Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing (Q40503492)

21 September 2017

Assembling a Correctly Folded and Functional Heptahelical Membrane Protein by Protein Trans-splicing (English)

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21 September 2017

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9

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21 September 2017

Volker Dötsch

8

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Josef Wachtveitl

object named as

Josef Wachtveitl

7

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author name string

Michaela Mehler

1

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21 September 2017

Carl Elias Eckert

2

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21 September 2017

Alena Busche

3

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21 September 2017

Jennifer Kulhei

4

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21 September 2017

Jonas Michaelis

5

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21 September 2017

Johanna Becker-Baldus

6

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21 September 2017

language of work or name

English

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publication date

24 September 2015

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Journal of Biological Chemistry

21 September 2017

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21 September 2017

290

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21 September 2017

46

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21 September 2017

27712-27722

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Folding energetics and oligomerization of polytopic α-helical transmembrane proteins

21 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Cross-protomer interaction with the photoactive site in oligomeric proteorhodopsin complexes

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Solution NMR Structure of Proteorhodopsin

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Protein trans-splicing and its use in structural biology: opportunities and limitations.

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Reconstitution of modular PDK1 functions on trans-splicing of the regulatory PH and catalytic kinase domains

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

TOTAPOL: a biradical polarizing agent for dynamic nuclear polarization experiments in aqueous media

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Color Tuning in Rhodopsins: The Mechanism for the Spectral Shift between Bacteriorhodopsin and Sensory Rhodopsin II

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

SUMO fusions and SUMO-specific protease for efficient expression and purification of proteins.

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Novel Proteorhodopsin variants from the Mediterranean and Red Seas.

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Characterization of the photochemical reaction cycle of proteorhodopsin

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Proteorhodopsin is a light-driven proton pump with variable vectoriality

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Proton transfers in the photochemical reaction cycle of proteorhodopsin

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Detection of protein-protein interactions in vivo based on protein splicing

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Bacterial rhodopsin: evidence for a new type of phototrophy in the sea

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Helical membrane protein folding, stability, and evolution

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Structure of bacteriorhodopsin at 1.55 A resolution

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Expressed protein ligation: a general method for protein engineering

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Protein splicing elements: inteins and exteins--a definition of terms and recommended nomenclature

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

In vivo assembly of rhodopsin from expressed polypeptide fragments

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

The EF loop in green proteorhodopsin affects conformation and photocycle dynamics

22 September 2017

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Mechanisms of spectral tuning in blue cone visual pigments. Visible and raman spectroscopy of blue-shifted rhodopsin mutants

17 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Structural basis of the green-blue color switching in proteorhodopsin as determined by NMR spectroscopy

17 June 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Unambiguous assignment of short- and long-range structural restraints by solid-state NMR spectroscopy with segmental isotope labeling

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Faster protein splicing with the Nostoc punctiforme DnaE intein using non-native extein residues

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Low temperature FTIR spectroscopy provides new insights in the pH-dependent proton pathway of proteorhodopsin

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

His75-Asp97 cluster in green proteorhodopsin

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Functional reconstitution of the human serotonin receptor 5-HT(6) using synthetic transmembrane peptides

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Site-specific two-color protein labeling for FRET studies using split inteins.

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Segmental isotopic labeling of a central domain in a multidomain protein by protein trans-splicing using only one robust DnaE intein.

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Expanding the scope of protein trans-splicing to fragment ligation of an integral membrane protein: towards modulation of porin-based ion channels by chemical modification

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

The naturally split Npu DnaE intein exhibits an extraordinarily high rate in the protein trans-splicing reaction

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Three-dimensional solid-state NMR study of a seven-helical integral membrane proton pump--structural insights

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

His-75 in proteorhodopsin, a novel component in light-driven proton translocation by primary pumps

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Color change of proteorhodopsin by a single amino acid replacement at a distant cytoplasmic loop.

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Characterisation of Schiff base and chromophore in green proteorhodopsin by solid-state NMR.

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Intein-based biosynthetic incorporation of unlabeled protein tags into isotopically labeled proteins for NMR studies.

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Construction and in vivo analysis of new split lactose permeases.

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Information content in the circular dichroism of proteins.

20 August 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=4646914

Highly efficient proteintrans-splicing by a naturally split DnaE intein fromNostoc punctiforme

20 August 2018

1 reference

Crossref

https://api.crossref.org/works/10.1074%2FJBC.M115.681205

Refolding of bacteriorhodopsin from expressed polypeptide fragments

21 January 2018

1 reference

https://pubmed.ncbi.nlm.nih.gov/26405032

12 December 2020

Refolding of an integral membrane protein. Denaturation, renaturation, and reconstitution of intact bacteriorhodopsin and two proteolytic fragments

1 reference

https://pubmed.ncbi.nlm.nih.gov/26405032

12 December 2020

Refolding of bacteriorhodopsin in lipid bilayers. A thermodynamically controlled two-stage process

1 reference

https://pubmed.ncbi.nlm.nih.gov/26405032

12 December 2020

An Improved Broadband Decoupling Sequence for Liquid Crystals and Solids

1 reference

https://pubmed.ncbi.nlm.nih.gov/26405032

12 December 2020

Split-receptors in the tachykinin neurokinin-1 system--mutational analysis of intracellular loop 3

1 reference

https://pubmed.ncbi.nlm.nih.gov/26405032

12 December 2020

Identifiers

DOI

10.1074/JBC.M115.681205

1 reference

21 September 2017

1 reference

21 September 2017

PubMed publication ID

26405032

1 reference