Strategies for the cloning and expression of membrane proteins (Q37776038)

23 August 2017

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title

Strategies for the cloning and expression of membrane proteins (English)

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23 August 2017

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Christopher M M Koth

1

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23 August 2017

Jian Payandeh

series ordinal

2

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23 August 2017

language of work or name

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27 November 2009

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Advances in protein chemistry and structural biology

23 August 2017

published in

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23 August 2017

volume

76

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23 August 2017

page(s)

43-86

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Crystal structure of a human membrane protein involved in cysteinyl leukotriene biosynthesis

23 August 2017

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7 January 2021

Crystal Structure of a Yeast Aquaporin at 1.15 Å Reveals a Novel Gating Mechanism

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Rapid one-step recombinational cloning

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Quality control of overexpressed membrane proteins

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Expression of the Human Oxytocin Receptor in Baculovirus-Infected Insect Cells: High-Affinity Binding Is Induced by a Cholesterol-Cyclodextrin Complex

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Large-scale production of functional membrane proteins

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The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation

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The channelopathies: novel insights into molecular and genetic mechanisms of human disease

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Structure and function in rhodopsin: the role of asparagine-linked glycosylation

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Fluorescence-detection size-exclusion chromatography for precrystallization screening of integral membrane proteins

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Crystal structure of the ATP-gated P2X(4) ion channel in the closed state

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Mechanism of ammonia transport by Amt/MEP/Rh: structure of AmtB at 1.35 A.

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Expression of an olfactory receptor in Escherichia coli: purification, reconstitution, and ligand binding.

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Refolding of G-protein-coupled receptors from inclusion bodies produced in Escherichia coli

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7 January 2021

Cell-free production of G protein-coupled receptors for functional and structural studies.

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7 January 2021

Combination of cell-free expression and NMR spectroscopy as a new approach for structural investigation of membrane proteins

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Structure-based discovery of beta2-adrenergic receptor ligands

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7 January 2021

Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli

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Expression of membrane proteins from Mycobacterium tuberculosis in Escherichia coli as fusions with maltose binding protein

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Expression and purification of human TRPV1 in baculovirus-infected insect cells for structural studies.

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7 January 2021

Baculovirus as versatile vectors for protein expression in insect and mammalian cells

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Determination and application of empirically derived detergent phase boundaries to effectively crystallize membrane proteins

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7 January 2021

Identification of ligands for olfactory receptors by functional expression of a receptor library

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7 January 2021

Bacterial expression of functional, biotinylated peripheral cannabinoid receptor CB2.

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Lactococcus lactis as host for overproduction of functional membrane proteins

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7 January 2021

Dynamic oligomeric conversions of the cytoplasmic RCK domains mediate MthK potassium channel activity

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Patch clamp and phenotypic analyses of a prokaryotic cyclic nucleotide-gated K+ channel using Escherichia coli as a host

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Development of a minimal cell-free translation system for the synthesis of presecretory and integral membrane proteins.

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Role of sterols in modulating the human mu-opioid receptor function in Saccharomyces cerevisiae

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The funnel approach to the precrystallization production of membrane proteins

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A P-type ATPase importer that discriminates between essential and toxic transition metals

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Selecting optimum eukaryotic integral membrane proteins for structure determination by rapid expression and solubilization screening

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7 January 2021

Efficient production of membrane-integrated and detergent-soluble G protein-coupled receptors in Escherichia coli

1 reference

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7 January 2021

Structure of a tetrameric MscL in an expanded intermediate state

1 reference

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7 January 2021

The E. coli BtuCD structure: a framework for ABC transporter architecture and mechanism

1 reference

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7 January 2021

Crystal structure of a mammalian voltage-dependent Shaker family K+ channel

1 reference

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7 January 2021

Atomic structure of a voltage-dependent K+ channel in a lipid membrane-like environment

1 reference

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7 January 2021

Exploring the activity of tobacco etch virus protease in detergent solutions

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7 January 2021

Structural genomics of GPCRs

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7 January 2021

Structural genomics on membrane proteins: comparison of more than 100 GPCRs in 3 expression systems.

