Targeted mutagenesis identifies Asp-569 as a catalytically critical residue in T7 RNA polymerase (Q72153317)

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24 October 2019

title

Targeted mutagenesis identifies Asp-569 as a catalytically critical residue in T7 RNA polymerase (English)

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24 October 2019

6

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24 October 2019

author name string

V O Rechinsky

1

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24 October 2019

B K Chernov

2

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24 October 2019

S M Dragan

3

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24 October 2019

D A Kostyuk

4

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24 October 2019

V L Tunitskaya

5

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24 October 2019

publication date

1 April 1995

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Molecular Genetics and Genomics

24 October 2019

published in

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24 October 2019

volume

247

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24 October 2019

issue

1

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24 October 2019

page(s)

110-113

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Affinity modification of DNA-dependent RNA-polymerase of phage T7 with 5'-p-fluorosulfonylbenzoyladenosine

24 October 2019

cites work

1 reference

https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Lys631 residue in the active site of the bacteriophage T7 RNA polymerase. Affinity labeling and site-directed mutagenesis

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Site-specific mutagenesis of residue Lys-172 of phage T7 RNA polymerase: characterization of transcription properties of mutant proteins

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Mutations in T7 RNA polymerase that support the proposal for a common polymerase active site structure

1 reference

https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

[Functional studies of mutant forms of bacteriophage T7 RNA polymerases containing point substitutions in the motif at the active site of the enzyme]

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Rapid and efficient site-specific mutagenesis without phenotypic selection

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

An attempt to unify the structure of polymerases

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

T7 promoter contacts essential for promoter activityin vivo

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

The phage RNA polymerases are related to DNA polymerases and reverse transcriptases

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Random mutagenesis of the gene for bacteriophage T7 RNA polymerase

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Crystal structure of bacteriophage T7 RNA polymerase at 3.3 Å resolution

1 reference

https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Asp537, Asp812 are essential and Lys631, His811 are catalytically significant in bacteriophage T7 RNA polymerase activity

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Isolation and characterization of mutant bacteriophage T7 RNA polymerases

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

DNA sequence analysis with a modified bacteriophage T7 DNA polymerase

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Characterization of bacteriophage T7 RNA polymerase by linker insertion mutagenesis

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Tyr-571 is involved in the T7 RNA polymerase binding to its promoter

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https://pubmed.ncbi.nlm.nih.gov/7715597

12 December 2020

Identifiers

DOI

10.1007/BF00425827

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24 October 2019

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