Detection of T-cell degranulation: CD107a and b. (Q45189688)

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6 January 2020

title

Detection of T-cell degranulation: CD107a and b (English)

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6 January 2020

2

Richard A Koup

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6 January 2020

1

Michael R Betts

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6 January 2020

publication date

1 January 2004

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6 January 2020

published in

Methods in Cell Biology

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6 January 2020

volume

75

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6 January 2020

page(s)

497-512

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Phenotypic analysis of antigen-specific T lymphocytes

6 January 2020

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HIV-specific CD8(+) T cells produce antiviral cytokines but are impaired in cytolytic function

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Cytotoxic T lymphocytes: all roads lead to death

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Sensitive and viable identification of antigen-specific CD8+ T cells by a flow cytometric assay for degranulation

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Putative immunodominant human immunodeficiency virus-specific CD8(+) T-cell responses cannot be predicted by major histocompatibility complex class I haplotype

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Degranulation plays an essential part in regulating cell surface expression of Fas ligand in T cells and natural killer cells

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Retrieval of TGN proteins from the cell surface requires endosomal acidification.

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At the acidic edge: emerging functions for lysosomal membrane proteins

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An improved flow cytometric assay for the determination of cytotoxic T lymphocyte activity

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Different steady state subcellular distributions of the three splice variants of lysosome-associated membrane protein LAMP-2 are determined largely by the COOH-terminal amino acid residue

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Protein sorting and secretion during CTL killing

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Lymphocyte-mediated cytotoxicity: two pathways and multiple effector molecules

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Cytoplasmic determinants involved in direct lysosomal sorting, endocytosis, and basolateral targeting of rat lgp120 (lamp-I) in MDCK cells.

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Detection of intracellular cytokines by flow cytometry

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Lysosome-associated membrane proteins h-LAMP1 (CD107a) and h-LAMP2 (CD107b) are activation-dependent cell surface glycoproteins in human peripheral blood mononuclear cells which mediate cell adhesion to vascular endothelium

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T-cell epitope mapping by flow cytometry

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A novel flow cytometric assay for quantitation and multiparametric characterization of cell-mediated cytotoxicity

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Strategies for phenotyping apoptotic peripheral human lymphocytes comparing ISNT, annexin-V and 7-AAD cytofluorometric staining methods

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Visualization and quantification of T cell-mediated cytotoxicity using cell-permeable fluorogenic caspase substrates

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Alteration of intracellular traffic by monensin; mechanism, specificity and relationship to toxicity

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Cytotoxic T lymphocyte granules are secretory lysosomes, containing both perforin and granzymes

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Dendritic cell elimination as an assay of cytotoxic T lymphocyte activity in vivo

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Kinetics and temperature dependence of exposure of endocytosed material to proteolytic enzymes and low pH: evidence for a maturation model for the formation of lysosomes

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Ex vivo identification, isolation and analysis of tumor-cytolytic T cells.

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Target cell lysis by CTL granule exocytosis is independent of ICE/Ced-3 family proteases

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A novel technique for the fluorometric assessment of T lymphocyte antigen specific lysis

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Efficient Nuclear Targeting of Granzyme B and the Nuclear Consequences of Apoptosis Induced by Granzyme B and Perforin Are Caspase-dependent, but Cell Death Is Caspase-independent

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Determination of antigen-specific memory/effector CD4+ T cell frequencies by flow cytometry: evidence for a novel, antigen-specific homeostatic mechanism in HIV-associated immunodeficiency

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7 January 2021

10.1016/S0091-679X(04)75020-7

Identifiers

DOI

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6 January 2020

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