1 reference

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7 January 2021

Crystal structure of the CorA Mg2+ transporter

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7 January 2021

Structure of the connexin 26 gap junction channel at 3.5 A resolution

1 reference

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7 January 2021

Co-evolving stability and conformational homogeneity of the human adenosine A2a receptor

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7 January 2021

Expression of recombinant G-protein coupled receptors for structural biology

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7 January 2021

Many paths to many clones: a comparative look at high-throughput cloning methods

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7 January 2021

Pressure-sensitive ion channel in Escherichia coli

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7 January 2021

Engineering membrane protein overproduction in Escherichia coli

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7 January 2021

Structural basis for synthesis of inflammatory mediators by human leukotriene C4 synthase

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7 January 2021

Genetic selection system for improving recombinant membrane protein expression in E. coli

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7 January 2021

Sterol and lipid trafficking in mammalian cells

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7 January 2021

Heterologous GPCR expression: a bottleneck to obtaining crystal structures

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7 January 2021

Mammalian G-protein-coupled receptor expression in Escherichia coli: I. High-throughput large-scale production as inclusion bodies.

1 reference

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7 January 2021

Breaking the bottleneck: eukaryotic membrane protein expression for high-resolution structural studies.

1 reference

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7 January 2021

Reconstruction of the P2X(2) receptor reveals a vase-shaped structure with lateral tunnels above the membrane.

1 reference

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7 January 2021

Over-production of proteins in Escherichia coli: mutant hosts that allow synthesis of some membrane proteins and globular proteins at high levels

1 reference

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7 January 2021

Inhibition of tobacco etch virus protease activity by detergents

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7 January 2021

Membrane protein expression and production: effects of polyhistidine tag length and position

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7 January 2021

Functional expression of eukaryotic membrane proteins in Lactococcus lactis.

1 reference

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7 January 2021

Crystal structure of squid rhodopsin

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7 January 2021

Eukaryotic integral membrane protein expression utilizing the Escherichia coli glycerol-conducting channel protein (GlpF).

1 reference

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7 January 2021

Crystal structure of the aquaglyceroporin PfAQP from the malarial parasite Plasmodium falciparum

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7 January 2021

A general protocol for the crystallization of membrane proteins for X-ray structural investigation

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7 January 2021

High-throughput fluorescent-based optimization of eukaryotic membrane protein overexpression and purification in Saccharomyces cerevisiae

1 reference

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7 January 2021

Reconstitution of the transferrin receptor in lipid vesicles. Effect of cholesterol on the binding of transferrin

1 reference

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7 January 2021

Crystal structure of rhodopsin: implications for vision and beyond

1 reference

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7 January 2021

Specific lipid requirements of membrane proteins--a putative bottleneck in heterologous expression

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7 January 2021

The structure of phospholamban pentamer reveals a channel-like architecture in membranes

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7 January 2021

Co-axial association of recombinant eye lens aquaporin-0 observed in loosely packed 3D crystals.

1 reference

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7 January 2021

G protein-coupled receptor rhodopsin

1 reference

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7 January 2021

Crystal structure of rhodopsin: A G protein-coupled receptor

1 reference

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7 January 2021

Crystal structure of the ligand-free G-protein-coupled receptor opsin

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7 January 2021

Crystal structure of the plasma membrane proton pump.

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7 January 2021

Seven-transmembrane receptors

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7 January 2021

An inward-facing conformation of a putative metal-chelate-type ABC transporter

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7 January 2021

Studying membrane proteins through the eyes of the genetic code revealed a strong uracil bias in their coding mRNAs.

1 reference

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7 January 2021

Expression screening of integral membrane proteins from Helicobacter pylori 26695.

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7 January 2021

AcrB et al.: Obstinate contaminants in a picogram scale. One more bottleneck in the membrane protein structure pipeline.

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7 January 2021

Construction of a series of vectors for high throughput cloning and expression screening of membrane proteins from Mycobacterium tuberculosis

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7 January 2021

Crystal structure of the human beta2 adrenergic G-protein-coupled receptor

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7 January 2021

High-level production, solubilization and purification of synthetic human GPCR chemokine receptors CCR5, CCR3, CXCR4 and CX3CR1

1 reference

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7 January 2021

Investigation of the biophysical and cell biological properties of ferroportin, a multipass integral membrane protein iron exporter

1 reference

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7 January 2021

NMR structure of Mistic, a membrane-integrating protein for membrane protein expression

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7 January 2021

GPCR engineering yields high-resolution structural insights into beta2-adrenergic receptor function

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7 January 2021

Stabilization of the human beta2-adrenergic receptor TM4-TM3-TM5 helix interface by mutagenesis of Glu122(3.41), a critical residue in GPCR structure

1 reference

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7 January 2021

Structures of membrane proteins determined at atomic resolution

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7 January 2021

Directed evolution of a G protein-coupled receptor for expression, stability, and binding selectivity

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7 January 2021

Cell-free complements in vivo expression of the E. coli membrane proteome

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7 January 2021

Crystal structure of opsin in its G-protein-interacting conformation

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7 January 2021

Structure and mechanism of the M2 proton channel of influenza A virus

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7 January 2021

Production of membrane proteins using cell-free expression systems

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7 January 2021

Preparative scale expression of membrane proteins in Escherichia coli-based continuous exchange cell-free systems.

