Determination of The Alcohol Lab

AP Chemistry
Determination of the Alcohol Content of Root

Beer Lab
Background:
This analysis determines the alcohol content of root beer by a back titration method. The
ethanol is oxidized to ethanoic acid by heating with an excess of acidified potassium
dichromate solution.
3C2H5OH + 2Cr2O72- + 16H+ 3CH3COOH + 4Cr3+ 11H2O (Eqn 1)
Excess KI is added and this reacts with any remaining Cr2O72- - to produce iodine.
Cr2O72- + 14H+ + 6I- 2Cr3+ + 7H2O + 3I2 (Eqn 2)
The iodine produced is then titrated with standard sodium thiosulfate solution.
I2 + 2S2O32- 2I- + S4O62-- (Eqn 3)
The indicator used in this titration is a 1% solution of starch
For the determination of alcohol a standardized solution of sodium thiosulfate is required.
The sodium thiosulfate solution concentration is determined by titration with a known
potassium iodate solution. The standardization solution is made by adding solid KI to
known concentration solution of sodium thiosulfate. The following is the reaction that
occurs:
IO3 - + 5I- + 6 H+ 3 I2 + 3 H2O (Eqn 4)
The iodine is then titrated with the sodium thiosulfate solution to endpoint and the moles
of thiosulfate calculated. This known solution is used to determine the alcohol content of
the root beer by titrating and using equations 1 to 3.
Solutions:
0.05 mol L-1 potassium dichromate (CAUTION hazardous substance)
50% sulfuric acid (CAUTION- highly corrosive)
0.5 mol L-1 KI
0.1 mol L-1 sodium thiosulfate
1% starch solution made by adding 100mL of boiling water to 1 g of starch powder.
Standardization of 0.10 M sodium thiosulfate:
1. Pipette 50.00 mL of the 0.01 M KIO3 solution into a 250-mL flask.
2. Add 2 g of solid KI and 10 mL of 0.5 M H2SO4.
3. Immediately titrate with thiosulfate until the solution has lost its initial reddishbrown color and has become pale yellow.
4. Add 2 mL of starch indicator and complete the titration.
5. Share the data of your one trial with the other groups. All the groups need to
calculate the average concentration for the class and use that average
concentration to calculate the amount of alcohol in the root beer.
1
Revised: 17 Jan 2010
Determination of Alcohol Content of Root Beer Lab
Procedure
Record the method used (A or B) and whether the initial sample of root beer is diluted
or not in your data table.
Method A:
1. Pipette 10 mL of root beer into a 250 mL volumetric flask and make up to the
mark with water.
2. Place a 5 mL aliquot of the diluted root beer in a 150 mL Erlenmeyer flask and
add 10 mL of 0.05 mol L-1 potassium dichromate. Slowly add about 20 mL of
50% sulfuric acid solution to each flask. WARNING This solution is very
corrosive.
3. Stopper each flask loosely and heat in a water bath, at no more than 50C, for at
least 60 minutes.
4. Remove from the water bath and add about 10 mL of 0.5 mol L-1 KI.
5. Titrate the contents with 0.1 mol L-1 sodium thiosulfate solution. When the brown
color of the solution gets a green tinge, add a few drops of starch indicator.
Continue adding thiosulfate solution until the solution goes a clear, green-blue
color. This is the endpoint of the titration- it is not easy to detect, but with
practice it will become easier.
1. NOTE: It is possible to check whether the endpoint has been reached by holding
the solution in the sunlight and adding a drop of starch indicator. The appearance
of a dark blue color in the solution (where the drop was added) indicates iodine is
still present.
Method B:
If the alcohol sample being investigated
contains significant quantities of other
substances that are able to be oxidized by
acidified dichromate then the value obtained
will be too high. This commonly occurs with
alcoholic drinks such as beers and wines
because of the presence of other organic
molecules such as secondary alcohols. In
these situations it would usually be necessary
to separate the alcohol from the sample by
fractional distillation which is difficult and time consuming.
However, if the interfering substances are less volatile than the alcohol itself the
following modification known as Nicholls diffusion works adequately (as described in
CHEMNZ No 73, Nov 98 pp 21-22). In this method, instead of adding the alcohol
sample directly to the acidified dichromate mixture it is placed in a small container and
suspended above the dichromate solution and left to diffuse into the solution where it is
oxidized as previously described. The other components of the alcohol sample remain
behind in the sample container and so do not contribute to the oxidation process. A
diagram of the experimental set up is shown at right. Because the suspended container is
2
Determination of Alcohol Content of Root Beer Lab

of limited volume, smaller quantities must be used which usually means the original
alcohol sample is not diluted before use.
Method B:
1. Pipette 10 mL of root into a 100 mL volumetric flask and make up to the mark
with distilled water.
2. Use a graduated pipette to accurately add 2 mL of the root beer sample into the
small test tube, ensure that the test tube will fit into the 250 mL Erlenmeyer flask.
NOTE: The quantity required will depend on the sample, e.g. for beers and
mixers about 5 mL of a 10% solution should be appropriate.
3. Pipette 10 mL of the 0.05 mol L-1 potassium dichromate. Slowly add about 20
mL of 50% sulfuric acid solution to each flask. WARNING -This solution is very
corrosive.
4. Stopper each flask loosely and heat in a water bath, at no more than 50C, for a
few hours or overnight.
5. Remove from the water bath and carefully remove the suspended container
without spilling its contents.
6. Add about 10 mL of 0.5 mol L-1 KI into the flask containing the acidified
dichromate.
7. Titrate the contents with 0.1 mol L-1 sodium thiosulfate solution. When the brown
color of the solution gets a green tinge, add a few drops of starch indicator.
Continue adding thiosulfate solution until the solution goes a clear, green-blue
color.
8. Using the titration data determine the number of moles of Cr2O72- remaining in the
flask. By subtracting this from the total amount of dichromate originally added to
the flask it is possible to determine how many moles of dichromate have reacted
with the ethanol present in the original sample. The concentration of ethanol can
then be determined in either mol L-1, g L-1 or %(V/V).

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AP Chemistry

Determination of the Alcohol Content of Root

Determination of Alcohol Content of Root Beer Lab

Determination of Alcohol Content of Root Beer Lab

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