Intersubunit signaling in RecBCD enzyme, a complex protein machine regulated by Chi hot spots (Q24680851)

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Andrew F Taylor

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Manjula Reddy

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Gerald R Smith

describes a project that uses

4

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language of work or name

English

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publication date

15 December 2007

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published in

Genes & Development

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volume

21

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issue

24

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page(s)

3296-307

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ImageQuant

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Europe PubMed Central

11 June 2022

https://www.ebi.ac.uk/europepmc/webservices/rest/PMC2113030/fullTextXML

inferred from PubMed Central ID database lookup

cites work

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Regulation of homologous recombination: Chi inactivates RecBCD enzyme by disassembly of the three subunits

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RecBCD enzyme is altered upon cutting DNA at a chi recombination hotspot

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A molecular throttle: the recombination hotspot chi controls DNA translocation by the RecBCD helicase

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RecBCD enzyme is a DNA helicase with fast and slow motors of opposite polarity

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A domain of RecC required for assembly of the regulatory RecD subunit into the Escherichia coli RecBCD holoenzyme

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The RecD subunit of the Escherichia coli RecBCD enzyme inhibits RecA loading, homologous recombination, and DNA repair

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The RecBC enzyme loads RecA protein onto ssDNA asymmetrically and independently of chi, resulting in constitutive recombination activation

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The translocating RecBCD enzyme stimulates recombination by directing RecA protein onto ssDNA in a chi-regulated manner

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The recombination hotspot Chi is recognized by the translocating RecBCD enzyme as the single strand of DNA containing the sequence 5'-GCTGGTGG-3'.

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The recombination hot spot chi is a regulatory element that switches the polarity of DNA degradation by the RecBCD enzyme

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The recombination hotspot chi is a regulatory sequence that acts by attenuating the nuclease activity of the E. coli RecBCD enzyme

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Strand specificity of nicking of DNA at Chi sites by RecBCD enzyme. Modulation by ATP and magnesium levels

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Monomeric RecBCD Enzyme Binds and Unwinds DNA

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RecBC enzyme nicking at Chi sites during DNA unwinding: location and orientation-dependence of the cutting

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28 September 2017

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Homologous pairing in vitro stimulated by the recombination Hotspot, Chi

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Alteration by site-directed mutagenesis of the conserved lysine residue in the ATP-binding consensus sequence of the RecD subunit of the Escherichia coli RecBCD enzyme

28 September 2017

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28 September 2017

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On the role of ATP in phosphodiester bond hydrolysis catalyzed by the recBC deoxyribonuclease of Escherichia coli

28 September 2017

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A novel, 11 nucleotide variant of chi, chi*: one of a class of sequences defining the Escherichia coli recombination hotspot chi.

28 September 2017

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A single mutation, RecB(D1080A,) eliminates RecA protein loading but not Chi recognition by RecBCD enzyme

30 May 2018

1 reference

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The RecBCD enzyme initiation complex for DNA unwinding: enzyme positioning and DNA opening.

30 May 2018

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Distribution of Chi-stimulated recombinational exchanges and heteroduplex endpoints in phage lambda

30 May 2018

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The RecA binding locus of RecBCD is a general domain for recruitment of DNA strand exchange proteins

30 May 2018

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Translocation by the RecB motor is an absolute requirement for {chi}-recognition and RecA protein loading by RecBCD enzyme

28 November 2018

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Direct visualization of RecBCD movement reveals cotranslocation of the RecD motor after chi recognition

28 November 2018

1 reference

https://eutils.ncbi.nlm.nih.gov/entrez/eutils/elink.fcgi?dbfrom=pmc&linkname=pmc_refs_pubmed&retmode=json&id=2113030

The cell as a collection of protein machines: preparing the next generation of molecular biologists

28 November 2018

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Strand-specific binding to duplex DNA ends by the subunits of the Escherichia coli RecBCD enzyme.

28 November 2018

1 reference

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χ-Sequence recognition and DNA translocation by single RecBCD helicase/nuclease molecules

28 November 2018

1 reference

https://pubmed.ncbi.nlm.nih.gov/18079176

12 December 2020

Protection of parental T4 DNA from a restriction exonuclease by the product of gene 2

1 reference

https://pubmed.ncbi.nlm.nih.gov/18079176

12 December 2020

Structure of chi hotspots of generalized recombination

1 reference

https://pubmed.ncbi.nlm.nih.gov/18079176

12 December 2020

Site-directed mutations in motif VI of Escherichia coli DNA helicase II result in multiple biochemical defects: evidence for the involvement of motif VI in the coupling of ATPase and DNA binding activities via conformational changes

1 reference

https://pubmed.ncbi.nlm.nih.gov/18079176

12 December 2020

Identification of the nuclease active site in the multifunctional RecBCD enzyme by creation of a chimeric enzyme

1 reference

https://pubmed.ncbi.nlm.nih.gov/18079176

12 December 2020

Identifiers

DOI

10.1101/GAD.1605807

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1 reference

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PubMed publication ID

18079176

1 reference