functional studies of correlated spontaneous activity.

RESEARCH COMMUNICATION

Select this article
Meikin synergizes with shugoshin to protect cohesin Rec8 during meiosis I
- Wei Ma,
- Jingwen Zhou,
- Jian Chen,
- Antony M. Carr,
- and Yoshinori Watanabe
Genes Dev. May 1, 2021 35: 692-697; Published in Advance April 22, 2021, doi:10.1101/gad.348052.120
OPEN ACCESS ARTICLE
In this study, Ma et al. set out to study the mechanism of centromeric cohesion protection by Moa1/meikin during meiosis I. Using genetic and fluorescence microscopy approaches in fission yeast, the authors show that Moa1-Plo1 phosphorylates Rec8, which is required for Sgo1-PP2A to protect centromeric cohesion by antagonizing CK1-mediated phosphorylation of the cohesion subunit Rec8.

RESEARCH PAPERS

Select this article
Essential histone chaperones collaborate to regulate transcription and chromatin integrity
- Olga Viktorovskaya,
- James Chuang,
- Dhawal Jain,
- Natalia I. Reim,
- Francheska López-Rivera,
- Magdalena Murawska,
- Dan Spatt,
- L. Stirling Churchman,
- Peter J. Park,
- and Fred Winston
Genes Dev. May 1, 2021 35: 698-712; Published in Advance April 22, 2021, doi:10.1101/gad.348431.121
Here, Viktorovskaya et al. sought to understand how the functions of three essential histone chaperones are coordinated during gene transcription. Using genetic and biochemical approaches, this work sheds light on previously unknown connections between FACT activity and the functions of Spt6 and Spn1.
Select this article
MED1 is a lipogenesis coactivator required for postnatal adipose expansion
- Younghoon Jang,
- Young-Kwon Park,
- Ji-Eun Lee,
- Danyang Wan,
- Nhien Tran,
- Oksana Gavrilova,
- and Kai Ge
Genes Dev. May 1, 2021 35: 713-728; Published in Advance April 22, 2021, doi:10.1101/gad.347583.120
OPEN ACCESS ARTICLE
In this study, Jang et al. investigated the role of MED1 in adipose development and expansion in vivo, and they show that MED1 is not generally required for transcription during adipogenesisin culture and that MED1 is dispensable for adipose development in mice. Instead, MED1 is required for postnatal adipose expansion and the induction of fatty acid and triglyceride synthesis genes after pups switch diet from high-fat maternal milk to carbohydrate-based chow. Their findings identify a cell- and gene-specific regulatory role of MED1 as a lipogenesis coactivator required for postnatal adipose expansion.
Select this article
Critical roles of transcriptional coactivator MED1 in the formation and function of mouse adipose tissues
- Keiichi Ito,
- Marc Schneeberger,
- Alan Gerber,
- Miki Jishage,
- Francois Marchildon,
- Aarthi V. Maganti,
- Paul Cohen,
- Jeffrey M. Friedman,
- and Robert G. Roeder
Genes Dev. May 1, 2021 35: 729-748; Published in Advance April 22, 2021, doi:10.1101/gad.346791.120
In this study, Ito et al. sought to understand the precise roles of MED1, and its various domains, at various stages of adipogenesis and in adipose tissue. Using multiple genetic approaches to assess requirements for MED1 in adipocyte formation and function in mice, they show that MED1 is indeed essential for the differentiation and/or function of both brown and white adipocytes, as its absence in these cells leads to, respectively, defective brown fat function and lipodystrophy.
Select this article
BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome
- Nadezda A. Fursova,
- Anne H. Turberfield,
- Neil P. Blackledge,
- Emma L. Findlater,
- Anna Lastuvkova,
- Miles K. Huseyin,
- Paula Dobrinić,
- and Robert J. Klose
Genes Dev. May 1, 2021 35: 749-770; Published in Advance April 22, 2021, doi:10.1101/gad.347005.120
OPEN ACCESS ARTICLE
Here, Fursova et al. investigated the role of BAP1, an H2AK119ub1 deubiquitylating enzyme, in regulating gene expression in mouse ESCs. Using inducible genetic perturbations and quantitative genomics, they show that BAP1 plays a widespread role in promoting gene expression and that this relies on BAP1 counteracting pervasive H2AK119ub1. In the absence of BAP1, accumulation of H2AK119ub1 throughout the genome leads to widespread reductions in transcription.

RESOURCE/METHODOLOGY

Select this article
AGO HITS-CLIP reveals distinct miRNA regulation of white and brown adipose tissue identity
- Sean O'Connor,
- Elisabeth A. Murphy,
- Sarah K. Szwed,
- Matt Kanke,
- François Marchildon,
- Praveen Sethupathy,
- Robert B. Darnell,
- and Paul Cohen
Genes Dev. May 1, 2021 35: 771-781; Published in Advance April 8, 2021, doi:10.1101/gad.345447.120
In this Resource/Methodology, O'Connor et al. performed high-throughput sequencing of RNA isolated by cross-linking immunoprecipitation (HITS-CLIP) to comprehensively characterize the network of high-confidence, in vivo mRNA:miRNA interactions across white and brown fat, revealing >27,000 unique AGO binding sites. Their work represents the only experimentally generated miRNA targetome in adipose tissue and identifies multiple regulatory pathways that may specify the unique identities of white and brown fat.

CORRIGENDA

Select this article
Corrigendum: H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin
- Ping Chen,
- Jicheng Zhao,
- Yan Wang,
- Min Wang,
- Haizhen Long,
- Dan Liang,
- Li Huang,
- Zengqi Wen,
- Wei Li,
- Xia Li,
- Hongli Feng,
- Haiyong Zhao,
- Ping Zhu,
- Ming Li,
- Qian-fei Wang,
- and Guohong Li
Genes Dev. May 1, 2021 35: 782; doi:10.1101/gad.348504.121
OPEN ACCESS ARTICLE
Select this article
Corrigendum: FGF signaling regulates development by processes beyond canonical pathways
- Ayan T. Ray,
- Pierre Mazot,
- J. Richard Brewer,
- Catarina Catela,
- Colin J. Dinsmore,
- and Philippe Soriano
Genes Dev. May 1, 2021 35: 783; doi:10.1101/gad.348503.121
OPEN ACCESS ARTICLE

REVIEWS

The roles and regulation of MDM2 and MDMX: it is not just about p53

Interfaces between cellular responses to DNA damage and cancer immunotherapy

Establishing and maintaining fertility: the importance of cell cycle arrest

Hepatic transcriptional responses to fasting and feeding

Protein phosphatases in the RNAPII transcription cycle: erasers, sculptors, gatekeepers, and potential drug targets

Building a circuit through correlated spontaneous neuronal activity in the developing vertebrate and invertebrate visual systems

RESEARCH COMMUNICATION

Meikin synergizes with shugoshin to protect cohesin Rec8 during meiosis I

RESEARCH PAPERS

Essential histone chaperones collaborate to regulate transcription and chromatin integrity

MED1 is a lipogenesis coactivator required for postnatal adipose expansion

Critical roles of transcriptional coactivator MED1 in the formation and function of mouse adipose tissues

BAP1 constrains pervasive H2AK119ub1 to control the transcriptional potential of the genome

RESOURCE/METHODOLOGY

AGO HITS-CLIP reveals distinct miRNA regulation of white and brown adipose tissue identity

CORRIGENDA

Corrigendum: H3.3 actively marks enhancers and primes gene transcription via opening higher-ordered chromatin

Corrigendum: FGF signaling regulates development by processes beyond canonical pathways

This Issue

Current Issue

In This Issue