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7 January 2021

Crucial steps in the structure determination of the Na+/H+ antiporter NhaA in its native conformation

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7 January 2021

Conformational thermostabilization of the beta1-adrenergic receptor in a detergent-resistant form

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7 January 2021

Atomic structure of a Na+- and K+-conducting channel

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7 January 2021

Disposable bioreactor for cell culture using wave-induced agitation

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7 January 2021

Engineering G protein-coupled receptor expression in bacteria

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7 January 2021

Expression of delta, kappa and mu human opioid receptors in Escherichia coli and reconstitution of the high-affinity state for agonist with heterotrimeric G proteins

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7 January 2021

Crystal Structure of a Thermally Stable Rhodopsin Mutant

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7 January 2021

Helical extension of the neuronal SNARE complex into the membrane

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7 January 2021

2007 annual progress report synopsis of the Center for Structures of Membrane Proteins.

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7 January 2021

Protein production by auto-induction in high density shaking cultures

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7 January 2021

Use of T7 RNA polymerase to direct expression of cloned genes

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7 January 2021

Fluorescence-based optimization of human bitter taste receptor expression in Saccharomyces cerevisiae

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7 January 2021

Comparative analysis and "expression space" coverage of the production of prokaryotic membrane proteins for structural genomics.

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7 January 2021

Mechanism of aquaporin-4's fast and highly selective water conduction and proton exclusion

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7 January 2021

Constitutive activation of G protein-coupled receptors and diseases: insights into mechanisms of activation and therapeutics

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7 January 2021

Comparison of seven different heterologous protein expression systems for the production of the serotonin transporter

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7 January 2021

Functional expression of heterologous proteins in yeast: insights into Ca2+ signaling and Ca2+-transporting ATPases

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7 January 2021

Structural mechanism of plant aquaporin gating

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7 January 2021

Purification of a rat neurotensin receptor expressed in Escherichia coli

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7 January 2021

Reconstitution of intramembrane proteolysis in vitro reveals that pure rhomboid is sufficient for catalysis and specificity

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7 January 2021

X-ray structure of a protein-conducting channel

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7 January 2021

There is a baby in the bath water: AcrB contamination is a major problem in membrane-protein crystallization

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7 January 2021

Consequences of membrane protein overexpression in Escherichia coli.

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7 January 2021

Rationalizing membrane protein overexpression

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7 January 2021

Tuning Escherichia coli for membrane protein overexpression

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7 January 2021

GATEWAY recombinational cloning: application to the cloning of large numbers of open reading frames or ORFeomes.

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7 January 2021

Practical aspects of overexpressing bacterial secondary membrane transporters for structural studies.

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7 January 2021

The Structure of an Open Form of an E. coli Mechanosensitive Channel at 3.45 A Resolution

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7 January 2021

Structure of a beta1-adrenergic G-protein-coupled receptor

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7 January 2021

Development and crystallization of a minimal thermostabilised G protein-coupled receptor.

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7 January 2021

Making the most of affinity tags

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7 January 2021

Purification and characterization of the human adenosine A(2a) receptor functionally expressed in Escherichia coli

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7 January 2021

Characteristics affecting expression and solubilization of yeast membrane proteins

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7 January 2021

Functional expression of rat alpha 2B-adrenoceptor in Escherichia coli

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7 January 2021

Use of dual affinity tags for expression and purification of functional peripheral cannabinoid receptor

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7 January 2021

Structure of a glutamate transporter homologue from Pyrococcus horikoshii

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7 January 2021

Comparison of functional non-glycosylated GPCRs expression in Pichia pastoris

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7 January 2021

Dot-blot immunodetection as a versatile and high-throughput assay to evaluate recombinant GPCRs produced in the yeast Pichia pastoris

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7 January 2021

Transient ribosomal attenuation coordinates protein synthesis and co-translational folding.

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7 January 2021

Chemistry of ion coordination and hydration revealed by a K+ channel-Fab complex at 2.0 A resolution

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7 January 2021

10.1016/S1876-1623(08)76002-4

Identifiers

DOI

1 reference

23 August 2017

1 reference